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EBI DbfetchID AF242413; SV 1; linear; genomic DNA; STD; PRO; 3100 BP. XX AC AF242413; XX DT 25-OCT-2000 (Rel. 65, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Paenibacillus alginolyticus XalA (xalA) gene, complete cds. XX KW . XX OS Paenibacillus alginolyticus OC Bacteria; Firmicutes; Bacillales; Paenibacillaceae; Paenibacillus. XX RN [1] RP 1-3100 RX PUBMED; 10347025. RA Ruijssenaars H.J., de Bont J.A., Hartmans S.; RT "A pyruvated mannose-specific xanthan lyase involved in xanthan degradation RT by Paenibacillus alginolyticus XL-1"; RL Appl. Environ. Microbiol. 65(6):2446-2452(1999). XX RN [2] RP 1-3100 RX DOI; 10.1128/AEM.66.9.3945-3950.2000 RX PUBMED; 10966413. RA Ruijssenaars H.J., Hartmans S., Verdoes J.C.; RT "A novel gene encoding xanthan lyase of Paenibacillus alginolyticus strain RT XL-1"; RL Appl. Environ. Microbiol. 66(9):3945-3950(2000). XX RN [3] RP 1-3100 RA Ruijssenaars H.J., Hartmans S., Verdoes J.C.; RT ; RL Submitted (08-MAR-2000) to the EMBL/GenBank/DDBJ databases. RL Industrial Microbiology, Wageningen University, P.O. Box 8129, Wageningen RL 6700 EV, The Netherlands XX FH Key Location/Qualifiers FH FT source 1..3100 FT /organism="Paenibacillus alginolyticus" FT /strain="XL-1" FT /mol_type="genomic DNA" FT /db_xref="taxon:59839" FT -35_signal 22..36 FT /gene="xalA" FT /inference="non-experimental evidence, no additional FT details recorded" FT -10_signal 58..65 FT /gene="xalA" FT /inference="non-experimental evidence, no additional FT details recorded" FT RBS 72..81 FT /gene="xalA" FT /inference="non-experimental evidence, no additional FT details recorded" FT sig_peptide 86..193 FT /gene="xalA" FT CDS 86..2896 FT /codon_start=1 FT /transl_table=11 FT /gene="xalA" FT /product="XalA" FT /function="removes pyruvated mannosyl residues from xanthan FT side chain via beta-elimination mechanism" FT /note="pyruvated mannose-specific xanthan lyase; FT extracellular enzyme" FT /db_xref="GOA:Q9F8Q9" FT /db_xref="HSSP:1J0M" FT /db_xref="InterPro:IPR012329" FT /db_xref="UniProtKB/TrEMBL:Q9F8Q9" FT /protein_id="AAG24953.1" FT /translation="MSLSRSLARRNYVQVIVASLLSLVLLLSWLPTPANASDEYDTLRL FT KWRDMLIGGSSVSTSDPDVVTAITTLTAEAQTNWTNLNKTPGGTTNYLWVDLRSSSDSS FT QLTTAYNRIKSMALASQTPGSTLYGNTDLIADTIMALDWMDTWKYYATKTSYQNWWDWQ FT IGVPLALNDTVVLMYDLLSSTQIANYMAAVDRFAPTVDKTGANRVWKATAVGIRAVIVK FT DSVKLAAARDGLTSVFPNVTSGDGFYNDGSFIQHNYFGYTGGYGASILMDLANLMYLLS FT GSTWAVTAPEQSNLYQWIYNAYQPLLYKGAMMDMVRGREISRYYAQDRNTGHLIMQGII FT RVSQFAPATDAAAYKAMIKSWISSENSIGTGVKFYGDASVNMITLAKAIMANASITAAP FT ELVKYQQFAAMDRAVFLRPGFGLGIGMFSTRTQNYESINSENAKGWYSGAGTTYLYNND FT LDQYSDNYWPTVDNYRLPGTTVISQTSTTAQFGTNNWVGGTDLLGIYGTTGMAQGVASN FT ALTAKKSWFTFDDEIVALGAGISSTDNATIESIVENRKLNSAGTNALTVNGTAQSTALG FT WTQSLTNVNTIHMAGNVAGSDIGYYFPTAPTIQAKREARTGNWKQINSRASTPSTPYTR FT NYLTMWFDHGVNPTNASYQYVILPNKTSAQVSSYASNPNISVLENSNAAQAVKENTLNV FT VGVNFWNDAVKTVGGITSNKKASVMTKETASDLSVSVSDPTQANTGSIQIEINKAATGI FT ISTDTGVTVTQLSPTIKFTVNVGASKGKSFKAKFDFTGTVTPPTPPAGTTVIVDNAGSG FT VTKVGSWTTASTATDKYLTNYVHDSNTGQGTKSVTLTPTLTGAGNYNVYMWWPAHANRA FT TNIPVDIGYDGGSTTVTINQQLNGGQWNLIGTFPFAAGSGGYVKVRNDGANGYVTVDAV FT KFDPVP" FT repeat_region 2959..2987 XX SQ Sequence 3100 BP; 777 A; 786 C; 859 G; 678 T; 0 other; tagggcatta tcctaatgtt cttttttatt tataatgaag ttgagtaaag cgctttcaaa 60 atatacatgc gaaagaggga atatgatgag tttatccaga agcttagcgc gcagaaatta 120 tgtacaggtc atcgtcgcat ccttgctctc cctggtgctg ctgcttagct ggctgccgac 180 tccggcaaat gcctcggatg agtatgatac gctgcgccta aagtggcgcg atatgttgat 240 cggcggttca tccgtatcga cgtccgatcc ggatgttgtg acggcgatta cgacgcttac 300 cgcggaagcg cagacgaatt ggaccaacct gaacaaaacg ccgggaggta cgacgaacta 360 tctgtgggtc gatcttcggt cgtcctccga ttcgtcccaa ctgaccaccg cgtacaatcg 420 gattaaaagc atggcgctcg cctcccaaac gccgggctct acgctgtacg gcaacacaga 480 tttgattgcc gatacgatca tggcgctgga ttggatggac acctggaaat actatgcgac 540 caaaacgtct taccaaaact ggtgggattg gcaaatcggc gtgccgctgg cactcaacga 600 cacggttgtg ttgatgtacg atctgctctc ttcaacgcaa attgccaact atatggcggc 660 tgtcgataga ttcgcgccga ccgtagacaa aacaggtgcg aatcgcgtct ggaaagcgac 720 ggctgtcggc attcgcgcgg tcattgtgaa agacagcgtg aagcttgctg cggcccggga 780 cggtcttaca tccgtattcc cgaatgtgac gtcgggcgac ggcttttaca atgacggttc 840 cttcattcag cacaattact tcggatacac cggggggtat ggggcaagca tcctcatgga 900 tttggctaac ttgatgtact tgctcagcgg ttcgacatgg gcggtgacgg cgccggagca 960 atcgaatctt tatcagtgga tttacaatgc gtatcagcct ttgctgtaca aaggggcgat 1020 gatggatatg gtgcggggtc gtgaaatttc ccgatactat gcacaggatc gcaacacagg 1080 ccatctgatt atgcagggca tcattcgcgt atcgcagttt gctcccgcca cggatgcggc 1140 ggcttataaa gcgatgatta aaagctggat ttcatccgaa aattcgattg gcacaggggt 1200 taaattctac ggggacgcca gcgtcaacat gattacatta gctaaagcga ttatggcgaa 1260 tgcttcaatt acggcagcgc ctgaacttgt caaataccaa cagttcgctg cgatggaccg 1320 cgccgtgttc ctgcgtccgg gctttggtct gggcatcggc atgttctcaa cgcgaaccca 1380 aaattacgag tcgattaata gcgagaacgc caaaggatgg tacagcggtg cgggcacgac 1440 gtatttgtac aataatgatc ttgatcaata cagcgacaac tactggccaa cggttgacaa 1500 ttaccggttg ccgggcacga cggtcatctc gcaaacgagt actacggcgc aattcggtac 1560 gaacaactgg gttggcggca ctgatctgct tggcatctat gggacgaccg gcatggcgca 1620 gggagttgct tccaatgcac tgacggcaaa gaaatcctgg tttacgttcg atgacgaaat 1680 cgtggctctt ggtgcgggca tttccagcac ggacaacgca acgatcgaat cgattgtcga 1740 gaaccgcaag ctgaacagcg caggcacgaa tgcgcttacg gtgaacggca cagcgcaatc 1800 gacggcactg ggctggaccc agtcgctaac gaatgtaaac acgattcaca tggctggcaa 1860 tgtggccggc tccgacatcg gctactactt cccgacagcc ccgacgattc aagcgaagcg 1920 ggaagcgaga accggcaact ggaagcaaat taattccagg gcgtcaacgc cttccacacc 1980 ttacacgcgc aattatttga cgatgtggtt cgatcacggg gtgaacccga cgaatgcttc 2040 gtatcaatat gtgattttgc cgaataaaac aagtgctcag gtgagcagct acgccagtaa 2100 cccgaatatc tccgtgctgg agaattccaa cgctgcgcag gcggttaaag aaaatacgtt 2160 gaacgtcgtt ggcgttaact tctggaacga tgcggtgaaa acggtcggcg gcattacgtc 2220 caacaagaag gcgtccgtca tgacgaagga gacggcaagc gatctgagcg tctccgtctc 2280 ggatccgacc caggcgaata ccggcagcat tcagatcgag atcaataaag ctgcaaccgg 2340 cattatttct actgataccg gcgttacggt aacacagctc agcccaacga ttaaatttac 2400 ggtaaatgtc ggggcgtcta aaggcaaatc gtttaaggcg aaattcgatt tcaccggtac 2460 ggttacaccg ccgacgccgc cagccggcac aacggtgatc gtcgataacg ctggctccgg 2520 cgtgacgaaa gtcggctcat ggacgacggc ctcaacggca accgataaat atttgacgaa 2580 ctatgtgcat gacagcaaca cgggtcaagg cacgaagagc gtgacgctga cgccgacgtt 2640 gacaggagca ggcaactaca acgtgtacat gtggtggcct gcgcacgcca accgtgcgac 2700 gaacattcca gtcgatattg gatatgacgg aggcagcacg accgtgacca tcaatcagca 2760 gctgaacggc ggccagtgga acttgatcgg cacgttcccg tttgctgcag gctcgggcgg 2820 gtatgtgaaa gttcgtaacg atggcgcgaa cgggtacgta accgtggatg cggtgaaatt 2880 cgatccggtg ccttaacatc cgggcgcggc tgcatggctc cgcccatcac ttccgtattt 2940 gaagacgagc gaacggagta agaagaagcc ccttcgggct tcttcttgca gctttgggga 3000 gggaagatcc cccttcgcca acaagcatct ttatcttcca tgtttattac gccgcatgga 3060 gaaggccaaa ggctttccag aaaaatggat cggcttgtgc 3100 // |
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