EBI Dbfetch

ID   AF439804; SV 1; linear; genomic DNA; STD; PRO; 1854 BP.
XX
AC   AF439804;
XX
DT   08-MAR-2002 (Rel. 71, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 3)
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DE   Pasteurella multocida isolate P-4058 heparosan synthase A (hssA) gene,
DE   complete cds.
XX
KW   .
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OS   Pasteurella multocida
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
XX
RN   [1]
RP   1-1854
RX   DOI; 10.1074/jbc.M112130200
RX   PUBMED; 11756462.
RA   DeAngelis P.L., White C.L.;
RT   "Identification and molecular cloning of a heparosan synthase from
RT   Pasteurella multocida type D";
RL   J. Biol. Chem. 277(9):7209-7213(2002).
XX
RN   [2]
RP   1-1854
RA   DeAngelis P.L., White C.L.;
RT   ;
RL   Submitted (24-OCT-2001) to the EMBL/GenBank/DDBJ databases.
RL   Biochem. & Molec. Biol., Univ. of Oklahoma, 940 Stanton L. Young Blvd.,
RL   Oklahoma City, OK 73104, USA
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1854
FT                   /organism="Pasteurella multocida"
FT                   /host="rabbit"
FT                   /isolate="P-4058"
FT                   /mol_type="genomic DNA"
FT                   /note="type: D"
FT                   /db_xref="taxon:747"
FT   gene            1..1854
FT                   /gene="hssA"
FT   CDS             1..1854
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="hssA"
FT                   /product="heparosan synthase A"
FT                   /function="catalyzes the addition of GlcNac and GlcUA to
FT                   form the (alpha4-GlcNac-beta4 GlcUA) polymer backbone of
FT                   N-acetylheparosan (desulfated, unepimerized heparin)"
FT                   /note="PmHS1; glycosyltransferase; polymerase; synthesizes
FT                   the capsular polysaccharide of bacteria from UDP-sugar
FT                   precursors"
FT                   /db_xref="InterPro:IPR001173"
FT                   /db_xref="UniProtKB/TrEMBL:Q8RP78"
FT                   /protein_id="AAL84705.1"
FT                   /translation="MSLFKRATELFKSGNYKDALTLYENIAKIYGSESLVKYNIDICKK
FT                   NITQSKSNKIEEDNISGENKFSVSIKDLYNEISNSELGITKERLGAPPLVSIIMTSHNT
FT                   EKFIEASINSLLLQTYNNLEVIVVDDYSTDKTFQIASRIANSTSKVKTFRLNSNLGTYF
FT                   AKNTGILKSKGDIIFFQDSDDVCHHERIERCVNALLSNKDNIAVRCAYSRINLETQNII
FT                   KVNDNKYKLGLITLGVYRKVFNEIGFFNCTTKASDDEFYHRIIKYYGKNRINNLFLPLY
FT                   YNTMREDSLFSDMVEWVDENNIKQKTSDARQNYLHEFQKIHNERKLNELKEIFSFPRIH
FT                   DALPISKEMSKLSNPKIPVYINICSIPSRIKQLQYTIGVLKNQCDHFHIYLDGYPEVPD
FT                   FIKKLGNKATVINCQNKNESIRDNGKFILLEKLIKENKDGYYITCDDDIRYPADYINTM
FT                   IKKINKYNDKAAIGLHGVIFPSRVNKYFSSDRIVYNFQKPLENDTAVNILGTGTVAFRV
FT                   SIFNKFSLSDFEHPGMVDIYFSILCKKNNILQVCISRPSNWLTEDNKNTETLFHEFQNR
FT                   DEIQSKLIISNNPWGYSSIYPLLNNNANYSELIPCLSFYNE"
XX
SQ   Sequence 1854 BP; 766 A; 253 C; 261 G; 574 T; 0 other;
     atgagcttat ttaaacgtgc tactgagcta tttaagtcag gaaactataa agatgcacta        60
     actctatatg aaaatatagc taaaatttat ggttcagaaa gccttgttaa atataatatt       120
     gatatatgta aaaaaaatat aacacaatca aaaagtaata aaatagaaga agataatatt       180
     tctggagaaa acaaattttc agtatcaata aaagatctat ataacgaaat aagcaatagt       240
     gaattaggga ttacaaaaga aagactagga gccccccctc tagtcagtat tataatgact       300
     tctcataata cagaaaaatt cattgaagcc tcaattaatt cactattatt gcaaacatac       360
     aataacttag aagttatcgt tgtagatgat tatagcacag ataaaacatt tcagatcgca       420
     tccagaatag caaactctac aagtaaagta aaaacattcc gattaaactc aaatctaggg       480
     acatactttg cgaaaaatac aggaatttta aagtctaaag gagatattat tttctttcag       540
     gatagcgatg atgtatgtca ccatgaaaga atcgaaagat gtgttaatgc attattatcg       600
     aataaagata atatagctgt tagatgtgca tattctagaa taaatctaga aacacaaaat       660
     ataataaaag ttaatgataa taaatacaaa ttaggattaa taactttagg cgtttataga       720
     aaagtattta atgaaattgg tttttttaac tgcacaacca aagcatcgga tgatgaattt       780
     tatcatagaa taattaaata ctatggtaaa aataggataa ataacttatt tctaccactg       840
     tattataaca caatgcgtga agattcatta ttttctgata tggttgagtg ggtagatgaa       900
     aataatataa agcaaaaaac ctctgatgct agacaaaatt atctccatga attccaaaaa       960
     atacacaatg aaaggaaatt aaatgaatta aaagagattt ttagctttcc tagaattcat      1020
     gacgccttac ctatatcaaa agaaatgagt aagctcagca accctaaaat tcctgtttat      1080
     ataaatatat gctcaatacc ttcaagaata aaacaacttc aatacactat tggagtacta      1140
     aaaaaccaat gcgatcattt tcatatttat cttgatggat atccagaagt acctgatttt      1200
     ataaaaaaac tagggaataa agcgaccgtt attaattgtc aaaacaaaaa tgagtctatt      1260
     agagataatg gaaagtttat tctattagaa aaacttataa aggaaaataa agatggatat      1320
     tatataactt gtgatgatga tatccggtat cctgctgact acataaacac tatgataaaa      1380
     aaaattaata aatacaatga taaagcagca attggattac atggtgttat attcccaagt      1440
     agagtcaaca agtatttttc atcagacaga attgtctata attttcaaaa acctttagaa      1500
     aatgatactg ctgtaaatat attaggaact ggaactgttg cctttagagt atctattttt      1560
     aataaatttt ctctatctga ttttgagcat cctggcatgg tagatatcta tttttctata      1620
     ctatgtaaga aaaacaatat actccaagtt tgtatatcac gaccatcgaa ttggctaaca      1680
     gaagataaca aaaacactga gaccttattt catgaattcc aaaatagaga tgaaatacaa      1740
     agtaaactca ttatttcaaa caacccttgg ggatactcaa gtatatatcc attattaaat      1800
     aataatgcta attattctga acttattccg tgtttatctt tttataacga gtaa            1854
//