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EBI DbfetchID AF247191; SV 1; linear; genomic DNA; STD; PRO; 3529 BP. XX AC AF247191; XX DT 11-NOV-2000 (Rel. 65, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Desulfurococcus mucosus pullulanase (ApuA) gene, complete cds. XX KW . XX OS Desulfurococcus mucosus OC Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales; OC Desulfurococcaceae; Desulfurococcus. XX RN [1] RP 1-3529 RX DOI; 10.1128/JB.182.22.6331-6338.2000 RX PUBMED; 11053376. RA Duffner F., Bertoldo C., Andersen J.T., Wagner K., Antranikian G.; RT "A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, RT sequencing, purification, and characterization of the recombinant enzyme RT after expression in Bacillus subtilis"; RL J. Bacteriol. 182(22):6331-6338(2000). XX RN [2] RP 1-3529 RA Duffner F.M., Bertoldo C., Andersen J.T., Wagner K., Antranikian G.; RT ; RL Submitted (20-MAR-2000) to the EMBL/GenBank/DDBJ databases. RL Enzyme Research, Novo Nordisk A/S, Novo Alle, Bagsvaerd 2880, Denmark XX FH Key Location/Qualifiers FH FT source 1..3529 FT /organism="Desulfurococcus mucosus" FT /mol_type="genomic DNA" FT /db_xref="taxon:2275" FT CDS 1196..3256 FT /codon_start=1 FT /transl_table=11 FT /gene="ApuA" FT /product="pullulanase" FT /function="starch degrading enzyme" FT /db_xref="GOA:Q9HHB0" FT /db_xref="HSSP:1J0H" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9HHB0" FT /protein_id="AAG31033.1" FT /translation="MGWRPVAVYATLILVLLQLTPLPIAGASIMEIYVADDQVTVVHNP FT LDPAYLSAADGYLIPRIRVASSLDVASGTLVADKGEYQLKPQLATNTWRVYYATIPIGE FT ASRGLNYYFKLTLRNNTVVYVYNATASRLFNFNGSIVFRQVEWVKSRVGYQIFPDRFYN FT GDPSNDLKANLTDELWINEVSRGVPVFTRWDGPVTSLHCCHQYFGGDLKGVTEKLDYLK FT ELGVGLIYLNPIFLSGSVHGYDTYDYYTVDPKFGTLEDLKTLINEAHKRGIKVIFDFVP FT DHVGLGFWAFQDVYRNGRNSTYWSWFIVYKWRFKLGDPTAYKCWWGIGSLPQLNVLNTE FT VRQYLINVALYWLSIGFDGLRIDTPLDVIDSESFFRELREAVKSRYPDAYIVGEIWDYR FT PEWLRGNAFDSLMNYYLGRNILLSYARGALNGYTASMKLAEYYAGIGVNVAGMGFNIIG FT SHDTSRVLTDLGGGGLNSTPSNESIARLKLLSTLQYTQPGMPVVFQGDERGITGRQGNH FT DEQRYPIQWDRLNVEVYEHYKRLGELKNTIPALSTSIIHVLGGSGGLLAYTRGYMDEVL FT VIANNDASTPQSYELPPGNWTLIYASNNWSEVSVEHNTVTVPPLTALILVRNTVSETTT FT TSTAVTSFPGTMYTETTAIPGRLEQDTRVLIIVVAVPLLLATLVLLRRHRA" XX SQ Sequence 3529 BP; 898 A; 877 C; 969 G; 785 T; 0 other; gatcacgcct gcttcatcat atacggcgga tacctggggg ttcacgggca ccggtaaccc 60 ctctacgtac acaaatatag ggtgcgtgta ctccacggat tctccagcgg ccgaagggtt 120 gtagaggaag gtttgaatcc tgttcaacag tattctcaca taatccatgc tactaaatga 180 gacctccacg ctgccacggt agaccacgag tgacactgtg tcgctggcat tgtttgcagt 240 gaccgagagg aggtatgtgg atgggggaga tgctacagta tagtagacgc tgtaggcgaa 300 gccggctgca acgagcacga ggaggactgt gagaaccgcc ttggcgactc ctcctagtcc 360 acggggcttc ctgacaacta tgcctatttc ctgtagccat ctcgcaatgt aatcgtatgt 420 atagttcaac ctgtagtgct cgacgaggcc ccttgtaagc acatatgtca aggcgaggcc 480 tgctacgaag ccacctacat gtgcgaagta tgctacaccg cccgagcctc atgtagcccg 540 tagagtactt gctcagcgaa ccatattatg aggaagacag ctgttgaaac cgggaagcag 600 tatggcagga acaggaagaa catgcaccac atgagccttg cataggggaa gagcagcagg 660 taggctccca gcaccccgct tatagcaccc gacgccccaa ccgccggtac tactagcgag 720 tcgtatccgc cgaccgggat tatggctaca tggaataaca ctgctgcaag ccctgaggca 780 aagtagagga gtaggtatct ccgggagccg atgacgctct cgattcttct accgaagagc 840 cataggaagt acatgttgaa gaatatgtgg aagaagtctg catgcgtgaa cattgctgtg 900 aatattctta caacaccctg caaggggtcg gtgaagagta atgcgggtat gaagcctagg 960 gcgtagatgc tttcattcgt agactcaagg aacatgtttc gataacttgt gtaaaggtat 1020 acgacgatgt tcgccgccac tattagcgtt gtcgccttag aagagcccat accccttgaa 1080 cgctcccatg gcacgacaac cattagcacg ccctcgcgaa actcctggtt gataattagc 1140 cgggtgttta ataaagcttg catacatagg gcaagggtgt ggaggtgctt aaccagtggg 1200 ctggaggccg gttgctgtat acgctactct aatactcgta ttactccagt tgacgcccct 1260 acctatcgct ggtgcgagca tcatggagat atatgtcgcc gatgaccagg tcaccgtggt 1320 acacaacccc ctggatccag catacctttc agcagccgac ggctatttga tcccgaggat 1380 aagggtggcc agcagcctgg atgttgcctc tgggacgctg gtagctgata aaggagagta 1440 ccagttgaaa ccccagttgg cgacgaacac gtggagagta tactatgcca caatacccat 1500 tggtgaggca tccaggggtt taaactacta tttcaagctc accctgcgga acaacactgt 1560 ggtgtacgtg tataatgcga cggcgagcag gctattcaac ttcaatgggt caatagtttt 1620 caggcaggtg gagtgggtta agagccgggt tggctaccag atattccccg atagattcta 1680 caatggtgat ccaagcaacg atttaaaggc caacctaacg gatgagctat ggataaacga 1740 ggtttcaagg ggcgtacccg tattcactag gtgggatggc cctgtaacat cgctacattg 1800 ctgccaccag tatttcggcg gcgacctgaa gggggtcaca gagaagctcg actacctcaa 1860 ggagctcggt gttgggctaa tatatctgaa ccctatattc ctctccggca gcgtacacgg 1920 ctacgacact tacgactact atactgtgga cccgaagttc gggaccctgg aagaccttaa 1980 aaccctcatc aacgaggcgc ataaacgggg cattaaagtg atattcgact tcgtcccaga 2040 ccacgtgggg cttggattct gggctttcca agacgtttac aggaacggaa ggaacagcac 2100 gtactggagc tggttcatag tgtataagtg gaggttcaag ctcggggacc ccaccgcgta 2160 taagtgctgg tgggggatag ggagcctccc gcagctgaat gttctgaaca ctgaggttag 2220 acagtacctg atcaatgtag ccctatactg gttaagcatc ggcttcgatg ggttgaggat 2280 tgatactccg ctagacgtca tcgactcgga gagcttcttc agggagctac gtgaagcagt 2340 caagtcgagg tatcccgacg catacattgt tggagagata tgggattacc gtcccgaatg 2400 gctaaggggc aatgcattcg actcccttat gaactactat ttaggcagga acatactcct 2460 cagctatgca cgtggagccc tgaacggtta caccgcctca atgaagcttg ctgaatacta 2520 tgccggtata ggtgtgaacg tggctggaat gggtttcaac attattggat cccatgacac 2580 ctccagggtt ctcacggatc tcggcggggg aggattgaac agcaccccga gcaatgagtc 2640 catagcccgc ttaaaactgc tttcaacgct acagtatact cagcccggta tgccagtagt 2700 gttccagggc gatgaaagag ggatcactgg tagacaggga aaccatgatg agcagagata 2760 ccctattcaa tgggataggt taaatgtaga ggtctacgag cactataaga ggctgggaga 2820 actcaagaac actattccag cattgtcaac cagtataata catgtgctgg gtggatcagg 2880 cggcttgctt gcctatacta gggggtatat ggatgaagta ctcgtcatcg ccaataatga 2940 tgcatccaca ccgcaatcat acgagctgcc cccgggcaac tggaccctga tatatgctag 3000 caataactgg agcgaggtct ccgtcgagca caatacggtt acagtgccgc ctttgacagc 3060 cctgatactt gtcaggaaca ctgtgtccga gaccactact acatcgacag ctgtgaccag 3120 cttccccggc accatgtaca cggaaaccac cgctattcca ggccgactgg agcaggacac 3180 cagagtgctg attatcgtag tagccgtgcc gctgctcctt gcgacactag tattgctccg 3240 caggcatagg gcttaacgcg tagaggagaa ggcttggagt agcgtgaaag gattaaacgg 3300 tttaagccgc ctagatataa acggcgatga agagccgtcg aacggccgaa gaggtgatga 3360 gtcgctctcc tccgagccct ccagattcgc aacgaaccta gaggtttctc aaggcatgag 3420 gcaggctacg gggcaccggt tcctcgtgca ccccgggccc gctgcactat ggattccact 3480 gggggggggg agccctgtct tttaaggctg gatgctagtg tacttgatc 3529 // |
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