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EBI DbfetchID AF456132; SV 1; linear; genomic DNA; STD; PRO; 2761 BP. XX AC AF456132; XX DT 24-JAN-2002 (Rel. 70, Created) DT 18-DEC-2003 (Rel. 78, Last updated, Version 2) XX DE Bacillus subtilis isolate RO-A-4 quorum-sensing gene locus, partial DE sequence. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2761 RA Ansaldi M., Marolt D., Stebe T., Mandic-Mulec I., Dubnau D.; RT "Isoprenylated peptide pheromones specifically activate the quorum-sensing RT systems that govern genetic competence in Bacillus subtilis"; RL Unpublished. XX RN [2] RP 1-2761 RA Ansaldi M., Marolt D., Stebe T., Mandic-Mulec I., Dubnau D.; RT ; RL Submitted (07-DEC-2001) to the EMBL/GenBank/DDBJ databases. RL Public Health Research Institute, 455 First Avenue, New York, NY 10016, USA XX FH Key Location/Qualifiers FH FT source 1..2761 FT /organism="Bacillus subtilis" FT /isolate="RO-A-4" FT /mol_type="genomic DNA" FT /note="group: 168" FT /db_xref="taxon:1423" FT gene 1..840 FT /gene="comQ" FT CDS 1..840 FT /codon_start=1 FT /transl_table=11 FT /gene="comQ" FT /product="pre-ComX modifying enzyme" FT /note="ComQ" FT /db_xref="GOA:Q8VQF0" FT /db_xref="InterPro:IPR008949" FT /db_xref="InterPro:IPR017446" FT /db_xref="UniProtKB/TrEMBL:Q8VQF0" FT /protein_id="AAL67721.1" FT /translation="MKEIVHGKIQNLDLKEYLINFIDEKNHFSFGILSFKHYVALSGNS FT SSHILTLAGGIELLILAFDIFDDLEDEDNIEIKWMKIDPSLALNAATTLYTLGLETICS FT ISNSAEFHRLTFKYALNAMQGQHEDLRNSPETEEECIQMMKQKAGSLTAMSAVLAAMLA FT NGEFNQTIEDYAYKIGIIKQLENDYYGLVNDQRSDIRKKRKTLIYLFLNRKFNEASEKI FT LKLINSHTSYHSFISDSSKFDELLFEAGLNQYVSMLIKLYEEEITTSMNQLNINIKL" FT gene 855..1031 FT /gene="comX" FT CDS 855..1031 FT /codon_start=1 FT /transl_table=11 FT /gene="comX" FT /product="ComX pheromone precursor" FT /note="pre-ComX" FT /db_xref="GOA:Q8VL79" FT /db_xref="InterPro:IPR009233" FT /db_xref="UniProtKB/Swiss-Prot:Q8VL79" FT /protein_id="AAL67722.1" FT /translation="MKQDMIDYLMKNPQVLTKLENGEASLIGIPDKLIPSIVDIFNKKM FT TLSKKCKGIFWEQ" FT gene 1051..>2761 FT /gene="comP" FT CDS 1051..>2761 FT /codon_start=1 FT /transl_table=11 FT /gene="comP" FT /product="histidine kinase sensor protein" FT /note="ComP" FT /db_xref="GOA:Q8VQE9" FT /db_xref="UniProtKB/TrEMBL:Q8VQE9" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAL67723.1" FT /translation="MKNLKRNFSIVLVLFSLLYFAYIVYFTQNGILVGTYVSETANHEL FT KVEKMTKVTYGKYMGLKEGDLILEVSGKKPTSENVKRDYLAKAEHLKVKRDNHEIQLHN FT TEYISLNNSYSFFIYIIPCIFFALSLLCAFYIYRVNRVKNTYSAYILINFLLFISIAYL FT SAGGVTRGYEINRYINLFTFLNVPVLYLHFLYQYFKELSIKFSSRYIVNILYTLPIINV FT IIEFIQKYLPSGIITNISNINLVTFLIEITVVYFCIIYYGIIYRKKDHYYLLKVLLISN FT TVAFFPFTVLYIMPEVLFGQYIYSAIVTAPFLIIIPLALVYQFVVNKIYDIDFILGRIR FT YYSLLSIIPTIGIVWLSIIFDSKRADFNIILITLCTFIIFLSVFYIKELLDYKFRLKRF FT SEKYNYQDSIFKYTQLIKGVSALDQVFKNLKTTILEVLPVSKAYIFEVGADREIIYFDK FT QSVEPNWRLYKTEFEKVTKEIGKIIEINQGFLIKVGEKGTSSYVLLWLSSINTPRLTRD FT EISWLKTLSFYTSVSMENVVQIEELMDHLQALKREGANPVWLKKLMYTIEEKQ" FT misc_feature 1118 FT /gene="comP" FT /note="alternate site of translation initiation" XX SQ Sequence 2761 BP; 988 A; 357 C; 467 G; 949 T; 0 other; atgaaggaga ttgtacacgg aaaaattcag aatttggatt tgaaagagta tttaatcaac 60 tttatcgatg agaaaaatca cttttctttt ggtatccttt cttttaagca ttacgtagca 120 ttaagcggta atagttcttc gcatatttta acattggcag gaggaataga acttttaatc 180 ttagccttcg atatatttga tgatttagag gatgaggaca acattgaaat aaaatggatg 240 aaaattgatc cttctctcgc tctaaatgct gcaactaccc tttatacttt agggttagaa 300 actatttgtt ctattagcaa tagcgctgaa tttcatcggc taactttcaa atatgcatta 360 aatgctatgc agggacaaca tgaggattta aggaactctc ctgagacgga agaagaatgt 420 attcaaatga tgaaacaaaa agcaggttct cttacagcta tgtctgccgt tttagctgct 480 atgcttgcga atggagagtt taatcaaact attgaggatt atgcttataa gatagggata 540 ataaaacaat tggaaaatga ctattatggt ttagttaatg atcaacgtag cgatataaga 600 aaaaaaagaa agactttgat ttatttattt ttaaaccgca aatttaatga ggcttctgaa 660 aagatattaa aattaataaa ttctcatact agttatcata gttttatatc tgatagctct 720 aaatttgatg aattattatt tgaagcgggg cttaatcaat atgtatctat gttgattaag 780 ctttatgagg aagaaatcac aacaagcatg aatcaattaa atataaatat aaagttataa 840 agaggggtat acttatgaaa caagacatga ttgattactt aatgaagaat cctcaagttt 900 taactaaatt agaaaatgga gaggcgagct taataggaat tccagataaa ctaatccctt 960 caattgtaga tattttcaac aaaaaaatga ctctctcaaa gaaatgcaag ggaattttct 1020 gggagcaata attcagagag aagtggtcta ttgaaaaacc ttaaaagaaa tttttctata 1080 gtgctagtat tatttagtct cttatatttt gcttatattg tttatttcac tcagaatggt 1140 atcttagttg gtacttatgt ttctgaaaca gccaatcatg agttaaaagt tgaaaaaatg 1200 actaaagtca catatggaaa atatatgggc ttaaaagaag gcgacttaat actcgaggta 1260 agtgggaaga aacctacaag cgagaatgtt aaaagagatt atttagcaaa agctgaacat 1320 ttaaaagtta aaagggataa tcatgaaatt caattacata atacggagta tatctcttta 1380 aataattctt atagcttttt catttatatt atcccttgta tattttttgc tttgagtctg 1440 ttatgtgcat tttatattta tagggtcaat cgagtaaaaa atacgtactc ggcttatatt 1500 ttgattaatt ttttgctgtt tatatctata gcatatttaa gtgctggagg cgtaactaga 1560 ggatatgaaa taaatagata tattaatctt tttacctttt taaatgtacc tgtcttgtat 1620 ttgcattttc tatatcaata tttcaaagaa ttaagcatca aattttcatc acgttatatc 1680 gtgaatattt tatacacgtt gccaattatc aatgtaataa tagagttcat ccagaaatac 1740 cttccttcgg ggattattac gaatattagt aatatcaatt tagtaacatt cttaatagag 1800 attacagttg tttatttttg cattatctat tatgggataa tttatagaaa aaaggatcat 1860 tattatttgt tgaaggtatt attgatttca aatacggtag ctttctttcc ttttacggtc 1920 ttatatatca tgccggaagt tttatttggg caatatattt actcagcaat agtgactgcg 1980 ccattcttaa taattatccc attagcattg gtttatcaat tcgttgtaaa taaaatttat 2040 gatatagact ttatactagg cagaattaga tattatagtt tattatccat cataccaaca 2100 ataggcatag tatggttgtc aataatattt gatagcaaaa gagcggattt taatatcatc 2160 ttaattacgt tatgtacttt cataattttc ctgtcggtct tttatattaa agaattactg 2220 gactataaat ttcggttaaa acgattttcg gaaaaatata attatcaaga cagtatattt 2280 aaatatacgc aattaattaa aggtgtatca gctttagatc aagtatttaa gaacttaaaa 2340 acaacaatac tagaagttct acccgtaagt aaagcctaca tctttgaagt tggtgctgat 2400 agagaaatca tatactttga taaacagagt gttgaaccca actggaggct gtataaaaca 2460 gaatttgaaa aggtcactaa agaaataggg aagattattg agattaacca aggctttttg 2520 atcaaagtag gggaaaaagg tacgagttct tatgtattgc tttggttatc tagcatcaat 2580 acaccaaggt tgacaagaga tgaaatttca tggctgaaaa cattatcgtt ttatactagt 2640 gtatccatgg aaaatgttgt acaaattgaa gaactcatgg atcatttaca agctttaaaa 2700 agggaaggtg ccaaccccgt atggctcaaa aaactcatgt acacgatcga ggaaaaacaa 2760 c 2761 // |
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