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EBI DbfetchID AF274045; SV 1; linear; genomic DNA; STD; PRO; 7599 BP. XX AC AF274045; XX DT 10-JUL-2000 (Rel. 64, Created) DT 11-AUG-2000 (Rel. 64, Last updated, Version 2) XX DE Rhodococcus sp. strain V49 ohp operon, complete sequence. XX KW . XX OS Rhodococcus sp. OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Nocardiaceae; Rhodococcus. XX RN [1] RP 1-7599 RX DOI; 10.1023/A:1001784702230 RX PUBMED; 10068799. RA Powell J.A.C., Archer J.A.C.; RT "Molecular characterisation of a Rhodococcus ohp operon"; RL Antonie Van Leeuwenhoek 74(1-3):175-188(1998). XX RN [2] RP 1-7599 RA Powell J.A.C., Archer J.A.C.; RT ; RL Submitted (01-JUN-2000) to the EMBL/GenBank/DDBJ databases. RL Genetics, University of Cambridge, Downing Street, Cambridge, Cambs CB2 RL 3EH, UK XX FH Key Location/Qualifiers FH FT source 1..7599 FT /organism="Rhodococcus sp." FT /strain="V49; ATCC 19070" FT /mol_type="genomic DNA" FT /db_xref="taxon:1831" FT CDS 295..1035 FT /codon_start=1 FT /transl_table=11 FT /gene="ohpR" FT /product="putative operon regulator" FT /note="similar to Bacillus subtilis GntR protein; OhpR" FT /db_xref="GOA:Q9KH23" FT /db_xref="InterPro:IPR011711" FT /db_xref="UniProtKB/TrEMBL:Q9KH23" FT /protein_id="AAF81822.1" FT /translation="MTTTDTGPKPGSEAAALLANVRTSGARLSSALYDILKNRLLEGRY FT AAGEKIVVESIRQEFGVSKQPVMDALRRLSSDKLVHIVPQVGCEVVSYAPREVEDFYTL FT FGGFEGTIAAVAASRRTEAQLLELDLISARVDALITSHDPVVRARGYRVHNREFHAAIH FT AMAHSRIMEETSQRMWDLSDFLINTTGITNPLSSALPDRQHDHHEITEAIRNRDAAAAR FT EAMERHIVGTIAVIRDESNAQLPS" FT misc_feature 1197..1250 FT /gene="ohp operon" FT /note="putative; operon promoter and repressor binding FT site" FT /note="binding site" FT CDS 1399..2805 FT /codon_start=1 FT /transl_table=11 FT /gene="ohpA" FT /product="putative 3-(2-hydroxyphenyl) propionic acid FT transporter" FT /note="similar to members of the major facilitator FT superfamily, MFS; OhpA" FT /db_xref="GOA:Q9KH22" FT /db_xref="InterPro:IPR018486" FT /db_xref="UniProtKB/TrEMBL:Q9KH22" FT /protein_id="AAF81823.1" FT /translation="MSTTPTSPTKTSPLRVAMASFIGTTVEYYDFFIYGTAAALVFPEL FT FFPDVSSAIGILLSFATFSVGFLARPLGGIVFGHFGDRVGRKQMLVISLVGMGSATVLM FT GLLPGYAQIGIAAPILLTLLRLVQGFAVGGEWGGATLMAVEHAPTAKKGFFGSFSQMGA FT PAGTSVATLAFFAVSQLPDEQFLSWGWRLPFLFSAVLIVIGLFIRLSLAESPDFAEVKA FT QSAVVRMPIAEAFRKHWKEILLIAGTYLSQGVFAYICMAYLVSYGTTVAGISRTFALAG FT VFVAGIVAVLLYLVFGALSDTFGRKTMYLLGAAAMGVVIAPAFALINTGNPWLFMAAQV FT LVFGIAMAPAAGVTGSLFTMVFDADVRYSGVSIGYTISQVAGSAFAPTIATALYASTNT FT SNSIVTYLLIVSAISIVSVILLPGGWGRKGAASQLTRDQATSTPKMPDTETFSTRTVPD FT TAASLRVLDK" FT CDS 2807..4720 FT /codon_start=1 FT /transl_table=11 FT /gene="ohpB" FT /product="3-(2-hydroxyphenyl) propionic acid monooxygenase" FT /note="similar to members of the single-component FT flavin-dependent aromatic ring monooxygenase family; OhpB" FT /db_xref="GOA:Q9KH21" FT /db_xref="InterPro:IPR003042" FT /db_xref="UniProtKB/TrEMBL:Q9KH21" FT /protein_id="AAF81824.1" FT /translation="MMTDMSDHDRTSYDTDVVIVGLGPAGGTAALALASYGIRVHAVSM FT FPWVANSPRAHITNQRAVEVLRDLGVEDEARNYATPWDQMGDTLFTTSLAGEEIVRMQT FT WGTGDIRYGDYLSGSPCTMLDIPQPLMEPVLIKNAAERGAVISFNTEYLDHAQDEDGVT FT VRFRDVRSGTVFTQRARFLLGFDGARSKIAEQIGLPFEGELARAGTAYILFNADLSKYV FT AHRPSILHWIVNSKAGFGEIGMGLLRAIRPWDQWIAGWGFDMANGEPDVSDDVVLEQIR FT TLVGDPHLDVEIVSRSFWYVNRQWAEHYQSGRVFCGGDAVHRHPPSSGLGSNTSMQDAF FT NLAWKIAFVVKGYAGPGLLESYSPERVPVGKQIVARANQSRKDYAGLREWFDHESDDPV FT AAGLAKLKEPSSEGVALRERLYEALEVKNAEFNAQGVELNQRYTSSAVVPDPEAGEEVW FT VRDRELYLQATTRPGAKLPHAWLVGADGTRISTLDVTGKGMMTLLTGLGGQAWKRAAAK FT LDLPFLRTVVVGEPGTIDPYGYWRRVRDIDEAGALLVRPDGYVAWRHSAPVWDDTEALT FT SLENALTAVLDHSASDNGNPSGTNEPQYSTRAVPIVVPHVTAEDAAPASATRTTTVEGE FT NR" FT CDS 4717..5586 FT /codon_start=1 FT /transl_table=11 FT /gene="ohpC" FT /product="2-hydroxy-6-ketonona-2,4-dienedoic acid FT hydrolase" FT /note="similar to the family of hydrolases that includes FT XylF of Pseudomonas putida; OhpC" FT /db_xref="GOA:Q9KH20" FT /db_xref="InterPro:IPR000073" FT /db_xref="UniProtKB/TrEMBL:Q9KH20" FT /protein_id="AAF81825.1" FT /translation="MTRPYTSVWDDLNQVEFSQGFIQAGPYRTRYLHAGDSSKPTLILL FT HGITGHAEAYVRNLRSHSEHFNVWAIDFIGHGYSTKPDHPLEIKHYIDHVLQLLDAIGV FT EKASFSGESLGGWVTAQFAHDHPEKVDRIVLNTMGGTMANPQVMERLYTLSMEAAKDPS FT WERVKARLEWLMADPTMVTDDLIRTRQAIFQQPDWLKACEMNMALQDLETRKRNMITDA FT TLNGITVPAMVLWTTKDPSGPVDEAKRIASHIPGAKLAIMENCGHWPQYEDPETFNKLH FT LDFLLGRS" FT CDS 5721..6665 FT /codon_start=1 FT /transl_table=11 FT /gene="ohpD" FT /product="3-(2,3-dihydroxyphenyl) propionic acid FT dioxygenase" FT /note="similar to the extradiol aromatic ring-cleavage FT dioxygenase family that includes MhpB of Escherichia coli; FT OhpD" FT /db_xref="GOA:Q9KH19" FT /db_xref="InterPro:IPR004183" FT /db_xref="UniProtKB/Swiss-Prot:Q9KH19" FT /protein_id="AAF81826.1" FT /translation="MPVALCAMSHSPLMGRNDPEQEVIDAVDAAFDHARRFVADFAPDL FT IVIFAPDHYNGVFYDLLPPFCIGAAAQSVGDYGTEAGPLDVDRDAAYAVARDVLDSGID FT VAFSERMHVDHGFAQALQLLVGSITAVPTVPIFINSVAEPLGPVSRVRLLGEAVGRAAA FT KLDKRVLFVGSGGLSHDPPVPQFATAPEEVRERLIDGRNPSAAERDAREQRVITAGRDF FT AAGTAAIQPLNPEWDRHLLDVLASGDLEQIDAWTNDWFVEQAGHSSHEVRTWIAAYAAM FT SAAGKYRVTSTFYREIHEWIAGFGITTAVAVDE" FT misc_feature 6719..6814 FT /note="similar to 4-hydroxy-2-oxovalerate aldolase FT sequences" FT misc_feature 6817..7580 FT /note="similar to short chain dehydrogenase family FT sequences" XX SQ Sequence 7599 BP; 1332 A; 2743 C; 2288 G; 1236 T; 0 other; gaattccatg ttcttctcct tgcatgtggc ccgcgttgcc gagggcactg ctcggcctgt 60 cgcccgcaga gggcgcatgt ccgggtgcct ggatatggcg cgtacggcgt gccctccggc 120 gttaaccccg aggttggcca cgatgccccg gccatcaggt ctggaatgct agcgttccag 180 acgaaggtaa cccacagtga ctcacaccac aagtactaga atgcaagctg ttgcggtgag 240 cgccgcggca taagggggag ccatgtccgg gacgccgacg gaaagcctga ctcgatgacc 300 accaccgaca ccggccccaa gccgggcagt gaggccgccg ccctgctcgc caatgtccgc 360 acctcggggg cgcggctgtc ctccgcgttg tacgacattc tgaagaaccg gctgctcgaa 420 gggcgctatg cggcaggcga gaagatcgtc gtcgagtcga tccggcaaga gttcggggtg 480 agcaagcagc ccgtcatgga cgctctgcgc cgcctgtcca gcgacaagct ggtccacatc 540 gttccccagg tcggttgcga ggtcgtctcc tacgccccgc gcgaagtgga agacttctac 600 accctgttcg gcggtttcga agggaccatc gccgcggtag cggcctcccg gcggaccgag 660 gcccagttgc tggagctgga cctgatctcg gcgcgggtcg acgccctgat cacctcccac 720 gacccggtgg tccgcgcccg cgggtaccgc gtgcacaacc gggagttcca tgcggccatc 780 cacgcgatgg cgcactcgcg gatcatggag gagaccagcc agcgaatgtg ggatctgtcg 840 gacttcttga tcaacaccac cggcatcacc aacccgctct cgagcgcact gcccgaccgg 900 cagcatgacc accacgaaat caccgaggcc atccgcaacc gtgacgcagc tgccgcccgc 960 gaggccatgg aacgccacat cgtcggcacc atcgcagtaa tccgcgacga atccaacgcc 1020 cagctgccga gctagacccc gatacccggg ccatcgaccg gctccgctat cgcgccacct 1080 acgccgaggg gggactctcg gccgtagcgc tgcagacgat ccaccggcac cctccacgct 1140 gacccctgtc tcgccctaga gggccggcgc gccgtcgatc acctttaccc tcatccagag 1200 acttgcgtca ccctctatgc ccgagtagcg tctgaactag acgtctagca ttctagttga 1260 gtgctccctc tcgaagattc tccagagaac ccctctcgaa catccccaga agaaaggagc 1320 ggccatgacg accgcttcgc acgcatcgtc cttcggggca cgagcccact tccgcccaca 1380 gatcggggaa gcccgaccgt gagcaccaca cctacctccc cgacgaagac ctcaccgctg 1440 cgggtagcga tggccagctt catcggtacc accgtcgagt actacgactt cttcatctac 1500 ggcaccgcgg ccgcgctggt attccctgag ttgttcttcc cggatgtctc gtccgcgatc 1560 ggaatcctgt tgtcgttcgc gaccttcagc gttgggttcc tcgcccgccc gctgggtggc 1620 atagtgttcg ggcacttcgg tgaccgggtc ggccgcaagc agatgctggt gatctccctg 1680 gtcggaatgg gctcggccac cgtactgatg ggattgttgc ccggttacgc ccaaatcggg 1740 atcgccgccc ccatcctgct gaccctgctg cgcctggtgc agggctttgc cgtcggcggc 1800 gagtggggtg gagccaccct gatggccgtc gagcacgccc ccaccgcgaa gaagggcttt 1860 ttcggatcct tctcccagat gggggcaccc gccgggacca gcgtcgcaac cctggcgttc 1920 ttcgcggtct cccaattgcc cgacgagcag ttcctgagtt ggggctggcg actgccgttc 1980 ctgttcagcg cggtgctgat cgtgatcggg ctgttcattc gcctgtccct ggccgaaagc 2040 cccgacttcg ccgaggtgaa ggcacagagc gccgtggtgc gaatgccgat cgccgaagcg 2100 ttccgcaagc actggaagga aattctcctc atcgcgggca cctacctgtc ccaaggagtg 2160 ttcgcctata tctgcatggc ctacctcgtc tcctacggca ccaccgtcgc ggggatcagc 2220 cgcaccttcg ccctggccgg agtattcgtc gccggcatcg tcgccgtcct cctctacctc 2280 gtgttcggcg ctctgtccga cactttcggc cgcaagacca tgtacctgct cggcgccgcc 2340 gcgatgggtg tggtgatcgc ccccgccttc gcactgatca acaccggcaa cccgtggctg 2400 ttcatggccg cgcaggtgct ggtcttcgga attgcaatgg cccccgccgc cggcgtgaca 2460 ggctccctgt tcacgatggt cttcgacgcg gacgtgcgct acagcggtgt ctctatcggc 2520 tacaccatct cccaggtcgc cggctccgcg ttcgccccga cgatcgcgac cgccttgtac 2580 gcctccacca acaccagcaa ctcgatcgtg acctacctgc tgatcgtctc ggccatctcg 2640 atcgtctcgg tgatcctgct gcccggcggc tgggggcgca agggcgctgc gagccagctc 2700 actcgcgacc aggccacctc cacaccgaaa atgcctgaca ccgaaacatt ttcgactcgg 2760 acagttccgg acaccgcagc atccctgcgc gtcctcgaca agtgaagtga tgacagacat 2820 gagtgaccac gaccgcacct cctacgacac cgacgtcgtg atcgtcggcc tcggccccgc 2880 cggtggcaca gcggcgcttg ccctggccag ctacggcatc cgcgttcacg ccgtctcgat 2940 gttcccctgg gtggcgaact cgccgcgcgc gcacatcacc aaccagcgcg ccgtcgaagt 3000 gctgcgtgac ctgggcgtcg aagacgaggc gcgcaactac gccaccccgt gggaccagat 3060 gggcgacacg ctgttcacca cgagcctggc cggcgaggag atcgtccgga tgcagacctg 3120 gggtacgggc gatatccgct acggggacta cctgtccgga agcccctgca cgatgctcga 3180 cattccgcag cccctgatgg agccggtgct gatcaagaac gccgccgaac gtggtgcggt 3240 catcagcttc aacaccgaat acctcgacca cgcccaggac gaggacgggg tgaccgtccg 3300 gttccgcgac gtccgctcgg gcaccgtgtt cacccagcga gcccgcttcc tgctcggttt 3360 cgacggcgca cgatcgaaga tcgccgaaca gatcgggctt ccgttcgaag gtgaactcgc 3420 ccgcgccggt accgcgtaca tcctgttcaa cgcggacctg agcaaatatg tcgctcatcg 3480 gccgagcatc ttgcactgga tcgtcaactc gaaggccggt ttcggtgaga tcggcatggg 3540 tctgctgcgc gcgatccgac cgtgggacca gtggatcgcc ggctggggct tcgacatggc 3600 gaacggcgag ccggatgtct ccgacgacgt tgtcctcgaa cagatccgga ccctcgtcgg 3660 cgacccgcac ctggacgtcg agatcgtgtc gaggtccttc tggtacgtca accggcagtg 3720 ggctgagcac taccagtccg gtcgagtgtt ctgcggcggc gacgcggtgc accggcatcc 3780 gccgagcagc gggctgggct cgaacacgtc catgcaggac gcgttcaacc tggcatggaa 3840 gatcgcgttc gtcgtgaagg ggtatgcagg accgggtctg ctcgagtcct actctcctga 3900 gcgtgttccg gtcggcaaac agatcgtcgc tcgcgccaac cagtcccgca aggactacgc 3960 cgggctgcgc gaatggttcg atcacgagag cgacgacccg gtcgccgccg gcctggcaaa 4020 gttgaaggaa ccctcgtccg aaggtgttgc tctgcgtgag cggctgtacg aggcgctgga 4080 ggtgaagaac gccgaattca acgcccaggg cgtcgaactc aaccagcgct acacctcgtc 4140 cgcggtcgtt cccgaccccg aggcgggcga ggaagtgtgg gtgcgcgatc gtgagctgta 4200 cctgcaggcc accacccggc cgggcgcgaa gctgccgcat gcgtggctgg tcggcgccga 4260 cggaacccgc atctccaccc tcgacgtcac cggcaaggga atgatgaccc tgctgaccgg 4320 actcggcggc caggcatgga agcgtgccgc cgccaaactc gacctgccgt tcctgcggac 4380 cgtcgttgtc ggcgaacccg gcaccatcga cccttacgga tactggcggc gggtccgcga 4440 catcgacgag gccggcgccc tgctcgtgcg gcccgacggc tacgtcgcgt ggcgacacag 4500 tgctccggtc tgggacgaca ccgaagcgct caccagcctc gagaacgctc tcaccgcggt 4560 cctcgaccac tcggccagcg acaacgggaa cccgagcggc acaaacgagc cgcagtacag 4620 cacccgggcc gtgccgatcg tcgttccgca cgttaccgcc gaggatgcag caccagcttc 4680 cgccacccgc accaccacag tcgagggaga gaaccgatga cccgtcctta caccagcgtc 4740 tgggacgacc tgaaccaggt cgagttcagc cagggattca tccaggccgg cccctaccgg 4800 acccgatacc tgcacgccgg cgactcgtcc aagcccacgc tgatcctgct gcacggcatc 4860 accggccacg ccgaggcgta cgtgcgcaat ctgcgctcgc attccgagca cttcaacgtc 4920 tgggcaatcg acttcatcgg ccacggctat tcgaccaagc ccgaccaccc gctcgagatc 4980 aagcactaca tcgaccacgt gctgcagttg ctggacgcca tcggcgtcga gaaggcctcg 5040 ttttccgggg agtctctcgg cggttgggtc accgcccagt tcgcgcacga ccatcccgag 5100 aaggtcgacc ggatcgtgct caacaccatg ggcggcacca tggccaaccc tcaggtgatg 5160 gaacgtctct ataccctgtc gatggaagcg gcgaaggacc cgagctggga acgcgtcaaa 5220 gcacgcctcg aatggctcat ggccgacccg accatggtca ccgacgacct gatccgcacc 5280 cgccaggcca tcttccagca gccggattgg ctcaaggcct gcgagatgaa catggcactg 5340 caggacctcg aaacccgcaa gcggaacatg atcaccgacg ccactctcaa cggcatcacg 5400 gtgcccgcga tggtgctgtg gaccaccaag gacccctccg gtccggtcga cgaagccaag 5460 cgcatcgcct cccacatccc gggcgccaag ctggccatca tggagaactg tggccactgg 5520 ccccagtacg aggaccccga gaccttcaac aagctgcatc tggacttcct cctcggtcgc 5580 agctgacaca gaccccggcc ggtgccgcca acccctgcaa cccgggcggc accggccgga 5640 tctcacttac ccgacctatt gcgctctcgt ccggaccccc ggagagaaag cgccgaagca 5700 gcagcaagga gaccgccgcg atgcctgtag cgctgtgcgc gatgtcgcac tcccccctga 5760 tgggacgcaa cgaccccgaa caggaagtca tcgacgccgt cgacgccgca ttcgaccacg 5820 cgcgccggtt cgtcgccgac ttcgcccccg atctcatcgt catcttcgcc cccgaccact 5880 acaacggcgt cttctacgac ctgctgccgc cgttctgtat cggtgccgcc gcgcagtccg 5940 tcggcgacta cggcaccgaa gccggccctc tcgacgtcga ccgtgacgcc gcctacgcag 6000 tcgcccgcga cgtcctcgac agcggcatcg acgtcgcatt ctccgaacgc atgcacgtcg 6060 accacggatt cgcccaagca ctccaattgc tggtcggatc gatcaccgcc gtgccgaccg 6120 tgccgatctt catcaattcg gtcgccgaac cgctcggccc ggtcagccgg gtacggctgc 6180 tcggcgaggc ggtcgggcgg gccgctgcca agctggacaa gcgtgtgctg ttcgtcggat 6240 ccggcggcct gtcccacgac ccgccggtcc cgcagttcgc caccgcgcca gaggaagtgc 6300 gcgagcggtt gatcgacggc cgcaatccca gtgccgccga acgtgatgcc cgcgaacagc 6360 gcgtcatcac cgccgggcgg gacttcgccg ccggcaccgc cgccatccag ccactgaacc 6420 ccgaatggga ccggcacctg ctcgacgtcc tcgcctccgg cgacctcgag cagatcgacg 6480 cgtggaccaa cgactggttc gtcgaacagg ccggacactc ctcccacgaa gtgcgcacct 6540 ggatcgccgc gtacgcggca atgagcgccg ccgggaagta ccgcgtcacc tcgaccttct 6600 accgcgaaat ccacgagtgg atagcaggat tcgggattac taccgccgtc gccgtcgacg 6660 aatagacccc gccgctcccg ccccgcagtc ccaacgaagg gtggccccgg atgacctccg 6720 tccgcccgtg ctcgccgtcg gtgaacgcgg gctggtcggt gggcaggaag acctcatcgc 6780 cgacatcgcc ctcgacctcg cagctcgtca gtaggaatgc gcacgggccg acgagtcgcg 6840 ctggtcaccg gggccagccg cggcatcggg gcggccatcg cagatgcggt ggccgcctcc 6900 ggtgccgccg taatcgtcca ctacggatcc gatcggacgg ccgccgctgc ggtgtcgacg 6960 gcatcacggc tgccgggggc ctcgcggctg cggtccaggc cgacctgtcc cgacccgagg 7020 ggcctgaaga gctgatgcgg gagttcgact ccgcgctcga cggtctcggg ctcgaccgag 7080 ggctcgacat cctcgtcaac aacgccggaa tcagtcggcg cggagcgctc gagcgcgtca 7140 ctgtcgagga tttcgaccgt ctggtcgcac tcaaccagcg cgccccgttc ttcgtgactc 7200 ggcatgccct gccccggatg cacgacggcg gtcgcatcgt caacatttcc tccggatccg 7260 cccgctacgc cagacccgac gtcatcagct acgccatgac caagggggcg atcgaggtgc 7320 tcacccgcgc cctcgccgta gacgtcggcg aacgaggcat caccgccaac gccgtggcgc 7380 cggccgcgct cgataccgac atgaacgcgc actggcttcg cggtgacgac catgcccgca 7440 ccaccgccgc gtccaccact gcactgcgaa aactcgccac cgcggaggac atcgccgcga 7500 tcgtggcctt cctcgtcagc gccgccgccg gtgcgatcac cgggcaggtc atcgacgcca 7560 ccaacggcaa ccggctctaa ccagaactta cccggtccc 7599 // |
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