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EBI DbfetchID AF434798; SV 1; linear; genomic DNA; STD; PRO; 6389 BP. XX AC AF434798; XX DT 03-MAY-2002 (Rel. 71, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Corynebacterium glutamicum prpDBC1 gene cluster, complete sequence. XX KW . XX OS Corynebacterium glutamicum OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Corynebacteriaceae; Corynebacterium. XX RN [1] RP 1-6389 RX DOI; 10.1128/JB.184.10.2728-2739.2002 RX PUBMED; 11976302. RA Claes W.A., Puhler A., Kalinowski J.; RT "Identification of two prpDBC gene clusters in Corynebacterium glutamicum RT and their involvement in propionate degradation via the 2-methylcitrate RT cycle"; RL J. Bacteriol. 184(10):2728-2739(2002). XX RN [2] RP 1-6389 RA Claes W.A., Kalinowski J., Puehler A.; RT ; RL Submitted (15-OCT-2001) to the EMBL/GenBank/DDBJ databases. RL Department of Genetics, University of Bielefeld, Universitaetsstr. 25, RL Bielefeld, D 33615, Germany XX DR StrainInfo; 52176; ATCC 13032. XX FH Key Location/Qualifiers FH FT source 1..6389 FT /organism="Corynebacterium glutamicum" FT /strain="ATCC 13032" FT /mol_type="genomic DNA" FT /db_xref="taxon:1718" FT CDS complement(22..1110) FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="Orf1; similar to conserved hypothetical protein yciC FT of Bacillus subtilis" FT /db_xref="HSSP:1NIJ" FT /db_xref="InterPro:IPR003495" FT /db_xref="InterPro:IPR011629" FT /db_xref="UniProtKB/TrEMBL:Q8NSI1" FT /protein_id="AAM21498.1" FT /translation="MATTTPVTVLSGFLGSGKTTLLNQILHHRGSRKIAVIVNDFSEIN FT IDAALIASEGHLTRGEDRFVELTNGCICCTLREDLVDSVGELASSDRFDHIVIESTGIS FT EPMPVAATFEWQWDDGTRLADKAPIDTMVTLVDATQFIDLIRKNTSLTEADMGATEDDE FT RTIADLLTDQIEFADRIYITKSDLVDRTVLEQTRALIASMSPRARIDLLIDGLNDGSPI FT TDDILGAFLYDEATARAYEGYTEELENPHTPETEEYGISSVVFRSDRPFNKDRLLQVLR FT STTGLVRSKGYCWIADHLNIVQVWHQAGPNLSIRPAAYWANSEITPGTELVLIGIHIDG FT PTLLALLQGATLTDAEVAALVL" FT CDS 1229..2155 FT /codon_start=1 FT /transl_table=11 FT /product="putative oxidoreductase" FT /note="Orf2" FT /db_xref="GOA:Q8NSI0" FT /db_xref="InterPro:IPR000103" FT /db_xref="InterPro:IPR013027" FT /db_xref="UniProtKB/TrEMBL:Q8NSI0" FT /protein_id="AAM21499.1" FT /translation="MSSHDLVDVVVVGAGAAGLAAAVALGRSLRSVIVIDAGQPRNSYA FT HAAHNVLGQEGIAPAELLEKGRAEARSYGVTIAPGRVAKVERTGSTFAITLDDASLLHS FT RRIILAHGAVDDLPEVEGLSDFWGTKVLHCAYCHGFEARDSEIVVVGTSPMAAHQALMF FT SQLSKTVSLVGTIDIDEQTSESLDSAGVKVLGTNAVRVSAEGDGLSVELSEGDHLSCDN FT IVVASRPLVDGTLYTQLGGQMEENPMGRFIPGTQTGRTPIEGVWAAGNAQAPMAMVYGS FT AAQGVMAGAEINFDLILEDISVASAQS" FT CDS 2243..3739 FT /codon_start=1 FT /transl_table=11 FT /gene="prpD1" FT /product="PrpD1" FT /note="putative 2-methylcitrate dehydratase" FT /db_xref="GOA:Q8NSH9" FT /db_xref="InterPro:IPR005656" FT /db_xref="UniProtKB/Swiss-Prot:Q8NSH9" FT /protein_id="AAM21500.1" FT /translation="MRIHDVYTHLSADNFPKAEHLAWKFSELATDPVEVTPDVSEMIIN FT RIIDNAAVSAASVLRRPVTVARQQAQSHPREKGGKVFGISGSYSPEWAAFANGVAVREL FT DFHDTFLAAEYSHPGDNIPPLLAVAQAQRSSGRDLIRGIATAYEVQVELVRGICLHEHK FT IDHVAHLGPSAAAGLGTLLHVDEETIYQAIGQALHTTTATRQSRKGEISSWKAFAPAFA FT GKMAIEAMDRAMRGEGSPAPIWEGEDGVIAWLLSGKDHVYHVPLPEHGEPKLGILETYT FT KEHSAEYQSQAPIDLARRMKPLVDAAGGTEHIAEIVLRTSHHTHYVIGTGANDPQKMDP FT QASRETLDHSIMYIFAVALQDGVWHHEFSYTRKRSTRPETVELWHKIRTVEDPEWTRRY FT HSDDPAKKAFGAKAVITMADGTVIEDELAVADAHPLGARPFARENYIEKFRTLAQGIVI FT DSEQERFLHAVQSLPDLDDLDQLNIEVDISNQAATKAGLL" FT CDS 3736..4653 FT /codon_start=1 FT /transl_table=11 FT /gene="prpB1" FT /product="PrpB1" FT /note="putative 2-methylisocitrate lyase" FT /db_xref="GOA:Q8NSH8" FT /db_xref="InterPro:IPR012695" FT /db_xref="InterPro:IPR015813" FT /db_xref="InterPro:IPR018523" FT /db_xref="UniProtKB/Swiss-Prot:Q8NSH8" FT /protein_id="AAM21501.1" FT /translation="MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLIARSIEEAG FT FEGVYVSGAVIAADLALPDIGLTTLTEVAHRARQIARVTDLGVLVDADTGFGEPMSAAR FT TVAELEDAGVAGCHLEDQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVICAR FT TDAAGVEGIDAAIERAKAYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGK FT TTLLSADVLEEIGYNAVIYPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYE FT LLRYEDYNVFDQHIFTYRKGENNE" FT CDS 4646..5791 FT /codon_start=1 FT /transl_table=11 FT /gene="prpC1" FT /product="PrpC1" FT /note="2-methylcitrate synthase" FT /db_xref="GOA:Q8NSH7" FT /db_xref="InterPro:IPR002020" FT /db_xref="InterPro:IPR011278" FT /db_xref="InterPro:IPR016141" FT /db_xref="InterPro:IPR016142" FT /db_xref="InterPro:IPR019810" FT /db_xref="UniProtKB/Swiss-Prot:Q8NSH7" FT /protein_id="AAM21502.1" FT /translation="MSDSQVRKGLNGVISDYTSISKVMPESNSLTYRGYAVEDLVENCS FT FEEVIYLLWFGELPTTEQLRTFNTTGRSYRSLDAGLISLIHSLPNTCHPMDVLRTAVSY FT MGTFDPDPFTRDADHIRSIGHNLLAQLPMVVAMDIRRRSGEEIIAPDHNKGIASNFLSM FT VFGNDDGSVANSADDIRDFERSLILYAEHSFNASTFSARVISSTRSDTYSAITGAIGAL FT KGPLHGGANEFVMHTMLDIDDPNNAADWMGKALDRKERIMGFGHRVYKNGDSRVPSMEK FT SMRSLAARHRGQKWVHMYESMQEVMEARTGIKPNLDFPAGPAYYMLGFPVDFFTPLFVL FT ARVSGWTAHIVEQFENNALIRPLSAYNGVEEREVVPISERT" XX SQ Sequence 6389 BP; 1382 A; 1727 C; 1892 G; 1388 T; 0 other; gaattcacgg acttgaggaa ctcacaaaac cagtgccgcc acttccgcat cggtaagcgt 60 cgcgccttgt agcagtgcga gcaaggtggg gccgtcgatg tggatgccga tcaggaccaa 120 ttcagtgccg ggggtgatct ctgaattggc ccaataggcg gccgggcgaa tgctcaaatt 180 ggggccggct tggtgccaga cctggacgat gttgaggtga tcagcgatcc agcaatagcc 240 tttggagcga acgagtccag tggtggaacg cagtacctga agtaggcgat ccttgttgaa 300 cggccggtca gagcggaata ccacggaact gatgccgtat tcctcggttt cgggagtgtg 360 cgggttttcg agttcttccg tgtagccctc gtaggcgcgg gcggtggctt cgtcgtagag 420 gaaagctccg aggatgtcgt cggtgatggg ggagccgtca ttgaggccat cgatgagtaa 480 gtcaatgcgg gcgcgtggac tcatgctggc gattagtgcg cgggtttgct cgaggacagt 540 gcggtccacc aggtcggatt tggtgatgta gatgcggtcg gcgaactcga tttgatcggt 600 gagtaggtcg gcgatggtgc gttcatcgtc ctcggttgcg cccatgtcag cctcggtgag 660 ggaggtgttt ttgcggatga ggtcgatgaa ttgggtggca tcgaccaggg tgaccatggt 720 gtcgatcggt gctttgtcag ccaggcgggt gccgtcatcc cactgccatt caaatgtggc 780 ggctaccggc attggttcgg agatacccgt ggattcgatg acgatgtggt cgaagcggtc 840 gctgctggcg agttcgccga cggaatccac gaggtcttcg cgcagggtgc agcagatgca 900 tccgttggtc agttccacga agcggtcttc gccgcgggtg aggtggcctt cggatgcgat 960 gagggctgcg tcgatgttga tttctgaaaa atcgttgaca atgactgcga ttttccggga 1020 tcctcggtgg tggaggatct ggttgagcaa tgtggttttt ccggagccga gaaatcctga 1080 tagcacggtg actggggtcg ttgtggccat gtgaaagccc tccttttggg aatcattttc 1140 aatagagtcg acgcaagtgt acacttctta atggaaattg ttttcaataa agtcaagttt 1200 tttgaccttc gctttttagg agcaccccat gtcatctcat gatctcgttg acgtagttgt 1260 cgtcggcgcc ggcgctgcag gtctcgccgc cgctgtcgcg ctcggccgct cactgcgcag 1320 tgtcatcgtc atcgacgctg gtcaaccccg taacagctat gcgcacgctg ctcacaatgt 1380 cctcggccag gaaggcattg cgcccgccga gctgctggaa aaaggccgcg ccgaagcgcg 1440 ttcctatggc gtcaccattg cgcccgggcg cgtagcaaaa gttgagcgca ccggttccac 1500 cttcgccata acgcttgacg acgcctccct ccttcactct cggcgcatca ttttggccca 1560 cggcgccgtt gacgatctgc cagaggtaga aggactgtca gatttttggg gaaccaaagt 1620 gttgcactgc gcttactgcc acggctttga ggcccgcgat tctgaaatcg tcgtggtggg 1680 tacctcgccc atggctgcgc accaagcgtt gatgttctcg cagttgtcca aaactgtcag 1740 cttggtgggc acgatcgaca ttgatgaaca aaccagcgag agcctagata gtgctggagt 1800 aaaagtgttg ggcaccaatg cggtgcgcgt atccgccgaa ggtgatggcc tgtctgtgga 1860 actgtccgaa ggcgatcatt taagctgcga caacatcgtg gtggcatctc gtccactggt 1920 ggatggcacg ctgtacaccc aacttggtgg tcagatggaa gaaaacccga tgggcaggtt 1980 cattccaggt acccaaaccg ggcgcactcc tattgaaggt gtgtgggctg ccggaaacgc 2040 gcaagctccc atggcgatgg tctatggttc cgctgctcaa ggcgtgatgg ctggagcaga 2100 gatcaacttt gatctgatcc tggaagatat ttccgtggca agcgcgcaga gctaaactgc 2160 gtgaggttgt ggcctgtcac acataatcgg cctagggtgg gactttaagg aaacagtgca 2220 caaataaatc tcaaggagcc ccatgcgcat ccacgatgtt tatacccacc tttcggccga 2280 taactttccc aaagcagagc accttgcgtg gaaattctcc gagcttgcca ccgaccccgt 2340 ggaggtgaca ccggatgttt cggagatgat catcaaccgg atcatcgaca acgcggcggt 2400 gtctgccgcg tcggtgttgc gccggcctgt gactgtggcc aggcaacaag cgcagtccca 2460 tccgcgggaa aagggcggaa aagtttttgg aatttcaggc agctactcac cagagtgggc 2520 tgcctttgct aatggtgtgg ccgtacgtga attggacttc cacgatacat ttttagcagc 2580 tgaatactcc catcccggcg acaatattcc accacttctt gcagtagcgc aggctcagag 2640 aagcagcggc agggatctca ttcggggtat cgccactgcc tatgaggtgc aggtggaatt 2700 ggtgaggggg atctgcttgc atgagcacaa aattgatcac gtagcccacc ttggtcccag 2760 cgctgcagcg ggtttgggca cgttgttgca tgtagatgag gaaaccatct accaggcgat 2820 cggccaggca ttgcacacca cgacggctac gaggcagtcg cgaaaaggtg agatttccag 2880 ctggaaggcg ttcgcgccag cgtttgcggg aaagatggcc attgaggcga tggatcgtgc 2940 gatgcgtggg gagggttcgc ccgcaccgat ttgggagggc gaagacgggg tcatcgcgtg 3000 gctgttatcg ggcaaagatc atgtttatca tgtgccattg ccggaacacg gcgagcccaa 3060 gctggggatt ctagagactt acacaaagga acattcagcg gaatatcaat cgcaggcacc 3120 gattgatctg gcgcgcagga tgaagccact ggttgacgcg gctggcggaa cggaacacat 3180 tgcagagatt gtgctgcgca ccagtcacca cacgcattat gtgattggca ctggggcgaa 3240 cgatccgcag aagatggatc cgcaggcctc gcgtgaaacc ctggatcatt ccatcatgta 3300 cattttcgcc gtcgcgcttc aagatggcgt gtggcaccac gagttttcct acacccgcaa 3360 gcgttccacc cgcccggaaa ctgtggagct gtggcacaag attcgcaccg tggaggatcc 3420 tgaatggacg cgccgatacc attctgatga tcctgcaaaa aaggcctttg gtgcgaaagc 3480 agtgatcaca atggctgatg gcaccgtgat tgaggatgaa ttggctgtcg cggatgccca 3540 cccgctgggt gctcggccgt ttgcgcggga gaattacatt gaaaaattcc gcacactcgc 3600 gcaggggatt gtcattgatt cagaacagga acgcttcttg catgccgtgc aaagcctgcc 3660 tgacctggat gatcttgatc agctcaacat cgaagtcgac ataagcaacc aggccgcgac 3720 gaaagcgggg ctgttatgaa tctcttttcg aatggtgttg atgtggggag gcgtcgacaa 3780 gcatttaaag cggcactcgc cgcaccccac atcgcccggc tgcccggcgc attctcccct 3840 ctgattgcgc gctccatcga agaagccggc ttcgaaggcg tctacgtttc cggcgccgtc 3900 atagctgctg acctggcact acccgatatc ggcttgacga cgctgaccga agtcgcccac 3960 cgcgcgcggc aaattgcgcg cgtcacagac ctaggagtgc ttgtcgacgc cgacaccggc 4020 tttggcgaac ccatgtcggc cgcacgcacc gtcgccgaat tggaggacgc cggtgtggcc 4080 ggatgccacc ttgaagacca agtcaacccc aaacgttgcg ggcacttgga cggcaaagaa 4140 gtcgtgcgca cagacgtgat ggttcgacgc atcgcagccg ccgtctcggc ccggcgcgac 4200 ccgaactttg tcatctgcgc ccgcaccgac gccgctggag tggaaggaat cgacgccgcc 4260 attgagcgcg cgaaagccta cttagatgcg ggcgccgaca tgattttcac cgaagccctc 4320 cacagcgaag ccgacttccg atacttccgg cacgccatcc ctgatgcctt gttgctggcg 4380 aatatgaccg aatttggcaa aacgacgctg ctgtcagccg acgtgttgga agagattggc 4440 tacaacgccg tgatctaccc cgtgaccacg ctgcgtattg ccatgggaca agtagaacaa 4500 gcactagccg aaatcaaaga acacggtacc caagaaggat ggctggaccg catgcaacac 4560 cgcagcagat tatatgagct cctgcgatac gaagactaca acgtctttga ccagcacatt 4620 ttcacctaca gaaaaggaga aaacaatgag tgacagccaa gtccgcaaag gactcaacgg 4680 cgtcatctct gactacacaa gcatttccaa agtgatgcca gagagcaact cgctgactta 4740 ccgtggctac gccgtggagg atttggtgga aaactgcagc tttgaagaag tgatctacct 4800 cctgtggttt ggggagctgc ccaccactga acaactccgg accttcaaca cgacaggtcg 4860 aagctaccgc tcactcgacg ccggactgat ttccctcatc cactccttac ccaacacctg 4920 ccaccccatg gacgtgctgc gcaccgcagt gtcctacatg ggtacctttg atcccgatcc 4980 gtttacccgc gatgccgatc atatccgaag cattggacac aacctgcttg cgcagcttcc 5040 catggtggtt gccatggata tccgcaggcg aagtggggaa gagatcatcg cacctgacca 5100 caacaaaggt atcgcttcga atttcttatc catggtgttt ggcaatgatg atggttctgt 5160 agccaactcc gcagatgaca tccgcgattt tgaacgctcc ctcatcctct acgccgagca 5220 ctccttcaac gcctccacat tctcagcccg cgtgatctca tcaacgcgat ccgatacgta 5280 ttcggcgatc acaggtgcga tcggtgctct caaaggccca ctgcacggag gtgccaatga 5340 gtttgtcatg cacaccatgc tggatatcga cgatcccaac aatgctgccg actggatggg 5400 caaggcgttg gatcgtaaag aacgcatcat gggattcggg caccgcgtgt acaaaaacgg 5460 cgactccagg gtcccctcca tggagaaatc catgcgctcc cttgctgctc gtcaccgtgg 5520 tcaaaaatgg gtgcacatgt atgagtcgat gcaagaagtc atggaggctc gcactggcat 5580 taaacccaac ctcgacttcc cggccggccc tgcctattac atgctgggat tccccgtcga 5640 cttcttcaca ccactgtttg tgctggcccg agtgtcaggg tggacggcac acatcgtgga 5700 gcaatttgaa aacaatgcgc tgatccgacc attgtctgcc tacaacggag tggaagaaag 5760 ggaggtggtg cccatttcgg agagaaccta atcagtgagg ctgatttcta aatagccttg 5820 tgcccatctg gtgatggtgt cgcggccctc cgccacagca tcgccgatgg ttttcggagc 5880 gagcactgga tcattgggca attgggtggc gggatcacct gcgggcacga cctcgagtgt 5940 gcgctgcttg ggatcaaggc gtgccagtgc cagtagttca acactggtct gctcgcagat 6000 agcccaggtg tattcggtgt cctcgtgcca ggctgtggtg gcgtccgcga tgaactgtgc 6060 tgcatcggca aggatcagtg agagcgccgg gacatcatca atccgattat ctgaggtgag 6120 cttgcgggcg tagaaacgac cgccgtttag ttccatgggt tccatagtgt tttttaagtg 6180 ctgccttttt agttgaggag ttggacaggg gctgggcgga tagattcttg acccgatggt 6240 gggaatgcac gctgcacaca gttctcccga ggacagatcc gacaaccagg gccgatctct 6300 gcggcggact cgggatccaa attaaggcct tgggaataca ccaagcctgg tgcctcgctg 6360 atatcgcagc ccaggccgat ggcgaattc 6389 // |
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