EBI Dbfetch

ID   AF181032; SV 1; linear; genomic DNA; STD; PRO; 1134 BP.
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AC   AF181032;
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DT   13-SEP-1999 (Rel. 61, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 3)
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DE   Pyrococcus furiosus endoglucanase A precursor (eglA) gene, complete cds.
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KW   .
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OS   Pyrococcus furiosus
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
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RN   [1]
RC   Patent pending
RP   1-1134
RA   Bylina E.J., Swanson R.V., Mathur E.J., Lam D.E., Callen W.;
RT   "Glycosidases";
RL   Unpublished.
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RN   [2]
RP   1-1134
RX   PUBMED; 9864341.
RA   Bauer M.W., Driskill L.E., Callen W., Snead M.A., Mathur E.J., Kelly R.M.;
RT   "An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus
RT   furiosus hydrolyzes beta-1,4 bonds in mixed-linkage
RT   (1-->3),(1-->4)-beta-D-glucans and cellulose";
RL   J. Bacteriol. 181(1):284-290(1999).
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RN   [3]
RP   1-1134
RA   Callen W., Snead M.A., Mathur E.J.;
RT   ;
RL   Submitted (25-AUG-1999) to the EMBL/GenBank/DDBJ databases.
RL   Diversa Corporation, 10665 Sorrento Valley Road, San Diego, CA 92121, USA
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RN   [4]
RP   1-1134
RA   Bauer M.W., Driskill L.E., Kelly R.M.;
RT   ;
RL   Submitted (25-AUG-1999) to the EMBL/GenBank/DDBJ databases.
RL   Chemical Engineering, North Carolina State University, P. O. Box 7905,
RL   Raleigh, NC 27695, USA
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FH   Key             Location/Qualifiers
FH
FT   source          1..1134
FT                   /organism="Pyrococcus furiosus"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:2261"
FT   CDS             82..1041
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="eglA"
FT                   /product="endoglucanase A precursor"
FT                   /note="beta D-(1, 4)-endoglucanase precursor; corrected
FT                   version of published sequence"
FT                   /db_xref="GOA:Q9V2T0"
FT                   /db_xref="InterPro:IPR002594"
FT                   /db_xref="InterPro:IPR008985"
FT                   /db_xref="InterPro:IPR013319"
FT                   /db_xref="UniProtKB/TrEMBL:Q9V2T0"
FT                   /citation=[2]
FT                   /protein_id="AAD54602.1"
FT                   /translation="MSKKKFVIVSILTILLVQAIYFVEKYHTSEDKSTSNTSSTPPQTT
FT                   LSTTKVLKIRYPDDGEWPGAPIDKDGDGNPEFYIEINLWNILNATGFAEMTYNLTSGVL
FT                   HYVQQLDNIVLRDRSNWVHGYPEIFYGNKPWNANYATDGPIPLPSKVSNLTDFYLTISY
FT                   KLEPKNGLPINFAIESWLTREAWRTTGINSDEQEVMIWIYYDGLQPAGSKVKEIVVPII
FT                   VNGTPVNATFEVWKANIGWEYVAFRIKTPIKEGTVTIPYGAFISVAANISSLPNYTELY
FT                   LEDVEIGTEFGTPSTTSAHLEWWITNITLTPLDRPLIS"
FT   sig_peptide     82..138
FT                   /gene="eglA"
FT   mat_peptide     139..1038
FT                   /gene="eglA"
FT                   /product="endoglucanase A"
FT   misc_feature    163..228
FT                   /gene="eglA"
FT                   /note="Region: proline- and hydroxy-amino acid rich"
FT   variation       509
FT                   /gene="eglA"
FT                   /replace="c"
FT                   /note="corrected version of published sequence"
FT                   /citation=[2]
FT   misc_feature    670..672
FT                   /gene="eglA"
FT                   /note="catalytic nucleophile; active site"
FT                   /inference="non-experimental evidence, no additional
FT                   details recorded"
FT   misc_feature    919..921
FT                   /gene="eglA"
FT                   /note="incorrectly presented as encoding lysine instead of
FT                   leucine"
FT                   /citation=[2]
FT   misc_feature    949..951
FT                   /gene="eglA"
FT                   /note="catalytic nucleophile; active site"
FT                   /inference="non-experimental evidence, no additional
FT                   details recorded"
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SQ   Sequence 1134 BP; 392 A; 228 C; 223 G; 291 T; 0 other;
     gaatgtcggg gcagattacg aagagagaag agctcttttt tataccttat attcaaatct        60
     aacaaatttg gaggatgtcc aatgagcaag aaaaagttcg tcatcgtatc tatcttaaca       120
     atccttttag tacaggcaat atattttgta gaaaagtatc atacctctga ggacaagtca       180
     acttcaaata cctcatctac accaccccaa acaacacttt ccactaccaa ggttctcaag       240
     attagatacc ctgatgacgg tgagtggcca ggagctccta ttgataagga tggtgatggg       300
     aacccagaat tctacattga aataaaccta tggaacattc ttaatgctac tggatttgct       360
     gagatgacgt acaatttaac cagcggcgtc cttcactacg tccaacaact tgacaacatt       420
     gtcttgaggg atagaagtaa ttgggtgcat ggataccccg aaatattcta tggaaacaag       480
     ccatggaatg caaactacgc aactgatggc ccaataccat tacccagtaa agtttcaaac       540
     ctaacagact tctatctaac aatctcctat aaacttgagc ccaagaacgg actgccaatt       600
     aacttcgcaa tagaatcctg gttaacgaga gaagcttgga gaacaacagg aattaacagc       660
     gatgagcaag aagtaatgat atggatttac tatgacggat tacaaccggc tggctccaaa       720
     gttaaggaga ttgtagtccc aataatagtt aacggaacac cagtaaatgc tacatttgaa       780
     gtatggaagg caaacattgg ttgggagtat gttgcattta gaataaagac cccaatcaaa       840
     gagggaacag tgacaattcc atacggagca tttataagtg ttgcagccaa catttcaagc       900
     ttaccaaatt acacagaact ttacttagag gacgtggaga ttggaactga gtttggaacg       960
     ccaagcacta cctccgccca cctagagtgg tggatcacaa acataacact aactcctcta      1020
     gatagacctc ttatttccta aatttcggca acctgggaat tatcaagttt aagaaaaggt      1080
     ggagttgcta aagaattcaa agaaaatttg aaaagtaact tttattgtga tctc            1134
//