EBI Dbfetch

ID   AF504062; SV 1; linear; genomic DNA; STD; PRO; 1386 BP.
XX
AC   AF504062;
XX
DT   06-JUN-2002 (Rel. 72, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   Thermococcus sp. GU5L5 alpha-amylase precursor (BD5064) gene, complete cds.
XX
KW   .
XX
OS   Thermococcus sp. GU5L5
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Thermococcus.
XX
RN   [1]
RP   1-1386
RX   DOI; 10.1074/jbc.M203183200
RX   PUBMED; 11994309.
RA   Richardson T.H., Tan X., Frey G., Callen W., Cabell M., Lam D.,
RA   Macomber J., Short J.M., Robertson D.E., Miller C.;
RT   "A novel, high performance enzyme for starch liquefaction. Discovery and
RT   optimization of a low pH, thermostable alpha-amylase";
RL   J. Biol. Chem. 277(29):26501-26507(2002).
XX
RN   [2]
RP   1-1386
RA   Richardson T.H., Tan X., Frey G., Callen W., Cabell M., Lam D.,
RA   Macomber J., Short J.M., Robertson D.E., Miller C.;
RT   ;
RL   Submitted (18-APR-2002) to the EMBL/GenBank/DDBJ databases.
RL   Bioinformatics, Diversa Corporation, 4955 Directors Place, San Diego, CA
RL   92121, USA
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FH   Key             Location/Qualifiers
FH
FT   source          1..1386
FT                   /organism="Thermococcus sp. GU5L5"
FT                   /strain="GU5L5"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:195100"
FT   gene            1..1386
FT                   /gene="BD5064"
FT   CDS             1..1386
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="BD5064"
FT                   /product="alpha-amylase precursor"
FT                   /db_xref="GOA:Q8NKR5"
FT                   /db_xref="HSSP:1MXG"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="UniProtKB/TrEMBL:Q8NKR5"
FT                   /protein_id="AAM48112.1"
FT                   /translation="MKPAKLLVFVLVVSILAGLYAQPAGAAKYLELEEGGVIMQAFYWD
FT                   VPSGGIWWDTIRQKIPEWYDAGISAIWIPPASKGMGGAYSMGYDPYDFFDLGEYDQKGT
FT                   VETRFGSKQELVNMINTAHAYGIKVIADIVINHRAGGDLEWNPFVNDYTWTDFSKVASG
FT                   KYTANYLDFHPNEVKCCDEGTFGGFPDIAHEKSWDQYWLWASNESYAAYLRSIGVDAWR
FT                   FDYVKGYGAWVVKDWLDWWGGWAVGEYWDTNVDALLNWAYSSDAKVFDFPLYYKMDAAF
FT                   DNKNIPALVEALKNGGTVVSRDPFKAVTFVANHDTDIIWNKYPAYAFILTYEGQPTIFY
FT                   RDYEEWLNKDRLKNLIWIHDHLAGGSTDIVYYDNDELIFVRNGYGDKPGLITYINLGSS
FT                   KAGRWVYVPKFAGACIHEYTGNLGGWVDKWVDSSGWVYLEAPAHDPANGYYGYSVWSYC
FT                   GVG"
FT   sig_peptide     1..78
FT                   /gene="BD5064"
XX
SQ   Sequence 1386 BP; 318 A; 408 C; 415 G; 245 T; 0 other;
     atgaagcctg cgaaactcct cgtctttgtg ctcgtagtct ctatcctcgc ggggctctac        60
     gcccagcccg cgggggcggc caagtacctg gagctcgaag agggcggcgt cataatgcag       120
     gcgttctact gggacgtgcc ttcaggagga atatggtggg acacaatacg gcagaagata       180
     ccggagtggt acgatgccgg aatctccgca atatggattc ccccggcgag caagggcatg       240
     ggcggcgcct attcgatggg ctacgacccc tacgacttct ttgacctcgg tgagtacgac       300
     cagaagggaa cggtagagac gcgctttggc tccaagcagg agctcgtgaa catgataaac       360
     accgcccacg cctacggcat caaggtcatc gcagacatag taatcaacca ccgcgccgga       420
     ggagaccttg agtggaaccc cttcgtcaat gactacacct ggacggactt ctcgaaggtc       480
     gcttccggca agtacacggc caactacctc gacttccacc ccaacgaggt caagtgctgc       540
     gacgagggca cctttggagg gttcccggac atagcccacg agaagagctg ggaccagtac       600
     tggctctggg cgagcaacga gagctacgcc gcctacctca ggagcatcgg cgttgacgca       660
     tggcgcttcg actacgtcaa gggctacgga gcgtgggtcg tcaaggactg gctggactgg       720
     tggggaggct gggccgtcgg ggagtactgg gacacaaacg ttgatgcact gctcaactgg       780
     gcctactcga gcgatgcaaa agtcttcgac ttcccgctct actacaagat ggacgcggcc       840
     tttgacaaca agaacattcc cgcactcgtc gaggccctca agaacggggg cacagtcgtc       900
     agccgcgacc cgtttaaggc cgtaaccttc gttgcaaacc acgacacgga cataatttgg       960
     aacaagtacc cggcctacgc cttcatcctc acctacgagg gccagccgac gatattctac      1020
     cgcgactacg aggagtggct caacaaggac aggctcaaga acctcatctg gatacacgac      1080
     cacctcgccg gtggaagcac cgacatagtc tactacgata acgatgaact catcttcgtc      1140
     aggaacggct acggggacaa gccggggctt ataacctaca tcaacctagg ctcgagcaag      1200
     gccgggaggt gggtctacgt tccgaagttc gcgggagcgt gcatccacga gtacaccggc      1260
     aacctcggcg gctgggtgga caagtgggtg gactcaagcg ggtgggtgta cctcgaggcc      1320
     cctgcccacg acccggccaa cggctattac ggctactccg tctggagcta ctgcggggtg      1380
     ggctga                                                                 1386
//