EBI Dbfetch

ID   AF351621; SV 1; linear; genomic DNA; STD; PRO; 1227 BP.
XX
AC   AF351621;
XX
DT   11-APR-2001 (Rel. 67, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   Amycolatopsis orientalis glycosyltransferase GtfD (gtfD) gene, complete
DE   cds.
XX
KW   .
XX
OS   Amycolatopsis orientalis
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Pseudonocardineae; Pseudonocardiaceae; Amycolatopsis.
XX
RN   [1]
RP   1-1227
RX   DOI; 10.1021/bi010050w
RX   PUBMED; 11294642.
RA   Losey H.C., Peczuh M.W., Chen Z., Eggert U.S., Dong S.D., Pelczer I.,
RA   Kahne D., Walsh C.T.;
RT   "Tandem action of glycosyltransferases in the maturation of vancomycin and
RT   teicoplanin aglycones: novel glycopeptides";
RL   Biochemistry 40(15):4745-4755(2001).
XX
RN   [2]
RP   1-1227
RA   Losey H.C., Walsh C.T.;
RT   ;
RL   Submitted (21-FEB-2001) to the EMBL/GenBank/DDBJ databases.
RL   Biological Chemistry and Molecular Pharmacology, Harvard Medical School,
RL   240 Longwood Ave., Boston, MA 02115, USA
XX
DR   StrainInfo; 119664; ATCC 19795.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1227
FT                   /organism="Amycolatopsis orientalis"
FT                   /strain="ATCC19795"
FT                   /mol_type="genomic DNA"
FT                   /note="vancomycin producer"
FT                   /db_xref="taxon:31958"
FT   CDS             1..1227
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="gtfD"
FT                   /product="glycosyltransferase GtfD"
FT                   /db_xref="GOA:Q9AFC7"
FT                   /db_xref="InterPro:IPR002213"
FT                   /db_xref="InterPro:IPR004276"
FT                   /db_xref="PDB:1RRV"
FT                   /db_xref="UniProtKB/TrEMBL:Q9AFC7"
FT                   /protein_id="AAK31352.1"
FT                   /translation="MRVLLSVCGTRGDVEIGVALADRLKALGVQTRMCAPPAAEERLAE
FT                   VGVPHVPVGLPQHMMLQEGMPPPPPEEEQRLAAMTVEMQFDAVPGAAEGCAAVVAVGDL
FT                   AAATGVRSVAEKLGLPFFYSVPSPVYLASPHLPPAYDEPTTPGVTDIRVLWEERAARFA
FT                   DRYGPTLNRRRAEIGLPPVEDVFGYGHGERPCWPADPVLAPLQPDVDAVQTGAWLLSDE
FT                   RPLPPELEAFLAAGSPPVHIGFGSSSGRGIADAAKVAVEAIRAQGRRVILSRGWTELVL
FT                   PDDRDDCFAIDEVNFQALFRRVAAVIHHGSAGTEHVATRAGVPQLVIPRNTDQPYFAGR
FT                   VAALGIGVAHDGPTPTFESLSAALTTVLAPETRARAEAVAGMVLTDGAAAAADLVLAAV
FT                   GREKPAVPA"
XX
SQ   Sequence 1227 BP; 145 A; 429 C; 476 G; 177 T; 0 other;
     atgcgtgtgt tgttgtcggt gtgcggaacc cgcggggacg tcgaaatcgg ggtggcgctg        60
     gcggaccgat tgaaggcgct cggcgtccag acacggatgt gcgcgccgcc cgccgccgag       120
     gagcggctgg cggaggtcgg ggtgccgcat gtgccggtcg gcctgccgca gcacatgatg       180
     ttgcaggagg gcatgccgcc gccaccgccc gaagaggaac agcggctggc ggccatgacg       240
     gtcgagatgc agttcgacgc cgtccccggg gccgccgaag ggtgtgccgc ggtggtggcg       300
     gtcggcgatc tggccgccgc gaccggtgtc cggtcggtgg ccgagaaact gggcctgccc       360
     ttcttctatt ccgtccccag tccggtctat ctggcgtccc cgcaccttcc gccggcttac       420
     gacgagccga ccacgccggg cgtgaccgac atccgggtgc tgtgggagga gcgggccgcc       480
     cggttcgccg accggtacgg gcccacgctc aaccgtcgcc gggccgagat cggcctgccg       540
     ccggtggagg acgtcttcgg ctacggccac ggtgagcggc cctgctggcc ggcggacccg       600
     gtactggcgc ccctgcagcc ggacgtcgac gcggtgcaga ccggtgcgtg gctcctgtcc       660
     gacgagcggc cgcttccccc ggagctggaa gcgttcctgg ccgccgggtc accgccggtg       720
     cacatcggtt tcggcagctc gtccggccgc gggatcgccg acgccgcgaa ggtggccgtc       780
     gaggcgatcc gggcccaagg ccggcgggtg atcctctcgc gggggtggac cgagctggtc       840
     ctgcccgacg accgtgacga ctgtttcgcc atcgacgagg tgaacttcca ggcgctgttc       900
     cgccgggtgg ccgccgtcat ccaccacggc agcgcgggta cggagcacgt ggccacgcgg       960
     gccggcgtcc cccagctcgt gatcccccgg aacacggacc agccgtactt cgccgggcgg      1020
     gtggccgcgc tggggatcgg tgtggcgcat gacggcccga cgccgacctt cgagtccctt      1080
     tcggccgcgc tcaccacggt gctcgcgccg gagacgcggg cgcgggcgga ggccgtggcg      1140
     ggcatggtcc tcaccgacgg ggcggcggcg gccgcggatc tggtgctcgc cgcggtcggc      1200
     cgggagaagc ccgccgttcc cgcgtga                                          1227
//