EBI Dbfetch

ID   AF268074; SV 1; linear; genomic DNA; STD; PRO; 1365 BP.
XX
AC   AF268074;
XX
DT   22-JUN-2000 (Rel. 64, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 2)
XX
DE   Thermomonospora fusca cellulase precursor (Ex) gene, complete cds.
XX
KW   .
XX
OS   Thermobifida fusca
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptosporangineae; Nocardiopsaceae; Thermobifida.
XX
RN   [1]
RP   1-1365
RA   Ai Y.-C., Wilson D.B.;
RT   "Genomic DNA sequence encoding Thermomonospora fusca cellulase with
RT   overexpression";
RL   Unpublished.
XX
RN   [2]
RP   1-1365
RA   Ai Y.-C., Wilson D.B.;
RT   ;
RL   Submitted (15-MAY-2000) to the EMBL/GenBank/DDBJ databases.
RL   Biochemistry, Zhongshan University, 135 Xingang Road, Guangzhou 510275, P.
RL   R. China
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1365
FT                   /organism="Thermobifida fusca"
FT                   /mol_type="genomic DNA"
FT                   /clone="pYCA51"
FT                   /note="overexpressed in Escherichia coli"
FT                   /db_xref="taxon:2021"
FT   sig_peptide     1..93
FT                   /gene="Ex"
FT   CDS             1..1365
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="Ex"
FT                   /product="cellulase precursor"
FT                   /db_xref="GOA:Q9KH72"
FT                   /db_xref="HSSP:1OC7"
FT                   /db_xref="InterPro:IPR001524"
FT                   /db_xref="UniProtKB/TrEMBL:Q9KH72"
FT                   /protein_id="AAF75786.1"
FT                   /translation="MSPRPLRALLGAAAAALVSAAALAFPSQAAANPGEKVDNPFEGAK
FT                   LYVNPVWSAKAAAEPGGSAVANESTAVWLDRIGAIEGNDSPTTGSMGLRDHLEEAVRQS
FT                   GGDPLTIQVVIYNLPGRDCAALASNGELGPDELDRYKSEYIDPIADIMWDFADYENLRI
FT                   VAIIEIDSLPNLVTNVGGNGGTELCAYMKQNGGYVNGVGYALRKLGEIPNVYNYIDAAH
FT                   HGWIGWDSNFGPSVDIFYEAANASGSTVDYVHGFISNTANYSATVEPYLDVNGTVNGQL
FT                   IRQSKWVDWNQYVDELSFVQDLRQALIAKGFRSDIGMLIDTSRNGWGGPNRPTGPSSST
FT                   DLNTYVDESRIDRRIHPGNWCNQAGAGLGERPTVNPAPGVDAYVWVKPPGESDGASEEI
FT                   PNDEGKGFDRMCDPTYQGNARNGNNPSGALPNAPISGHWFSAQFRELLANAYPPL"
FT   mat_peptide     94..1362
FT                   /gene="Ex"
FT                   /product="cellulase"
XX
SQ   Sequence 1365 BP; 242 A; 512 C; 402 G; 209 T; 0 other;
     atgtccccca gacctcttcg cgctcttctg ggcgccgcgg cggcggcctt ggtcagcgcg        60
     gctgctctgg ccttcccgtc gcaagcggcg gccaaccccg gcgagaaggt cgacaacccg       120
     ttcgagggcg ccaagctgta cgtgaacccg gtctggtcgg ccaaggccgc cgctgagccg       180
     ggcggttccg cggtcgccaa cgagtccacc gctgtctggc tggaccgtat cggcgccatc       240
     gagggcaacg acagcccgac caccggctcc atgggtctgc gcgaccacct ggaggaggcc       300
     gtccgccagt ccggtggcga cccgctgacc atccaggtcg tcatctacaa cctgcccggc       360
     cgcgactgcg ccgcgctggc ctccaacggt gagctgggtc ccgatgaact cgaccgctac       420
     aagagcgagt acatcgaccc gatcgccgac atcatgtggg acttcgcaga ctacgagaac       480
     ctgcggatcg tcgccatcat cgagatcgac tccctgccca acctcgtcac caacgtgggc       540
     gggaacggcg gcaccgagct ctgcgcctac atgaagcaga acggcggcta cgtcaacggt       600
     gtcggctacg ccctccgcaa gctgggcgag atcccgaacg tctacaacta catcgacgcc       660
     gcccaccacg gctggatcgg ctgggactcc aacttcggcc cctcggtgga catcttctac       720
     gaggccgcca acgcctccgg ctccaccgtg gactacgtgc acggcttcat ctccaacacg       780
     gccaactact cggccactgt ggagccgtac ctggacgtca acggcaccgt taacggccag       840
     ctcatccgcc agtccaagtg ggttgactgg aaccagtacg tcgacgagct ctccttcgtc       900
     caggacctgc gtcaggccct gatcgccaag ggcttccggt ccgacatcgg tatgctcatc       960
     gacacctccc gcaacggctg gggtggcccg aaccgtccga ccggaccgag ctcctccacc      1020
     gacctcaaca cctacgttga cgagagccgt atcgaccgcc gtatccaccc cggtaactgg      1080
     tgcaaccagg ccggtgcggg cctcggcgag cggcccacgg tcaacccggc tcccggtgtt      1140
     gacgcctacg tctgggtgaa gcccccgggt gagtccgacg gcgccagcga ggagatcccg      1200
     aacgacgagg gcaagggctt cgaccgcatg tgcgacccga cctaccaggg caacgcccgc      1260
     aacggcaaca acccctcggg tgcgctgccc aacgccccca tctccggcca ctggttctct      1320
     gcccagttcc gcgagctgct ggccaacgcc tacccgcctc tgtaa                      1365
//