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EBI DbfetchID AF304372; SV 2; linear; mRNA; STD; PLN; 6266 BP. XX AC AF304372; XX DT 02-MAY-2001 (Rel. 67, Created) DT 08-OCT-2002 (Rel. 73, Last updated, Version 2) XX DE Nicotiana alata putative beta-1,3-glucan synthase (Gsl1) mRNA, complete DE cds. XX KW . XX OS Nicotiana alata (Persian tobacco) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; OC Nicotiana. XX RN [1] RP 1-6266 RX DOI; 10.1104/pp.125.4.2040 RX PUBMED; 11299383. RA Doblin M.S., De Melis L., Newbigin E., Bacic A., Read S.M.; RT "Pollen tubes of Nicotiana alata express two genes from different RT beta-glucan synthase families"; RL Plant Physiol. 125(4):2040-2052(2001). XX RN [2] RP 1-6266 RA Doblin M.S., De Melis L., Newbigin E., Bacic A., Read S.M.; RT ; RL Submitted (12-SEP-2000) to the EMBL/GenBank/DDBJ databases. RL Plant Biology, University of California, 1 Shields Ave, Davis, CA 95616, RL USA XX RN [3] RC Sequence update by submitter RP 1-6266 RA Newbigin E.; RT ; RL Submitted (04-OCT-2002) to the EMBL/GenBank/DDBJ databases. RL Botany, University of Melbourne, VIC 3010, Australia XX DR RFAM; RF00315; snoJ33. XX CC On Oct 4, 2002 this sequence version replaced gi:13925875. XX FH Key Location/Qualifiers FH FT source 1..6266 FT /organism="Nicotiana alata" FT /mol_type="mRNA" FT /tissue_type="in-vitro grown pollen tubes" FT /note="genotype: S2S2" FT /db_xref="taxon:4087" FT CDS 220..6015 FT /codon_start=1 FT /gene="Gsl1" FT /product="putative beta-1,3-glucan synthase" FT /db_xref="GOA:Q93XQ3" FT /db_xref="InterPro:IPR003440" FT /db_xref="UniProtKB/TrEMBL:Q93XQ3" FT /protein_id="AAK49452.2" FT /translation="MTNPARPAEGMDPQTPGAGGAGPSSLTRRPSRSAATTTFSMEVFD FT NEVVPSSLQSIAPILRVAREIQNERPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTNL FT FQRLERDNASSLASRVKKTDAREIESFYKQYYEQYVVSLNKGEQADRAQLGKAYQTAGV FT LFEVLCAVNKSEKVEEVAPEIIAAANDVQAKKEIYAPYNILPLDSAGASQSIMQLEEVK FT AAVSALSNTRGLNWPASFEQQRQKAGELDVLDWLRAMFGFQRDNVRNQRENLILLLANI FT HIRLIPKAEPLNKLDDRAVDALMNKLFKNYKTWCKYLGKKHSLRLPQAPQEAQQRKILY FT MGLYLLIWGEAANIRFMPECLCYIFHNMAYELHGLLAGNVSIVTGENIKPSYGGDDESF FT LRKVITPIYRVIDKEAKKSKNGKAPYSTWCNYDDLNEFFWSQDCFSLGWPMRDDGDFFK FT STRDTTQGKGASTKKPGKMGKSYFVETRSFWHIFRSFDRLWTFFLLALQAMVIFAWSDI FT SVLDIFRKDSLYNLSSIFITAAFLRFLQSILDLVLNFPGYHRWKFTDVLRNVLKIIVSL FT AWSIILPLFYVQESNSELFTKIRNSLTFLDKMKGIPPLYLMAVAVYLLPNLLTAALFIF FT PMLRRWIENSDWLVVRFLLWWSQPRIYVGRGMHESQFALIKYTLFWVLLLCAKFAFSYF FT IQIKPLIKPTKMIMDINRVQYAWHEFFPDARSNYGAVLSLWAPVILVYFMDAQIWYAIF FT STLCGGVIGAFDRLGEIRTLDMLRSRFQSLPGAFNSYLVPSDKTDKKGFSLSKSFNEVS FT PSKRSEAAKFAQLWNEFICSFREEDLISDREMDLLLVPYSSDPSLKVIQWPPFLLASKI FT PIALDMASQFRSRDADLWKRICADEYMKCAVIECYESFKLVLNALVVGETEKRIIGIII FT KEVENNISKSTFLANFRTGPLQNPCTKFVDLLEILRDGDPSKRNNVVIALQDMLEIVTR FT DMMVNEIGELVELGHNGRDSGKQLFANTDSRTAIAFPPPVTAQWEEQIRRLYLLLTVRE FT SAVEVPTNLEARRRIHFFTNSLFMEMPRAPRVRKMLSFSVMTPYYSEETVYSKGDLEME FT NEDGVSIIYYLQKIYPDEWNNFMERLGCKKESEVWENDENILQLRHWASLRGQTLCRTV FT RGMMYYRRALKLQAFLDMASEGEILEGYKAVTVPSEEDKKSQRSLYAQLEAVADMKFTY FT VATCQNYGNQKRNGDRRATDILNLMVNNPSLRVAYIDEVEEREGGKAQKVYYSVLVKAV FT DNLDQEIYRIKLPGAAKIGEGKPENQNHAIIFSRGEALQTIDMNQDNYLEEALKMRNLL FT EEFNEDHGVRPPTILGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLARPLKVRFHYG FT HPDVFDRIFHITRGGISKSSRGINLSEDIFAGFNSTLRRGNITHHEYIQVGKGRDVGLN FT QISLFEAKVACGNGEQTLSRDVYRLGHRFDFFRMLSCYFTTTGFYISSMLVVLTVYAFL FT YGKLYLALSGLEQSIVKVARSKGDDALKAAMASQSVVQLGLLMALPMVMEIGLERGFRT FT AAGDIIIMNLQLAAVFFTFSLGTKLHYFGRTILHGGAKYRATGRGFVVRHEKFAENYRM FT YSRSHFTKALEILILLVAYQIYGTAVTDSVAFLLLSGSMWFLVVSWLFAPFLFNPSGFE FT WQKIVDDWEDWAKWISNHGGIGVPATKSWESWWDEEQEHLQYSGLIGRFCEILLSLRFL FT LFQYGIVYQLNVANNDKGIIVYGLSWLVIVFVMVVLKIVSMGRKKFSADFQLMFRLLKL FT FLFIGFIVTLVVLFKFLSLTVGDIFASLLAFLPTGWALLQIAQACRPVVKGIGMWGSVK FT ALARGYEYLMGLVIFAPVAVLAWFPFVSEFQTRLLFNQAFSRGLQIQRILAGGKKHK" XX SQ Sequence 6266 BP; 1725 A; 1256 C; 1447 G; 1838 T; 0 other; tgatcccgac acttctctct ctttcttttt ctttttgcct ccgcttgcca gaggctgctt 60 cttatcttca ttgtcaagct tcaaattgaa cggaagagag ggagaaagga aaagcggagc 120 tggaggagtt ggaagggagc ggtggtgtcg aagcctaagc tacattcagt tagctaagct 180 accgacaaca ctgacttagg agagaaaaaa aaccaaaaaa tgacgaaccc ggctagaccg 240 gcggagggaa tggatcccca aacaccggga gcgggtggag cggggccgtc gtcactgact 300 cgacggccgt cacgcagcgc cgccaccact accttctcta tggaggtgtt tgacaacgag 360 gttgttccct cttctctcca gtccattgcc ccaatccttc gcgtcgctag agaaatccaa 420 aatgaacgcc ctcgtgtcgc ctatctatgt cggttctatg cgtttgagaa ggcgcataga 480 ttagacccca cctcaagtgg acgtggtgtt cggcagttca aaaccaatct cttccaacgt 540 ttagagcgtg acaatgcatc gagtcttgct tctcgggtga aaaagactga tgcacgggag 600 attgagagct tttacaagca atactatgag caatatgtcg tgtctcttaa caagggagaa 660 caagcagata gagctcagct tggcaaagca tatcagactg ctggagtgct ttttgaagtt 720 ctttgtgctg ttaacaagtc tgaaaaagtt gaagaagttg ctcctgagat catcgccgca 780 gcaaatgacg ttcaggccaa aaaggaaata tatgccccct ataatattct tcccctggat 840 tcagctggag cgtcccagtc tattatgcaa cttgaagagg ttaaagcggc agtttctgct 900 ctctcgaata cacgtggctt gaattggcca gcttcatttg aacaacaaag gcaaaaagca 960 ggggaactgg atgttctaga ctggctaagg gccatgttcg ggtttcagag ggataatgta 1020 agaaaccagc gggagaatct gattttgctt cttgcaaata ttcatataag gctaattccc 1080 aaggctgagc cacttaacaa gcttgacgat cgagctgtgg atgcactaat gaacaagttg 1140 ttcaaaaact acaaaacatg gtgtaaatac ttgggaaaaa aacacagttt acggcttcct 1200 caagccccac aagaagcgca acaaagaaaa atactttaca tgggccttta ccttctaatc 1260 tggggtgaag cagctaatat tcgctttatg ccggagtgtc tatgttacat cttccataat 1320 atggcatacg aactccatgg cctcttagct ggaaatgtca gcattgtcac tggagaaaat 1380 atcaagcctt cttacggtgg agatgatgaa tctttcctac gaaaggtcat tactcccatt 1440 tatcgagtga tcgataagga agccaagaaa agcaaaaatg gaaaagcacc atactcaaca 1500 tggtgtaatt atgatgacct caatgaattt ttctggtcgc aagattgctt ttctttaggt 1560 tggcctatgc gtgatgacgg tgatttcttc aaatcaacac gtgacaccac acaaggaaaa 1620 ggagcttcta ctaaaaagcc tggaaaaatg gggaagtcct attttgttga gacccgatca 1680 ttttggcata tcttccgaag ctttgaccgt ctttggacct tctttctcct ggctttgcag 1740 gccatggtca tatttgcatg gagtgatatc tctgtgttgg atatttttcg gaaggattct 1800 ttatacaact tgtccagtat tttcatcaca gcagcgtttc ttcgctttct ccaaagcatc 1860 ttggaccttg ttctgaactt tccgggctat catagatgga agttcacaga tgtactgagg 1920 aatgttctga agataattgt cagccttgca tggtcgatca tcctcccact gttttatgtc 1980 caagaaagca actcagagct atttacgaag attcgaaatt ccctgacatt tctagacaag 2040 atgaagggca ttcctccttt gtacctaatg gctgtggctg tatacctact acccaattta 2100 ctcactgcag ctttgtttat attcccaatg ctccgtcggt ggattgagaa ctcggattgg 2160 cttgttgtaa ggtttttact gtggtggtca cagccaagaa tttatgttgg gaggggaatg 2220 catgaaagtc aatttgcact aataaagtat actcttttct gggtgctact tctttgtgca 2280 aaatttgcct tcagctattt tattcagata aaacctctta taaaacccac gaaaatgata 2340 atggacatca atcgcgttca gtatgcttgg catgagttct tccctgatgc taggagcaac 2400 tatggggctg tgctttccct gtgggcacca gttatcttgg tttactttat ggatgctcaa 2460 atttggtatg ctattttctc aactttgtgt ggtggtgtta taggggcctt tgatcgtctt 2520 ggtgagatac gaactctaga catgttaagg tcaagatttc agtctctgcc cggtgcattc 2580 aactcttact tggtgccttc tgacaaaact gacaaaaagg gattttctct ctccaagagt 2640 tttaatgagg tttcacccag taagagaagt gaagctgcaa aattcgctca gctttggaat 2700 gaatttatat gcagtttccg tgaagaagat cttataagtg atagggagat ggacctatta 2760 cttgttcctt attcctcgga ccccagtctg aaagttattc aatggccacc ctttttgctt 2820 gctagcaaga tcccaattgc attggacatg gcttcacaat ttcgttctag agatgctgac 2880 ctttggaagc gcatatgtgc ggatgaatac atgaagtgtg ctgtcattga atgttatgaa 2940 tcttttaaac ttgttcttaa tgccctggtt gttggagaaa ctgagaaaag gatcatagga 3000 atcatcataa aagaagttga aaacaacatt tcaaagagca cattccttgc aaatttcaga 3060 acaggtcctt tgcaaaaccc ttgcacgaaa tttgttgacc tacttgagat actgagagat 3120 ggagatccat ctaaaaggaa caacgttgtc atagcgctgc aggatatgtt agaaatagtg 3180 acccgagaca tgatggtgaa cgaaatcggg gaactagtag aacttggtca taatggaagg 3240 gattcaggga aacaactctt tgctaatact gactcgagaa cagctattgc attccctcct 3300 ccggtgacag cacagtggga agaacagatt cggcgtcttt atcttctctt aactgtaaga 3360 gaatcagcag ttgaagttcc aacaaacctt gaggcacgca gaaggattca ctttttcaca 3420 aattcattgt tcatggaaat gccgcgtgca cctcgagtgc gcaaaatgct ttcattcagt 3480 gtcatgacgc catactacag tgaggaaact gtctactcaa agggtgatct tgagatggaa 3540 aatgaagacg gtgtatcaat catttactac ctccaaaaga tttatccaga tgaatggaat 3600 aacttcatgg aacgtcttgg gtgtaaaaag gaatcagagg tctgggaaaa tgatgaaaac 3660 atcttgcagc tacgtcattg ggcctcctta aggggacaga ctctctgcag aacagttaga 3720 ggaatgatgt actacaggag agctttgaag cttcaagcat ttctcgacat ggcttcagaa 3780 ggagagattc ttgaaggata caaagctgtt acagtaccat ctgaggagga taagaaaagt 3840 caaagatcat tgtatgctca attagaagct gtggctgaca tgaaattcac ttatgtcgct 3900 acctgccaga attatggaaa tcagaagcga aatggagacc ggcgtgcaac tgatattctg 3960 aacttgatgg tcaacaaccc ctctctccgt gtagcatata ttgatgaagt cgaagaacgg 4020 gaaggtggaa aagctcagaa ggtttactat tcagtgttgg tgaaagcagt tgataatctt 4080 gatcaggaaa tataccgtat aaagttgcct ggtgcagcaa agataggaga aggaaagcct 4140 gaaaatcaga atcatgcaat tatcttctct cgtggggaag ctcttcaaac aattgacatg 4200 aatcaggaca attacttgga ggaagcttta aagatgcgta accttcttga ggagtttaac 4260 gaagatcacg gtgtgcgtcc acctacaata ttgggtgttc gtgagcatat cttcactgga 4320 agtgtttctt ctttggcttg gtttatgtct aatcaagaga caagttttgt caccattggt 4380 cagagagttc ttgcaaggcc cttgaaggtc cgcttccact atggacatcc agatgtattt 4440 gacagaattt tccacatcac tcgcggtggc attagcaaat cttcaagggg tatcaatctt 4500 agtgaggaca tatttgcagg cttcaactca acattgcgac gtggaaatat tactcaccat 4560 gaatacattc aagttggaaa gggacgagat gttggtctta accaaatctc tctctttgag 4620 gcaaaagtgg catgtggtaa tggagagcag acccttagcc gtgatgtgta cagattaggg 4680 catcgctttg actttttccg tatgctgtcc tgttatttca caactactgg attttatatc 4740 agctcaatgc tggtagtcct cacagtttat gcatttttat atggtaaact ctaccttgca 4800 ttgagtggac tggaacagtc cattgtcaaa gttgcaagat ccaagggtga tgatgctctc 4860 aaagctgcaa tggcctcaca atctgttgtt caacttggtt tattaatggc cctgccaatg 4920 gttatggaga ttgggctgga aagaggcttc agaactgctg caggtgatat catcatcatg 4980 aacctgcagt tggctgctgt cttctttaca ttctctcttg gaacaaagct ccattacttt 5040 ggccgcacaa ttttacatgg tggagcgaaa tacagagcaa ctgggcgtgg ttttgtcgtg 5100 agacacgaaa agtttgctga gaactacaga atgtactcaa ggagccattt caccaaagca 5160 ctggaaattt tgattttgct cgtagcttat caaatttatg gcacagctgt tactgattct 5220 gtagctttct tgcttctcag tggctcaatg tggttccttg tggtttcatg gctgtttgct 5280 cctttcctct ttaatccttc aggctttgaa tggcaaaaga tagtggatga ttgggaggat 5340 tgggccaagt ggatcagcaa tcatggaggt attggtgttc cggcaaccaa gagttgggag 5400 tcttggtggg atgaggaaca agagcatctg cagtattctg gtttgattgg ccgcttctgt 5460 gaaattcttc tttctctgcg cttcttactt ttccaatatg gaattgttta tcagctaaat 5520 gtggccaata atgataaggg tatcattgtt tatggtttgt cctggttggt cattgtgttc 5580 gtcatggtcg tcttgaagat cgtgtccatg ggtaggaaga agttcagtgc tgatttccag 5640 ctgatgttca ggcttctgaa attgttcctg ttcattggct tcatagtcac acttgtcgtg 5700 ctgttcaaat tcctaagtct tactgttgga gacatttttg ccagcttatt ggccttcttg 5760 ccaactggat gggctcttct tcagatagca caggcatgta ggccagtagt gaagggtata 5820 ggaatgtggg gatctgtgaa ggctctagcc agaggatatg agtacctaat ggggctcgtg 5880 atctttgcac ctgtggctgt tcttgcttgg ttcccatttg tctctgagtt ccagacaagg 5940 ctgcttttca accaggcttt cagcagaggg cttcagattc aacgtattct tgctggtggg 6000 aagaagcaca agtgagctta attgtcaatc acacaccaaa cgttctgtat ctttggcaaa 6060 gcagaagtat gacttccaca ccattgttca aagttccaca tcaggccttc tgctccctgg 6120 ttcgggacac attgtagagc atattagcat attatacttt cagaatacta cagcacaaga 6180 tgtatactcc tttgttataa atgcaagtaa ttgctcatat atgcaatatt tttttggttt 6240 atattgaaaa aaaaaaaaaa aaaaaa 6266 // |
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