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EBI DbfetchID AF449412; SV 1; linear; mRNA; STD; FUN; 2742 BP. XX AC AF449412; XX DT 04-DEC-2002 (Rel. 74, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Piromyces sp. E2 cellulase Cel48A precursor (cel48A) mRNA, complete cds. XX KW . XX OS Piromyces sp. E2 OC Eukaryota; Fungi; Neocallimastigomycota; Neocallimastigomycetes; OC Neocallimastigales; Neocallimastigaceae; Piromyces. XX RN [1] RP 1-2742 RX DOI; 10.1080/1042517021000024191 RX PUBMED; 12652902. RA Steenbakkers P.J.M., Freelove A., van Cranenbroek B., Sweegers B.M.C., RA Harhangi H.R., Vogels G.D., Hazlewood G.P., Gilbert H.J., RA Op den Camp H.J.M.; RT "The major component of the cellulosomes of anaerobic fungi from the genus RT Piromyces is a family 48 glycoside hydrolase"; RL DNA Seq. 13(6):313-320(2002). XX RN [2] RP 1-2742 RA Steenbakkers P.J., Freelove A.; RT ; RL Submitted (19-NOV-2001) to the EMBL/GenBank/DDBJ databases. RL Microbiology, University of Nijmegen, Toernooiveld 1, Nijmegen NL-6525 ED, RL The Netherlands XX FH Key Location/Qualifiers FH FT source 1..2742 FT /organism="Piromyces sp. E2" FT /mol_type="mRNA" FT /db_xref="taxon:73868" FT repeat_region 80..100 FT /note="palindrome" FT CDS 139..2400 FT /codon_start=1 FT /gene="cel48A" FT /product="cellulase Cel48A precursor" FT /note="glycosyl hydrolase family 48; corresponds to the FT cellulosome major component" FT /db_xref="GOA:Q8J1E3" FT /db_xref="HSSP:1E8P" FT /db_xref="InterPro:IPR012341" FT /db_xref="UniProtKB/TrEMBL:Q8J1E3" FT /protein_id="AAN76734.1" FT /translation="MPSIRSAIALLGASAVIAAPMKKRANDAYAQRAIEHYNIMTGNGS FT YNSPYFSPENVPYHSVETLMVEAPDHGHESVSETYSFWVWLEAVNGKLTGDYSGVETAW FT SFLEKHMIPDAKNQPGNSKYNPSSPATYAAEHDEITGYPSKLMFQDGIVGEDPIAKELQ FT QAYGTWDIYAMHWIIDGDNWYGYGQQGDGTSKPSFINTFQRGPSESTWKTVPHPCWEAM FT KWGGRNGFLDLFTVDNSYAKQWRYTAAPDADARAIQAAYFGWLWADEDGQDLSSVASKA FT AKLGDYLRYAQYDKYFKKIGNCVGYDRCSAGRGKNSAHYLISWYFAWGGGLQGDWAWRI FT GSSHTHSGYQSPLAAWILSTQAAFKPKSSNGAKDWATSLDRQLELFRWLQTPEGCIAGG FT ATNSWTGAYEQPPSDITTFYGMYYDWQPVYHDPPSNNWTGMQGWGMERVCSLYYLSGNE FT KAGLVCQNWAKWVKKTTRVSNGQIVHATNLSWEGNPDQWNKSNFNKSNLNASLHGTVSS FT EGVDLGSIASIIKGLMWVSMKDGDQEGIDLAVSVMDAIENYKDDLGYAAKEERLDYEKF FT GGEVYIPSGWTGTNAQGANLKNGVTFIDIRPKYKEDPDWPQVENFLNGGNPPAFTYHRF FT WGQTEIMVANGLISIYNLASNNPVKPTNQGTCSAAIKNQGYSCCSANCEVVYQDQDGDW FT GVENGQWCGCGGGEVEVCPSSITAQGYPCCSSCGQVYYQDQDGDWGVENNNWCGMPTNC FT " FT sig_peptide 139..210 FT /gene="cel48A" FT mat_peptide 211..2397 FT /gene="cel48A" FT /product="cellulase Cel48A" FT /note="N-terminal 25 amino acids of extracellular protein FT determined by Edman degradation" FT misc_feature 211..2091 FT /gene="cel48A" FT /note="Region: glycosyl hydrolase family 48 catalytic FT domain" FT misc_feature 2173..2253 FT /gene="cel48A" FT /note="Region: fungal dockerin" FT misc_feature 2272..2397 FT /gene="cel48A" FT /note="Region: fungal dockerin" XX SQ Sequence 2742 BP; 846 A; 510 C; 534 G; 852 T; 0 other; ttttttatta ttatattttt gttaaaaaat taattgttcg cgggaacgaa ctattaaaag 60 aatcaataaa aaaatatttt attattattg aataataata tctcttcagt taaatttatt 120 caaaaaatta attttaaaat gccatctatt agatctgcta ttgctcttct cggtgcttcc 180 gctgttattg ctgctccaat gaagaagcgt gctaacgatg cttacgccca acgtgctatt 240 gaacactaca acatcatgac tggtaatggt tcctacaact ctccatattt ctcaccagaa 300 aatgttccat accactctgt tgaaactctt atggttgaag ctccagatca cggtcacgaa 360 tctgtatctg aaacttactc tttctgggtt tggttagaag ctgttaacgg taagcttact 420 ggtgattact ctggtgttga aactgcctgg agtttcttag aaaagcatat gattccagat 480 gctaagaacc aaccaggtaa cagcaagtac aacccatctt ctccagctac ttacgctgct 540 gaacatgatg aaattactgg ttacccatcc aaacttatgt tccaagacgg tattgttggt 600 gaagatccaa ttgctaagga acttcaacaa gcctacggta cctgggatat ctatgctatg 660 cactggatta ttgatggtga taactggtac ggttacggtc aacaaggtga tggtacttct 720 aagccatctt tcattaacac cttccaacgt ggtccatctg aatctacttg gaagactgtt 780 ccacacccat gttgggaagc catgaaatgg ggtggtcgta acggtttctt agatcttttc 840 actgttgata actcatacgc taagcaatgg agatacactg ctgctccaga tgctgatgct 900 cgtgccattc aagctgctta cttcggttgg ctctgggctg atgaagatgg tcaagatctt 960 tcttctgttg cttctaaggc tgctaagctt ggtgattact tacgttatgc tcaatacgat 1020 aaatacttca agaagatcgg taactgtgtt ggttacgata gatgttctgc tggtcgtggt 1080 aagaactctg ctcactacct tatctcttgg tacttcgctt ggggtggagg tttacaaggt 1140 gactgggcct ggagaattgg ttcctcccac actcacagtg gttaccaatc cccattagct 1200 gcctggattc tttccactca agctgcattc aagccaaagt cttctaatgg tgctaaggat 1260 tgggctactt ctcttgaccg tcaattagaa ttattcagat ggttacaaac tccagaaggt 1320 tgtattgctg gtggtgccac taactcttgg actggtgctt atgaacaacc accaagtgac 1380 attactactt tctatggtat gtactatgac tggcaaccag tttaccacga cccaccatcc 1440 aataactgga ctggtatgca aggttggggt atggaacgtg tctgttcctt atactacctt 1500 tctggtaatg aaaaggctgg tcttgtttgt caaaactggg ctaaatgggt taagaaaact 1560 actcgtgtta gcaatggtca aattgttcat gccactaact tatcttggga aggtaaccca 1620 gatcaatgga acaagagtaa cttcaataag tctaacctta acgcttccct tcacggtact 1680 gtttcttctg aaggtgttga tcttggttca attgcctcta tcattaaggg tttaatgtgg 1740 gtttccatga aggatggtga ccaagaaggt attgaccttg ctgttagtgt tatggatgcc 1800 attgaaaact acaaggacga tcttggttat gctgccaaag aagaacgtct tgactacgaa 1860 aagttcggtg gagaagttta cattccatct ggttggactg gtactaacgc tcaaggtgcc 1920 aaccttaaga acggtgttac tttcattgac attcgtccaa agtacaagga agatccagac 1980 tggccacaag tcgaaaactt ccttaacgga ggtaacccac cagctttcac ttaccacaga 2040 ttctggggtc aaactgaaat catggttgcc aacggtctta tctctatcta caacttagct 2100 tccaataacc cagttaaacc aactaatcaa ggtacttgtt ctgctgctat caagaaccaa 2160 ggttacagtt gctgtagtgc taactgtgaa gttgtttacc aagaccaaga tggtgactgg 2220 ggtgttgaaa acggccaatg gtgtggttgt ggcggtggtg aagtcgaagt ctgcccatct 2280 tctattactg ctcaaggtta tccatgctgt tcatcctgtg gtcaagttta ctaccaagac 2340 caagatggtg attggggtgt tgaaaacaat aactggtgtg gtatgccaac caactgctaa 2400 atgatttaat catttggaaa ttacttttta attttaataa gaatttaact gataattttt 2460 tttaaatgat ttagatataa aaatgatatt tatatcattt aattttaaat tatagttaat 2520 agataacatt ttaaaattaa agaaaagaga ttaaaaaaag tgatcataaa aagtttccat 2580 aatcaaagac caattaattg atgtattgat aaataaagtt aataaaaact aaataaaaaa 2640 aataaaaaaa ttaaaaatat aattaaaaat ataattaaaa aatagagttg aaataaataa 2700 aacaaaaaat taaatatata aaataaaaaa aaaaaaaaaa aa 2742 // |
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