 |
|
EBI DbfetchID AF247972; SV 2; linear; genomic DNA; STD; PRO; 3629 BP. XX AC AF247972; XX DT 08-JUN-2000 (Rel. 64, Created) DT 25-MAR-2009 (Rel. 100, Last updated, Version 5) XX DE Thalassobius gelatinovorus AgeI restriction-modification system, complete DE sequence. XX KW . XX OS Thalassobius gelatinovorus OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; OC Rhodobacteraceae; Thalassobius. XX RN [1] RP 1-3629 RA Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.; RT "Method for cloning and producing AgeI restriction endonuclease in E.coli"; RL Unpublished. XX RN [2] RP 1-3629 RA Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.; RT ; RL Submitted (22-MAR-2000) to the EMBL/GenBank/DDBJ databases. RL New England Biolabs, Inc., 32 Tozer Rd., Beverly, MA 01915, USA XX RN [3] RC Amino acid sequence updated by submitter RP 1-3629 RA Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.; RT ; RL Submitted (27-AUG-2002) to the EMBL/GenBank/DDBJ databases. RL New England Biolabs, Inc., 32 Tozer Rd., Beverly, MA 01915, USA XX RN [4] RC Sequence update by submitter RP 1-3629 RA Xu S.-Y., Maunus R.E., Lunnen K.D., Allen R.; RT ; RL Submitted (24-MAR-2009) to the EMBL/GenBank/DDBJ databases. RL New England Biolabs, Inc., 32 Tozer Rd., Beverly, MA 01915, USA XX DR RFAM; RF00005; tRNA. XX CC On Mar 24, 2009 this sequence version replaced gi:8037878. XX FH Key Location/Qualifiers FH FT source 1..3629 FT /organism="Thalassobius gelatinovorus" FT /mol_type="genomic DNA" FT /db_xref="taxon:53501" FT CDS 522..1202 FT /codon_start=1 FT /transl_table=11 FT /product="putative transposase" FT /db_xref="UniProtKB/TrEMBL:Q9KHV7" FT /protein_id="AAF71524.1" FT /translation="MGPSTLKRRKPMTKTTYIATAPDGSELTRKTDRTYTHAVLLEGKE FT GWKAEGFCGRLDLAHKKQLEHPGSIVVEVKALGDAQTDKPKAEATEDAEPTKDETVGRP FT EEEPSVDEKIRNAKVTGPERKGKIGDLVHELLMDETLDYVTIVDRVMANFLMPKPRHVQ FT SHLWRLFSARRVLKYPNAGNPRYDLMRATCRKGRCHWSSGDVLNIFGVPLVIHTRYGVC FT KYPR" FT gene 1236..2525 FT /gene="ageIM" FT CDS 1236..2525 FT /codon_start=1 FT /transl_table=11 FT /gene="ageIM" FT /product="AgeI methylase" FT /function="modify ACCGGT sequence (C5M)" FT /note="M.AgeI" FT /db_xref="GOA:P94147" FT /db_xref="HSSP:1DCT" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P94147" FT /protein_id="AAF71525.1" FT /translation="MKTIDLFCGAGGLGEGFRQAGFSALYANDHETPALATYKENHPDA FT VCSTDSIETVDPKKIREDLGVAPGQVDVVMGGPPCQGFSTYGQRRDDDARNQLYVPYFG FT FVEEFRPKAFLIENVVGLLSMSGGAVLADMVARAEALGYAADVVTLDACEYGVPQHRRR FT VFIFGAADGQRIDPPQPSHVNGKRSGVVLNDQPSLFFDGPSIQPALTVRDAISDLPDEV FT LVPRDTQKPMEYPEPPKTEYQRLMRGNSTELTHHSAKRMLGIRRLRLAMLHPGDYGTKI FT EERLADGGLNDELIDLMMGGAGMRDAAECRTQDREKEAALREVLKGGHTTPAKVMEFLD FT SQGFANKYRRLRWDAPSHTVVAHMARDCSDFVHPGIDRFVSVREAARFQSFPDTYRFPG FT SQFRQFRQIGNAVPPLLGRAMAETIKVAIS" FT gene complement(2522..3358) FT /gene="ageIR" FT CDS complement(2522..3358) FT /codon_start=1 FT /transl_table=11 FT /gene="ageIR" FT /product="AgeI endonuclease" FT /function="Cleave A/CCGGT" FT /db_xref="GOA:Q9KHV6" FT /db_xref="UniProtKB/Swiss-Prot:Q9KHV6" FT /protein_id="AAF71526.3" FT /translation="MRLDLDFGRGLVAHVMLDNVSEEQYQQISDYFVPLVNKPKLKSRD FT AIGQAFVMATEVCPDANPSDLWHHVLYRIYIREKIGTDPSQSWVRTSGEAFEVALVERY FT NPVLARHGIRLTALFKGQKGLALTRMGVADRVGSRKVDVMIEKQGGGRSPDAEGFGVVG FT GIHAKVSLAERVSDDIPASRIMMGEGLLSVLSTLDVKSFPPPHGDLVNRGELGTPDRPS FT DKRNYIEGHGDFSACFSYNLRTSPSNATTPSGRHIYVSGFSGQDDEFTDYLVAQLA" XX SQ Sequence 3629 BP; 834 A; 990 C; 1031 G; 774 T; 0 other; tcgaatacat caatggcttc tataatccgc gccgacgcca ttcagcactg ggctggaaaa 60 gccccgtcgc ttttgagcgg aaggtggctt aaacgagcac ttggggcggc acgaaaccgt 120 gacaggtcca ctccagttgg gttcctttgg ttagagtgtg acgcatgaag ggtgctgcga 180 taaccgtcag ttcacaaagg atcgcatggt acgcaccagc atttgcctca gagccaccaa 240 ccttggtttc caatgacgcc gccgccaaaa cccattcggc tgtcaagctc aacgctgccc 300 aaacaaatac ggtgtctgcg gctgttcttg gctctcaata atcgcaattt cctaaaccaa 360 atcaattctg taggggcctc gcaccttatg actgaatatc agttggatgg gcgtattcac 420 acttggctgc caaatgatct ggccagatac ttcccgctga agcaattcgt tgttgcgcaa 480 aatcttttcc ccggctcgca aggtcccagc ttcccgacaa catggggcca tcaacgctga 540 aaaggagaaa accaatgacc aaaaccacct atatcgccac cgcacctgat ggatcggaac 600 tcactcgcaa gaccgaccga acctacaccc acgcggtctt gttagagggc aaggagggct 660 ggaaagctga ggggttctgc ggacgtctcg acctggccca caagaagcaa cttgagcatc 720 ctggcagtat cgtcgtggaa gtcaaagcgc tcggtgacgc acagaccgac aagcctaagg 780 ccgaagctac cgaagacgct gaaccgacca aagatgagac ggttggacgc cccgaagaag 840 agccgagcgt cgatgagaaa atcaggaacg caaaggttac aggtcccgag cgcaagggca 900 aaatcggaga ccttgtgcac gagttgttga tggacgagac gttggattat gtgacgatcg 960 tggatcgggt catggcaaat ttcctgatgc caaaaccacg gcacgttcag tcgcatctgt 1020 ggcggctgtt ctccgcaaga agggtgctga agtacccaaa cgccggaaat ccaaggtatg 1080 acctcatgcg ggcaacttgc cgaaaggggc gttgtcactg gtctagtggc gacgtcctca 1140 atatatttgg cgttccgttg gtcattcata cccgatatgg tgtctgcaaa tatcctcgct 1200 gagaacgtag cgagaactaa ttcggatggg aaaacatgaa gacgatcgat ctattttgcg 1260 gggctggagg attaggagaa ggctttagac aggcaggatt ttcagcgctg tacgccaatg 1320 accatgagac ccctgcgctt gcaacataca aggaaaacca tccagacgca gtatgctcga 1380 ctgattccat cgagactgta gaccccaaga aaattcgcga agaccttggc gtcgcgcctg 1440 gacaggttga cgtggttatg ggggggcctc cctgtcaagg cttctcaacc tacgggcagc 1500 gacgcgacga cgatgcgagg aaccaactgt acgtcccgta tttcggtttc gttgaagagt 1560 tccgacctaa ggcatttctg atcgagaacg tggtcgggtt gctctcaatg tctggaggcg 1620 cggtacttgc agacatggtc gcccgcgcag aggcactcgg ttatgctgct gacgtggtaa 1680 ccttggacgc gtgcgagtat ggggtgccgc agcatcgtcg ccgtgtcttc atctttggtg 1740 ccgcagacgg ccagcgtatt gatcctcccc aaccgtctca cgttaacggt aagcgtagcg 1800 gtgtcgtgct aaacgatcag ccttcgctgt tcttcgatgg tccgtcgatc cagccagctc 1860 tgactgttcg cgatgctatt tcggacctgc ctgatgaggt gctggtgccg cgtgacactc 1920 aaaaaccgat ggaatatccc gagccgccta agaccgagta tcagcggttg atgcgaggta 1980 attccacgga gctaacccat cactcggcaa aaagaatgtt aggtatccgc cgtttacggt 2040 tggcgatgct tcatcctggt gactacggga ccaagatcga agaacggctg gctgacggcg 2100 gcctaaatga cgagctcata gacttgatga tgggtggagc tggaatgcgc gatgccgcag 2160 agtgccgtac tcaggaccga gaaaaagagg ctgcccttcg ggaggtgttg aagggaggcc 2220 ataccacacc tgcgaaggtg atggaattcc tggatagtca agggttcgca aacaagtacc 2280 gtcggttacg ctgggatgca ccatcgcaca cggtcgtcgc gcacatggct cgggattgtt 2340 cagacttcgt gcaccccggc attgatcgct tcgtctcggt gcgagaggct gcaaggttcc 2400 agtcttttcc tgacacctat cgatttccag gctcgcagtt ccgccagttc cgccaaattg 2460 gaaacgcagt cccaccgttg ctaggcaggg caatggctga aacaataaag gttgcgatca 2520 gttaggccag ttgggcgact aagtagtcgg tgaactcgtc gtcctgacca gagaagccgc 2580 tcacgtatat gtggcgtccg ctgggcgttg ttgcgttgga cggcgaggtc cgcaggttgt 2640 agctgaaaca ggccgagaaa tccccgtgtc cctcaatgta attcctcttg tccgagggcc 2700 ggtcgggcgt gccaagctcg cctcggttca ccaaatcgcc gtggggcgga gggaacgact 2760 tgacgtcgag ggtggagagc acgctgagga gaccctcgcc catcatgatc ctgctggcgg 2820 gtatgtcgtc cgagaccctc tcggctaggc tcaccttggc gtggatgcca cccacgacgc 2880 cgaatccctc ggcgtccgga gagcgtccgc ctccctgctt ctcgatcatc acgtcaacct 2940 tgcgagagcc gacgcggtcg gccacaccca tacgcgtcag tgcaaggccc ttctgcccct 3000 tgaataaggc ggtcaacctg atcccatgtc gggccagcac tggattataa cgctcgacca 3060 gcgcgacctc aaaggcctcg cccgacgtgc gaacccagct ctggcttggg tcggttccga 3120 tcttctcgcg tatgtagatg cggtacaaga cgtggtgcca gaggtctgag gggttggcgt 3180 ccggacagac ttccgtcgcc attacgaaag cctgaccgat agcgtcgcgg ctcttaagct 3240 tcggcttgtt caccagcggc acgaagtagt cggagatttg ctggtactgc tcctcgctta 3300 cgttatccag catcacatgg gcgaccagtc cacgcccgaa gtccaagtcc aatctcaagt 3360 caaatccccc gccttcataa ttacaccaca tagtggggca aatcggtgag gaacttcaag 3420 aggttgtatg cggtgttgcg ccgattgcct ttggcagccg ggaacagtgc atggcgagaa 3480 tactgtactt attctgtact cgaaaagaaa aaggacttag cggatgagcg ctaagtcctt 3540 gaaatttttg gctccggcgg tagggatcga acctacgacc aattgattaa cagtcaactg 3600 ctctaccgct gagctacgcc ggaacatgt 3629 // |
|