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EBI DbfetchID AF459437; SV 1; linear; genomic DNA; STD; PRO; 3988 BP. XX AC AF459437; XX DT 10-SEP-2002 (Rel. 73, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Lactobacillus reuteri inulosucrase (inu) gene; and unknown gene. XX KW . XX OS Lactobacillus reuteri OC Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. XX RN [1] RP 1-3988 RX DOI; 10.1128/AEM.68.9.4390-4398.2002 RX PUBMED; 12200292. RA Van Hijum S.A.F.T., Van Geel-Schutten G.H., Rahaoui H., RA Van Der Maarel M.J.E.C., Dijkhuizen L.; RT "Characterization of a novel fructosyltransferase from Lactobacillus RT reuteri that synthesizes high-molecular-weight inulin and inulin RT oligosaccharides."; RL Appl. Environ. Microbiol. 68(9):4390-4398(2002). XX RN [2] RP 1-3988 RA van Hijum S., Rahaoui H.; RT ; RL Submitted (13-DEC-2001) to the EMBL/GenBank/DDBJ databases. RL Microbiology, Rijks Universiteit Groningen, Kerklaan 30, Haren, GR 9751NN, RL Netherlands XX FH Key Location/Qualifiers FH FT source 1..3988 FT /organism="Lactobacillus reuteri" FT /strain="121" FT /mol_type="genomic DNA" FT /db_xref="taxon:1598" FT CDS complement(132..986) FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="ORF2" FT /db_xref="GOA:Q8GP33" FT /db_xref="InterPro:IPR000463" FT /db_xref="UniProtKB/TrEMBL:Q8GP33" FT /protein_id="AAN05574.1" FT /translation="MLYNAALVLEGGAFRGQYTAGIVDTFLAHHIEFRSVIGVSAGSLC FT GVNFVSKQYGRAANININHRHDRQYISMARVFKKQIINLDYLFEDHGYSWQNFNEAAYR FT RSASHFTAVATSVKTGKTVLFTDPVGEELTNALKASSSMPFLSDPQETSQGPCLDGGIT FT DSIPFDIAQQQGYDKIVVVRTRDVNYRKKPSSSAVKKLYDMVYKDYPEFAKAGINRPLL FT YNQQIAEINRLSREGKIFNIAPSKPIKIKRIEGNIKKIRALYETGRKEGEEIVPALVDY FT LTN" FT CDS 1432..3828 FT /codon_start=1 FT /transl_table=11 FT /gene="inu" FT /product="inulosucrase" FT /EC_number="2.4.1.9" FT /note="Inu; sucrose: 2,1-beta-D-fructan 1-beta-D FT fructosyl-transferase" FT /db_xref="GOA:Q8GP32" FT /db_xref="HSSP:1OYG" FT /db_xref="InterPro:IPR019948" FT /db_xref="UniProtKB/TrEMBL:Q8GP32" FT /protein_id="AAN05575.1" FT /translation="MLERKEHKKMYKSGKNWAVVTLSTAALVFGATTVNASADTNIENN FT DSSTVQVTTGDNDIAVKSVTLGSGQVSAASDTTIRTSANANSASSAANTQNSNSQVASS FT AAITSSTSSAASSNNTDSKAAQENTNTAKNDDTQKAAPANESSEAKNEPAVNVNDSSAA FT KNDDQQSSKKNTTAKLNKDAENVVKKAGIDPNSLTDDQIKALNKMNFSKAAKSGTQMTY FT NDFQKIADTLIKQDGRYTVPFFKASEIKNMPAATTKDAQTNTIEPLDVWDSWPVQDVRT FT GQVANWNGYQLVIAMMGIPNQNDNHIYLLYNKYGDNELSHWKNVGPIFGYNSTAVSQEW FT SGSAVLNSDNSIQLFYTRVDTSDNNTNHQKIASATLYLTDNNGNVSLAQVANDHIVFEG FT DGYYYQTYDQWKATNKGADNIAMRDAHVIEDDNGDRYLVFEASTGLENYQGEDQIYNWL FT NYGGDDAFNIKSLFRILSNDDIKSRATWANAAIGILKLNKDEKNPKVAELYSPLISAPM FT VSDEIERPNVVKLGNKYYLFAATRLNRGSNDDAWMNANYAVGDNVAMVGYVADSLTGSY FT KPLNDSGVVLTASVPANWRTATYSYYAVPVAGKDDQVLVTSYMTNRNGVAGKGMDSTWA FT PSFLLQINPDNTTTVLAKMTNQGDWIWDDSSENLDMIGDLDSAALPGERDKPVDWDLIG FT YGLKPHDPATPNDPETPTTPETPETPNTPKTPKTPENPGTPQTPNTPNTPEIPLTPETP FT KQPETQTNNRLPQTGNNANKAMIGLGMGTLLSMFGLAEINKRRFN" XX SQ Sequence 3988 BP; 1360 A; 715 C; 733 G; 1180 T; 0 other; tgatcatatt cttcaagagt taaaaattta acattctcta cccatatttt ccgtttcccc 60 ttaatttgac cgttagtttt atttttggga atataaacgc gttttttagt tgacataata 120 tttctccttt attaatttgt gaggtaatca actaaagcag ggacgatttc ctctccttct 180 ttccgcccag tttcataaag tgctcgaatc ttcttaatat tcccttcaat ccgcttaatt 240 ttaattggtt tagagggagc aatattaaag atctttcctt cacgagataa acgattgatt 300 tcagcaatct gttggttgta gagaagggga cgattaatcc ccgctttagc aaattcggga 360 taatctttat ataccatgtc gtataacttc tttacagcag aactggaggg tttctttcgg 420 tagttaacat ccctagtccg gacaacaaca atcttgtcat atccttgttg ttgtgcaata 480 tcaaatggaa ttgaatctgt gatcccacca tcaaggcaag gtccctggga tgtttcctgt 540 ggatctgaaa gaaatggcat tgatgaggaa gctttgaggg cattggttaa ctcctcaccg 600 acaggatccg taaataaaac tgtttttcct gtttttactg aagtagcgac cgctgtgaaa 660 tgagatgctg accgtcgata ggctgcttca ttaaaattct gccaagaata tccgtgatcc 720 tcaaaaaggt aatcaagatt aattatttgc tttttaaata cacgagccat cgagatatat 780 tggcgatcgt gccgatgatt aatattgata ttagcagcgc gtccatattg cttagagacg 840 aaattaactc cacaaagcga accagcagaa acgccaatta cacttcgaaa ttcaatatgg 900 tgagcgagaa atgtatcaac aattccggca gtgtattggc cacggaatgc gccaccttct 960 aaaactaaag ctgcattata taacataaaa tgaaataact cctttttgtc cgccaaaaat 1020 agcaaaaatg acctaatttt tagtgagaca ctttttcaat aaaattatac aacagtctta 1080 cacgaaaata taggatttta aaagcttgtt atatcaacgt ttattctcgg ttaatgtaag 1140 actcttttat caaaagagtc ttactaattt taaaataatg caaaaatagg ggtaaaaaca 1200 gcttttgcct ctcagacgaa ctgaaattca ttttgatgag tagtaggtcg ttacaattcg 1260 tctcagaaga aaaatgcata ttactatcaa aaaatgagta aatagacagt aaatatttaa 1320 atatgaactt tttatgaaga aactattgat aatttaagat aatcataaga aaatagtgca 1380 cagttaattg ttaaacgttt agcaaaaagg taaagaaacg gggttacttc tatgctagaa 1440 cgcaaggaac ataaaaaaat gtataaaagc ggtaaaaatt gggcagtcgt tacactctcg 1500 actgctgcgc tggtatttgg tgcaacaact gtaaatgcat ccgcggacac aaatattgaa 1560 aacaatgatt cttctactgt acaagttaca acaggtgata atgatattgc tgttaaaagt 1620 gtgacacttg gtagtggtca agttagtgca gctagtgata cgactattag aacttctgct 1680 aatgcaaata gtgcttcttc tgccgctaat acacaaaatt ctaacagtca agtagcaagt 1740 tctgctgcaa taacatcatc tacaagttcc gcagcttcat caaataacac agatagtaaa 1800 gcggctcaag aaaatactaa tacagccaaa aatgatgaca cgcaaaaagc tgcaccagct 1860 aacgaatctt ctgaagctaa aaatgaacca gctgtaaacg ttaatgattc ttcagctgca 1920 aaaaatgatg atcaacaatc cagtaaaaag aatactaccg ctaagttaaa caaggatgct 1980 gaaaacgttg taaaaaaggc gggaattgat cctaacagtt taactgatga ccagattaaa 2040 gcattaaata agatgaactt ctcgaaagct gcaaagtctg gtacacaaat gacttataat 2100 gatttccaaa agattgctga tacgttaatc aaacaagatg gtcggtacac agttccattc 2160 tttaaagcaa gtgaaatcaa aaatatgcct gccgctacaa ctaaagatgc acaaactaat 2220 actattgaac ctttagatgt atgggattca tggccagttc aagatgttcg gacaggacaa 2280 gttgctaatt ggaatggcta tcaacttgtc atcgcaatga tgggaattcc aaaccaaaat 2340 gataatcata tctatctctt atataataag tatggtgata atgaattaag tcattggaag 2400 aatgtaggtc caatttttgg ctataattct accgcggttt cacaagaatg gtcaggatca 2460 gctgttttga acagtgataa ctctatccaa ttattttata caagggtaga cacgtctgat 2520 aacaatacca atcatcaaaa aattgctagc gctactcttt atttaactga taataatgga 2580 aatgtatcac tcgctcaggt agcaaatgat catattgtat ttgaaggtga tggctattac 2640 taccaaactt atgatcaatg gaaagctact aacaaaggtg ccgataatat tgcaatgcgt 2700 gatgctcatg taattgaaga tgataatggt gatcggtacc ttgtttttga agcaagtact 2760 ggtttagaaa attatcaagg cgaggaccaa atttataact ggttaaatta tggcggagat 2820 gacgcattta atatcaagag cttatttaga attctttcca atgatgatat taagagtcgg 2880 gcaacttggg ctaatgcagc tatcggtatc ctcaaactaa ataaggacga aaagaatcct 2940 aaggtggcag agttatactc accattaatt tctgcaccaa tggtaagcga tgaaattgag 3000 cgaccaaatg tagttaaatt gggtaataaa tattacttat ttgccgctac ccgtttaaat 3060 cgaggaagta atgatgatgc ttggatgaat gctaattatg ccgttggtga taatgttgca 3120 atggtcggat atgttgctga tagtctaact ggatcttata agccattaaa tgattctgga 3180 gtagtcttga ctgcttctgt tcctgcaaac tggcgaacag caacttattc atattatgct 3240 gtccccgttg ccggaaaaga tgaccaagta ttagttactt catatatgac taatagaaat 3300 ggagtagcgg gtaaaggaat ggattcaact tgggcaccga gtttcttact acaaattaac 3360 ccggataaca caactactgt tttagctaaa atgactaatc aaggggattg gatttgggat 3420 gattcaagcg aaaatcttga tatgattggt gatttagact ccgctgcttt acctggcgaa 3480 cgtgataaac ctgttgattg ggacttaatt ggttatggat taaaaccgca tgatcctgct 3540 acaccaaatg atcctgaaac gccaactaca ccagaaaccc ctgagacacc taatactccc 3600 aaaacaccaa agactcctga aaatcctggg acacctcaaa ctcctaatac acctaatact 3660 ccggaaattc ctttaactcc agaaacgcct aagcaacctg aaacccaaac taataatcgt 3720 ttgccacaaa ctggaaataa tgccaataaa gccatgattg gcctaggtat gggaacattg 3780 cttagtatgt ttggtcttgc agaaattaac aaacgtcgat ttaactaaat actttaaaat 3840 aaaaccgcta agccttaaat tcagcttaac ggttttttat tttaaaagtt tttattgtaa 3900 aaaagcgaat tatcattaat actaatgcaa ttgttgtaag accttacgac agtagtaaca 3960 atgaatttgc ccatctttgt cggctagc 3988 // |
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