 |
|
EBI DbfetchID AF163837; SV 1; linear; genomic DNA; STD; PRO; 4567 BP. XX AC AF163837; XX DT 02-JAN-2000 (Rel. 62, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Caldibacillus cellulovorans multidomain beta-1,4-mannanase precursor (manA) DE gene, complete cds; and unknown genes. XX KW . XX OS Caldibacillus cellulovorans OC Bacteria; Firmicutes; Bacillales; Alicyclobacillaceae; Caldibacillus. XX RN [1] RP 1-4567 RX DOI; 10.1128/AEM.66.2.664-670.2000 RX PUBMED; 10653733. RA Sunna A., Gibbs M.D., Chin C.W.J., Nelson P.J., Bergquist P.L.; RT "A gene encoding a novel multidomain beta-1,4-mannanase from Caldibacillus RT cellulovorans and action of the recombinant enzyme on kraft pulp"; RL Appl. Environ. Microbiol. 66(2):664-670(2000). XX RN [2] RP 1-4567 RA Sunna A., Gibbs M.D., Bergquist P.L.; RT ; RL Submitted (29-JUN-1999) to the EMBL/GenBank/DDBJ databases. RL Biological Sciences, Macquarie University, North Ryde, New South Wales RL 2109, Australia XX FH Key Location/Qualifiers FH FT source 1..4567 FT /organism="Caldibacillus cellulovorans" FT /mol_type="genomic DNA" FT /db_xref="taxon:74586" FT CDS <1..513 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="orf1" FT /db_xref="GOA:Q9RFX6" FT /db_xref="HSSP:1NBC" FT /db_xref="InterPro:IPR001956" FT /db_xref="UniProtKB/TrEMBL:Q9RFX6" FT /protein_id="AAF22273.1" FT /translation="TPTPTPTPSAAPTPTPSAGGSLVVQYRAADTNAGDNQLKPHFRIV FT NRGTSSVPLSELTIRYWYTVDGDKPQVFNCDWAQVGCSNVRGSFVKLSTGRTGADYYIE FT ITFTSGAGSLAAGASSGDIQVRINKNDWTNYNEANDYSYDPTKTSFADWNRVTLYRNGQ FT LIWGVEP" FT misc_feature 1..60 FT /note="Region: proline-threonine linker" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 61..510 FT /note="Region: cellulose-binding domain type IIIb" FT /inference="non-experimental evidence, no additional FT details recorded" FT sig_peptide 620..718 FT /gene="manA" FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 620..3412 FT /codon_start=1 FT /transl_table=11 FT /gene="manA" FT /product="multidomain beta-1,4-mannanase precursor" FT /db_xref="GOA:Q9RFX5" FT /db_xref="HSSP:3MAN" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9RFX5" FT /protein_id="AAF22274.1" FT /translation="MNRRLIARLSGMLAMVLIAAMLAYVPKPEPAEAHGGMVFPATRTY FT ACYVDGKVHGNGGDLNMINPACLDALAISGNYQFWNWFGNLISNAGGRHREIIPDGKLC FT GPTASFDGMNQARTDWWTTRLQPGATITVRVNAWAPHPGTWYLYVTRDGWDPTQPLKWS FT DLEPTPFSQVTNPPINSSGPDGAEYSWQVQLPNKQGRHIIYMIWQRSDSPEAFYNCSDV FT YFGSGPIAYEFGDPREGGTMITPPPSGTTPTPTPTPTPTSTPTPTPTPSVTPTVTPTST FT PTPSASGTLRVEYRVGDTSATDNQMKPQLRIVNTGSQAVPLTELKVRYWYTKNSTQAEQ FT YFCDWAQIGCSNIRAQFVSLSQPVSGADSYIELSFTGGSIPAGGNTGEIQNRIHFTNWM FT NYNETDDWSYNGAQTTWGPSTRITLYRNGVLVWGTEPGGGSSPPTPTVTPTPTPTPTST FT PTPTPTPTSTPTPSGGPNLSVNTQGLVGINHPHAWYRDRLSSSLQGIRSWGANAVRIVL FT SNGCRWTKIPASEVADIISQARTLGYRAVVLEVHDTTGYGEDAAACSMTTAVNYWIELK FT NVLAGQENFVIVNIGNEPYGNNNYQNWVTDTRNAVQALRNAGINNTIMVDAPNWGQDWS FT FTMRDNAPTIFNADPQRNLVFSIHMYGVYDTAAEVQSYIESFVNRGLPLVIGEFGHMHS FT DGDPNEQAIVQYAKQYNIGLFGWSWSGNGGGVEYLDMVTNFNANSPTAWGTWFRTNAIG FT TSTSPTPTPTPTPTPTPTPTPTPSAGGNLVVQYRAADTNATDNQLKPHFRIVNRGTSSV FT PLSELTIRYWYTVDGDKPQVFNCDWAQVGCSNLRGSFVKLSTGRTGADYYIEITFTSGA FT GSLAPGASSGDIQVRINKNDWTNYNEANDYSYDPTKTSFADWNRVTLYRNGQLVWGVEP FT " FT mat_peptide 719..3409 FT /gene="manA" FT /product="multidomain beta-1,4-mannanase" FT misc_feature 1349..1480 FT /gene="manA" FT /note="Region: proline-threonine linker" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 1481..1927 FT /gene="manA" FT /note="Region: cellulose-binding domain type IIIb" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 1928..2032 FT /gene="manA" FT /note="Region: proline-threonine linker" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 2033..2884 FT /gene="manA" FT /note="Region: beta-1,4-mannanase catalytic domain" FT /experiment="experimental evidence, no additional details FT recorded" FT misc_feature 2885..2959 FT /gene="manA" FT /note="Region: proline-threonine linker" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 2960..3409 FT /gene="manA" FT /note="Region: cellulose-binding domain type IIIb" FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 3894..4400 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="orf3" FT /db_xref="UniProtKB/TrEMBL:Q9RFX4" FT /protein_id="AAF22275.1" FT /translation="MGASGVFSRTAQNGYGLKARCQKRRCVGRNLLGHRLFVVDDDEFR FT FFAGARKRLGRFSRRQNRLGEAQNFESGTVTFVQPDDKRRIRRENIGQLFVRFQASIGP FT KRLIEIAEQRQARARKQASDQPQLKNAVILHFIDQHMPYGVERMDPFHKFIEKQKSRSI FT LTRYY" XX SQ Sequence 4567 BP; 1040 A; 1276 C; 1439 G; 812 T; 0 other; acaccgacac ctacgcctac gccgagcgcg gcgcccacac cgacgccgtc ggccggcggc 60 agcctggtcg tgcagtatcg cgcggcggac acgaacgcgg gcgacaacca gctgaagccg 120 cattttcgga ttgtgaaccg cgggacgtcg agcgtgccgc tgtcggagct gacgattcgg 180 tactggtaca cggtggacgg ggacaagccg caggtgttca actgcgactg ggcgcaggtg 240 ggttgttcga acgtgcgcgg cagcttcgtg aagctttcga cgggccggac gggggcggac 300 tactatatcg agatcacgtt cacgtcgggc gcgggcagct tggcggctgg ggcgagcagc 360 ggggacattc aggtgcggat caacaagaac gactggacga actacaacga ggcgaacgac 420 tactcgtatg atccgacgaa gacgagtttt gcggattgga accgggtgac gctgtatcgc 480 aacggtcagc tcatctgggg cgtcgagcct tgattgccaa cgacagtatg gacctggcgg 540 acggtaagtc cgttcgccag gttcctataa aaacaacatc tccgctcgaa aaaaccatgg 600 aggaagggag aggattttta tgaatcgacg gcttatcgcc cgcctcagcg gcatgttggc 660 gatggttctc atcgccgcaa tgttggcgta cgttccgaag cctgaaccgg ccgaggcaca 720 cggaggtatg gtgtttccag ccacgcgaac gtatgcctgt tatgtcgacg gcaaggttca 780 cggcaatggc ggagacttga acatgatcaa ccctgcgtgt cttgacgcct tggcgatctc 840 gggcaactat caattctgga actggttcgg aaatctgatc agtaatgccg gaggacgcca 900 tagggaaatc attcctgacg gcaaactgtg cggaccaacg gccagttttg atggtatgaa 960 ccaggcgcgt acagactggt ggacgactcg tctgcagccg ggcgcaacga ttacggtgcg 1020 agtcaacgca tgggcgccgc atcccggcac gtggtatttg tatgtaaccc gggacggatg 1080 ggatccgacg caaccattga aatggtcgga tctggaaccg acgcccttca gccaggtgac 1140 taatccgccg atcaactcga gcggaccgga cggggccgag tacagctggc aggtacagct 1200 gccgaacaag caaggtcgac acatcattta tatgatatgg cagagatctg atagtccaga 1260 ggcattctac aactgttcgg atgtttattt cggatcgggg ccgattgctt atgaatttgg 1320 tgacccgcgg gaaggcggaa cgatgattac gccgccgccg tcgggcacga cgccgacgcc 1380 gacaccgacg ccgacaccca cgtcgacgcc gacaccgacg cccacaccga gtgtgacacc 1440 aaccgtgaca ccgacgtcga cgccgacgcc tagcgcaagc ggcaccctgc gcgtcgagta 1500 tcgcgtgggc gacacgagcg ccaccgacaa ccagatgaaa ccgcagctgc gcatcgtcaa 1560 caccggctcg caagccgtgc cgctgaccga gctgaaggtg cgctactggt acacgaagaa 1620 ctcgacgcag gccgaacagt acttctgcga ctgggcgcag atcggctgct cgaacatccg 1680 ggcgcagttc gtgtcgctgt cgcagccggt cagcggggcg gacagctaca tcgagctgag 1740 cttcacgggc ggaagcattc cggcgggagg caacacgggc gagattcaga accggattca 1800 cttcacgaac tggatgaact acaacgaaac ggacgactgg tcgtacaacg gggcgcagac 1860 gacgtggggg ccgtcgacgc ggattacgct ttatcgcaac ggggtgctgg tatgggggac 1920 ggagccgggc ggcggatcgt cgccgccgac gccgacggtg acaccgacac cgacaccgac 1980 gccgacgtca acgccgacac ctacgcctac gccaacatca acgccgacac cgagcggtgg 2040 accgaacctg agcgtcaata cccaaggtct ggttggtatt aatcacccgc atgcgtggta 2100 tcgcgaccgg ctcagttcgt cgttgcaagg cattcgttcc tggggcgcca acgcggtgcg 2160 gatcgtgctg agcaacggtt gccggtggac gaagattccg gccagcgaag tagcggacat 2220 tatttctcag gcgcggacgc tcggttatcg ggcggtcgtt cttgaagttc acgacacgac 2280 cggctacggg gaagacgcgg cggcgtgttc gatgacgacg gcggtcaatt attggattga 2340 actgaagaac gtgctggcgg gtcaggagaa tttcgtcatc gtcaatatcg gcaatgagcc 2400 gtacgggaac aataattatc agaactgggt gacggacacg cggaacgcgg tgcaggcgct 2460 gcggaacgcg gggatcaaca atacgatcat ggtggacgct ccgaactggg gtcaggactg 2520 gtcgttcacg atgcgggaca acgcgccgac gattttcaac gcggacccgc agaggaacct 2580 ggtcttttca atccacatgt acggggtgta cgacacggcg gcggaagtgc aaagttatat 2640 tgagtcgttc gtaaaccgcg ggttgccgct ggtgatcggg gaatttggtc atatgcacag 2700 cgacggggat ccgaacgaac aggcgatcgt acaatatgcg aagcaataca acattggctt 2760 gttcggctgg tcgtggagcg gtaacggagg cggcgtggaa tatttggaca tggtgacgaa 2820 cttcaatgcg aacagcccga cggcgtgggg gacatggttc cggacgaacg cgatcgggac 2880 ctcgacttcg ccgacaccga cacctacacc gacgccgacg ccaacgccga cgccgacacc 2940 gacgccgtcg gccggcggca acttggtcgt acagtaccgc gcggcggaca cgaacgcgac 3000 ggacaaccag ctgaagccgc attttcggat tgtgaaccgc gggacgtcga gcgtgccgct 3060 gtcggagctg acgattcggt actggtacac ggtggacggg gacaagccgc aggtgttcaa 3120 ctgcgactgg gcgcaggtgg gttgctcgaa cttgcggggc agcttcgtga agctttcgac 3180 gggccggacg ggggcggact actacatcga gatcacgttc acgtcgggcg cgggcagctt 3240 ggcgcctggg gcaagcagcg gggacattca ggtgcggatc aacaagaacg actggacgaa 3300 ctacaacgag gcgaacgatt actcgtatga tccgacgaag acgagttttg cggattggaa 3360 ccgggtgacg ctgtatcgga acggtcagct cgtctggggc gtcgagccgt aaccggagac 3420 cgccgacatt cgaagcaccg cgggcgccga cgaaagacga aacgagaaca aaccacagcc 3480 gaaatcaaaa acggcagctc ggccgagacg aaaacggacg gccgggctgc ctttttctct 3540 ttttggcttt cggaagttta tcggagccgc gggaacggtt tttcggaaca acgcctttcc 3600 acgttataga gcgaaagagg gatgtcgtcc ataaacggcg acggtcgccc gacggcatac 3660 aggatcaacc ggtgcagcgc ccgcgtacac atcgtgtaaa agagcttccg ctccgtttcc 3720 tgaaaaaaca cgtcggccga cgcattccag aggagcacga cgtcgaattc gagtcctttg 3780 gccaaataag acggcagaat cgtccaccct tttacgaacg cgacgtcgtc cttcgtcagg 3840 agcctcggcc gcaagtgcgt cgtttcggcg gcggcgatcg catccagcag gcgatgggcg 3900 cgtccggcgt cttcagccgt acggcacaaa acggctacgg actgaaagcc cgatgccaaa 3960 agagacgatg cgtcggccga aacttgttgg gccatcgctt gttcgtcgtc gacgatgacg 4020 agtttcggtt tttcgccgga gcgcgcaaac ggctcggacg gttctcccgc cggcagaatc 4080 gcttgggcga agcgcagaat ttcgagagtg gaacggtaac ttttgtgcaa ccggacgata 4140 aacggcgaat ccggcgcgaa aacatcggcc agttgttcgt acgattccag gccagtatcg 4200 ggccgaagcg tctgattgag atcgccgagc agcgtcaggc gcgcgcgcgg aaacaggcgt 4260 ctgatcagcc acagctgaaa aacgctgtaa tcttgcactt catcgatcaa cacatgccgt 4320 acggcgttga acggatggat cccttccaca aattcataga gaaacagaaa agcaggagca 4380 tcctcacaag gtattattga tttatccatt ttatctaaaa accaggatgc catttcgtcc 4440 caccgatccg gaacgcctgt gccggattcc gaggcgatcc gtgcaaacag cgcccgatct 4500 tcatgcaatc ggcggtacat acccgcaacg tccgcaaact ccaatttttc gacttgcccg 4560 cgaattt 4567 // |
|