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EBI DbfetchID AF465251; SV 1; linear; genomic DNA; STD; PRO; 4570 BP. XX AC AF465251; XX DT 02-JAN-2003 (Rel. 74, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 4) XX DE Lactobacillus reuteri levansucrase (lev) and putative transposase genes, DE complete cds; and unknown gene. XX KW . XX OS Lactobacillus reuteri OC Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. XX RN [1] RP 1-4570 RX DOI; 10.1099/mic.0.26671-0 RX PUBMED; 14993311. RA van Hijum S.A., Szalowska E., van der Maarel M.J., Dijkhuizen L.; RT "Biochemical and molecular characterization of a levansucrase from RT Lactobacillus reuteri"; RL Microbiology (Reading, Engl.) 150(Pt 3):621-630(2004). XX RN [2] RP 1-4570 RA van Hijum S., Szalowska E., Kralj S., Dondorff M.; RT ; RL Submitted (02-JAN-2002) to the EMBL/GenBank/DDBJ databases. RL Microbiology, University of Groningen, Kerklaan 30, Haren, Gr 9751NN, The RL Netherlands XX FH Key Location/Qualifiers FH FT source 1..4570 FT /organism="Lactobacillus reuteri" FT /strain="121" FT /mol_type="genomic DNA" FT /db_xref="taxon:1598" FT CDS 133..951 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /db_xref="UniProtKB/TrEMBL:Q8GGV5" FT /protein_id="AAO14619.1" FT /translation="MHNGCKLLDGGFYTLTSQERKEAISKDPYADKFIRPYLGAKNFIH FT GTARYCIWLKDANPKDIHQSPFILDRINKVAEFRSQQKSKDTQKYAKRPMLPTRLAYYS FT HDEHTDMLIVPATSSQRREYLPIGYVSEKNIVSYSLMLIPNASNFNFGILESKVHYIWL FT KNFCGRLKSDYRYSNTIIYNNFPWPTVGDKQEQNISETAQGILNTRKLYPDSSLADLYD FT PLTMPVELRKAHEANDKAVLKAYGLSPKATEQEIVEHLFKMYEKLTKGER" FT gene 1193..3607 FT /gene="lev" FT CDS 1193..3607 FT /codon_start=1 FT /transl_table=11 FT /gene="lev" FT /product="levansucrase" FT /EC_number="2.4.1.10" FT /note="sucrose: 2,6-beta-D-fructan 6-beta-D FT fructosyltransferase" FT /db_xref="GOA:Q8GGV4" FT /db_xref="HSSP:1OYG" FT /db_xref="InterPro:IPR019948" FT /db_xref="UniProtKB/TrEMBL:Q8GGV4" FT /protein_id="AAO14618.1" FT /translation="MEYKEHKKMYKVGKNWAVATLVSASILMGGVVTAHADQVESNNYN FT GVAEVNTERQANGQIGVDGKIISANSNTTSGSTNQESSATNNTENAVVNESKNTNNTEN FT AVVNENKNTNNTENAVVNENKNTNNTENDNSQLKLTNNEQPSAATQANLKKLNPQAAKA FT VQNAKIDAGSLTDDQINELNKINFSKSAEKGAKLTFKDLEGIGNAIVKQDPQYAIPYFN FT AKEIKNMPATYTVDAQTGKMAHLDVWDSWPVQDPVTGYVSNYKGYQLVIAMMGIPNSPT FT GDNHIYLLYNKYGDNDFSHWRNAGSIFGTKETNVFQEWSGSAIVNDDGTIQLFFTSNDT FT SDYKLNDQRLATATLNLNVDDNGVSIKSVDNYQVLFEGDGFHYQTYEQFANGKDRENDD FT YCLRDPHVVQLENGDRYLVFEANTGTEDYQSDDQIYNWANYGGDDAFNIKSFFKLLNNK FT KDRELAGLANGALGILKLTNNQSKPKVEEVYSPLVSTLMASDEVERPNVVKLGDKYYLF FT SVTRVSRGSDRELTAKDNTIVGDNVAMIGYVSDSLMGKYKPLNNSGVVLTASVPANWRT FT ATYSYYAVPVAGHPDQVLITSYMSNKDFASGEGNYATWAPSFLVQINPDDTTTVLARAT FT NQGDWVWDDSSRNDNMLGVLKEGAANSAALPGEWGKPVDWSLINRSSGLGLKPHQPVQP FT KIDQPDQQPSGQNTKNVTPGNGDKPAGKATPDNTNIDPSAQPSGQNTNIDPSAQPSGQN FT TKNVTPGNEKQGKNTDAKQLPQTGNKSGLAGLYAGSLLALFGLAAIEKRHA" FT repeat_region 1444..1563 FT /rpt_type=DIRECT FT /rpt_unit_range=1444..1479 FT repeat_region 1480..1579 FT /rpt_type=DIRECT FT /rpt_unit_range=1480..1537 FT repeat_region 3295..3477 FT /rpt_type=DIRECT FT /rpt_unit_range=3295..3339 FT stem_loop 3675..3721 FT CDS complement(3895..4299) FT /codon_start=1 FT /transl_table=11 FT /product="putative transposase" FT /db_xref="GOA:Q8GGV3" FT /db_xref="InterPro:IPR001584" FT /db_xref="UniProtKB/TrEMBL:Q8GGV3" FT /protein_id="AAO14620.1" FT /translation="MMPTTPQQRHIRLAIKHGNEVIEDFKQHSTGKLNLWVSITDLSQK FT YNITVEAQGRINPGIITAIVGQLPFSEFKYQGLIDLLGSIPKEFVHSLTPDHGREFLGL FT NDIRGRLGVAIYWPDPYSPEERGPNENTNG" XX SQ Sequence 4570 BP; 1524 A; 818 C; 884 G; 1344 T; 0 other; gttaacaaag acaaaatttt atataattct tcaaattaaa tttcccactg taagaacata 60 aatgggtacc tgtttgatgg gaataatata tttgtaacta accggccggc acctctttct 120 aatgtgccta ggatgcataa tggatgtaaa ttactagatg gcggttttta tacattaacc 180 tcgcaggaga gaaaagaagc aattagtaag gatccatatg cagataaatt tattaggcct 240 tatttaggtg ctaaaaattt cattcatgga actgctaggt actgtatttg gttaaaggac 300 gcaaacccga aagatatcca tcaatcgcca tttatactgg atagaatcaa taaagtagcg 360 gaattcagat cgcagcaaaa aagtaaagat acacaaaaat atgcaaaacg gcccatgcta 420 ccaacacgac ttgcctatta tagccacgat gaacatacgg atatgctgat agtacctgca 480 acatcatcgc aacgtagaga atatcttcca attggatatg tttcagaaaa gaatattgtg 540 tcttattcac taatgctaat ccccaatgct agtaatttta atttcggtat tctagaatct 600 aaagttcact atatttggtt aaaaaacttt tgcggtcggt tgaagtccga ttatcgttat 660 tcaaacacta ttatttataa taatttccct tggccgactg ttggtgacaa gcaagaacaa 720 aacatttctg aaactgctca aggtatatta aatactcgca agctctatcc agacagctca 780 ctggctgatc tttatgatcc actaacaatg ccagttgaac ttcgtaaagc tcatgaagcc 840 aatgataaag ctgttcttaa agcatatgga ttgagtccta aagctactga gcaagaaatc 900 gtagaacatc tatttaagat gtatgaaaaa ctgactaaag gtgaaagata actttgtaaa 960 accaatattt tataaagaca gtaaatgtta atttgataaa aacatatatt taataaacaa 1020 aagtgatata atcaagtagt tctttgtatt acaaaataca tttaatatct ctcagcattt 1080 tgcatactgg gagatttttt attgacaaat tgtttgaaag tgcttatgat gaaaccgtgt 1140 agaaactaat tcaatttgat aaacgttaga catttctgag gaggaagtca ttttggagta 1200 caaagaacat aagaaaatgt ataaagtcgg caagaattgg gccgttgcta cattggtatc 1260 agcttcaatt ttaatgggag gggttgtaac cgctcatgct gatcaagtag aaagtaacaa 1320 ttacaacggt gttgctgaag ttaatactga acgtcaagct aatggtcaaa ttggcgttga 1380 tggaaaaatt attagtgcta acagtaatac aaccagtggc tcgacaaatc aagaatcatc 1440 tgctactaac aatactgaaa atgctgttgt taatgaaagc aaaaatacta acaatactga 1500 aaatgctgtt gttaatgaaa acaaaaatac taacaatact gaaaatgctg ttgttaatga 1560 aaacaaaaat actaacaaca cagaaaacga taatagtcaa ttaaagttaa ctaataatga 1620 acaaccatca gccgctactc aagcaaactt gaagaagcta aatcctcaag ctgctaaggc 1680 tgttcaaaat gccaagattg atgccggtag tttaacagat gatcaaatta atgaattaaa 1740 taagattaac ttctctaagt ctgctgaaaa gggtgcaaaa ttgaccttta aggacttaga 1800 ggggattggt aatgctattg ttaagcaaga tccacaatat gctattcctt attttaatgc 1860 taaggaaatc aagaatatgc ctgcgacata cactgtagat gcccaaacag gtaagatggc 1920 tcatcttgat gtctgggact cttggccagt acaagatcct gtcacaggtt atgtatctaa 1980 ttacaagggt tatcaactag ttattgctat gatgggtatt ccaaattcgc caactggaga 2040 taatcatatc tatcttcttt acaacaagta tggtgataat gacttttctc attggcgcaa 2100 tgcaggttca atctttggaa ctaaagaaac aaatgtgttc caagaatggt caggttcagc 2160 tattgtaaat gatgatggta caattcaact atttttcacc tcaaatgata cgtctgatta 2220 caagttgaat gatcaacgcc ttgctaccgc aacattaaac cttaatgttg atgataacgg 2280 tgtttcaatc aagagtgttg ataattatca agttttgttt gaaggtgatg gatttcacta 2340 ccaaacttat gaacaattcg caaacggcaa agatcgtgaa aatgatgatt actgcttacg 2400 tgacccacac gttgttcaat tagaaaatgg tgatcgttat cttgtattcg aagctaatac 2460 tgggacagaa gattaccaaa gtgacgacca aatttataat tgggctaact atggtggcga 2520 tgatgccttc aatattaaga gtttcttcaa gcttttgaat aataagaagg atcgtgaatt 2580 ggctggttta gctaatggtg cacttggtat cttaaagctc actaacaatc aaagtaagcc 2640 aaaggttgaa gaagtatact caccattggt atctactttg atggctagtg atgaagttga 2700 acgaccaaac gtagttaagc ttggtgataa gtattatctc ttctccgtaa ctcgtgtaag 2760 tcgtggttcc gatcgtgaat taaccgctaa ggataacaca atcgttggtg ataacgttgc 2820 tatgattggt tacgtttccg atagcttaat gggtaagtac aagccattaa ataactcagg 2880 tgtcgtatta actgcatcag tacctgcaaa ctggcgtact gctacttatt cctactatgc 2940 agtacctgta gctggtcatc ctgatcaagt attaattact tcttacatga gtaacaagga 3000 ctttgcttca ggtgaaggaa actatgcaac ttgggcacca agtttcttag tacaaatcaa 3060 tccagatgac acgacaactg tattagcacg tgcaactaac caaggtgact gggtgtggga 3120 cgactctagt cggaacgata atatgctcgg tgttcttaaa gaaggtgcag ctaacagtgc 3180 cgccttacca ggtgaatggg gtaagccagt tgactggagt ttgattaaca gaagttctgg 3240 cttaggctta aagcctcatc aaccagttca accaaagatt gatcaacctg atcaacaacc 3300 ttctggtcaa aacactaaga atgtcacacc aggtaatggt gataagcctg ctggtaaggc 3360 aactcctgat aacactaata ttgatccaag tgcacaacct tctggtcaaa acactaatat 3420 tgatccaagt gcacaacctt ctggtcaaaa cactaagaat gtcacaccag gtaatgagaa 3480 acaaggtaag aataccgatg caaaacaatt accacaaaca ggtaataagt ctggtttagc 3540 aggactttac gctggttcat tacttgcctt gtttggattg gcagcaattg aaaagcgtca 3600 cgcttaatag agtaaaaaaa catcctcact caagttacaa agtagaataa tatgtattat 3660 ttctacgctt agtcaagagg aattactgac attatttgct ttcatgtcgg tagttcctct 3720 tttcttttgt gacgaattgt caaaccaagt gcaacggttt ctcaaaaaac acctcatatg 3780 gggtttcata atttaacact tttcgaggac ggcggttcag ctgatgttgg cagaaactga 3840 cgtccttatc tgtataatca tcaatattag tccttttagg aaagtattcc ctaattagcc 3900 attggtattt tcattgggtc ctctttcctc tggtgaatag ggatctggcc aatagatagc 3960 tactcctaaa cgtcctcgaa tatcattcaa gccaagaaat tcacgcccat gatctggagt 4020 caatgaatgg acaaattctt taggaataga ccctaagaga tcaattaagc cctgatattt 4080 gaattcggag aaggggagtt gtccaacaat tgccgttata ataccagggt taatacggcc 4140 ctgggcctct acggtaatat tgtatttttg gctcagatca gtgatagaaa cccacagatt 4200 tagcttgccg gtggagtgct gcttgaagtc ttcaattact tcgttaccat gtttgattgc 4260 taatctgatg tgtcgttgtt gtggtgtagt aggcatcata ccacctcctc ataaaataag 4320 gtataacagg aatttcttgt actatatgat ccttccaata taataatatt aggccgataa 4380 gaaatgacca gctaccattt cttgatgctt agtgaatata atcggatgat acgtcacccc 4440 tcaacaatcc aatttcacgg aggtgagtaa tcatgccgag agctaggaat gattggagga 4500 acgaacacgg tccatgcggc agtggctatt tggattttag ccaaagcagc gttactgctt 4560 gcaaaagctt 4570 // |
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