EBI Dbfetch

ID   AF351622; SV 1; linear; genomic DNA; STD; PRO; 1230 BP.
XX
AC   AF351622;
XX
DT   11-APR-2001 (Rel. 67, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   Amycolatopsis orientalis glycosyltransferase GtfE (gtfE) gene, complete
DE   cds.
XX
KW   .
XX
OS   Amycolatopsis orientalis
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Pseudonocardineae; Pseudonocardiaceae; Amycolatopsis.
XX
RN   [1]
RP   1-1230
RX   DOI; 10.1021/bi010050w
RX   PUBMED; 11294642.
RA   Losey H.C., Peczuh M.W., Chen Z., Eggert U.S., Dong S.D., Pelczer I.,
RA   Kahne D., Walsh C.T.;
RT   "Tandem action of glycosyltransferases in the maturation of vancomycin and
RT   teicoplanin aglycones: novel glycopeptides";
RL   Biochemistry 40(15):4745-4755(2001).
XX
RN   [2]
RP   1-1230
RA   Losey H.C., Walsh C.T.;
RT   ;
RL   Submitted (21-FEB-2001) to the EMBL/GenBank/DDBJ databases.
RL   Biological Chemistry and Molecular Pharmacology, Harvard Medical School,
RL   240 Longwood Ave., Boston, MA 02115, USA
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1230
FT                   /organism="Amycolatopsis orientalis"
FT                   /strain="ATCC19795"
FT                   /mol_type="genomic DNA"
FT                   /note="vancomycin producer"
FT                   /db_xref="taxon:31958"
FT   CDS             1..1230
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="gtfE"
FT                   /product="glycosyltransferase GtfE"
FT                   /db_xref="GOA:Q9AFC6"
FT                   /db_xref="HSSP:1IIR"
FT                   /db_xref="InterPro:IPR002213"
FT                   /db_xref="UniProtKB/TrEMBL:Q9AFC6"
FT                   /protein_id="AAK31353.1"
FT                   /translation="MRVLLSTCGSRGDVEPLVALAVRLRARGAEVRMCAPPDCADRLAE
FT                   VGVPHLPLGRSARPAAAGEAKPLTAEDMLRFTTETIAMQFDRIPAAAEGCDAVVTTGLL
FT                   AAALGVRSVAEKLGIPYFYAFHCPSYVPSPYYAPPPPLGEPPAPEGTDIRALWERNNQS
FT                   AFRRYGAPLKSQRAAIGLPPVEDIFEHGYTDHPWMAADQVLAPLQPTDLDAVQTGAWIL
FT                   PDERPLSPEMEAFLDAGTPPVYLGFGSLRAPADAAKVAIEAIRAHGRRVILSRGWADLV
FT                   LPDDRDDCFATGEVNQQVLFRRVAAVIHHGGAGTTHVATRAGAPQILVPQIADQPYYAG
FT                   RVAELGIGVAHDGPTPTFESLSAALTTALAPETRVRAEAVAGTVLTDGAAAAADLLFAA
FT                   VGEEKPAVPA"
XX
SQ   Sequence 1230 BP; 155 A; 442 C; 461 G; 172 T; 0 other;
     atgcgtgtgt tgttgtcgac gtgcggaagt cgcggggacg tcgaaccctt ggtggcgctg        60
     gcggtccggc tgcgggcgcg cggtgccgag gtgcggatgt gcgcgccgcc ggactgcgcg       120
     gaccggctgg ccgaggtcgg ggtgccgcat ctgcccctcg gccggtcggc gcgcccggcg       180
     gcggcggggg aggcgaagcc cttgacggcc gaggacatgc tccggttcac gaccgagacg       240
     atcgccatgc agttcgaccg gatcccggcg gccgccgagg ggtgcgacgc ggtggtgacg       300
     accgggctgc tggccgccgc gctcggcgtc cggtcggtgg ccgagaagct ggggatcccc       360
     tacttctacg ccttccattg cccgagctac gtgccgtcgc cgtactacgc gccgccgccg       420
     ccactcggcg agccgcccgc gccggagggg accgacatcc gggcgttgtg ggagcggaac       480
     aatcagagcg ccttccgccg gtacggggcc ccgctcaaga gccaacgggc cgcgatcggc       540
     cttccgccgg tggaagacat cttcgaacac ggctacaccg atcacccgtg gatggcggcg       600
     gaccaggtcc tggccccgct gcagccgacg gatctcgacg cggtgcagac cggcgcgtgg       660
     atcctccccg acgagcggcc gctttccccg gagatggaag cgttcctgga cgccgggaca       720
     ccgccggtct acctgggatt cggcagcctg cgtgcccccg ccgacgccgc gaaggtggcc       780
     atcgaggcga tccgggccca cggccgccgc gtgatcctct cccgtggctg ggccgatctg       840
     gtcctgcccg acgaccgtga cgactgtttc gccaccggtg aggtgaacca gcaggtgctg       900
     ttccgccggg tggccgccgt catccaccac ggtggcgcgg ggacgacgca tgtggccacg       960
     cgggcgggcg caccccagat cctggtgccc cagatcgcgg accagccgta ctacgccggc      1020
     cgggtggccg aactcgggat cggcgtcgcg catgacggcc cgacgccgac cttcgagtcc      1080
     ctttcggccg cgctcaccac ggccctcgcg ccggaaacgc gggtgcgggc ggaggccgtg      1140
     gcgggcaccg tcctcaccga cggggcggcg gctgccgcgg atctgttgtt cgccgcggtc      1200
     ggcgaggaga agcccgccgt tcccgcctga                                       1230
//