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EBI DbfetchID AF203780; SV 1; linear; mRNA; STD; INV; 2280 BP. XX AC AF203780; XX DT 01-NOV-2001 (Rel. 69, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Brevicoryne brassicae thioglucosidase (BMY1) mRNA, complete cds. XX KW . XX OS Brevicoryne brassicae (cabbage aphid) OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; OC Paraneoptera; Hemiptera; Sternorrhyncha; Aphidiformes; Aphidoidea; OC Aphididae; Macrosiphini; Brevicoryne. XX RN [1] RC Patent: GB (0020331.5)-A 17-AUG-2000; Imperial College Innovations RP 1-2280 RA Jones A.E., Winge P., Rossiter J.T.; RT "The Enzyme"; RL Unpublished. XX RN [2] RP 1-2280 RX DOI; 10.1016/S0965-1748(01)00088-1 RX PUBMED; 11804799. RA Jones A.M., Winge P., Bones A.M., Cole R., Rossiter J.T.; RT "Characterization and evolution of a myrosinase from the cabbage aphid RT Brevicoryne brassicae"; RL Insect Biochem. Mol. Biol. 32(3):275-284(2002). XX RN [3] RP 1-2280 RA Jones A.E., Winge P., Rossiter J.T.; RT ; RL Submitted (11-NOV-1999) to the EMBL/GenBank/DDBJ databases. RL genetics, ITE Furzebrook, Wareham Road, Wareham, Dorset BH20 5AS, England XX FH Key Location/Qualifiers FH FT source 1..2280 FT /organism="Brevicoryne brassicae" FT /mol_type="mRNA" FT /db_xref="taxon:69196" FT CDS 235..1629 FT /codon_start=1 FT /gene="BMY1" FT /product="thioglucosidase" FT /function="hydrolyses glucosinolates" FT /note="myrosinase" FT /db_xref="GOA:Q95X01" FT /db_xref="InterPro:IPR001360" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="InterPro:IPR018120" FT /db_xref="PDB:1WCG" FT /db_xref="UniProtKB/TrEMBL:Q95X01" FT /protein_id="AAL25999.1" FT /translation="MDYKFPKDFMFGTSTASYQIEGGWNEDGKGENIWDRLVHTSPEVI FT KDGTNGDIACDSYHKYKEDVAIIKDLNLKFYRFSISWARIAPSGVMNSLEPKGIAYYNN FT LINELIKNDIIPLVTMYHWDLPQYLQDLGGWVNPIMSDYFKEYARVLFTYFGDRVKWWI FT TFNEPIAVCKGYSIKAYAPNLNLKTTGHYLAGHTQLIAHGKAYRLYEEMFKPTQNGKIS FT ISISGVFFMPKNAESDDDIETAERANQFERGWFGHPVYKGDYPPIMKKWVDQKSKEEGL FT PWSKLPKFTKDEIKLLKGTADFYALNHYSSRLVTFGSDPNPNFNPDASYVTSVDEAWLK FT PNETPYIIPVPEGLRKLLIWLKNEYGNPQLLITENGYGDDGQLDDFEKISYLKNYLNAT FT LQAMYEDKCNVIGYTVWSLLDNFEWFYGYSIHFGLVKIDFNDPQRTRTKRESYTYFKNV FT VSTGKP" FT misc_feature 247..1617 FT /gene="BMY1" FT /note="Glyco_hydro_1; CDD pfam00232; Region: Glycosyl FT hydrolase family 1" XX SQ Sequence 2280 BP; 848 A; 372 C; 372 G; 684 T; 4 other; atctcgctag tttacgctat tctagttaaa ctctgttcaa atttatcggt gaactttata 60 agttaaatgt attaatgtat ttagacattg ttgaattata acacgaatat tcaaacgctt 120 tggttaatta tttcaaaaat tcttccatct catcaaacgg tttggactcg cgacaatcaa 180 taccaagttt ctcattgaac taaactcgac aattaatcaa tttaagtatt caatatggat 240 tataaatttc caaaggattt tatgtttggc acttcaactg cctcatatca aattgaagga 300 ggctggaatg aagacggaaa aggagaaaat atttgggatc gtttggttca tactagtcca 360 gaagtaataa aagatgggac taatggagat attgcctgtg attcctatca caagtataaa 420 gaagatgtag caattataaa agatttgaat ttgaagtttt atcgtttttc aatatcatgg 480 gctcgaatag caccatctgg agtaatgaat tcattagaac caaaaggaat agcatactat 540 aataatttaa tcaatgaact tatcaagaat gatattattc ctttagttac gatgtatcat 600 tgggacttac cacaatacct acaggatctt ggaggttggg ttaatccaat aatgtcagat 660 tattttaaag aatatgcacg agtgttattt acttacttcg gagacagagt aaaatggtgg 720 ataacattta atgaaccaat agctgtttgt aaaggttatt ccattaaagc ctatgctcca 780 aacttgaatt taaagaccac cggacattat ttagcaggtc atacacaact tattgcacat 840 ggaaaagcat ataggttgta tgaagaaatg tttaaaccta cacaaaatgg aaaaataagt 900 atttcaatta gtggagtgtt tttcatgcca aaaaatgctg aatcagatga tgatatagaa 960 actgctgaaa gagctaacca atttgagaga ggatggttcg gtcatccagt gtacaaggga 1020 gactatccac ctataatgaa aaaatgggtt gatcaaaaga gtaaagaaga aggtttacca 1080 tggtccaaat tacctaaatt tacaaaagat gaaataaaat tacttaaagg tactgctgat 1140 ttttatgctc tcaatcatta ttcgtctcgt ttggtgactt ttggaagtga tccaaatcct 1200 aattttaatc ctgacgcatc ttatgttact tctgtagacg aagcatggtt aaagccgaat 1260 gaaacaccgt atattatacc agtacccgaa ggtttaagaa aacttttgat atggttaaaa 1320 aacgaatatg gcaatcccca attgcttatt acagaaaatg gatatggaga cgacggtcaa 1380 ttggatgatt ttgaaaaaat tagctaccta aagaactatt taaatgcaac attacaagcg 1440 atgtatgaag ataaatgcaa tgtaatagga tataccgtgt ggtcactctt ggacaatttt 1500 gaatggtttt atggttattc gattcatttt ggacttgtta agatagattt taatgaccct 1560 caaagaactc gtactaaaag agaatcatac acatatttca agaatgtggt atcaactggc 1620 aaaccataat atttataaac accttcgatt agttaatatt agaaaaacgc ttttatccga 1680 attatgaaaa atgtaatttt aattaaataa caataaacat atacacataa tatacataac 1740 atttcacaat caactctcaa cgcgataaca ccgaactaaa tctatcgact aacatctata 1800 aacaggtact aagctggcca gccgtgcacc gactacataa tgagccattt atacttgtaa 1860 cggttacatc actcaccaaa acattctttc taaggactac acaatcaacc aatcagaaca 1920 tgacatacag gaaataagag cagtcagaga cttttgatca aatcttaatt ctctggacta 1980 tactaatcaa cccgatactt acgaacatgg ataggggagg tcaggacaaa gataatgaga 2040 cccttcacat ggccgtaacc cagaaatcca gcaaacagca tccagatccg ttcagaaaca 2100 aaaacaacac ggcggtacac ccgatagttt actagtcggt acagcacgcg gattataccc 2160 tttattttct tcaatataac attattatat agctaaataa ctatgtattg cttttttttt 2220 tnaaataaaa ttttntgaac cntcntttta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2280 // |
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