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EBI DbfetchID AF195244; SV 1; linear; genomic DNA; STD; PRO; 5762 BP. XX AC AF195244; XX DT 02-NOV-2000 (Rel. 65, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Pyrococcus furiosus galactokinase (galK), alpha-galactosidase (galA), DE putative transporter (mfsA), beta-glucosidase (bglA), and putative DE galactose-1-phosphate uridylyltransferase (galT) genes, complete cds. XX KW . XX OS Pyrococcus furiosus OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Pyrococcus. XX RN [1] RP 1-5762 RA Verhees C.H.; RT ; RL Submitted (15-OCT-1999) to the EMBL/GenBank/DDBJ databases. RL Laboratory of Microbiology, Wageningen University and Research Center, RL Hesselink van Suchtelenweg 4, Wageningen NL-6703 CT, The Netherlands XX FH Key Location/Qualifiers FH FT source 1..5762 FT /organism="Pyrococcus furiosus" FT /strain="DSM 3638" FT /mol_type="genomic DNA" FT /db_xref="taxon:2261" FT CDS 1..1059 FT /codon_start=1 FT /transl_table=11 FT /gene="galK" FT /product="galactokinase" FT /note="highly specific ATP-dependent galactokinase" FT /db_xref="GOA:Q9HHB6" FT /db_xref="InterPro:IPR014721" FT /db_xref="PDB:1S4E" FT /db_xref="UniProtKB/Swiss-Prot:Q9HHB6" FT /protein_id="AAG28454.1" FT /translation="MSKITVKSPGRVNLIGEHTDYTYGYVMPMAIDLYTIITAEKYDKV FT QLYSEHFNEEKTFTLDNLTKEGSWIDYVKGVLWVLIQEGYKIGGLKGKITGDLPLGAGL FT SSSASFEVGILEVLNQLYNLNIDPLKKALLAKKAENEFVGVPCGILDQFAVVFGKKDNV FT IFLDTQTLQYEYIPFPKDVSVLVFYTGVKRELASSEYAERKRIAEESLRILGKESSKEV FT TEKDLGKLPPLHRKFFSYIVRENARVLEVRDALKEGDIEKVGKILTTAHWDLAENYRVS FT CEELDFFVKKAMELGAYGARLTGAGFGGSAIALVDKDKAKTIGDAILREYLAKFSWKAK FT YFVVKPSDGVGV" FT CDS 1056..2150 FT /codon_start=1 FT /transl_table=11 FT /gene="galA" FT /product="alpha-galactosidase" FT /note="family 57 glycosyl hydrolase" FT /db_xref="GOA:Q9HHB5" FT /db_xref="InterPro:IPR004300" FT /db_xref="UniProtKB/TrEMBL:Q9HHB5" FT /protein_id="AAG28455.1" FT /translation="MRALVFHGNLQYAEIPKSEIPKVIEKAYFPTISELIRREIPFGLN FT ITGYSLSFLPKDLIALIKEGIESGLIEILGTSYTHAILPLLPLSRVEAQIKRDREVKEN FT ILEVSPEGFWLPELAYDPIIPAILRDNNYEYLFADGEAMLFSNHLNSAIKPIKPLYPHL FT IKAQRGEGLVYLNYLLGLRELKKAINLVFEGKVTLEAVKEIEAIPVWVSINTAVMLGAG FT RFPLMNPKKVAKWVKEKDEILLYGTDIEFLGYRDIAGYKITISNLLEIINELEGELGLP FT RKIKHSEKKLYLRTSSWAPDKSLRIWTEDEGNARLNMLTSYMDGELAFLAENSDARGWE FT PLPERRLDAFKAIYTHWRSENGKH" FT CDS 2137..3468 FT /codon_start=1 FT /transl_table=11 FT /gene="mfsA" FT /product="putative transporter" FT /note="similar to major facilitator super-family secondary FT transport proteins (symport/antiport)" FT /db_xref="InterPro:IPR011701" FT /db_xref="UniProtKB/TrEMBL:Q9HHB4" FT /protein_id="AAG28456.1" FT /translation="MGNIRRRVSIVLLILMAAFLMADQNLLPPNYQQIMKEFGIGETQM FT GLVSSIFVATSALITIIWGMLADIKQRKKLLVIGVLLGEIPCFLTAYVQTYWQLLAMRF FT LTGIGIGSIIPIGYSLIADMFEEEKRGRGYSYIETAFGFGTLFGMIIAGMIASWRTPFI FT IAAVPNFILAPLFYIIAEEPKRGAGEKEIRALIEKGYEYTYRLNLEAIRKSFQTKTNIL FT IFLQGIIGTVPWGILLYWLVSFLQVTRGMDKSTATFVLLIIGIASVFGSLLGGFVGDYF FT EAKRRGGRATITGIAIFLGMLASIGLILYPLPSKLSTVHWIGLAIYSILFIQFVSYAGP FT NVRAIVSQVNLPEDRGTVFGLFNILDNVGKAIGPLFGGFLIETLKSMGYMEPLAYQYTL FT LIGALFWIPCALVWIWIRKSYPEDRDKVKEILKKRAEEILKSKT" FT CDS complement(3457..4722) FT /codon_start=1 FT /transl_table=11 FT /gene="bglA" FT /product="beta-glucosidase" FT /note="family 1 glycosyl hydrolase" FT /db_xref="GOA:Q9HHB3" FT /db_xref="HSSP:1GNX" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9HHB3" FT /protein_id="AAG28457.1" FT /translation="MPLKFPEEFLFGTATAAHQIEGDNKWNDWWYYEQIGKLPYKSGKA FT CNHWEFYKEDIQLMASLGYNAYRFSIEWSRLFPEENKFNEEAFNRYQEIIDLLLANNIT FT PLVTLHHFTSPLWFMKKGGFLREENLKFWEKYVEKVAELLEKVKLIATFNEPMVYVMMG FT YLTAYWPPFIKSPFKAFKVASNLLKAHALAYEILHGKFQVGIVKNVPIMLPATDKERDK FT KAAERADNLFNWYFLDAIWSGVYRGAFKAYRVPQSDADFIGINYYTASEVRHSWNPLKF FT FFDAKLADVSERKTQMGWSVYPRGIYIALKKASKYGKPLYITENGIATLDDEWRIEFII FT QHLQYVHKAIEDGLDVRGYFYWSFMDNYEWREGFEPRFGLVEVDYETFERRPRKSAYIY FT GGIAKSKEIKDEILEKYGLSSL" FT CDS 4779..5762 FT /codon_start=1 FT /transl_table=11 FT /gene="galT" FT /product="putative galactose-1-phosphate FT uridylyltransferase" FT /db_xref="GOA:Q9HHB2" FT /db_xref="HSSP:1HXP" FT /db_xref="InterPro:IPR013838" FT /db_xref="UniProtKB/TrEMBL:Q9HHB2" FT /protein_id="AAG28458.1" FT /translation="MRELRYNPLTGQWIMVSAVRKKRPWRPTNFCPFCPGSEETGYGWK FT VLLLPNRFPMLSFDAPKPESEEFYKKARAIGQCSVIVETPDHDLKDLDELPREQMVKVV FT ELWVEATKTLKENPHVAYLAIFRNKGEEIGVSLTHPHGQLYALPYIPLKARLKIENSRK FT YYKSKNECLFCRILKEEKESEERIIYENDNFIVFMPFFASWPFEMHIYPKRHVQYLTDL FT NKEEIESLADAIQATTGTLNTILERQMPYTMMIFQAPFKGNYKFYHLHIEFYPILRDNG FT KIKYAAGIEMGTWEFTYDAIPEENARKLKEACKKVSQRLNLKGKCI" XX SQ Sequence 5762 BP; 1896 A; 1123 C; 1174 G; 1569 T; 0 other; atgagtaaaa tcactgtaaa atctcctggt agggttaact tgatcggaga gcacaccgat 60 tacacctatg gttatgtaat gccaatggca atcgatctct acacaataat aacagccgaa 120 aaatacgata aagtccagct atactccgaa cacttcaacg aagagaaaac atttacatta 180 gataatctca caaaggaagg atcatggata gactacgtaa aaggagtatt gtgggtttta 240 atccaagaag ggtacaaaat tggaggacta aaaggaaaaa taacagggga ccttcccctc 300 ggagctggtc taagttcctc agcaagcttt gaagttggaa ttttagaagt gctaaatcaa 360 ctatataatt taaatatcga tccactcaaa aaggcattac ttgcgaaaaa agctgaaaat 420 gaatttgtgg gagttccatg cggaatctta gatcaatttg cagttgtttt tggaaaaaag 480 gacaatgtta tatttctaga tacacaaaca ctgcaatatg aatatattcc ttttcccaaa 540 gacgtttcag tgttggtatt ttatactgga gtaaagagag agttagcatc ttcagaatac 600 gcagaaagaa aacgcatagc agaagaaagt ctgagaattc ttgggaaaga gtcctcaaaa 660 gaggtgacag aaaaggactt gggcaaactt cctcccctac acaggaagtt cttcagctat 720 atagttagag aaaacgctag agttcttgaa gttagagatg cacttaaaga gggggacata 780 gaaaaggtag gaaaaattct cacaacagca cattgggatc tagcggagaa ttatagagtc 840 agctgcgaag agttagactt ttttgttaaa aaagcgatgg aattaggcgc ttatggggcc 900 aggctaacag gcgctggatt tggtggctcg gcaatagcac tagttgataa agataaggca 960 aaaacaatag gagatgcaat attgagggaa tatctcgcga aattttcatg gaaagctaaa 1020 tatttcgtcg taaaaccctc cgatggtgtg ggagtatgag agcactagtc tttcatggga 1080 atttacaata tgcagaaata ccaaagagcg aaattccaaa ggttatagaa aaagcatact 1140 tccccactat ttcagaactc ataagaagag agataccctt tggccttaac ataacgggat 1200 actcattaag ctttctccca aaagatttga ttgccctcat aaaggaggga atagagagtg 1260 gcctcataga gattcttgga acaagttata ctcacgcaat tctcccactt ctcccactct 1320 ctagagttga ggcacaaata aaaagagata gagaagtgaa agagaacata ttggaagtct 1380 ccccagaagg attctggctt cccgaattgg catatgaccc aataattcca gcaattttga 1440 gggacaacaa ctatgaatat ctcttcgctg acggcgaggc tatgctattc tctaaccatc 1500 taaactctgc aataaaacca attaaaccct tataccctca tctaataaaa gcccaaagag 1560 gagaggggtt agtgtactta aattaccttt taggccttag agagctcaaa aaggccataa 1620 atcttgtctt tgaaggaaaa gtcacactag aagcagtcaa agaaatcgaa gctatcccgg 1680 tatgggtttc catcaatact gcagtaatgc tgggagcggg aagatttcca cttatgaatc 1740 ctaaaaaagt cgcaaaatgg gtaaaggaaa aagatgaaat tttgctttat ggaacagaca 1800 tagagttcct cggatacaga gatatagcag ggtataagat tacaatttcc aacctattgg 1860 agattataaa tgagcttgaa ggagaattag gtctacccag gaagataaag catagtgaaa 1920 aaaagctata tctaagaacc tcaagttggg ctccggacaa aagtttgaga atttggacag 1980 aggatgaagg gaatgcaaga ttaaacatgc ttacctccta tatggatgga gaacttgcat 2040 ttttagctga gaatagtgat gcccgaggtt gggaacctct tcccgaaaga aggttagacg 2100 catttaaagc tatatacacc cattggagga gtgaaaatgg gaaacattag aagaagggtt 2160 tccatagttc ttctgatatt gatggccgct tttttaatgg ctgaccaaaa cttgctacct 2220 ccaaactacc agcaaatcat gaaagagttc ggcatagggg aaactcaaat gggacttgtt 2280 tcctcgatat ttgtggccac tagcgcctta attaccataa tctggggtat gttagctgat 2340 ataaagcaaa gaaaaaaatt attggtcatt ggagtcctct taggagaaat tccttgcttc 2400 cttactgcat acgttcagac atactggcaa cttctagcaa tgaggttcct cacgggcatt 2460 ggaattggct caataattcc aatagggtat tcactgattg cagacatgtt tgaagaagaa 2520 aagagaggaa ggggttattc gtacatagaa acagcttttg gatttggaac attatttgga 2580 atgataattg ctgggatgat agccagctgg agaaccccat tcatcattgc agcagttcca 2640 aactttatac ttgctcccct attttatata atagctgagg aaccaaagag aggggcagga 2700 gaaaaggaga ttagagctct tattgaaaag ggatacgagt acacctacag attgaatctt 2760 gaggcaataa gaaaatcatt ccaaacaaaa acaaacattc taattttcct ccaaggaatt 2820 ataggaacag ttccatgggg gatacttctc tattggcttg tttccttcct acaggttaca 2880 agaggaatgg acaagtcaac agcaacattc gtacttctaa taatcggaat agccagtgtt 2940 ttcggtagcc tgcttggtgg attcgtagga gattactttg aggctaagag aagaggagga 3000 agagccacta taacaggaat tgccattttc ctgggaatgt tagcctcaat aggcctaata 3060 ctttacccac ttccaagtaa actcagcaca gttcattgga tcggcctagc gatttactca 3120 attctattca tccaatttgt ctcttacgca ggaccaaacg tgagggccat tgtctcccag 3180 gtcaatctac cagaagacag ggggacagtt ttcggactct ttaacatact tgataatgtt 3240 ggaaaggcaa tcggaccact atttggagga ttccttatag aaactctcaa gagtatggga 3300 tacatggaac cattagcata ccaatacacc ctcttaattg gagcattgtt ctggataccc 3360 tgtgcactag tctggatttg gataagaaag agctacccag aagacaggga taaagttaaa 3420 gaaatattaa agaagagagc agaagaaatt ttaaagtcaa agacttgaca atccatattt 3480 ttccaatatt tcatctttta tttccttact ttttgcaatc cccccatata tgtaggcact 3540 cttcctaggc cttctttcaa aggtctcata atccacttcc accaacccaa acctaggttc 3600 aaagccttct ctccattcat agttgtccat aaatgaccaa tagaagtagc ctcttacatc 3660 caagccatct tcaatggcct tatgaacgta ttggagatgt tggattatga actctattct 3720 ccactcatca tctagcgttg ctattccgtt ctcggtaatg tatagtggtt ttccatactt 3780 agaggccttt tttagagcta tgtatattcc cctgggataa acactccatc ccatctgggt 3840 ctttctctca ctaacatccg ccaatttcgc gtcaaagaaa aactttagcg gattccagct 3900 atgcctcact tcacttgctg tataataatt tatcccaatg aagtctgcgt cgctttgtgg 3960 aaccctataa gccttgaatg ctcccctgta caccccactc caaattgcat ccaagaagta 4020 ccaattgaac aaattatcag ctctctcagc ggcttttttg tccctctctt tgtcagtagc 4080 tgggagcatt attggaacgt ttttcactat tcctacttga aacttcccat gaagaatttc 4140 atatgcaagc gcatgagctt tgagcaagtt tgaagcgact ttaaaggctt taaagggact 4200 tttgatgaag ggaggccaat aggcagttag ataacccatc atcacatata ccattggctc 4260 gttgaatgtt gctatcaatt ttactttttc taagagctct gcaacctttt ccacatactt 4320 ttcccagaac tttaagttct cctctctaag aaagcctcct ttcttcatga accagagcgg 4380 actagtaaag tgatggagag tcactagagg agttatgttg ttcgctaaaa gcaggtctat 4440 aatttcttga tatctattga aagcttcttc attaaatttg ttctcttctg ggaacaatct 4500 gctccactct atggagaatc tataggcatt gtaccccaaa cttgccatta gctggatatc 4560 ctctttatag aattcccaat ggttacacgc cttcccagac ttatagggga gtttgccaat 4620 ctgctcataa taccaccaat cattccactt gttgtctccc tctatttggt gagccgctgt 4680 tgcagtacca aacaggaact cttcaggaaa cttaaggggc atttgaaacc accaaataca 4740 ttttaaaaag ataagatatt ttaaagtttg gtggaaagat gagggagctg agatataacc 4800 ccctcacagg tcagtggatc atggtgtctg cagttaggaa aaaaagacca tggagaccca 4860 caaatttctg tccattttgc ccaggaagcg aagagacagg ttatggatgg aaagttcttt 4920 tgcttccaaa tagattccca atgttatctt ttgatgcccc aaaacctgag agtgaggaat 4980 tttacaagaa agctagggca attggccagt gtagtgttat cgtcgagaca ccagatcatg 5040 atcttaaaga tctagacgag cttcccaggg agcaaatggt taaggttgtt gaactgtggg 5100 tagaggctac gaaaacacta aaggaaaatc cccacgttgc ttatctcgcg atatttagaa 5160 ataaagggga ggaaattgga gtaagcttaa ctcatcccca cggccagctt tatgcccttc 5220 cgtatattcc tctcaaagct agactcaaaa tagaaaactc tagaaagtac tacaagagca 5280 aaaatgaatg tttgttctgt aggatactta aagaagaaaa agaaagtgaa gagagaatta 5340 tctacgaaaa tgacaacttt atagttttca tgcccttctt tgcaagttgg cctttcgaaa 5400 tgcacattta tccaaaaaga cacgttcagt atttaacaga cctcaacaag gaagaaatag 5460 aaagcttggc ggatgcaatt caagctacca ctggaacctt aaacaccatt ttagaaaggc 5520 aaatgcccta cacaatgatg atattccagg ctccatttaa aggaaactac aagttttatc 5580 acctacacat agagttctac cctattttaa gagacaatgg aaagatcaaa tacgcagcag 5640 gaattgaaat gggtacatgg gaatttacat acgatgcaat cccagaagaa aacgccagaa 5700 agcttaaaga ggcatgcaaa aaagtttcac aaagacttaa ccttaaaggg aaatgtattt 5760 aa 5762 // |
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