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EBI DbfetchID AF442961; SV 1; linear; genomic DNA; STD; PRO; 2892 BP. XX AC AF442961; XX DT 24-OCT-2002 (Rel. 73, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Halothermothrix orenii alpha-amylase A (amyA) gene, complete cds. XX KW . XX OS Halothermothrix orenii OC Bacteria; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; OC Halothermothrix. XX RN [1] RP 1-2892 RX PUBMED; 12177328. RA Mijts B.N., Patel B.K.; RT "Cloning, sequencing and expression of an alpha-amylase gene, amyA, from RT the thermophilic halophile Halothermothrix orenii and purification and RT biochemical characterization of the recombinant enzyme"; RL Microbiology (Reading, Engl.) 148(Pt 8):2343-2349(2002). XX RN [2] RP 1-2892 RA Mijts B.N., Patel B.K.C.; RT ; RL Submitted (04-NOV-2001) to the EMBL/GenBank/DDBJ databases. RL Biomolecular and Biomedical Sciences, Griffith University, Kessels Rd, RL Brisbane, QLD 4111, Australia XX FH Key Location/Qualifiers FH FT source 1..2892 FT /organism="Halothermothrix orenii" FT /mol_type="genomic DNA" FT /db_xref="taxon:31909" FT gene 359..1978 FT /gene="amyA" FT -35_signal 359..365 FT /gene="amyA" FT /inference="non-experimental evidence, no additional FT details recorded" FT -10_signal 383..388 FT /gene="amyA" FT /inference="non-experimental evidence, no additional FT details recorded" FT RBS 415..423 FT /gene="amyA" FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 431..1978 FT /codon_start=1 FT /transl_table=11 FT /gene="amyA" FT /product="alpha-amylase A" FT /note="AmyA" FT /db_xref="GOA:Q8GPL8" FT /db_xref="InterPro:IPR013781" FT /db_xref="PDB:1WZA" FT /db_xref="UniProtKB/TrEMBL:Q8GPL8" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAN52525.1" FT /translation="MKLKRLSFFMFVTLLVFISVFPVYANDFEKHGTYYEIFVRSFYDS FT DGDGIGDLKGIIEKLDYLNDGDPETIADLGVNGIWLMPIFKSPSYHGYDVTDYYKINPD FT YGTLEDFHKLVEAAHQRGIKVIIDLPINHTSERHPWFLKASRDKNSEYRDYYVWAGPDT FT DTKETKLDGGRVWHYSPTGMYYGYFWSGMPDLNYNNPEVQEKVIGIAKYWLKQGVDGFR FT LDGAMHIFPPAQYDKNFTWWEKFRQEIEEVKPVYLVGEVWDISETVAPYFKYGFDSTFN FT FKLAEAVIATAKAGFPFGFNKKAKHIYGVYDREVGFGNYIDAPFLTNHDQNRILDQLGQ FT DRNKARVAASIYLTLPGNPFIYYGEEIGMRGQGPHEVIREPFQWYNGSGEGETYWEPAM FT YNDGFTSVEQEEKNLDSLLNHYRRLIHFRNENPVFYTGKIEIINGGLNVVAFRRYNDKR FT DLYVYHNLVNRPVKIKVASGNWTLLFNSGDKEITPVEDNNKLMYTIPAYTTIVLEKE" XX SQ Sequence 2892 BP; 814 A; 524 C; 629 G; 925 T; 0 other; gatcctgagc ttattatttc ggttaaatcc ctgggggcag ggagtatgca gatataccgg 60 gaagtaataa ttccagccac tttccctaaa atttttacgg ctaccagaat aagtattggt 120 acagctatag ctgtcctttt cctgaccgaa acctttgcta ctttcagggg actggggtac 180 tttattatgg attgctggac cagggtcaac tatactgaaa tgtatgccgg gattttaatt 240 ttaagccttc ttggcctctc cctgtttctg ttgatagacc tgctggagcg tattatctgc 300 ccctggttat ttattgacag gggtatatag tccagtggta ggctggatta tataatttat 360 tgaaattttc gataaattcc gttataataa tattaaatct gaaaatattt agtaaaagga 420 tgtgagatat gtgaagctaa aacgcttgtc tttcttcatg tttgttacct tactggtatt 480 tatatctgtt tttcctgttt atgcaaacga tttcgaaaaa catggtacat attatgaaat 540 ttttgtcagg tctttttatg actctgatgg tgacggaata ggggatttga aaggtataat 600 agaaaaactg gattatctta atgatggaga ccctgaaacc attgctgatc tgggggttaa 660 tggtatctgg ttaatgccta tctttaaatc tccctcctat catggctatg atgtaaccga 720 ttattataag attaatcctg actacgggac tctggaagac ttccataagc ttgttgaggc 780 tgcccatcaa aggggaatca aggttataat tgatttaccc atcaatcata ccagtgaaag 840 acatccctgg tttctcaagg cttcccggga taagaatagt gaatacaggg attattatgt 900 ctgggctggc cccgataccg ataccaaaga aaccaagtta gatggaggcc gggtctggca 960 ttattccccg accggcatgt attatgggta tttctggagt ggcatgcctg atttaaacta 1020 taataaccct gaagttcagg aaaaggttat tgggatagca aaatactggt taaaacaggg 1080 ggttgatggt ttcaggcttg atggagccat gcatatcttc ccaccggccc agtatgataa 1140 aaactttacc tggtgggaga agttccgtca ggaaattgaa gaggtaaaac ccgtttacct 1200 ggtgggtgag gtctgggata tttcggaaac ggtagctcct tacttcaaat atggttttga 1260 ttctaccttt aactttaaac tggcagaggc agttatcgct acggctaaag ctggatttcc 1320 ctttggtttt aataaaaagg caaaacatat ttacggggta tatgacaggg aggttggatt 1380 tgggaattat atcgatgctc ccttcctgac caaccatgat cagaaccgga ttttggacca 1440 gcttgggcag gatcgtaata aggccagggt tgctgccagt atttatttga ccttgcctgg 1500 taatcccttt atttactatg gtgaagaaat cggtatgagg gggcaggggc cccatgaagt 1560 tatcagggag cccttccagt ggtataatgg atccggggag ggagaaacat actgggagcc 1620 agccatgtat aatgatggct ttacttctgt tgaacaggaa gaaaagaatc tcgattccct 1680 cttaaatcac tacaggaggt taatccattt ccggaacgaa aatcctgtct tttataccgg 1740 taagattgag attataaatg gaggattaaa tgtagttgca tttagaagat ataatgataa 1800 gagggattta tatgtctacc ataacctggt aaacagaccg gttaaaataa aagtggctag 1860 tggtaactgg accttattgt ttaattcagg tgataaggaa attacccctg ttgaagataa 1920 taataaactt atgtatacta tccctgctta tactaccatt gttctggaaa aggagtaaag 1980 ggagagggtg agtaataatt atgaaaagga gagggtaaat ccctctcctt ttttgttaaa 2040 tatttttatt atacctgtta taattgattt ttttgatgtt atttattttt taaattgaga 2100 tagaactgaa ctaattggtc tgagtttgat ttgatgtcag ctcaatttca cccagtgttg 2160 taacctcacc ggctgtaacc tggacatctt cgattattgt tgattcatca tagttgtcgg 2220 cagtaatttc aaggtcatag gttccttcca cgaggccgag aagtttaaag ccaccttcat 2280 taccactatt ggtagaagtg acatacccct cttcagtaaa ttcatcattg tgataaactg 2340 taatgggtgc ttcagcaata ccattaccat tactgtcaac caccttaccc tggatatctc 2400 ctaagatccc tttttcacat aatccttata gttggtttaa gtttatattt attatttgga 2460 taaattagag actttctggc atcaaaatca agcaacagga cggtaccctt gtctggagaa 2520 acagtaaatg agttgaccag tttaataccg gtctgatctg cagaaggaat atcgacaggt 2580 tctgtactat ctttaccaga ttcttgtacc cgggctttgg agacataaac cctcatttga 2640 gtgtagtgtc cttcaggtaa atagtctttg ccgattaatt cagaaacccc ctgaagttct 2700 aataaatcaa actctttttc ctcttccagg attgtgaacc agccctcttc gtcgttatgt 2760 gtttctattc tcgatatggt taccagtaat ttttctacat tatcggccgg ggcatcggtc 2820 aggagtagtt ggatttcacc ggtttctttg ccaaaaaaag acaggttatc acaggctgtc 2880 agccccagga tc 2892 // |
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