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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AF343964; SV 1; linear; mRNA; STD; ROD; 1344 BP.
XX
AC   AF343964;
XX
DT   14-JUN-2001 (Rel. 68, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   Cricetulus griseus mutant N-acetylglucoaminyltransferase I (Mgat1) mRNA,
DE   complete cds.
XX
KW   .
XX
OS   Cricetulus griseus (Chinese hamster)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea;
OC   Cricetidae; Cricetinae; Cricetulus.
XX
RN   [1]
RP   1-1344
RX   DOI; 10.1021/bi015538b
RX   PUBMED; 11467936.
RA   Chen W., Unligil U.M., Rini J.M., Stanley P.;
RT   "Independent Lec1A CHO glycosylation mutants arise from point mutations in
RT   N-acetylglucosaminyltransferase I that reduce affinity for both substrates.
RT   Molecular consequences based on the crystal structure of GlcNAc-TI";
RL   Biochemistry 40(30):8765-8772(2001).
XX
RN   [2]
RP   1-1344
RA   Chen W.;
RT   ;
RL   Submitted (31-JAN-2001) to the EMBL/GenBank/DDBJ databases.
RL   Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave.,
RL   Bronx, NY 10461, USA
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1344
FT                   /organism="Cricetulus griseus"
FT                   /mol_type="mRNA"
FT                   /cell_line="Lec1A CHO mutant"
FT                   /db_xref="taxon:10029"
FT   CDS             1..1344
FT                   /codon_start=1
FT                   /gene="Mgat1"
FT                   /product="mutant N-acetylglucoaminyltransferase I"
FT                   /note="GlcNAc-TI"
FT                   /db_xref="GOA:Q924B9"
FT                   /db_xref="HSSP:1FOA"
FT                   /db_xref="InterPro:IPR004139"
FT                   /db_xref="UniProtKB/TrEMBL:Q924B9"
FT                   /protein_id="AAK61869.1"
FT                   /translation="MLKKQSAGLVLWGAILFVGWNALLLLFFWTRPAPGRPPSDSAIDD
FT                   DPASLTREVFRLAEDAEVELERQRGLLQQIREHHALWRQRWKVPTVAPPAWPRVPATPS
FT                   PAVIPILVIACDRSTVRRCLDKLLHYRPSAEHFPIIVSQDCGHEETAQVIASYGSAVTH
FT                   IRQPDLSNIAVPPDHRKFQGYYKIARHYRWALGQIFNKFKFPAAVVVENDLEVAPDFFE
FT                   YFQATYPLLRTDPSLWCVSAWNDNGKEQMVDSSKPELLYRTDFFPGLGWLLMAELWTEL
FT                   EPKWPKAFWDDWMRRPEQRKGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQF
FT                   VSFTQLDLSYLQREAYDRDFLARVYSAPLLQVEKVRTNDQKELGEVRVQYTSRDSFKAF
FT                   AKALGVMDDLKSGVPRAGYRGVVTFQFRGRRVHLAPPQTWEGYDPSWN"
XX
SQ   Sequence 1344 BP; 274 A; 375 C; 394 G; 301 T; 0 other;
     atgctgaaga agcagtctgc agggcttgtg ctttggggtg ctatcctctt tgtgggctgg        60
     aatgccctgc tgctcctctt cttctggaca cgcccagccc ctggcaggcc cccctcagat       120
     agtgctatcg atgatgaccc tgccagcctc acccgtgagg tgttccgcct ggctgaggac       180
     gctgaggtgg agttggagcg gcagcggggg ctgttgcagc aaatcaggga gcatcatgct       240
     ttgtggagac agaggtggaa agtgcccacc gtggcccctc cagcctggcc ccgtgtgcct       300
     gcgaccccct caccagccgt gatccccatc ctggtcattg cctgtgaccg cagcactgtc       360
     cggcgctgct tggataagtt gttgcactat cggccctcag ctgagcattt ccccatcatt       420
     gtcagccagg actgcgggca cgaagagaca gcacaggtca ttgcttccta tggcagtgca       480
     gtcacacaca tccggcagcc agacctgagt aacatcgctg tgcccccaga ccaccgcaag       540
     ttccagggtt actacaagat cgccaggcac taccgctggg cactgggcca gatcttcaac       600
     aagttcaagt tcccagcagc tgtggtagtg gagaacgatc tggaggtggc accagacttc       660
     tttgagtact tccaggccac ctacccactg ctgagaacag acccctccct ttggtgtgtg       720
     tctgcttgga atgacaatgg caaggagcag atggtagact caagcaaacc tgagctgctc       780
     tatcgaacag acttttttcc tggccttggc tggctgctga tggctgagct gtggacagag       840
     ctggagccca agtggcccaa ggccttctgg gatgactgga tgcgcagacc tgagcagcgg       900
     aaggggcggg cctgtattcg tccagaaatt tcaagaacga tgacctttgg ccgtaagggt       960
     gtgagccatg ggcagttctt tgatcagcat cttaagttca tcaagctgaa ccagcagttc      1020
     gtgtctttca cccagttgga tttgtcatac ttgcagcggg aggcttatga ccgggatttc      1080
     cttgcccgtg tctatagtgc ccccctgcta caggtggaga aagtgaggac caatgatcag      1140
     aaggagctgg gggaggtgcg ggtacagtac actagcagag acagcttcaa ggcctttgct      1200
     aaggccctgg gtgtcatgga tgacctcaag tctggtgtcc ccagagctgg ctaccggggc      1260
     gttgtcactt tccagttcag gggtcgacgt gtccacctgg cacccccaca aacctgggaa      1320
     ggctatgatc ctagctggaa ttag                                             1344
//
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