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EBI DbfetchID AF160196; SV 2; linear; genomic DNA; STD; PLN; 6385 BP. XX AC AF160196; XX DT 22-JUL-1999 (Rel. 60, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 3) XX DE Vigna unguiculata glycinamide ribonucleotide transformylase (pur3) gene, DE complete cds. XX KW . XX OS Vigna unguiculata (cowpea) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; OC eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Vigna. XX RN [1] RP 1-6385 RA Hall D.J., Bussell J.D., Mann A.J., Goggin D.E., Atkins C.A., Smith P.M.C.; RT "Partial genomic sequence of VUpur3, the gene encoding glycinamide RT ribonucleotide transformylase"; RL Unpublished. XX RN [2] RP 1-6385 RA Smith P.M.C., Mann A.J., Hall D.J., Atkins C.A.; RT ; RL Submitted (17-JUN-1999) to the EMBL/GenBank/DDBJ databases. RL Botany, The University of Western Australia, Nedlands, WA 6907, Australia XX RN [3] RC Nucleotide and amino acid sequences updated by submitter RP 1-6385 RA Bussell J.D., Smith P.M.C.; RT ; RL Submitted (02-DEC-2002) to the EMBL/GenBank/DDBJ databases. RL Plant Biology, University of Western Australia, 35 Stirling Highway, RL Crawley, WA 6009, Australia XX CC On Dec 2, 2002 this sequence version replaced gi:5566265. XX FH Key Location/Qualifiers FH FT source 1..6385 FT /organism="Vigna unguiculata" FT /cultivar="Vita3" FT /mol_type="genomic DNA" FT /db_xref="taxon:3917" FT mRNA join(774..1011,1123..1666,1931..2042,2270..2446,2544..2670, FT 3364..3491,3593..3896) FT /gene="pur3" FT /product="glycinamide ribonucleotide transformylase" FT /note="class 1" FT exon 774..1011 FT /gene="pur3" FT /number=1 FT mRNA join(804..1011,1123..1666,1931..2042,2270..2446,2544..2670, FT 3364..3491,3593..3959) FT /gene="pur3" FT /product="glycinamide ribonucleotide transformylase" FT /note="class 1; alternative poly_A site" FT mRNA join(889..1011,1123..1666,1931..2042,2270..2446,2544..2670, FT 3364..3738) FT /gene="pur3" FT /product="glycinamide ribonucleotide transformylase" FT /note="class 2" FT mRNA join(893..1011,1123..1666,1931..2042,2270..2446,2544..2670, FT 3364..3491,3593..3660,6290..>6385) FT /gene="pur3" FT /product="glycinamide ribonucleotide transformylase" FT /note="class 3" FT exon 1123..1666 FT /gene="pur3" FT /number=2 FT CDS join(1258..1666,1931..2042,2270..2446,2544..2670, FT 3364..3477) FT /codon_start=1 FT /gene="pur3" FT /product="glycinamide ribonucleotide transformylase" FT /function="catalyzes the third step of the purine FT biosynthesis pathway" FT /EC_number="2.1.2.1" FT /note="phosphoribosyl glycinamide formyl transferase; GAR FT transformylase" FT /db_xref="GOA:P52423" FT /db_xref="InterPro:IPR004607" FT /db_xref="UniProtKB/Swiss-Prot:P52423" FT /protein_id="AAD45353.2" FT /translation="MEAQQIISRFCPKSSLAPSIPMVKQPFSLNFPPLHSLSSYPFLQS FT QNLGFPTGALHAISFVHKEVCSSSWRIWCSKSSSSTAEPEEDHEVRAQVTVRRKKLAVF FT VSGGGSNFRSIHEASKKGSLHGDVTVLVTNKSECGGAQYARNNGIPVILFPKAKDEPKG FT LSPCDLVDTLRKFEVDFVLLAGYLKLIPVELIRAFERSIFNIHPSLLPAFGGKGYYGMK FT VHKAVIASGARFSGPTIHFVDEHYDTGRILAQRVVPVLANDTAEELAARVLNEEHQLYV FT EVVEALCEERIVWRKDGVPLIQSRENPNEFL" FT misc_feature 1321..1323 FT /gene="pur3" FT /note="alternative translational start codon" FT exon 1931..2042 FT /gene="pur3" FT /number=3 FT exon 2270..2446 FT /gene="pur3" FT /number=4 FT exon 2544..2670 FT /gene="pur3" FT /number=5 FT exon 3364..3491 FT /gene="pur3" FT /number=6 FT exon 3593..3660 FT /gene="pur3" FT /number=7 FT polyA_signal 3708..3713 FT /gene="pur3" FT /note="putative" FT polyA_site 3738 FT /gene="pur3" FT polyA_signal 3862..3867 FT /gene="pur3" FT /note="putative" FT polyA_site 3896 FT /gene="pur3" FT polyA_signal 3930..3935 FT /gene="pur3" FT /note="putative" FT polyA_site 3959 FT /gene="pur3" FT exon 6290..>6385 FT /gene="pur3" FT /number=8 XX SQ Sequence 6385 BP; 2009 A; 1052 C; 1111 G; 2212 T; 1 other; gagaaggaca aacccaaaac cccaaaatct atttcaacgc aaaaagctta aaaccctaaa 60 attgatttcg aaaaaattgg aagctcacct agattgttga atgccttcga tcacaatgtc 120 gaaaattaat ctgatgcagt gccaatcaca atcacaatcg aacccaatcg aaccctagct 180 tagtgttctc cacgatcacc ttcgaacact tctcagctca taaccttgca cataaccatt 240 gatacgggcc acgtcactga aactcataca cagtcataaa gccatgtcac tgttccgtta 300 cgatttttaa cggtgttaat tttggggacc aaattgaatc acttttgaca attagaggac 360 taaattgaga ctccaaatat ttagaggact ttttgaaaca tcaaaacaaa ttcgaggact 420 aaaatagtaa ttaagcctac aatttaatat aaaagaaagt aagaattctt tagaatatta 480 ttattaattt gcttattatt atttaggatt atttttgttt aaaacaattt caatttaaaa 540 tacttcaaca taggacagga ttcagttgcc gccacacagt gtttcttcgc tctcaccaat 600 gaccaacttt gcgtcgtaac ctcgtccttg tcgaatcttt ctttcgctgt tcctttcttc 660 gtcactcgtt caataacgcg ttcacaagcg tcactcctct gcagacccaa aaggtatttt 720 gattgcccac agttgttgct gttgattttt cttggtgcat ttgatatttg gaactgtgct 780 tgtgatttta gattctgttt atattttttg ggtttgtcct tctcatattg cttttctaca 840 aatgttgcta tggattttgg gattaagctt ctgattttga tttcccacaa ttgaaaaatc 900 gtgcttttgt tatctgggtt ttctcgtctt ggtcatcttt tttatattcc gaagaacttg 960 tttgtgtaag caaccccatt ggagttgttc taaaaggtct tcttgtttta ggtctggttt 1020 acttgtgtgc ttttacacga taacttttgt tttttcttgg ctttcttatt gctaatagtt 1080 actaattgat gtgagtttct ttgtgatgca atggaagttt agagcgctgt cttgaagatc 1140 gtaattgtgt tttcatttgg ccccttcaat caactttacc tatcccttac cttaactttt 1200 caacctcgcc aacagcatca aatactataa tagacctctg tttacattaa tttttccatg 1260 gaagctcaac agattatttc cagattttgc ccaaaatcat cactagcgcc atcaattcca 1320 atggtgaagc aaccgttctc tttaaatttc cctcctctac actctttatc gtcataccct 1380 tttctccagt ctcagaatct tggcttccct actggagctt tacatgctat cagctttgtg 1440 cacaaagaag tttgttctag ttcttggaga atatggtgta gcaagagtag tagtagtact 1500 gcagaaccag aggaggacca tgaggtgagg gctcaggtca cagtgagaag gaaaaagcta 1560 gcagttttcg tgtctggggg aggatccaac tttagatcaa ttcacgaggc ctctaaaaag 1620 ggatcgcttc atggagatgt tactgtattg gtcacaaata aaagtggtaa ttctctatct 1680 taatatccct ttactttcat attttccctt ttgtgtcatc atttagctta gctaaattct 1740 tggttttcat agaagtgtat ctatctcctt caatcttagc ttataatagt cactagataa 1800 ctggattagg attccatgtc tgctaaactt ccttgtgttg cgcgggatgt ttgtttggac 1860 tattttttta tttccctttt attttgagcc ttggtctgct aaaatttgtt tttgtttctt 1920 gtaataccag agtgtggagg tgcacagtat gcaagaaata atggtatccc agttatattg 1980 ttccctaaag caaaggatga acccaaagga ttatctcctt gtgaccttgt tgatacacta 2040 aggtgtgatg cttctttaat tgatttcctg caattgtttt taactttaga cttcggggtc 2100 tttattcttt tcatgaatct gaggtgtaga ttctttgcaa agctgaaacg caaataaaaa 2160 aaccagtata actcaaatat gggaaaatcc atgaaaaggg acaaaggatg attttcactc 2220 tgcttcattg agtagttcca ttattaacat ataatacatt tttaatcagg aaattcgagg 2280 tggattttgt tcttttagct ggatacctca aactgatacc cgtagaattg atccgagctt 2340 ttgaaagatc tatattcaac attcatccat cacttcttcc agcttttgga ggcaagggct 2400 actatggcat gaaggttcat aaagcagtaa ttgcttctgg agcaaggtat aattctcatg 2460 aaatatctca agcacttcag tgaaacatta ttgtacttgg ttctttatac catgtctgcg 2520 cttcattttt ctgtgttggt caggttttca ggtccaacca ttcattttgt tgatgaacac 2580 tatgacaccg ggcgtatcct tgcacaacgt gtggtccctg tgcttgctaa tgatactgca 2640 gaggagttgg ctgcaagggt tctcaatgag gtaatgacag actatatatt ttaaactatt 2700 gaacattttt aatcctgaat ggagttcagc aaatatattt gtgataagca ttttaggaat 2760 gaatttaggg aacatctaaa atggaaaaaa ctctatagga ttatcataga aagaaaaata 2820 ggggaaaggg tcaaattgta aatgatagtt gggtcaagtt gttagttatt tccgttaagt 2880 ctgttggaag tcttttatac ataggacttt ttcccttgtc ggggggaatt tttttctaag 2940 tgtttttgtc ttactagttt tccattgtaa aatgaaagat gtttcttttg ggcgtctact 3000 ttgtatacct tggttcttca aagagtcttg tagatagtca atgcaaaagt tatacacttc 3060 tttttctctc ttgttgtgtc tttgtattgt tctttgttag gaattctgtt atgttctttt 3120 ctttttgctt caagagatta acacattcat gagatgatgc acttgttggg tcatcattta 3180 gttgttggtc tctctttggt tatacaactg ccttcctctc atctatgaaa ggaatgagaa 3240 atatcgatgt ttatctgtat ctttattaaa tgctagattt ttagagtagt ttagttattt 3300 gcttcctatc agtcaagttg gactcaattg attactgttt gctaattttt gttttaaatt 3360 taggagcacc aattatatgt tgaggtggtg gaggctttat gtgaagagcg tatcgtttgg 3420 aggaaagatg gtgttcctct catccaaagc agagaaaacc ccaatgagtt tctctgatta 3480 tcattaatat ggtatgatac tcttttactt tgagtactgt aaaaagtttt tgtatacatc 3540 tttttacaat gatgaagtct tgcatctgat gggacttttg tttctgttat aggggatttt 3600 ggttgtgaaa acttcacatt taccccaaag ctgttgtagt taatgaattg taactattag 3660 gtaggtaatt tctggtgcac tgatatcata tctctaaggc aacatataat aagcaagaaa 3720 tcacaaactt atcttctgat ctgatctgca taaactgttg tatgctcttg gtagatttta 3780 atatcaggtc atggagcata tttttggagt cctttttttc cctgcacggg tttccatgaa 3840 catgtcatga ttttgcttct taataaagaa attatctact atactatttg gatgagaaat 3900 ttgatgtgcc ataagacatt tttgtaattt ataaatagtt agtctgtgat tgaactgttt 3960 ctaaattgca aagcttttac tgctatgcat cacttctttg cttacccatt tatttacaag 4020 tattctcctg ctacctcaat tgttgagcat atttgatttg aagactgttg aaggtggtaa 4080 cccaaattaa aatttgatta atattactgt ggaaggttgt cataaaatat tatttaaaaa 4140 ataaaaaggt ataattactg atttggttcc taattttttg gtttaactca atttggtttc 4200 cttattatta gttggttcaa tttaatatcc aattattaaa tgttaaattg aaccaatcaa 4260 taataaaggg atcaaattaa actaaaccaa aaaattaaga aaccaatgaa taattatacc 4320 aaataaaaat gctaaatata agtagttcta ttgaaacgag ttcgcaatag cgaaggtaaa 4380 cattaaccga tctaaattgt aactgaggat taggaaatgt taaaagaata ccggtagagg 4440 ctttggaact tagaaaagtt ggtttgtcaa gggggattta gatataaagg aacctttttg 4500 tttttctttt gtaggtggat gttgaatatt gatattgcca aaaaaaaagg atttatataa 4560 ttgattgtag gaaaatcaca tttgctgctt gaagaaaatc catggtctat tgagaaaact 4620 aaattactga acatttctgg gttgaaaatg caacaaataa gttctaattt tttctgtatt 4680 attctatcaa aaaaatagta atttattatt acatttacat ttactacaaa agtgttacga 4740 tctgtggagg ttaaaaatgc aatttttgag tgctaaacct ctacaaatgt caattctgag 4800 gttagaaacc tgggaaataa gttctagcgc ttttcataca gtcactggta tttttctgac 4860 gatcataagc tctaatttta gttgtgtttt catattatgt ttgaaatgtt attaacaatt 4920 taaaactttg gaaatgatac aaattttaga gttatgacgg taagaattca atatagaaaa 4980 aagaaaatgc catagagtgc cacttgacag cggaagcaac ttcttcatgc tcgttcaaca 5040 gggatgcagt acaaaggtaa accaccacta gtgtagttaa taccaaactt agacacaaga 5100 gtaaaacaaa attcaacatc acattccaat gataatagaa acacaaaaga aataacaaaa 5160 ctcaaagcat atagcatcaa agggttgaaa taagatccat gactgaacaa aaataaacca 5220 ccaaatatat actaataaca ttaaatttac acataaacta aaaccctcat aaaaattcaa 5280 gaaacagtta cagaaatcca aatttgcaac ttaaacaaac aaagagctga aaataaaaac 5340 cactacattt agaaaatttg ttagctatcc tctaatagag aaacatactc aaagatcgaa 5400 gaaactcatt gtgaaaattt tcaatatatt tcaaacattg atccaaaaat acaaaccatg 5460 gttagaaaaa gtttgttaga gccantcaat agcaaaaggg gtacataatt gtaattgttt 5520 tatttggttt caaggtgttg gaggtgttca aggattattg gaagcttgtg tcaaagtttg 5580 tcaaaatggt tctttttgaa aagaaagagc atgtcacttt gttcacacta gcctcggtta 5640 gttatgcatt gtgacaaggc cacaatcttg tgattgtggt aactcggtgg tgcttaagag 5700 agtttctact tctaattgag aaatccaaac acaatcccat aggagttctt gtctttccca 5760 ccattccaat cacaacaatt caaacctcca aaacaaaacc ctaacaaaag ctcgatcact 5820 ctctcaagac aacatcatct cctaaatcaa cctaacacca acaattacac atacaaaaac 5880 aaattaaact tagacctaat agccttataa caatggtaca tggatactat cactacaaaa 5940 tttatcacaa ttatgtattt atcgatgctt gtcacaaata ctacaattac ataacaaaag 6000 ctgtcatgat ttgtgaagaa ccgattcttg ttcaaaattg tcattacaaa aggaaagttt 6060 tttttagtat tattataaag tgatgtagcc acaaaaacat tctcatgaca tgtatattaa 6120 atgtaggtcc tattagatat tacatataaa agtttgatat gaaacaatgg aaaaatttat 6180 tttccaagaa aatgaaggaa aagtaaaact catggaaaga gatgcggcat gttgatgttg 6240 aaatggggaa gcccacaaaa ttgaagaggg tctgtgttgt ttgttgaagg tgttgtctgc 6300 attgcaaggt gatcaaaagc tgggttgaag aagctcatgt tcatcaaatt atttgcagaa 6360 ggaggcatat tgttgcactg tgtag 6385 // |
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