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EBI DbfetchID AF305580; SV 1; linear; genomic DNA; STD; PRO; 5139 BP. XX AC AF305580; XX DT 13-OCT-2000 (Rel. 65, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Methanosarcina acetivorans strain C2A proABC locus, complete sequence. XX KW . XX OS Methanosarcina acetivorans OC Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales; OC Methanosarcinaceae; Methanosarcina. XX RN [1] RP 1-5139 RX PUBMED; 11844777. RA Zhang J.K., White A.K., Kuettner H.C., Boccazzi P., Metcalf W.W.; RT "Directed mutagenesis and plasmid-based complementation in the methanogenic RT archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of RT proline biosynthesis"; RL J. Bacteriol. 184(5):1449-1454(2002). XX RN [2] RP 1-5139 RA Zhang J.K., White A.K., Kuettner H.C., Boccazzi P., Metcalf W.W.; RT ; RL Submitted (13-SEP-2000) to the EMBL/GenBank/DDBJ databases. RL Microbiology, University of Illinois, 601 S. Goodwin, Urbana, IL 61801, USA XX FH Key Location/Qualifiers FH FT source 1..5139 FT /organism="Methanosarcina acetivorans" FT /strain="C2A" FT /mol_type="genomic DNA" FT /db_xref="taxon:2214" FT CDS 909..2252 FT /codon_start=1 FT /transl_table=11 FT /gene="proA" FT /product="ProA" FT /function="involved in proline biosynthesis" FT /note="glutamate semialdehyde dehydrogenase" FT /db_xref="GOA:Q9HHA1" FT /db_xref="HSSP:1O20" FT /db_xref="InterPro:IPR012134" FT /db_xref="UniProtKB/Swiss-Prot:Q9HHA1" FT /protein_id="AAG22031.1" FT /translation="MAEDIETKVVKAKKASIELSSVSEEVKNRALEAMAESLDRERKAI FT LEANSKDLEYASELKKVGKLTQALVDRLKVTDSKIDGMIAGIMDVIKLKDPVGETLSTL FT ELDNDLILYQISCPIGLIGVIFESRPDVVPQVMSLCLKSGNATIFKGGSEARESNRTIF FT EILVKAIESTEGMPKGAFQLMETREEIMSLLSLDAYVDLLIPRGSNEFVKFIQDNTKIP FT VLGHTSGICHIYVDEFADPDTAWKVCFDAKVQYPAVCNAIETLLVNRKIAEAFLPKMAE FT MYIKAGVELRCDKDSYILLAEKGLSPLSKATEEDWSLEYNDLILSIKLVDTIKEAIAHI FT NTFGSHHTDGIITENASRRKEFIGLVDSSSVMVNASTRFADGYRYGKGAEVGISTNKIH FT SRGPVGMEGLLIYKYILMGKGQVVADYAGENAKPYTHRKLDLKFADVN" FT CDS 2391..3548 FT /codon_start=1 FT /transl_table=11 FT /gene="proB" FT /product="ProB" FT /function="involved in proline biosynthesis" FT /note="glutamate kinase" FT /db_xref="GOA:Q9HHA0" FT /db_xref="InterPro:IPR019797" FT /db_xref="UniProtKB/Swiss-Prot:Q9HHA0" FT /protein_id="AAG22032.1" FT /translation="MTDREQFFRDVNKIVIKIGTSSITRKGCDHTKENCNIDPAFMESI FT AAQVYELRKHGKEVILVSSGAIGVGLNELGIAPKPREIPIRQAAAAVGQSILMQDWSRA FT FSKYGMKVAQILLTYEFYSDRVTYLNLRNSISTLLEYEVVPIINENDCTCTNEIEAIFG FT DNDKLSAMVASKIDADLLIILSDIDGLFDRNPKTHSDAKLLTLIKKITPEIESYGGDPT FT NFKGVGGMRTKIKAAKICSMAGCYVVIANSEIEDVVTKILSGEEIGTLFLAERHIQKNR FT ARWIILARASGTVRVDAGAKAAVLGKNSLLPAGIVDIEGTFDRGDVVKLECEGKVFAKG FT ITNYTSKELIKIKGAQTNQIDNILGYNNYDNVIKKENIGILEGIN" FT CDS 3822..4634 FT /codon_start=1 FT /transl_table=11 FT /gene="proC" FT /product="ProC" FT /function="involved in proline biosynthesis" FT /note="1-pyrroline-5-carboxylate reductase" FT /db_xref="GOA:Q9HH99" FT /db_xref="InterPro:IPR000304" FT /db_xref="UniProtKB/Swiss-Prot:Q9HH99" FT /protein_id="AAG22033.1" FT /translation="MENQKIGFIGAGKMGSALMQGTIKAGIVTPENIGASDVYEPFLKD FT LQAKLGIRVSTDNAVIVRESDILILAVKPQTLSSVLSNLKNEITSEKLVISIAAGVPLS FT TYEDALLEGTRVVRVMPNIAATVSEAASGIAPGKNATPEDLKAALEIFSAVGTAVQVPE FT SLMDAVTGLSGSGPAFIFPVIEAMADGAVLEGMDRKSALTLAAQTVLGAAKMALETGMH FT PGELKDMVTSPAGTTIQGIHSLEEAGIRAAFMNAVIRASERSKELGKK" XX SQ Sequence 5139 BP; 1564 A; 1095 C; 1090 G; 1390 T; 0 other; gatcatgtaa tgctattttt actttatccg agtcgtctgc ccggactaac gcaaatgatt 60 tcatttttta cacaccacca atctacagaa gttatcccca tttcgatatg gatttacccc 120 tataataaac tttgtgtatg aaacggctta gtggacgacc ataaattccc agccgaatgc 180 agcaccctca ttagatcgag actgttaaaa attgccattt ccgtggcctt aatattttgt 240 ccgcctttcg ccttattgca aaatatgttc aaaaattaca ggggtatttc acaagctctt 300 ttttcaagat ttagtatgcg ctcccatgaa atagagtcgc atatgctata cttatatgca 360 tcgtatatat attcgttttg aaataatatt ttaccaacag gggaacttta cttaaaatcc 420 accttagttg agtttttaag agaagttttt tattggccaa gcaatataat attttattat 480 tagatttcgt aaacttcatt ttttgattcc cggattaagt atgaatggag ccctttctcc 540 aggttttttt tgaaataagg ggatatcaag actttaaatg aatatatata ttcacttaaa 600 atatcatttt acattaaata aaaatagtat aatataaact tactcatttg attctgccat 660 attgggcaaa aataacttcg ttaaatggga tcttttattt agaaccttgc catatcatat 720 accgagacag tatacaaaaa tatttatcat agaactctct gtgttctgtt ttcatctccg 780 tgcatgaaaa aacccaagta ttctatggac tgagttcttc ctcttataaa tctcaatcaa 840 ttacatctta taatctcaat tatattttat aatctcgatt agggtcatta cataaagacg 900 gtgaatatat ggctgaagat attgaaacta aagttgttaa ggcaaaaaaa gcttcaattg 960 agctttccag tgtaagcgaa gaggtaaaaa atagggcgct tgaggctatg gcagaatccc 1020 tggacagaga aagaaaagct attcttgaag caaactcaaa agaccttgaa tatgcatccg 1080 aactgaaaaa agtcgggaag cttacccagg cccttgtgga caggctcaag gtcacggact 1140 caaagattga cgggatgatc gcaggaatca tggatgtaat aaagctcaaa gatcctgttg 1200 gggagactct ttccacactc gagcttgaca acgatctgat cctctatcag attagctgcc 1260 ccataggcct gataggagtg atttttgaat cccggcctga cgttgtgcct caggtaatgt 1320 ccctctgtct gaagagcgga aacgcaacca ttttcaaagg cggaagcgaa gccagagagt 1380 cgaaccgcac aattttcgaa attcttgtca aagccataga atccaccgag ggaatgccga 1440 aaggagcctt ccagctaatg gaaacaagag aagagatcat gagccttctg agccttgatg 1500 cgtatgtcga cctcctgatc cccagaggct ctaatgagtt tgtcaagttc attcaggaca 1560 atacaaaaat cccggtactc gggcacacaa gcgggatttg ccatatctat gtagatgagt 1620 ttgcagaccc ggatactgcc tggaaagttt gtttcgatgc caaggtccag taccctgcag 1680 tatgcaacgc cattgaaacc ctgctggtaa accgcaaaat tgcggaagct ttcctgccga 1740 aaatggcaga gatgtacata aaagcaggag tggaactgcg ctgtgacaaa gatagctata 1800 tcctgctcgc agaaaaaggg ctctctcccc tctcaaaggc tacagaagag gactggagcc 1860 tcgaatataa cgaccttatc ctttcgataa aactcgtaga tactataaaa gaagcaattg 1920 cccacataaa caccttcggc tcccaccaca cagacgggat aataacggaa aatgcttctc 1980 ggagaaaaga attcataggg ctagttgact cctcaagcgt aatggtaaat gcctctacac 2040 ggtttgcgga cggctaccgc tacggaaaag gtgccgaagt agggatcagt acgaataaga 2100 tccattcccg tggacctgtt ggcatggaag ggctgctgat ttacaagtat atccttatgg 2160 gtaaagggca agtcgttgcc gactatgcag gagaaaacgc aaaaccctat actcacagga 2220 agcttgacct taagtttgcg gatgtgaatt aacacatccc tgaatattta tgatatgttt 2280 atatacttga attgaaatac cagcgcaggt taagtgcaaa aactggaaat cctggctgcg 2340 atcgagatta cttgcagcca tcttccaaat aaccttcaga ggcacttcca ttgacagata 2400 gagaacaatt cttccgtgat gttaataaaa tcgttattaa gattggaact tcttcgataa 2460 ccagaaaggg ttgcgaccac actaaagaaa actgcaatat tgaccctgcc tttatggaaa 2520 gcatcgcagc tcaggtctac gaacttcgaa aacatggaaa agaagtaatt cttgtaagtt 2580 cgggagcaat aggcgtgggg cttaacgagc tgggcatagc ccccaaaccc cgcgagatcc 2640 ccattaggca ggcagccgca gctgttgggc agagcatact gatgcaggac tggagcaggg 2700 ctttttccaa atacgggatg aaggtagccc agattcttct tacctatgaa ttctattccg 2760 atcgcgtaac ctaccttaac ctgaggaaca gcatttcaac ccttctggag tatgaagtcg 2820 tcccgataat aaacgaaaac gactgtacat gtacaaacga gattgaagca atcttcgggg 2880 acaatgacaa gctctctgca atggttgcaa gcaaaattga tgctgacctc ctgatcatcc 2940 tttcagacat tgacgggctc tttgacagga acccaaagac gcacagtgat gcaaaactgc 3000 tgacccttat aaaaaagatc acacctgaaa tcgagagcta cgggggagac cccacaaact 3060 tcaagggtgt gggggggatg cgaaccaaga taaaagctgc aaaaatctgc agcatggcag 3120 gctgctatgt cgtaatcgca aacagtgaaa tagaggatgt ggttactaag atcctttccg 3180 gagaagagat cggaacgctc ttccttgcag agaggcatat tcagaaaaac cgcgctcgct 3240 ggataattct tgccagagcc tcagggactg tccgcgtgga cgccggagca aaagctgcgg 3300 tgctgggaaa aaacagcctc cttccggcag gtattgtgga tattgaggga acttttgaca 3360 ggggagacgt cgtgaagctc gaatgtgaag gcaaggtctt tgcaaaggga atcacgaact 3420 acacttccaa agaactgatt aaaatcaaag gagcccagac aaatcaaatc gacaatattc 3480 tgggctataa taattatgat aatgtaatta agaaagaaaa tatcggtata ctggaaggga 3540 taaactaaat tcatcgggct caggaaaatt ttccgaatct cgtctgatcc cggaaaacag 3600 taatctatcc agagagttca accgggctat tgagtttccg aaactttaac aggtaatatt 3660 gagtttccaa ctctaacagg atatgaattt tcgaaacctt aacaggtaat aaatgcagtt 3720 gtgcgttgaa attagcattc cgcagatgag tttcaaaaat agtccttttc aaatttacag 3780 ttaatataca atcattttct ggaaataagg agatttcgta catggaaaat caaaaaatag 3840 gatttatagg ggcaggaaaa atgggctctg ctctcatgca gggcaccata aaagcaggaa 3900 tcgtaacacc tgaaaatata ggtgcaagtg atgtatacga gccctttttg aaagacctgc 3960 aggcaaaact gggaatccgg gtgtcaaccg acaacgctgt tatcgtccga gaatcggaca 4020 ttcttatcct tgcagtaaag ccccagaccc tgagttcggt gctttcaaat ctgaagaatg 4080 aaatcacttc ggaaaaactc gtgatctcaa ttgctgcagg agtgcccctt tccacatacg 4140 aggacgctct ccttgaaggt accagggttg tgcgtgtaat gccaaacata gcagccacag 4200 tttctgaagc tgcttcaggg attgctcccg gaaagaatgc aactcctgag gacctgaaag 4260 ctgccctcga gatcttttct gcagtcggca cagcagtcca ggtgccggag tccctgatgg 4320 atgccgttac agggctttcg ggcagtggac ctgcgtttat tttcccggtt attgaagcaa 4380 tggctgacgg ggctgtcctt gaagggatgg ataggaaaag cgccctcact cttgcagccc 4440 agactgtgct cggagccgca aaaatggcgc ttgaaacagg catgcacccc ggagaactca 4500 aagatatggt cacatctccc gccggaacta ccatccaggg gattcactcc cttgaagaag 4560 ccggaatccg ggctgccttt atgaatgcag taataagagc aagtgaacgc tcaaaggaac 4620 tcggaaagaa ataaatccga ttccttctct ttacttttct atctctttac ttttctatca 4680 ttcatccttc ttttttaatt cggcatcttc atcagaaaat ttcgaaacgt aaattcattc 4740 agataaaatt tggttttaag gcttcgtttt tgccttttga ctttaccctg cctttgactt 4800 tactctgcct ttgaccctgc tttcggcagg taacttgttt ttctcgtaat atttttactg 4860 atagcgtttt tgctagaaaa cacattattc ctgatactag acattattgc aaaaacggct 4920 cattgtttat atatgtcgtg agatttgggc catatgttaa ccagtttcga acccgacata 4980 tatgcacata atgtttgaaa aacgccatca ttataaatac ttaaaatcta atgtcgacct 5040 gccgaatata ggtttgactt tactctgcct ttgactttac tctgcctttg actttactct 5100 gcctttgact ttatcctgtt catctagttt aaggatcca 5139 // |
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