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EBI DbfetchID AF217292; SV 1; linear; genomic DNA; STD; PRO; 7512 BP. XX AC AF217292; XX DT 04-OCT-2000 (Rel. 65, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Clostridium difficile cytotoxin B (tcdB) gene, complete cds. XX KW . XX OS Clostridium difficile OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-7512 RX DOI; 10.1128/IAI.68.10.5480-5487.2000 RX PUBMED; 10992443. RA Sambol S.P., Merrigan M.M., Lyerly D., Gerding D.N., Johnson S.; RT "Toxin gene analysis of a variant strain of Clostridium difficile that RT causes human clinical disease"; RL Infect. Immun. 68(10):5480-5487(2000). XX RN [2] RP 1-7512 RA Sambol S.P.; RT ; RL Submitted (16-DEC-1999) to the EMBL/GenBank/DDBJ databases. RL Medicine/Inf Diseases, VA Chicago Healthcare System, Lakeside Division, C/O RL VA/MSB 400 E. Ontario Street, Chicago, IL 60611, USA XX FH Key Location/Qualifiers FH FT source 1..7512 FT /organism="Clostridium difficile" FT /isolate="5340" FT /mol_type="genomic DNA" FT /note="type: CF2" FT /db_xref="taxon:1496" FT CDS 409..7512 FT /codon_start=1 FT /transl_table=11 FT /gene="tcdB" FT /product="cytotoxin B" FT /note="TcdB; variant toxin" FT /db_xref="HSSP:1GVM" FT /db_xref="InterPro:IPR018337" FT /db_xref="UniProtKB/TrEMBL:Q9F931" FT /protein_id="AAG18011.1" FT /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVEK FT YLKLKDINSLTDTYIDTYKKSGRNKALKKFKEYLVIEILELENSNLTPVEKNLHFIWIG FT GQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIIESASNDTLESFRENLND FT PEFNHTAFFRKRMQIIYDKQQNFINYYKAQKEENPDLIIDDIVKTYLSNEYSKDIDELN FT AYIEESLNKVTENSGNDVRNFEEFKTGEVFNLYEQELVERWNLAGASDILRVAILKNIG FT GVYLDVDMLPGIHPDLFKDINKPDSVKTAVDWEEMQLEAIMKHKEYIPEYTSKHFDTLD FT EEVQSSFESVLASKSDKSEIFLPLGDIEVSPLEVKIAFAKGSIINQALISAKDSYCSDL FT LIKQIQNRYKILNDTLGPIISQGNDFNTTMNNFGESLGAIANEENISFIAKIGSYLRVG FT FYPEANTTVTLSGPTIYAGAYKDLLTFKEMSIDTSILSSELRNFEFPKVNISQATEQEK FT NSLWQFNEERAKIQFEEYKKNYFEGALGEDDNLDFSQNTVTDKEYLLEKISSSTKSSER FT GYVHYIVQLQGDKISYEAACNLFAKNPYDSILFQKNIEDSEVAYYYNPTDSEIQEIDKY FT RIPDRISDRPKIKLTFIGHGKAEFNTDIFAGLDVDSLSSEIETAIGLAKEDISPKSIEI FT NLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSMSQDSIIVSANQYEVRINSEGRR FT ELLDHSGEWINKEESIIKDISSKEYISFNPKENKVIVKSKNLPELSTLLQEIRNNSNSS FT DIELEEKVMLAECEINVISNIETQVVEERIEEAKSLTSDSINYIKNEFKLIESISDALC FT DLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETEKTIFSEYANHITEEI FT SKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYNSSKESLSNLSVAMKVQ FT VYAQLFSTGLNTITDAARVVELVSTALDETIDLLPTLSEGLPIIATIIDGVSLGAAIKE FT LSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASGFSILLVPLAGISAGIPSL FT VNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKVMMPQDDLVISEIDFNNNSIVLG FT KCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYDVLEVQKEELDLSKDLMVLPN FT APNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEFYWRYFAFIADALITTLKPRYE FT DTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSGGTYALSLSQYNMGINIELSESD FT VWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIEENKIILNSHEINFSGEVNGSNGF FT VSLTFSILEGINAIIEVDLLSKSYKLLISGELKILMLNSNHIQQKIDYIGFNSELQKNI FT PYSFVDSEGKENGFINGSTKEGLFVSELPDVVLISKVYMDDSKPSFGYYSNNLKDVKVI FT TKDNVNILTGYYLKDDIKISLSLTLQDEKTIKLNSVHLDESGVAEILKFMNRKGSTNTS FT DSLMSFLESMNIKSIFVNFLQSNIKFILDANFIISGTTSIGQFEFICDENNNIQPYFIK FT FNTLETNYTLYVGNRQNMIVEPNYDLDDSGDISSTVINFSQKYLYGIDSCVNKVVISPN FT IYTDEINITPVYETNNTYPEVIVLDANYINEKINVNINDLSIRYVWSNDGNDFILMSTS FT EENKVSQVKIRFVNVFKDKTLANKLSFNFSDKQDVPVSEIILSFTPSYYEDGLIGYDLG FT LVSLYNEKFYINNFGMMVSGLIYINDSLYYFKPPVNNLITGFVTVGDDKYYFNPINGGA FT ASIGETIIDDKNYYFNQSGVLQTGVFSTEDGFKYFAPANTLDENLEGEAIDFTGKLIID FT ENIYYFEDNYRGAVEWKELDGEMHYFSPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVS FT INDNKHYFDDSGVMKVGYTEIDGKHFYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEE FT GEEISYSGILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQY FT YFNDDGIMQVGFVTINDKVFYFSDSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGY FT KYFAPANTVNDNIYGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPE FT TKKACKGINLIDDIKYYFDEKGIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFG FT EDGDMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGS FT VIIDGEEYYFDPDTAQLVISE" XX SQ Sequence 7512 BP; 2981 A; 757 C; 1279 G; 2495 T; 0 other; agacaagctg ttaataaggc taaaaataga gcatttaaaa aaataaaaaa agactatgaa 60 aattatttta acttgtaatt aatgtattta aagaagtatt tacaatagaa atcaaatttt 120 agaattaact ttattataaa atcgataact ttatctaaga atatcttaat ttatatattt 180 tatatacaag aatgtttaca tatttatttc aggcaacatc tttattcaat cgaagagcaa 240 attaatcagc tgagtatctt caatttaaga tgttaggaag tgaatgtata tgaaaaccta 300 agtagatatt agtatatttt atacatagaa aggaggatat ataaaagagt tttagcattt 360 agatgtaaaa atatttaata aaaatattat agtaaaggag aaaattttat gagtttagtt 420 aatagaaaac agttagaaaa aatggcaaat gtaagatttc gtgttcagga agatgaatat 480 gtagcaatat tagatgcatt agaagaatat cataatatgt cagaaaatac tgtagttgaa 540 aagtatctaa aattaaaaga tataaacagt ttaacagata cttatataga tacatataaa 600 aaatctggtc gaaataaagc cttaaaaaaa tttaaagagt acttagttat agagatatta 660 gaattagaaa atagcaattt aactccagtc gagaaaaatt tacattttat atggattgga 720 gggcaaataa atgatactgc tattaattat ataaatcaat ggaaagatgt aaatagtgac 780 tataatgtta atgtttttta tgatagtaat gcatttttga taaacacatt gaaaaaaact 840 ataatagaat cagcatcaaa tgataccctt gaatcattta gagaaaattt aaatgatcct 900 gaatttaacc acactgcatt tttcagaaaa cgtatgcaaa taatctatga taaacagcaa 960 aatttcataa attactataa agctcaaaaa gaagaaaatc ctgaccttat aattgatgat 1020 attgtaaaga catatctttc aaacgagtat tcaaaggata tagatgaact taatgcttat 1080 attgaagagt cattaaacaa agtcacagaa aatagtggaa atgatgttag aaactttgaa 1140 gaatttaaaa ctggagaagt attcaattta tatgaacaag agttagtaga aagatggaat 1200 cttgctggtg catctgatat attaagagtc gctatattga aaaatattgg tggagtctat 1260 ctagatgttg atatgttacc aggaatacac ccagatttat ttaaagatat aaataagcct 1320 gattcagtaa agacagctgt agattgggaa gagatgcagt tagaagccat aatgaaacat 1380 aaagaatata taccagaata tacttcgaaa cattttgata cattggatga agaagttcaa 1440 agtagctttg aatctgtttt agcttctaag tctgataagt cagaaatatt tttaccacta 1500 ggagatatag aggtatcacc tttagaagta aaaattgcat ttgccaaagg ttctattata 1560 aatcaagctc taatttctgc aaaagattca tattgtagtg acttactaat aaaacaaatc 1620 caaaacagat ataagatact gaatgatact ttaggtccaa ttattagtca aggtaatgat 1680 tttaatacta caatgaacaa ttttggtgaa agtttgggag ctatagctaa tgaagagaat 1740 ataagtttta tagcaaaaat cggaagttat ttaagggttg gattttatcc tgaagctaat 1800 actacagtta ctttaagtgg tcctacaata tatgcaggag cttataaaga tttattaaca 1860 tttaaagaga tgagcataga tacttctata ttgtcatctg agttaagaaa ttttgaattt 1920 cctaaggtta atatatctca agcaacagaa caagagaaaa atagtttatg gcaatttaat 1980 gaagaaagag ctaaaattca atttgaagaa tacaagaaaa attattttga aggtgcactt 2040 ggagaagatg ataatcttga tttttctcaa aatacagtaa ctgacaaaga atatctttta 2100 gaaaagatct cttcatcaac gaagagttca gaaagaggat atgttcatta tattgttcaa 2160 ttacaaggag ataaaattag ctatgaagca gcatgtaact tatttgcaaa aaatccttat 2220 gacagtatac tatttcaaaa aaatatagaa gattcagaag tagcatatta ctataatcct 2280 acagatagtg aaatacaaga aattgataag tatagaattc ctgatagaat ctctgataga 2340 cctaagatta aattaacatt cattggtcat ggcaaagctg aatttaatac tgatatattt 2400 gcaggtcttg atgtagattc attatcttca gaaatagaaa cagcaatagg tttagccaaa 2460 gaggatattt ctcctaaatc tatagaaata aacttactgg gatgtaacat gtttagctat 2520 tctgtaaatg tagaagagac ttatcctggg aaattattac ttagagttaa agataaagta 2580 tcagaattaa tgccatctat gagtcaagac tctattatag taagtgcaaa tcaatatgaa 2640 gttagaataa atagtgaagg aagaagagaa ttattagacc attctggtga atggataaac 2700 aaagaagaaa gtattataaa ggatatttca tcaaaagaat atatatcatt taatcctaaa 2760 gagaataaag ttatagtaaa atctaaaaat ttacctgaat tatctacatt attacaagaa 2820 attagaaata attctaattc aagtgatatt gaactagaag aaaaagtaat gttagcagaa 2880 tgtgagataa atgttatttc aaatatagag acacaagtgg tagaagaaag aattgaagaa 2940 gctaaaagct taacttctga ctctattaat tatataaaga atgaatttaa actaatagaa 3000 tctatttctg atgcactatg tgacttaaaa caacagaatg aattagaaga ttctcatttt 3060 atatcttttg aggacatatc agagactgat gaggggttta gtataagatt tattaataaa 3120 gaaactggag aatctatatt tgtagaaact gaaaaaacaa tattctctga atatgctaat 3180 catataactg aagagatttc taagataaaa ggtactatat ttgatactgt aaatggtaag 3240 ttagtaaaaa aagtaaattt agatactaca cacgaagtaa atactttaaa tgctgcattt 3300 tttatacaat cattaataga atataatagt tctaaagaat ctcttagtaa tttaagtgta 3360 gcaatgaaag ttcaagttta cgctcaatta tttagtactg gtttaaatac tattacagat 3420 gcagccagag ttgttgaatt agtatcaact gcattagatg aaactataga cttacttcct 3480 acattatctg aaggattacc tataattgca actattatag atggtgtaag tttaggtgca 3540 gcaatcaaag agctaagtga aacgagtgac ccattattaa gacaagaaat agaagctaag 3600 ataggtataa tggcagtaaa tttaacaaca gctacaactg caatcattac ttcatctttg 3660 gggatagcta gtggatttag tatactttta gttcctttag caggaatttc agcaggtata 3720 ccaagcttag taaacaatga acttgtactt cgagataagg caacaaaggt tgtagattat 3780 tttaaacatg tttcattagt tgaaactgaa ggagtattta ctttattaga tgataaagta 3840 atgatgccac aagatgattt agtgatatca gaaatagatt ttaataataa ttcaatagtt 3900 ttaggtaaat gtgaaatctg gagaatggaa ggtggttcag gtcatactgt aactgatgat 3960 atagatcact tcttttcagc accatcaata acatatagag agccacactt atctatatat 4020 gacgtattgg aagtacaaaa agaagaactt gatttgtcaa aagatttaat ggtattacct 4080 aatgctccaa atagagtatt tgcttgggaa acaggatgga caccaggttt aagaagctta 4140 gaaaatgatg gcacaaaact gttagaccgt ataagagata actatgaagg tgagttttat 4200 tggagatatt ttgcttttat agctgatgct ttaataacaa cattaaaacc aagatatgaa 4260 gatactaata taagaataaa tttagatagt aatactagaa gttttatagt tccaataata 4320 actacagaat atataagaga aaaattatca tattctttct atggttcagg aggaacttat 4380 gcattgtctc tttctcaata taatatgggt ataaatatag aattaagtga aagtgatgtt 4440 tggattatag atgttgataa tgttgtgaga gatgtaacta tagaatctga taaaattaaa 4500 aaaggtgatt taatagaagg tattttatct acactaagta ttgaagagaa taaaattatc 4560 ttaaatagcc atgagattaa tttttctggt gaggtaaatg gaagtaatgg atttgtttct 4620 ttaacatttt caattttaga aggaataaat gcaattatag aagttgattt attatctaaa 4680 tcatataaat tacttatttc tggcgaatta aaaatattga tgttaaattc aaatcatatt 4740 caacagaaaa tagattatat aggattcaat agcgaattac agaaaaatat accatatagc 4800 tttgtagata gtgaaggaaa agagaatggt tttattaatg gttcaacaaa agaaggttta 4860 tttgtatctg aattacctga tgtagttctt ataagtaagg tttatatgga tgatagtaag 4920 ccttcatttg gatattatag taataatttg aaagatgtca aagttataac taaagataat 4980 gttaatatat taacaggtta ttatcttaag gatgatataa aaatctctct ttctttgact 5040 ctacaagatg aaaaaactat aaagttaaat agtgtgcatt tagatgaaag tggagtagct 5100 gagattttga agttcatgaa tagaaaaggt agtacaaata cttcagattc tttaatgagc 5160 tttttagaaa gtatgaatat aaaaagtatt ttcgttaatt tcttacaatc taatattaag 5220 tttatattag atgctaattt tataataagt ggtactactt ctattggcca atttgagttt 5280 atttgtgatg aaaataataa tatacaacca tatttcatta agtttaatac actagaaact 5340 aattatactt tatatgtagg aaatagacaa aatatgatag tggaaccaaa ttatgattta 5400 gatgattctg gagatatatc ttcaactgtt atcaatttct ctcaaaagta tctttatgga 5460 atagacagtt gtgttaataa agttgtaatt tcaccaaata tttatacaga tgaaataaat 5520 ataacgcctg tatatgaaac aaataatact tatccagaag ttattgtatt agatgcaaat 5580 tatataaacg aaaaaataaa tgttaatatc aatgatctat ctatacgata tgtatggagt 5640 aatgatggta atgattttat tcttatgtca actagtgaag aaaataaggt gtcacaagtt 5700 aaaataagat tcgttaatgt ttttaaagat aagactttgg caaataagct atcttttaac 5760 tttagtgata aacaagatgt acctgtaagt gaaataatct tatcatttac accttcatat 5820 tatgaggatg gattgattgg ctatgatttg ggtctagttt ctttatataa tgagaaattt 5880 tatattaata actttggaat gatggtatct ggattaatat atattaatga ttcattatat 5940 tattttaaac caccagtaaa taatttgata actggatttg tgactgtagg cgatgataaa 6000 tactacttta atccaattaa tggtggagct gcttcaattg gagagacaat aattgatgac 6060 aaaaattatt atttcaacca aagtggagtg ttacaaacag gtgtatttag tacagaagat 6120 ggatttaaat attttgcccc agctaataca cttgatgaaa acctagaagg agaagcaatt 6180 gattttactg gaaaattaat tattgacgaa aatatttatt attttgaaga taattataga 6240 ggagctgtag aatggaaaga attagatggt gaaatgcact attttagccc agaaacaggt 6300 aaagctttta aaggtctaaa tcaaataggt gatgataaat actatttcaa ttctgatgga 6360 gttatgcaaa aaggatttgt tagtataaat gataataaac actattttga tgattctggt 6420 gttatgaaag taggttacac tgaaatagat ggcaagcatt tctactttgc tgaaaacgga 6480 gaaatgcaaa taggagtatt taatacagaa gatggattta aatattttgc tcatcataat 6540 gaagatttag gaaatgaaga aggtgaagaa atctcatatt ctggtatatt aaatttcaat 6600 aataaaattt actattttga tgattcattt acagctgtag ttggatggaa agatttagag 6660 gatggttcaa agtattattt tgatgaagat acagcagaag catatatagg tttgtcatta 6720 ataaatgatg gtcaatatta ttttaatgat gatggaatta tgcaagttgg atttgtcact 6780 ataaatgata aagtcttcta cttctctgac tctggaatta tagaatctgg agtacaaaac 6840 atagatgaca attatttcta tatagatgat aatggtatag ttcaaattgg tgtatttgat 6900 acttcagatg gatataaata ttttgcacct gctaatactg taaatgataa tatttacgga 6960 caagcagttg aatatagtgg tttagttaga gttggtgaag atgtatatta ttttggagaa 7020 acatatacaa ttgagactgg atggatatat gatatggaaa atgaaagtga taaatattat 7080 ttcgatccag aaactaaaaa agcatgcaaa ggtattaatt taattgatga tataaaatat 7140 tattttgatg agaagggcat aatgagaacg ggtcttatat catttgaaaa taataattat 7200 tactttaatg agaatggtga aatgcaattt ggttatataa atatagaaga taagatgttc 7260 tattttggtg aagatggtga catgcagatt ggagtattta atacaccaga tggatttaaa 7320 tactttgcac atcaaaatac tttggatgag aattttgagg gagaatcaat aaactatact 7380 ggttggttag atttagatga aaagagatat tattttacag atgaatatat tgcagcaact 7440 ggttcagtta ttattgatgg tgaggagtat tattttgatc ctgatacagc tcaattagtg 7500 attagtgaat ag 7512 // |
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