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EBI DbfetchID AF449413; SV 1; linear; mRNA; STD; FUN; 2666 BP. XX AC AF449413; XX DT 04-DEC-2002 (Rel. 74, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Piromyces equi cellulase Cel48A precursor (cel48A) mRNA, complete cds. XX KW . XX OS Piromyces equi OC Eukaryota; Fungi; Neocallimastigomycota; Neocallimastigomycetes; OC Neocallimastigales; Neocallimastigaceae; Piromyces. XX RN [1] RP 1-2666 RX DOI; 10.1080/1042517021000024191 RX PUBMED; 12652902. RA Steenbakkers P.J.M., Freelove A., van Cranenbroek B., Sweegers B.M.C., RA Harhangi H.R., Vogels G.D., Hazlewood G.P., Gilbert H.J., RA Op den Camp H.J.M.; RT "The major component of the cellulosomes of anaerobic fungi from the genus RT Piromyces is a family 48 glycoside hydrolase"; RL DNA Seq. 13(6):313-320(2002). XX RN [2] RP 1-2666 RA Freelove A., Steenbakkers P.J.; RT ; RL Submitted (19-NOV-2001) to the EMBL/GenBank/DDBJ databases. RL Microbiology, University of Nijmegen, Toernooiveld 1, Nijmegen NL-6525 ED, RL The Netherlands XX FH Key Location/Qualifiers FH FT source 1..2666 FT /organism="Piromyces equi" FT /mol_type="mRNA" FT /db_xref="taxon:99929" FT repeat_region 48..68 FT /note="palindrome" FT CDS 107..2389 FT /codon_start=1 FT /gene="cel48A" FT /product="cellulase Cel48A precursor" FT /note="glycosyl hydrolase family 48; corresponds to the FT cellulosome major component" FT /db_xref="GOA:Q8J1E2" FT /db_xref="HSSP:1E8P" FT /db_xref="InterPro:IPR000556" FT /db_xref="UniProtKB/TrEMBL:Q8J1E2" FT /protein_id="AAN76735.1" FT /translation="MPSIRSSLALLGATAAFAAPAMRKRYNDEYAQRVTDLYDTMTGNG FT SYSSEYFSPEKVPYHSVETLMVEAPDQGHESVSETYSFWIWLEAVNGKITGNYDGVEEA FT WSYLEKHIIPDSKNQPGNSRYNPSSPATYAAEHDEIYDYPSKLIFQDGLVGEDPIAKEL FT QQAYGNWDIYIMHWIIDGDNWYGYGQQGDGTSKPSFINTFQRGPSESTWKTVPHPCWEA FT MKWGGRNGFLDLFTVDNSYAKQWRYTAAPDADARAIQAAYFAYMWAEEDGVNLSSVASK FT AAKLGDYLRYAQYDKYFKKIGNCVGYDKCSAGRGKNSAHYLISWYFAWGGGLQGDWAWR FT IGSSHTHTGYQNPLAAWILSTQSAFKPKSSTGAKDWATSLDRQLELFRWLQSAEGCIAG FT GATNSWQGAYEQPSSDITTFYGMWYDWQPVYHDPPSNNWTGMQGWGMERVCSLYYLSGN FT EKAGKVCQEWAKWVKNTTRVTGEEIVHATTLDWEGNPDEWNASNFNKSNLNRSLHGTVS FT SEGVDLGTIASIMKGLMWVSMKDNDQEGINLAVQVMDAIEGYRDNLGYSSLEARGDYEK FT FGGEVYIPSGWTGKNAQGANLKNGVTFIDIRPKYKQDPDWPQVEEFLNGGNPPEFNYHR FT FWAQTEIAVANGLISIYGLKSTGGSSPIYGGDEVTECPASITRQGYSCCKVGCQVVYQD FT ADGDWGVENNDWCGCGKAPAPKPKCPTSITNQGYSCCSSCGPVYYQDADGDWGVENGDW FT CGMPTSC" FT sig_peptide 107..181 FT /gene="cel48A" FT mat_peptide 182..2386 FT /gene="cel48A" FT /product="cellulase Cel48A" FT /note="N-terminal 25 amino acids of extracellular protein FT determined by Edman degradation" FT misc_feature 182..1957 FT /gene="cel48A" FT /note="Region: glycosyl hydrolase family 48 catalytic FT domain" FT misc_feature 2012..2131 FT /gene="cel48A" FT /note="Region: fungal dockerin" FT misc_feature 2153..2386 FT /gene="cel48A" FT /note="Region: fungal dockerin" XX SQ Sequence 2666 BP; 816 A; 531 C; 544 G; 775 T; 0 other; ggcacgagaa ttaattgatt tttagttcgc cggaacgaac ttaatcttat tattattgaa 60 taataatatt agtttaaact tttaaaagtt tttttttttt tgaaaaatgc catctattag 120 atcttctctt gctcttctcg gtgctactgc cgcttttgct gctccagcta tgagaaagcg 180 ttacaacgat gaatacgctc aacgtgttac cgatctttac gataccatga ctggtaacgg 240 ttcttacagt tctgaatact tctcaccaga aaaggttcca taccactctg ttgaaactct 300 tatggttgaa gctccagatc aaggtcacga atctgtctca gaaacttact ctttctggat 360 ttggttagaa gccgttaacg gtaagattac cggtaactac gatggtgttg aagaagcctg 420 gagttactta gaaaagcaca ttattccaga ttccaagaac caaccaggta acagcagata 480 caacccatct tctccagcta cttacgctgc tgaacatgat gaaatctacg actatccatc 540 caaacttatc ttccaagatg gtcttgttgg tgaagatcca attgccaagg aacttcaaca 600 agcctacggt aactgggata tttacattat gcactggatt attgatggtg ataactggta 660 cggttacggt caacaaggtg atggtactag taagccatcc ttcattaaca ccttccaacg 720 tggtccatcc gaatctactt ggaagactgt tccacaccca tgttgggaag ccatgaagtg 780 gggtggtaga aatggtttct tagatctttt caccgttgat aactcctacg ccaagcaatg 840 gagatacact gctgctccag atgctgatgc tcgtgccatt caagctgctt acttcgccta 900 catgtgggcc gaagaagatg gtgttaacct ttcttctgtt gcttctaagg ctgccaagct 960 tggtgattac ttacgttacg ctcaatacga taagtacttc aagaagattg gtaactgtgt 1020 tggttacgac aagtgttctg ctggtcgtgg taagaactca gctcactacc ttatctcatg 1080 gtacttcgcc tggggtggag gtttacaagg tgactgggct tggagaattg gttcatccca 1140 cactcacact ggttaccaaa acccattagc tgcctggatt ctttctactc aatctgcctt 1200 caagccaaag tcctctactg gtgctaagga ttgggctact tctcttgacc gtcaattaga 1260 attattcaga tggttacaat ccgctgaagg ttgtattgct ggtggtgcca ccaactcatg 1320 gcaaggtgct tacgaacaac catccagtga catcactacc ttctacggta tgtggtacga 1380 ttggcaacca gtttaccacg acccaccatc caacaactgg actggtatgc aaggttgggg 1440 tatggaacgt gtctgttccc tttactacct ttctggtaac gaaaaggccg gtaaggtttg 1500 tcaagaatgg gccaagtggg tcaagaacac cactcgtgtc actggtgaag aaattgttca 1560 cgctactacc cttgattggg aaggtaatcc agatgaatgg aacgcctcta acttcaacaa 1620 gtctaacctc aacagatccc ttcacggtac tgtttcttct gaaggtgttg atcttggtac 1680 cattgcctct atcatgaagg gtttaatgtg ggtttctatg aaggataacg atcaagaagg 1740 tattaacctt gctgtccaag ttatggatgc tattgaaggt tacagagaca accttggtta 1800 ctcttctctt gaagctcgtg gtgattacga aaagttcggt ggagaagttt acattccatc 1860 cggatggact ggtaagaacg ctcaaggtgc taaccttaag aacggtgtta cctttattga 1920 cattcgtcca aagtacaaac aagatccaga ctggccacaa gtcgaagaat tccttaacgg 1980 aggtaaccca ccagaattca actaccacag attctgggct caaactgaaa ttgccgttgc 2040 caatggtctt atttccatct acggattaaa gagcaccggt ggatcttctc caatttacgg 2100 tggtgatgaa gttactgaat gtccagcttc cattactaga caaggttact cttgctgtaa 2160 ggttggatgc caagttgttt accaagatgc tgatggtgac tggggtgttg aaaacaacga 2220 ctggtgtggt tgtggtaagg caccagctcc aaagccaaag tgcccaactt ccattactaa 2280 ccaaggatat tcatgctgtt catcctgtgg tccagtttac taccaagatg ctgatggtga 2340 ctggggtgtt gaaaacggtg actggtgtgg tatgccaact agctgctaaa ttaattaata 2400 ttaataaata ataagatatt agttaatatc tgtaaataga aatattaaaa aattttaaaa 2460 gaccaaatta aattagtaaa aaaaaatgga aaattaggaa atactttaaa aaaatatgat 2520 cccttttttt taaaaaaata agaagtatgt atcaataatt aaataatatt tcaaaagaaa 2580 attgaaatat ttaaataaaa aaaataaaat tttaaaaaat taaaaaaaaa aaaaaaaaaa 2640 aaaaaaaaaa aaaaaaaaaa aaaaaa 2666 // |
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