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EBI DbfetchID AF534914; SV 1; linear; genomic DNA; STD; PRO; 4302 BP. XX AC AF534914; XX DT 01-SEP-2005 (Rel. 85, Created) DT 01-SEP-2005 (Rel. 85, Last updated, Version 1) XX DE Pseudomonas putida OrcA (orcA) gene, partial cds; OrcB (orcB), OrcC (orcC), DE and OrcD (orcD) genes, complete cds; and OrcF (orcF) gene, partial cds. XX KW . XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-4302 RA Straganz G.D., Glieder A., Steiner W.; RT "Cloning of Genes Involved in Meta-Cleavage Pathways of Pseudomonas putida RT ORC: Nucleotide Sequences of Genes, Characterization of Two Dioxygenases RT and Identification of the Orcinol Pathway"; RL Unpublished. XX RN [2] RP 1-4302 RA Straganz G.D., Glieder A., Steiner W.; RT ; RL Submitted (06-AUG-2002) to the EMBL/GenBank/DDBJ databases. RL Institute of Biotechnology, University of Technology, Petersgasse 12, Graz, RL Styria 8010, Austria XX FH Key Location/Qualifiers FH FT source 1..4302 FT /organism="Pseudomonas putida" FT /strain="ORC" FT /mol_type="genomic DNA" FT /note="methylcatechol cleaving clone with potential to grow FT on orcinol as sole carbon source" FT /db_xref="taxon:303" FT gene <1..1075 FT /gene="orcA" FT CDS <1..1075 FT /codon_start=2 FT /transl_table=11 FT /gene="orcA" FT /product="OrcA" FT /note="similar to orcinol monooxygenase" FT /db_xref="GOA:Q400K5" FT /db_xref="InterPro:IPR003042" FT /db_xref="UniProtKB/TrEMBL:Q400K5" FT /protein_id="AAQ10533.1" FT /translation="IVIDLANDPLGTPNVYLCCDPVRPYVSAALPHGVRRFEFMVMPGE FT TEEELGKPENLRQLLAKVLPDPDSVELIRKRVYTHNARLAGQFRVKRVLLAGDAAHIMP FT VWQGQGYNSGMRDAGNLAWKLALVVKGLAGEALLDTYEQERRAHAKAMIDLSVLAGHVL FT APPKRWQGALRDGISWLLNYLPPVKRYFLEMRFKPMPQYARGALVSEQSGKASPVGRLF FT IQPKVLTEAGETLLLDEWIGSNFAVIAWGNDPLWGLSREQVEAWRALGTRFIQVLPEVQ FT LKAGREVPEGVIRVGDSTGRLKEWFGRYPASIALLRPDRFVAGVAIPQSLGQVGDQLLR FT ALAAIPQLSSAAGVQVA" FT gene 1077..2021 FT /gene="orcB" FT CDS 1077..2021 FT /codon_start=1 FT /transl_table=11 FT /gene="orcB" FT /product="OrcB" FT /note="extradiol ring cleaving dioxygenase; catechol FT dioxygenase induced during orcinol degradation; second FT enzyme of the orcinol metabolism" FT /db_xref="GOA:Q400K4" FT /db_xref="InterPro:IPR004183" FT /db_xref="UniProtKB/Swiss-Prot:Q400K4" FT /protein_id="AAQ10534.1" FT /translation="MSAYLHCLSHTPLVGYVDPVAEVLAEVDEVVAAARARIAAFDPQL FT VFLFAPDHYNGFFYDVMPSFCIGMAATAIGDFHSLAGPLDVPRETAEACAAAVLEAGVD FT AAVSYRMQVDHGFAQPLELLLGGLAEKPVVPVFINGVAVPLPGFQRARLLGEAIGRFAR FT STGKRVLFLGSGGLSHQPPVPELAKVDARMADRLLGSGRDLPTDERQARQQRVISAAEG FT FVADQNSLHPLNPEWDNHFLDLLEQRRFAELDGLGNAELSALAGKSTHEVKTWVTAFAA FT LSAFGPYQAHERYYRPIPEWIAGFGSLSAHSLS" FT gene 2037..2897 FT /gene="orcC" FT CDS 2037..2897 FT /codon_start=1 FT /transl_table=11 FT /gene="orcC" FT /product="OrcC" FT /note="C-C bond cleaving hydrolase; third hydrolytic enzyme FT of the meta cleavage pathway of orcinol degradation" FT /db_xref="GOA:Q400K3" FT /db_xref="InterPro:IPR000639" FT /db_xref="UniProtKB/Swiss-Prot:Q400K3" FT /protein_id="AAQ10535.1" FT /translation="MTIDISEASSSRFARIREGELDLQLHYNDLGEGAETVVMLHGSGP FT GASGWANFSRNLEPLLAAGYRVVLMDCPGWSKSDPIVCRSSRSDLNATALKGLLDMLGL FT ERVHILGNSMGAHSAVAFALANPRRVGKLVLMGGGTGGASPFVPMPTEGIKLLNGLYRE FT PTIDNLKKMMNVFVYDASDLTEELFQTRLDNMLSRHEHLDNFVESLAANPRQFPDFGSR FT LAEIQAPTLIVWGRNDRFVPMDAGLRLLAGIPNSSLHVFNNCGHWAQWEHAEPFNRLVL FT DFLQH" FT gene 2912..3709 FT /gene="orcD" FT CDS 2912..3709 FT /codon_start=1 FT /transl_table=11 FT /gene="orcD" FT /product="OrcD" FT /note="hydratase; similar to MhpD" FT /db_xref="GOA:Q400K2" FT /db_xref="InterPro:IPR011234" FT /db_xref="UniProtKB/Swiss-Prot:Q400K2" FT /protein_id="AAQ10536.1" FT /translation="MTISQETLERLAADLRRAEQQGEAIPPLRDAIGAENGEAAYAIQR FT LNVAHALAAGRRLVGRKVGLTNPKVQAQLGVDQPDFGCLFADMAYGDNETVPFDRVLQP FT KIEREIALVLERDLPARTPTWPNVANRHRLGACRPLEIVGTVSPTWNIRFADTVADNAS FT SGLFVLGGPARRLDGLDLRGAAMRMTRNDELVSSGSGAECLGHPLNAAVWLARTLARLG FT DPLKAGDIVLTGALGPMVAVNAGDRFDAEIDGLGRVGVTFSQS" FT gene 3838..>4302 FT /gene="orcF" FT CDS 3838..>4302 FT /codon_start=1 FT /transl_table=11 FT /gene="orcF" FT /product="OrcF" FT /note="acylating dehydrogenase; similar to MhpF" FT /db_xref="GOA:Q400K1" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/TrEMBL:Q400K1" FT /protein_id="AAQ10537.1" FT /translation="MSNKRKVAIIGSGNIGTDLMIKVLRNAQHLEMAAMVGIDPASDGL FT ARASRMGVATTHEGVEGLTRLPIFDEIDFVFDATSAGAHVKNDAFLRGHKPGLRVIDLT FT PAAIGPYCVPVVNLEQHLQALNVNMVTCGGQATIPMVAAVSRVAKVHYAEI" XX SQ Sequence 4302 BP; 705 A; 1542 C; 1369 G; 686 T; 0 other; gatcgtcatc gacctcgcca acgacccgct cggcaccccc aacgtctacc tgtgctgcga 60 cccggtgcgg ccctatgtgt ccgccgcgct gccccacggc gtgcgccgct tcgaattcat 120 ggtgatgccc ggcgagaccg aggaggaact gggcaagccg gagaacctgc gccaactgct 180 ggccaaggtg ctgcccgacc cggacagcgt cgagctgatc cgcaagcgtg tctataccca 240 caacgcgcgc ctggccggac agttccgcgt caagcgcgta ctactagccg gcgatgccgc 300 gcacatcatg ccagtgtggc agggccaggg ttacaacagc ggcatgcgcg acgccggcaa 360 cctggcctgg aaactcgccc tggtggtcaa gggcctggcc ggcgaggcgc tgctggatac 420 ctacgagcag gagcgccgcg cccacgccaa ggcgatgatc gatctctcgg tgctcgccgg 480 ccatgtgctg gcgccaccca agcgctggca gggtgcgctg cgcgacggta tctcctggct 540 gctcaactac ctgccaccgg tgaaacgcta cttcctggaa atgcgtttca agccgatgcc 600 gcagtacgcc aggggtgcgc tggtgtcgga gcagagcggc aaggcctcgc cggtggggcg 660 cctgttcatc cagccgaagg tgctcaccga ggccggcgaa accctgctgc tggacgagtg 720 gatcggcagc aacttcgccg tcatcgcctg gggcaacgac ccgctctggg gcctttcccg 780 cgaacaggtc gaagcctggc gcgcgctggg cacccgcttc atccaggtgc tgccggaggt 840 acagctgaag gccgggcgcg aagtgcccga gggcgtgatc cgcgtcggcg acagcacggg 900 ccgcctgaag gaatggttcg gccgctaccc ggcttccatc gcgctgctgc gcccggaccg 960 cttcgtcgcg ggcgtggcca tcccgcagag cctcggccag gtcggcgacc agttattgcg 1020 cgcgctggct gccatccctc agctcagcag cgccgctggc gtccaggtgg cctgagatga 1080 gcgcctacct gcattgcctg tcgcacaccc cgctggtggg ctacgtcgac ccggtcgccg 1140 aggtgctggc cgaagtggac gaggtggtcg ccgccgcccg cgcgcgcatc gccgccttcg 1200 acccgcagct ggtattcctc ttcgccccgg accactacaa cggcttcttc tatgacgtga 1260 tgccgtcgtt ctgcatcggc atggccgcca cagccatcgg cgacttccac agtctcgccg 1320 ggccgctcga cgtgccccgc gaaaccgccg aggcctgcgc cgcggcggtg ctggaggccg 1380 gcgtggacgc cgcggtgtcc taccgcatgc aggtcgatca tggcttcgcc cagccgctgg 1440 aactgttgct cggcggcctt gcggaaaagc cggtggtccc ggtgttcatc aatggcgtgg 1500 cggtgccgct gccgggcttc cagcgcgccc gcctgctcgg cgaagccatc ggccgtttcg 1560 cccgttccac cggcaagcgc gtcctgttcc tcggttccgg cgggctgtcg caccagccgc 1620 cagtgccgga gctggccaag gtcgacgcac gcatggccga ccgcctgctg ggtagcggcc 1680 gcgacctgcc gaccgacgag cgccaggccc gccagcagcg ggtgatcagc gccgccgaag 1740 gcttcgtcgc cgaccagaac agcctgcacc cgctgaaccc cgagtgggac aaccacttcc 1800 tcgacctgct cgaacagcgc cgtttcgccg agctggacgg cctgggcaac gccgagctgt 1860 cggccctggc cggcaagtcc acccacgagg tgaagacctg ggtgaccgcc ttcgccgcgt 1920 tgtccgcctt tggcccgtac caggcccacg agcgctacta ccggccgatc cccgagtgga 1980 tcgccggctt cggctcgctt tccgcccatt ccctttcctg acctggagac ctgcccatga 2040 ccatcgatat cagcgaagcc agcagcagcc gcttcgcgcg catccgcgaa ggcgagctgg 2100 acctgcagct gcactacaac gacctgggcg agggcgccga gaccgtggtg atgctccatg 2160 gctccggtcc tggcgccagc ggctgggcca acttcagccg caacctggag ccgctgctgg 2220 ccgccggtta ccgggttgtc ctgatggatt gccccggctg gagcaagagc gaccccatcg 2280 tctgccgcag ctcgcgctcg gacctcaacg ccactgcgct caagggcctg ctcgacatgc 2340 tgggcctgga gcgcgtgcac atcctcggca actccatggg cgcccacagt gccgtggcct 2400 tcgccctggc caacccgcgg cgcgtcggca agctggtgct gatgggcggc ggcaccggcg 2460 gcgccagccc cttcgtgccg atgcccaccg agggcatcaa gctgctcaac ggcctgtacc 2520 gcgagccgac catcgacaac ctgaagaaga tgatgaacgt cttcgtctac gacgccagcg 2580 acctcaccga ggaactgttc cagacgcgcc tggacaacat gctcagccgc cacgagcacc 2640 tggacaactt cgtcgaaagc ctggccgcca accccaggca gttccccgac ttcggctcgc 2700 gcctggccga gatccaggcg cccacgctga tcgtctgggg ccgcaacgac cgcttcgtgc 2760 ccatggacgc cggcctgcgc ctgctcgccg gcatcccgaa ctcatcgctg cacgtgttca 2820 acaactgcgg ccactgggcc cagtgggagc acgccgaacc cttcaaccgc ctggtgctgg 2880 actttctcca gcactgaacc cgaggtgccc catgaccatc agccaagaga ccctcgaacg 2940 cctggccgcg gacctgcgcc gcgcggaaca gcagggcgag gccatcccgc ccttgcgtga 3000 cgccatcggc gcggaaaatg gcgaggccgc ctatgccatc cagcgcctca acgtcgccca 3060 cgccctggcc gccggtcgcc gcctggtcgg ccgcaaggtc ggcctgacca accccaaggt 3120 gcaggcccag ctgggcgtgg accagccgga cttcggctgc ctgttcgccg acatggccta 3180 tggcgacaac gaaaccgtgc ccttcgaccg cgtgctgcaa ccgaagatcg aacgggaaat 3240 tgcccttgtg ttggaacgcg acctgccagc gcgaacacca acttggccga acgttgctaa 3300 ccgccaccgg ttgggtgctt gccggccctt ggaaatcgtc ggcaccgtat cgccaacttg 3360 gaacatccgt tttgcggaca ccgtggccga caacgcctcc agcggcttgt tcgtcctcgg 3420 tggcccggcc cgccgcctcg acggcctgga cctacgcggc gcggccatgc gcatgacgcg 3480 taacgacgaa ttggtgtcca gcggcagcgg cgccgaatgc ctgggccacc cgctcaacgc 3540 ggcggtgtgg ctggcacgca ccctggcccg cctaggcgac ccgctgaagg ccggcgacat 3600 cgtccttacc ggcgccctcg ggcccatggt cgcggtgaac gcgggcgacc gtttcgacgc 3660 cgagatcgac ggcctgggcc gcgtcggcgt gaccttcagc cagagctaag agccgaaaaa 3720 agctctggag gctggaaggc tggacgaaaa agcaccgacc tccaccgtgg cagggactgc 3780 ttgtcgttca gcctccagcc ttcagcgctc ttaatcgaat gcttttcgag gtatgccatg 3840 agcaacaaac gcaaagtcgc catcatcggt tccggcaata tcggcaccga tttgatgatc 3900 aaagtcctgc gcaatgcgca gcatctggaa atggccgcca tggtcggcat cgacccggcc 3960 tccgacggtc tggcccgcgc atcacgaatg ggcgtggcca ccacccacga aggcgtggag 4020 ggcctgaccc gcctgccgat cttcgacgag atcgacttcg tcttcgatgc cacttcagcc 4080 ggcgcccacg tgaagaacga cgcgtttctg cggggccaca agcccggtct tcgcgtgatc 4140 gacctgaccc cggccgcaat cggtccgtat tgcgtgccgg tggtcaatct ggagcagcac 4200 ctgcaggcgt tgaacgtgaa tatggtgacc tgtggtgggc aggcgacgat tccgatggtg 4260 gcggcggtgt cccgtgtggc gaaagtccac tacgccgaga tc 4302 // |
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