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EBI DbfetchID AF253421; SV 1; linear; genomic DNA; STD; FUN; 3468 BP. XX AC AF253421; XX DT 06-JUL-2000 (Rel. 64, Created) DT 18-FEB-2004 (Rel. 78, Last updated, Version 3) XX DE Trichoderma atroviride glucan 1,3-beta-glucosidase GLUC78 precursor, gene, DE complete cds. XX KW . XX OS Trichoderma atroviride OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; OC Hypocreomycetidae; Hypocreales; Hypocreaceae; mitosporic Hypocreaceae; OC Trichoderma. XX RN [1] RP 1-3468 RX DOI; 10.1016/S0378-1119(01)00681-3 RX PUBMED; 11602357. RA Donzelli B.G., Lorito M., Scala F., Harman G.E.; RT "Cloning, sequence and structure of a gene encoding an antifungal glucan RT 1,3-beta-glucosidase from Trichoderma atroviride (T. harzianum)"; RL Gene 277(1-2):199-208(2001). XX RN [2] RP 1-3468 RA Donzelli B.G.G., Lorito M., Scala F., Harman G.E.; RT ; RL Submitted (07-APR-2000) to the EMBL/GenBank/DDBJ databases. RL Department of Horticultural Sciences, Cornell University, NYSAES, Geneva, RL NY 14456, USA XX FH Key Location/Qualifiers FH FT source 1..3468 FT /organism="Trichoderma atroviride" FT /strain="P1" FT /mol_type="genomic DNA" FT /db_xref="taxon:63577" FT TATA_signal 163..169 FT /inference="non-experimental evidence, no additional FT details recorded" FT mRNA join(<246..323,512..3468) FT /product="glucan 1,3-beta-glucosidase GLUC78 precursor" FT misc_feature 278..284 FT /note="consensus TATA signal; may indicate alternative FT transcription start site" FT misc_feature 390..398 FT /note="consensus TATA signal; may indicate alternative FT transcription start site" FT misc_feature 442..448 FT /note="consensus TATA signal; may indicate alternative FT transcription start site" FT misc_signal 487..494 FT /note="lariat formation; splicing signal" FT misc_signal 498..505 FT /note="putative U2AF binding site" FT CDS 590..2902 FT /codon_start=1 FT /product="glucan 1,3-beta-glucosidase GLUC78 precursor" FT /function="hydrolyzes glycosidic bonds in beta 1,3 glucans" FT /EC_number="3.2.1.58" FT /note="antifungal protein; synergistic with other enzymes FT that degrade fungal cell walls and other antifungal FT compounds" FT /db_xref="GOA:Q9P491" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/TrEMBL:Q9P491" FT /protein_id="AAF80600.1" FT /translation="MMGLSTVLTASLLALRLFAAPSLAAPAPAADTKAVVEAAAATSWW FT LPNIARNGNVPFSSASGYPVYRNVQTYGAVGDGVHDDTAAINAAISAGNRCGSGCDSST FT TTPAIVYFPPGTYLVSAPIQLWYYTQLIGDATNLPTLKASANFAGIAVIDSNPFRTTPQ FT NNFFRQIRNFKIDLTGQPESTGTGIHWQVAQATSIQNVVFNMNTDTSSNNKQQGIWMED FT GSGGWMSDLTFNGGALGMWVGNQQFTSRNLVFNGCQTAIYMNWNWLWSFQGLTINNANV FT GIDMSQSGVGSILLSDSKISNTKVGVLTGYSTGQNGTAGSLVLDNVDMTSNTPIAVKST FT GGATILNGGATFASWSQGRAYTGTAAGKAVQGTRAAVGKPAALTTGGKVFTKSKPQYET FT VAAGNFVSVKAHGAKGDGSTDDTAAIQAIFNSATSGQVVYFDHGAYVITDTIKVPANIK FT IVGEIWPLIMVGGSKFKDMNNPKPVWQIGQPGDTGNVEIQDLMFETIGPQPGAIIVEWN FT VAGASQGSAGLWDVHWRIGGSAGTQLQSNTCTKTPNQTTSPNSACFGAFLLLHVTSSAS FT IYMENAWLWVADHELDLSDHNQVNIYNGRGLLLESTKGAWLWGTASEHNQLYNYNFNSA FT QNVYSSILQTETAYMQGNPDARVPFTFNSQFADPDFSKCTSATCAGTWGIRVVNSSNTF FT IYGTGMYSFFNNYGQDCVDANNCQDNMIDIQSSSVKLFGISTKASVSMVNLNGQQVVFD FT RDNRNTFCGTAISYETS" FT sig_peptide 590..709 FT polyA_signal 3427..3433 FT polyA_signal 3459..3466 XX SQ Sequence 3468 BP; 759 A; 1036 C; 813 G; 860 T; 0 other; ccatccttct cggtctcatg caaagagcaa tcggcgcgtg atggtggggt ttgagggcgt 60 ttgaaggcca aggggggtgt cgattcaaca ctccatgctt caccacttcc ttcactactg 120 tgctgctctt ttgccagcct tttcccattc actgccgcgg cctattaaag gacctggtcc 180 ggcctcactt ttcacaatcc tctcaccaac acctcttctc atcatcgttg tgttcctccc 240 tgcgcattgg cagctgtctt gcgcatatcc actgtgatag taaagcttcg agcttgaagc 300 ttgtgaggga gaggatattc agagtcagtc cattcttcaa tggttcccgt ctcacaactc 360 ccccctttcc cctcgacaca aagcgcacat atatatttcc cctcttcacg acatcacgct 420 ttcaattgtg caattcatac atataaaagc ctggagaatg gcaattctcc gtttgtttga 480 acactttact aactctgctt ctctcgcata gcctttaccc agtcaacaag atacacatca 540 tcctcgaaac gcgaatcgca gaagagcggc aatcaactgt ttcctagtca tgatgggtct 600 ctcaaccgtc ttgacggcct ctctcctggc cttgcgtcta ttcgcggcgc cttctcttgc 660 tgcaccagcc ccggctgccg ataccaaggc tgtggtcgag gccgctgctg cgacaagctg 720 gtggcttccc aacattgcgc gaaacggcaa tgtgcccttc tcaagtgctt ccggctaccc 780 agtctatcgc aacgtgcaaa cctatggagc cgtgggcgat ggtgtccacg atgacactgc 840 agccatcaac gctgctatta gcgctggaaa ccgatgcggt agcggctgcg actcttccac 900 cactacgcca gccatcgttt acttccctcc cggcacctac ctcgtctcag cgcccattca 960 gctttggtac tacacccagc ttattggtga tgccaccaac ctccctacgc tcaaggcttc 1020 cgccaacttc gccggtattg ccgtcattga ctccaacccc ttccgaacca ctccccagaa 1080 caacttcttc cgccagattc gcaacttcaa gattgacctg actggacagc cagagtccac 1140 tggtaccggt attcactggc aggtcgctca ggctacctcg attcagaacg ttgttttcaa 1200 catgaacacc gacacctcct ccaacaacaa gcagcagggt atctggatgg aggatggctc 1260 tggaggctgg atgagcgacc tcaccttcaa cggtggtgcc ttgggcatgt gggtcggcaa 1320 ccagcagttc accagccgca acctcgtctt caacggctgc cagaccgcca tctacatgaa 1380 ctggaactgg ctgtggagct tccaaggcct gaccatcaac aacgccaacg tcggtattga 1440 catgagccag agcggtgtcg gctccatctt gctctccgac tccaaaatct ccaacacaaa 1500 ggtcggtgtg cttaccggat acagcactgg ccagaacggc accgctggat ccctcgtcct 1560 ggataacgta gatatgactt ccaacacccc catcgctgtc aagagcaccg gcggtgccac 1620 cattctcaat ggcggtgcta cttttgcttc atggtcccag ggtcgcgcct acactggcac 1680 cgccgccgga aaggctgttc agggcactcg tgccgccgtc ggcaagcccg ctgccttgac 1740 taccggtggt aaggtcttca ccaagagcaa gcctcagtac gagacagtcg ctgccggcaa 1800 ctttgtgtcc gtcaaggctc acggcgccaa gggtgacggt tccactgacg acactgctgc 1860 catccaggcc atcttcaaca gcgccacctc tggccaggtc gtctactttg accacggtgc 1920 ctatgtcatc accgatacca tcaaggttcc cgccaacatc aagattgttg gtgagatctg 1980 gcctctcatc atggttggtg gttccaagtt caaggacatg aacaacccca agcccgtctg 2040 gcagattggc cagcccggcg atactggcaa tgttgagatt caagacctca tgttcgagac 2100 catcggcccc cagcccggtg ccatcattgt cgaatggaac gttgctggcg cttctcaggg 2160 ctctgccggc ctgtgggacg tccactggcg tattggcggc tctgctggca ctcagctgca 2220 gtccaacact tgcaccaaga ctcccaacca gacgacttct cccaactcgg cttgctttgg 2280 cgctttcttg cttctgcacg tcacctcttc tgctagcatc tacatggaga acgcttggct 2340 ctgggtcgct gatcacgagc ttgatctctc tgaccacaac caggtcaaca tttacaacgg 2400 ccgtggactt ttgcttgaga gcaccaaggg tgcctggctc tggggcaccg cttccgagca 2460 caaccagctc tacaactaca acttcaacag cgctcagaat gtttacagca gcatcctcca 2520 gactgaaacc gcctacatgc agggcaaccc tgacgcccga gttcctttca ccttcaactc 2580 ccagtttgct gaccctgact tttccaagtg cacttctgcc acctgcgctg gtacctgggg 2640 tatccgtgtg gtaaactcca gcaacacgtt catctacggc actggcatgt acagcttctt 2700 caacaactac ggccaggact gtgtcgacgc caacaactgc caggacaaca tgattgacat 2760 ccagagctca tccgtcaagc tctttggtat cagcaccaag gccagtgtca gcatggtcaa 2820 ccttaacggc cagcaggttg ttttcgacag ggacaaccgc aacactttct gcggcactgc 2880 catttcatat gagacgtcat agaagggcga cgaagatgca gtggctgctg cccccgggcc 2940 taaaaagaat cccggcaatg aaagtgagtc gacgagctct gtggctgaga atccgggcaa 3000 gccaaacttg tctggaactt tcacttttcc cacgtcgact gaatcgctgc cgttgctggg 3060 atgtggtccc gagaagacgg tagtcgtgac tcggaccgta tttgctactg caagcacttc 3120 aaatatttga acatattatg attgatgcgt gtatatatac gtggaatatg ctgtggattt 3180 gtctcatatt tcatttcatt tctttacttt gaattctcat cccgtctttg tcctatataa 3240 actctagtcg ttttggagat ttttagggtg cttgccaata gcagcggatg agaatttcta 3300 tttccttgtg tgatttttag taccaaagtc atcatttttg gttttatttt tgggaacggc 3360 gtggtcagga tttggttttg agccctacgt cagaaaagga agggcacttg aagtgcattc 3420 tgggatagat gtattccaat ggcggactgg atagaatgaa ttgaaaga 3468 // |
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