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EBI DbfetchID AF217414; SV 1; linear; genomic DNA; STD; PRO; 3864 BP. XX AC AF217414; XX DT 23-NOV-2000 (Rel. 65, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Streptococcus pneumoniae pullulanase (spuA) gene, complete cds. XX KW . XX OS Streptococcus pneumoniae OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus. XX RN [1] RP 1-3864 RX DOI; 10.1128/IAI.68.12.7141-7143.2000 RX PUBMED; 11083842. RA Bongaerts R.J., Heinz H.P., Hadding U., Zysk G.; RT "Antigenicity, expression, and molecular characterization of RT surface-located pullulanase of Streptococcus pneumoniae"; RL Infect. Immun. 68(12):7141-7143(2000). XX RN [2] RP 1-3864 RA Bongaerts R.J., Heinz H.P., Zysk G.; RT ; RL Submitted (20-DEC-1999) to the EMBL/GenBank/DDBJ databases. RL Institute of Medical Microbiology and Virology, University of Duesseldorf, RL Universitatsstrasse 1, Duesseldorf, NRW D-40225, Germany XX FH Key Location/Qualifiers FH FT source 1..3864 FT /organism="Streptococcus pneumoniae" FT /strain="3.B" FT /serotype="1" FT /mol_type="genomic DNA" FT /note="clinical isolate" FT /db_xref="taxon:1313" FT CDS 1..3864 FT /codon_start=1 FT /transl_table=11 FT /gene="spuA" FT /product="pullulanase" FT /db_xref="GOA:Q9F930" FT /db_xref="HSSP:1M7X" FT /db_xref="InterPro:IPR019948" FT /db_xref="UniProtKB/TrEMBL:Q9F930" FT /protein_id="AAG33958.1" FT /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISSD FT ESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPATEPTTP FT AASPADPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLW FT TWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDK FT SVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSA FT QWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHS FT QIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL FT GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKL FT GADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNK FT LGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKL FT GPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDL FT GVTHIQLLPVLSYYFVNELKNHEHLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPE FT KRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGG FT RLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIML FT GEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRD FT VNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRR FT LRLGNLMVLTAQGTPFIHSGQEYGRTKQFRNPAYRTPVAEDKVPNKSHLLRDKDGNPFD FT YPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQD FT IKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRN FT AEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTP FT SKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASS FT VKEAVRKESVENSSKENISATPDRQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK FT KEN" XX SQ Sequence 3864 BP; 1297 A; 923 C; 750 G; 894 T; 0 other; atgagaaaaa caccatctca cactgagaaa aaaatggtct acagcatccg ttccctcaaa 60 aatggaactg gttctgtcct tatcggcgca agccttgtct tgcttgccat ggctacacca 120 actatctcat ccgacgaaag tacaccaacc actaacgaac ccaacaacag aaatacaacc 180 acccttgccc aacctcttac tgatacagca gctggctctg gtaagaacga aagtgatatt 240 tcttcacctg gaaatgcaaa cgcttcccta gagaaaacag aagaaaaacc tgcaacagag 300 ccaacaactc ctgctgcaag cccagccgat ccagcaccac aaactggaca agatcgttca 360 agtgagccaa ctacttctac tagtccagta acaactgaaa ctaaggcaga agagcccatc 420 gaagataact acttccgtat ccatgtcaaa aaacttcctg aagaaaacaa ggatgctcaa 480 ggactatgga cttgggacga tgttgaaaaa ccatctgaaa actggccaaa cggagctttg 540 tccttcaagg atgccaagaa agatgactac ggctattacc tagatgtcaa attaaaggga 600 gaacaagcca agaaaattag cttcctcatc aacaatacag ctggaaaaaa tctaaccggc 660 gataaatctg tagaaaaact agttccaaaa atgaacgagg cttggttaga ccaagattac 720 aaggttttct cttacgagcc acagcctgca ggaactgttc gcgtcaacta ctaccgcaca 780 gatggcaact atgacaagaa atctctctgg tactggggag atgtgaaaaa tccaagtagc 840 gctcaatggc ctgacggaac agactttacg gctacaggca aatatggccg ctatatcgac 900 attcctctta atgaagccgc aagagaattt ggatttttat tactagatga gagcaaacaa 960 ggagacgacg tgaaaatccg taaagaaaat tataagttca cagatttgaa aaatcatagc 1020 caaattttcc taaaagacga tgatgaatcg atttacacaa atccatacta tgtccatgat 1080 atccgtatga caggagccca acacgtaggc acttctagca ttgaaagtag cttttcaaca 1140 cttgtcggtg ctaaaaaaga agatatcctc aaacactcca acatcactaa tcacctagga 1200 aacaaggtaa ctattaccga tgttgcaatc gatgaagctg gtaagaaagt gacctacagc 1260 ggagatttct ctgacacaaa acatccttat actgttagct acaattccga ccaattcact 1320 accaaaacaa gctggcgcct gaaagatgag acatacagct atgatggcaa actgggagct 1380 gacctaaaag aagaaggaaa acaagttgat ttgacccttt ggtcaccaag tgctgataag 1440 gtttctgttg ttgtctacga caagaatgac cctgacaaag tagttggaac tgtcgctctc 1500 gaaaaagggg aaagaggaac ttggaaacaa actctagaca gcacaaacaa actcggaatc 1560 acagatttca ctggctacta ttatcaatac caaatcgagc gtcaaggtaa aactgttctt 1620 gcactcgatc cttacgctaa atctcttgct gcttggaata gcgacgatgc caagattgac 1680 gatgcccata aagtggctaa agccgccttt gtagatccag ctaaactcgg acctcaagac 1740 ttgacttatg gtaagattca caatttcaag actcgtgaag acgccgttat ctacgaagct 1800 catgtgcgtg acttcacttc agatcctgcc attgcaaaag acttgaccaa accatttggg 1860 acttttgaag ccttcattga aaaactagac tatctcaaag acttgggtgt aacccatatc 1920 cagctccttc cagtcttgtc ttactacttt gtcaatgaat tgaaaaacca tgaacacttg 1980 tctgactatg cttcaagcaa cagcaactac aactggggat atgaccctca aaactacttc 2040 tccttgactg gtatgtactc aagcgatcct aagaatccag aaaaacgaat cgcagaattt 2100 aaaaacctca tcaacgaaat ccacaaacgt ggtatgggag ctatcctaga tgtcgtttat 2160 aaccacacag ccaaagtcga tatctttgaa gacctagagc caaactacta ccactttatg 2220 gatgccgatg gcacacctcg aactagcttt ggtggtggac gcttggggac aacccaccat 2280 atgaccaaac gcctcctagt tgactctatc aaatacctag ttgataccta caaagtggat 2340 ggattccgtt tcgatatgat gggagaccat gacgccgctt ctatcgaaga agcttacaag 2400 gctgcacgcg ccctcaatcc aaatctaatc atgttagggg aaggttggag aacctatgcc 2460 ggtgatgaaa acatgcctac taaagctgct gaccaagatt ggatgaaaca taccgatact 2520 gtcgctgtct tttcagatga catccgtaac aacctcaaat ctggttatcc aaacgaaggt 2580 caacctgcct ttatcacagg tggcaagcgt gatgttaaca ctatctttaa aaatctcatt 2640 gctcagccaa ctaactttga agctgacagc cctggagatg tcatccaata catcgcagcc 2700 catgataact tgaccctctt tgacatcatt gcccagtcta tcaaaaaaga cccaagcaag 2760 gctgagaact atgctgaaat ccaccgtcgt ttgagacttg gaaatctcat ggtcttgaca 2820 gctcaaggaa ctccgtttat ccactctggc caggaatatg gacgtaccaa acaattccgt 2880 aacccagcct acagaactcc tgtagcagag gataaggttc caaacaaatc tcacttgttg 2940 cgtgataagg acggcaaccc atttgactat ccttacttca tccatgactc ttacgattct 3000 agtgatgcag tcaacaagtt tgactggact aaggctacag atggtaaagc ttatcctgaa 3060 aatgtcaaga gccgtgacta tatgaaaggt ttgattgccc ttcgtcaatc tacagatgcc 3120 ttccgactta agagtcttca agatatcaaa gaccgtgtcc acctcatcac tgtcccaggc 3180 caaaatggtg tggaaaaaga ggatgtagtg attggctatc aaatcactgc tccaaacggc 3240 gatatctacg cagtctttgt caatgcggat gaaaaagctc gcgaatttaa tttgggaact 3300 gcctttgcac atctaagaaa tgcggaagtt ttggcagatg aaaaccaagc aggatcagtc 3360 ggaattgcca acccgaaagg acttgaatgg actgaaaaag gcttgaaatt gaatgccctt 3420 acagctactg ttcttcgagt ctctcaaaat ggaactagcc atgagtcaac tgcagaagag 3480 aaaccagact caaccccttc caagcctgaa catcaaaatg aagcttctca ccctgcacat 3540 caagacccag ctccagaagc tagacctgat tctactaaac cagatgccaa agtagctgat 3600 gcggaaaata aacctagcca agctacagct gattcacaag ctgaacaacc agcacaagaa 3660 gcacaagcat catctgtaaa agaagcggtt cgaaaagaat cggtagaaaa ctctagcaag 3720 gaaaatatat ctgcaacccc agatagacaa gctgaacttc caaatacagg aatcaaaaac 3780 gaaaacaaac tcctatttgc aggaatcagc ctccttgcgc tccttggtct cggtttctta 3840 ctaaaaaata aaaaagagaa ctag 3864 // |
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