EBI Dbfetch

ID   AF233376; SV 1; linear; genomic DNA; STD; PRO; 1116 BP.
XX
AC   AF233376;
XX
DT   02-AUG-2000 (Rel. 64, Created)
DT   02-AUG-2000 (Rel. 64, Last updated, Version 1)
XX
DE   Streptomyces sp. 11AG8 cellulase 12A (cel12A) gene, complete cds.
XX
KW   .
XX
OS   Streptomyces sp. 11AG8
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
XX
RN   [1]
RP   1-1116
RA   van Solingen P., Meijer D., van der Kleij W.A.H., Barnett C.C., Bolle R.,
RA   Power S.D., Jones B.E.;
RT   "Cloning and expression of an endocellulase gene from a novel Streptomycete
RT   isolated from an East African soda lake";
RL   Unpublished.
XX
RN   [2]
RP   1-1116
RA   van Solingen P., Meijer D., van der Kleij W.A.H., Barnett C.C., Bolle R.,
RA   Power S.D., Jones B.E.;
RT   ;
RL   Submitted (10-FEB-2000) to the EMBL/GenBank/DDBJ databases.
RL   Microbial & Molecular Screening, Genencor International B.V., Archimedesweg
RL   30, 2333 CN Leiden, The Netherlands
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1116
FT                   /organism="Streptomyces sp. 11AG8"
FT                   /strain="11AG8"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:133452"
FT   misc_feature    1..93
FT                   /gene="cel12A"
FT                   /note="Region: signal sequence"
FT   CDS             1..1116
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="cel12A"
FT                   /product="cellulase 12A"
FT                   /note="endocellulase"
FT                   /db_xref="GOA:Q9KIH1"
FT                   /db_xref="InterPro:IPR001919"
FT                   /db_xref="InterPro:IPR002594"
FT                   /db_xref="InterPro:IPR008965"
FT                   /db_xref="InterPro:IPR008985"
FT                   /db_xref="InterPro:IPR013319"
FT                   /db_xref="PDB:1OA4"
FT                   /db_xref="UniProtKB/TrEMBL:Q9KIH1"
FT                   /protein_id="AAF91283.1"
FT                   /translation="MRSHPRSATMTVLVVLASLGALLTAAAPAQANQQICDRYGTTTIQ
FT                   DRYVVQNNRWGTSATQCINVTGNGFEITQADGSVPTNGAPKSYPSVYDGCHYGNCAPRT
FT                   TLPMRISSIGSAPSSVSYRYTGNGVYNAAYDIWLDPTPRTNGVNRTEIMIWFNRVGPVQ
FT                   PIGSPVGTAHVGGRSWEVWTGSNGSNDVISFLAPSAISSWSFDVKDFVDQAVSHGLATP
FT                   DWYLTSIQAGFEPWEGGTGLAVNSFSSAVNAGGGNGGTPGTPAACQVSYSTHTWPGGFT
FT                   VDTTITNTGSTPVDGWELDFTLPAGHTVTSVWNALISPASGAVTARSTGSNGRIAANGG
FT                   TQSFGFQGTSSGAGFTAPAGARLNGTSCTVR"
FT   misc_feature    94..756
FT                   /gene="cel12A"
FT                   /note="Region: catalytic domain"
FT   misc_feature    757..792
FT                   /gene="cel12A"
FT                   /note="Region: linker region"
FT   misc_feature    793..1113
FT                   /gene="cel12A"
FT                   /note="Region: cellulose binding domain"
XX
SQ   Sequence 1116 BP; 191 A; 432 C; 340 G; 153 T; 0 other;
     atgagatccc atccccgctc cgcgacgatg accgtcctcg tcgtcctggc ctcgctcggc        60
     gcgctgctca ccgcagcggc tcccgcccag gcgaaccagc agatctgcga ccgctacggc       120
     accaccacga tccaggaccg gtacgtggtg cagaacaacc gctggggcac cagcgccacc       180
     cagtgcatca atgtgaccgg caacggtttc gagatcaccc aggccgacgg ttcggtgccg       240
     accaacggcg ccccgaagtc ctatccctcg gtctacgacg gctgccacta cggcaactgc       300
     gcgccccgca cgacgctgcc catgcggatc agctcgatcg gcagcgcgcc cagcagtgtc       360
     tcctaccgct acaccggcaa cggcgtctac aacgccgcgt acgacatctg gctggacccg       420
     acaccccgca ccaacggggt gaaccggacc gagatcatga tctggttcaa ccgggtcggc       480
     ccggtccagc ccatcggttc gccggtcggc acggcccacg tcggcggccg cagctgggag       540
     gtgtggaccg gcagcaacgg ttcgaacgac gtgatctcct tcctggcgcc ctccgcgatc       600
     agcagctgga gcttcgacgt caaggacttc gtcgaccagg ccgtcagcca cggcctggcc       660
     accccggact ggtacctcac cagcatccag gcgggcttcg agccgtggga gggcggcacc       720
     ggtctggccg tgaactcgtt ctcctccgcg gtgaacgccg ggggcgggaa cggcggcact       780
     ccggggacac cggcggcctg ccaggtctcc tacagcaccc acacctggcc cggcggcttc       840
     accgtcgaca ccaccatcac caataccggc tccacacccg tcgacggctg ggaactggac       900
     ttcaccctcc ccgccggtca cacggtcacc agcgtgtgga acgcgctgat cagccccgcc       960
     tcgggcgcgg tcacggcacg cagcaccggc tccaacggcc ggatcgcggc caacggcggg      1020
     acccagtcct tcggtttcca gggcacctcc agcggagcgg ggttcaccgc accggccggg      1080
     gcccggctca acggcacctc ctgcacagtg agatga                                1116
//