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EBI DbfetchID AF233372; SV 1; linear; genomic DNA; STD; PRO; 2236 BP. XX AC AF233372; XX DT 09-MAR-2001 (Rel. 67, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus stearothermophilus IMA6503 neopullulanase (npl) gene, complete DE cds. XX KW . XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-2236 RX DOI; 10.1042/BA20010052 RX PUBMED; 11834127. RA Cheong K.-A., Kim T.-J., Yoon J.-W., Park C.-S., Lee T.-S., Kim Y.-B., RA Park K.-H., Kim J.-W.; RT "Catalytic activities of intracellular dimeric neopullulanase on RT cyclodextrin, acarbose and maltose"; RL Biotechnol. Appl. Biochem. 35(Pt 1):27-34(2002). XX RN [2] RP 1-2236 RA Kim T.-J., Chung K.-a., Kim C.-W., Park K.-H.; RT "Cloning of neopullulanase from Bacillus stearothermophilus IMA6503"; RL Unpublished. XX RN [3] RP 1-2236 RA Park K.-H., Kim T.-J., Chung K.-a., Kim J.-W.; RT ; RL Submitted (10-FEB-2000) to the EMBL/GenBank/DDBJ databases. RL Food Science and Technology, Seoul National University, 103 Seodun-Dong RL Kwonsun-Gu, Suwon 441-744, Republic of Korea XX FH Key Location/Qualifiers FH FT source 1..2236 FT /organism="Geobacillus stearothermophilus" FT /strain="IMA6503" FT /mol_type="genomic DNA" FT /db_xref="taxon:1422" FT CDS 217..1983 FT /codon_start=1 FT /transl_table=11 FT /gene="npl" FT /product="neopullulanase" FT /note="Npl6503; maltogenic amylase" FT /db_xref="GOA:Q9AIV2" FT /db_xref="HSSP:1J0H" FT /db_xref="InterPro:IPR013783" FT /db_xref="UniProtKB/TrEMBL:Q9AIV2" FT /protein_id="AAK15003.1" FT /translation="MRKEAIYHRPADNFAYAYDSETLHLRLRTKKDDIDRVELLHGDPY FT DWQNGAWQFQTMPMRKTGSDELFDYWFAEVKPPYRRLRYGFVLYSGEEKLVYTEKGFYF FT EAPIDDTAYYFCFPFLHRVDLFEAPDWVKDTVWYQIFPERFANGNPSISPEGSRPWGNE FT DPTPTSFFGGDLQGIIDHLDYLVDLGITGIYLTPIFRSPSNHKYDTADYFEVDPHFGDK FT ETLKTLIDRCHEKGIRVMLDAVFNHCGYEFAPFQDVWKNGESSKYKDWFHIHEFPLQTE FT SRPNYDTFAFVPQMPKLNTANPEVKRYLLDVATYWIREFDIDGWRLDVANEIDHEFWRE FT FRQAVKALKPDVYILGEIWHDAMPWLRGDQFDAVMNYPFTDGVLRFFAKEEIGARQFAN FT QMVHVLHSYPNNVNEAAFNLLGSHDTSRILTVCGGDIRKVKLLFLFQLTFTGSPCIYYG FT DEIGMTGGNDPECRKCMVWDPMQQNKELHQHVKQLIALRKQYRSLRRGEISFLHADDEM FT NYLIYKKTDGDETVLVIINRSDQKADIPIPLDARGTWLVNLLTGERFAAEAETLCTSLP FT PYGFVLYAIERW" XX SQ Sequence 2236 BP; 590 A; 523 C; 527 G; 596 T; 0 other; ctgcagtcca atcaatagga aatcgtttgc acagataaac taaaactatt tctgcgaaaa 60 cgattgcact gctcatttct attatatcgc ctatttttca ttttacaagc tttttttcta 120 ctgaatttga ctttttttct ctcttttcgt actctttaat cagaggatgg caatcatgca 180 aacgattgct ttcatatatg aaggaggaga ggccaaatga ggaaagaagc catctaccac 240 cgcccggctg acaacttcgc ctatgcctat gatagtgaga cacttcatct tcggcttcga 300 acaaaaaaag acgatatcga tcgtgttgag ctgctgcatg gtgacccgta tgactggcaa 360 aacggagctt ggcagtttca aacgatgccg atgcgaaaaa caggaagtga cgagttgttt 420 gattattggt tcgccgaagt caaacctccc tatcgccggt tacgctacgg gttcgtgctg 480 tattcaggag aggagaagct cgtttataca gaaaaagggt tttactttga ggctccaatc 540 gatgataccg cttactactt ttgctttccc tttcttcatc gagtcgactt attcgaggcg 600 ccggattggg taaaggatac agtctggtat caaattttcc ctgagcggtt cgccaacggc 660 aacccatcaa tcagtccaga aggatcgcgg ccgtggggga acgaggatcc aacaccaacc 720 agcttttttg gcggcgactt gcaagggatt atcgatcatc tcgattacct tgttgacctt 780 ggtattaccg gtatttactt aacgccgatc ttccgttctc cgtcaaacca taaatacgat 840 accgctgatt attttgaagt cgatccacac tttggggata aagaaacgtt gaaaacgctc 900 atcgaccgtt gccatgaaaa aggtatccgc gttatgctcg atgccgtgtt taaccattgc 960 ggctatgagt tcgccccgtt ccaagatgta tggaaaaacg gtgagtcctc aaaatataag 1020 gactggtttc acattcatga atttccgctg caaacagagt cgcggccgaa ttacgataca 1080 tttgcattcg tgccacaaat gccaaagctg aacaccgcca atccagaagt gaagcgttat 1140 ttgcttgatg ttgcgacata ttggattcgt gagtttgaca ttgacggttg gcggcttgat 1200 gttgccaatg aaatcgacca cgaattttgg cgcgagttcc gccaggcagt aaaggcactg 1260 aaaccggacg tatacatcct cggggaaatt tggcatgatg cgatgccgtg gctgcgcggt 1320 gaccagtttg acgcagtcat gaactacccg tttacagacg gggtgctccg ctttttcgcc 1380 aaggaagaga ttggtgcacg ccagtttgct aatcaaatgg tgcatgtact tcattcgtat 1440 ccgaacaatg tcaacgaggc cgcattcaat ttgctcggca gtcatgatac atcaagaatt 1500 ctcaccgttt gcggcgggga tatccgcaag gtgaagttgt tatttttgtt tcaactgacg 1560 ttcacgggtt caccatgcat ttactatggg gatgaaatcg gcatgacggg cggaaacgat 1620 cccgagtgcc ggaagtgcat ggtgtgggat ccgatgcaac aaaacaaaga gctgcaccaa 1680 cacgtcaagc agctgatagc gctgcgcaaa cagtatcggt cactacgccg cggggaaatc 1740 tcctttcttc atgccgatga tgaaatgaac tatcttattt acaaaaaaac agatggagat 1800 gaaacggtgt tagtcatcat caatcggagc gaccaaaaag ccgacatccc gatcccgctc 1860 gatgcaagag gaacatggct cgttaacctc ttgactgggg aacggtttgc agccgaggca 1920 gaaacgcttt gcacctcctt accgccctat gggtttgtac tttatgcaat agaacgttgg 1980 tagacctgtt tcatagacaa tcataatagc agccaatata tgaacaccgg tgcagccgct 2040 gcaccggtgt tatacgcgtt gggctatacg ccgatactca gtcggcgtca tcccttccat 2100 ttttttaaat aaacgcgaga aataagcgct gtcatcaatg ccaactcgtt tggcgatggc 2160 ctcgaccgtt tcatttgttt cagccaacca ttgtttcgcc tttgacaatc ggtagtacgc 2220 caaataatgg ctgcag 2236 // |
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