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EBI DbfetchID AF158023; SV 1; linear; genomic DNA; STD; PRO; 4097 BP. XX AC AF158023; XX DT 20-SEP-1999 (Rel. 61, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Rhizobium meliloti sulfate activation locus, complete sequence. XX KW . XX OS Sinorhizobium meliloti OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; OC Sinorhizobium/Ensifer group; Sinorhizobium. XX RN [1] RP 1-4097 RX PUBMED; 10464198. RA Abola A.P., Willits M.G., Wang R.C., Long S.R.; RT "Reduction of adenosine-5'-phosphosulfate instead of RT 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium RT meliloti and other members of the family Rhizobiaceae"; RL J. Bacteriol. 181(17):5280-5287(1999). XX RN [2] RP 1-4097 RA Willits M.G., Abola A.P., Long S.R.; RT ; RL Submitted (10-JUN-1999) to the EMBL/GenBank/DDBJ databases. RL Howard Hughes Medical Institute and Dept. of Biological Sciences, Stanford RL University, Stanford, CA 94305-5020, USA XX FH Key Location/Qualifiers FH FT source 1..4097 FT /organism="Sinorhizobium meliloti" FT /strain="Rm1021" FT /mol_type="genomic DNA" FT /db_xref="taxon:382" FT CDS 419..1216 FT /codon_start=1 FT /transl_table=11 FT /gene="cysH" FT /product="adenosine-5'-phosphosulfate reductase" FT /function="catalyzes the reduction of FT adenosine-5'-phosphosulfate (APS) to free sulfite and ATP FT with thioredoxin cofactor" FT /note="APS reductase" FT /db_xref="GOA:P56891" FT /db_xref="InterPro:IPR002500" FT /db_xref="InterPro:IPR011798" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:P56891" FT /protein_id="AAD55759.1" FT /translation="MTTQSLKAEAVALEADVMALDAEAKALNDKLESLDLAGRLALIAG FT LEGRAVFTTSLGIEDQVITAAIGSNRLDIEVATLKTGRLFNETVALIDQTEETYDILIK FT RYYPEKADIDAYVAQYGMNGFYESVEARHACCGVRKLKPLARALDGASYWITGLRRGQS FT GNRATTPFAEADVERGLIKINPLADWGIETIQAHVAAEGIPVNPLHSRGYPSIGCEPCT FT RAIKPGEPERAGRWWWENDEKRECGLHVPEAASSIIPNASNAA" FT CDS 1264..2217 FT /codon_start=1 FT /transl_table=11 FT /gene="cysD" FT /product="ATP sulfurylase subunit CysD" FT /function="catalyzes the addition of free sulfate to ATP to FT make adenosine-5'-phosphosulfate (APS) and PPi together FT with the product of the cysN gene" FT /EC_number="2.7.7.4" FT /note="requires GTP cofactor" FT /db_xref="GOA:P56892" FT /db_xref="InterPro:IPR002500" FT /db_xref="InterPro:IPR011784" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:P56892" FT /protein_id="AAD55760.1" FT /translation="MPHTLPETELHNPQSTKPPLDPHLKALENEAIHIFREVAAEFERP FT VMLYSIGKDSSVLLHLARKAFYPGRIPFPLLHVDTGWKFREMIAFRDEMVAKYDLDLVA FT HTNPRGAAENVTPFTHGSALYTDIMKTEALRQALDAGQYDAAFGGARRDEEASRAKERI FT YSFRTPDHRWDPRNQRPELWNVYNGMIRKGESVRAFPLSNWTEVDIWRYIQAEDIPIVP FT LYFAKKRPVVERDGMLILAEDPRLELLPGEVKREEVIAFRTLGCFPLTGAIRSEADTLD FT DIIAELETATVSERQGRAIDRDQAGSMEKKKREGYF" FT CDS 2217..3713 FT /codon_start=1 FT /transl_table=11 FT /gene="cysN" FT /product="ATP sulfurylase subunit CysN" FT /function="catalyzes the addition of free sulfate to ATP to FT make adenosine-5'-phosphosulfate (APS) and PPi together FT with the product of the cysN gene" FT /EC_number="2.7.7.4" FT /note="requires GTP cofactor" FT /db_xref="GOA:P56893" FT /db_xref="InterPro:IPR000795" FT /db_xref="InterPro:IPR004161" FT /db_xref="InterPro:IPR005225" FT /db_xref="InterPro:IPR009000" FT /db_xref="InterPro:IPR009001" FT /db_xref="InterPro:IPR011779" FT /db_xref="UniProtKB/Swiss-Prot:P56893" FT /protein_id="AAD55761.1" FT /translation="MTAPAAANAALDQTVVSLPVQETARVVRDTRPLRLITCGSVDDGK FT STLIGRLLWDTKAVKEDQAASLQRDSSGKQNDLGLPDFALLLDGLQAEREQGITIDVAY FT RYFATDKRSFIVADTPGHEQYTRNMATGASTADLAVLLVDARVGLLEQTRRHATIATLM FT GIRQFVLAVNKIDLTNYDRARFDQISHEFRELALSLGVRQVTAIPVSALKGENVVYDGR FT ASMPWYDGPTLIEILELATTRSAQTVGFRLPVQRVSRPGESFRGYQGTVAGGSVKPGDS FT VVILPSGMVANVSKIVTFDLVRNAAVAGDAITLVLDRQVDVSRGDVIASIDSQPTTGLA FT FDAQLVALQPEGIQPGKRYWLKSGSRRQRVQVQPVSQLELKSGKWNHADELPMNAIGKV FT HLAFDEQAIFDTYEQNRTTGAFILIDPDTNNTVAGGMITAKRAALGGIHAEESRVILSL FT PADLADQLMATELFASRREDVEVRRVTAGKAVNIIDAIDG" XX SQ Sequence 4097 BP; 812 A; 1349 C; 1231 G; 705 T; 0 other; agatctcgcg tatcgtccct gaaagctcga cctgatcggc ctccgcccag gaccagccgt 60 gatgcgcata ggcgcccgtc accagaagca ggccgaaagc cactccagcg aatatgcggg 120 acggcaagga ttttgccatg tctttgtctc ctttcgtggg ctcgcggggt cagtctgaaa 180 gtggagcacg cgaagcagat ggacagagca ttcaacgatt gttgaacggc agcaccgcgc 240 gcgaagacag ctggcgggtt taatcgtaag acaataatct actggattac taggttttct 300 tagaacttta tggatcagag agatatttgc tctgatcctt gagcaataga aatccgtttt 360 tctgtccatt gacaccagcg gctgccatat tccgtgcaaa ttgcaacagg atgcagccat 420 gacgacgcaa tccctcaaag cagaagcggt ggccctcgaa gcagacgtga tggccctcga 480 tgcggaggcg aaggccctca acgacaagct cgaaagcctg gatctcgccg gccggctggc 540 gctcattgcc gggcttgaag gccgcgcggt ctttaccacg tcgctcggca tcgaggacca 600 ggtcattacc gccgcgatcg gcagcaaccg tctcgacatc gaagtcgcga cgctgaagac 660 gggccgcctc ttcaacgaga cggtggcgct gatcgaccag accgaggaaa cctatgacat 720 cctcatcaag cgctattatc ctgagaaagc cgacatcgac gcctatgtgg cgcaatacgg 780 catgaacggg ttctacgaaa gcgtggaagc ccggcatgcc tgttgcggcg tgcgcaagct 840 gaagccgctt gcgcgcgcgc tcgacggagc aagctattgg atcacgggcc tgcgccgcgg 900 gcagtcgggc aaccgtgcca cgaccccttt cgctgaagcc gacgtcgagc gcggactcat 960 caagatcaat ccgctcgccg actggggcat cgagacgatc caggcccatg tcgcggcaga 1020 gggcattccc gtcaacccgc tgcacagtcg cggctatccg tcgatcggtt gcgagccctg 1080 cacccgtgcc atcaagcccg gcgagccgga gcgcgccggc cgctggtggt gggagaacga 1140 cgagaagcgc gaatgcggcc tgcatgtgcc ggaagcggct tcctcgatca tccccaacgc 1200 cagcaacgcg gcctgagcgc cacgcgccgc acccgaagac cacgatcttc cccggagtca 1260 gcaatgcccc atactcttcc ggaaacggaa ctgcacaatc cgcagagcac gaaaccgccg 1320 ctcgatccgc atctgaaggc gctcgagaac gaagcgattc acatcttccg cgaggtcgcg 1380 gccgaattcg agcgcccggt gatgctctac tcgatcggca aggattcgtc ggtcctgctg 1440 catcttgccc gcaaggcctt ctatcccggc cgcatcccct tcccgctcct gcatgtcgac 1500 accggctgga agttccgcga gatgatcgca ttccgtgacg agatggtcgc gaaatacgat 1560 ctcgacctcg ttgcccacac caatccgcgc ggcgcagccg agaacgtcac gccgttcacg 1620 cacggttcgg cgctctatac cgacatcatg aagaccgaag cgttgcgcca ggcgctcgac 1680 gctggccagt acgacgccgc atttggcggc gcgcgccgtg acgaggaggc aagccgtgcc 1740 aaggagcgca tctattcctt ccgcacgccg gatcatcgct gggatccacg caaccagcgc 1800 ccggaactgt ggaacgtcta taacggcatg atccgcaagg gcgagagcgt ccgcgccttc 1860 cctctgtcca actggaccga ggttgacatc tggcgctaca tccaggcgga agatatcccg 1920 atcgtgccgc tctatttcgc caagaagcgc cccgtcgtag agcgcgacgg catgctgatc 1980 ctcgccgagg atcctcgtct cgagctgctt cccggcgagg tcaagcgcga ggaagtcatc 2040 gcgttccgca cgctcggctg cttcccgctc acgggtgcga tccgctccga agccgacacg 2100 ctcgacgaca tcattgccga acttgaaacc gcgaccgtct ccgaacgtca gggccgcgcg 2160 atcgaccgcg accaggccgg ttcgatggaa aagaagaaac gcgaaggata tttctgatga 2220 ccgcaccggc agcagcgaac gcagccctgg accaaaccgt cgtatccctc ccggtgcagg 2280 agactgcgcg ggtcgtgcgc gacacgcgcc cccttcgcct catcacctgc ggcagcgtcg 2340 atgacggcaa atcgacgctg atcggccggc tgctttggga caccaaggcg gtcaaggaag 2400 accaggcggc aagccttcag cgcgattcca gcggcaagca gaacgatctc ggcctcccgg 2460 atttcgctct cctgctcgac ggccttcagg ccgagcggga acagggcatc accatcgatg 2520 tcgcctatcg ctatttcgcg accgacaagc gctccttcat cgtcgccgac acgcccggcc 2580 acgagcaata cacgcgcaat atggcgaccg gcgcttcgac cgccgacctc gccgtgctgc 2640 tcgtcgacgc acgtgtcggc cttttggagc agacccgccg ccacgcgacg atcgcgacgc 2700 tgatgggcat ccgccagttc gtgctcgccg tcaacaagat cgacctgacg aactacgacc 2760 gcgcccgctt cgatcagatc tcgcacgagt tccgggaact ggccctttcg ctcggagtcc 2820 gccaggtcac cgcaattccg gtctcggcgc tcaagggcga gaacgtcgtc tatgacggcc 2880 gcgcttccat gccctggtat gacggcccga cgctgatcga gattctggag cttgccacga 2940 cccgctccgc ccagaccgtc ggcttccgcc tgccggtgca gcgcgtatcg cgcccgggag 3000 aaagcttccg cggttatcag ggcacggtcg ccggcggctc ggtgaagccg ggggactccg 3060 tcgtcatcct gccgtccggc atggtcgcca atgtgagcaa gatcgtcacc ttcgatctcg 3120 tgcgcaatgc ggcggttgcc ggcgacgcga tcacgctggt gctcgaccgc caggtggacg 3180 tttcccgcgg cgacgtgatc gcctcgatcg acagccagcc gacgaccggg ctcgccttcg 3240 atgcgcaact cgtggcgctg cagccggaag gcatccagcc gggcaagcgc tactggctga 3300 agtccggcag ccgccgccag cgcgtgcagg tgcagccggt cagtcagctc gaactcaaga 3360 gcggcaagtg gaaccacgca gacgaattgc cgatgaatgc gatcggcaag gtgcacctcg 3420 ccttcgacga acaggcgatc ttcgacacct atgagcagaa ccgcacgacc ggcgccttca 3480 tcctgatcga ccccgacacc aacaacacgg tggccggcgg catgatcacc gccaagcgcg 3540 ccgcgctcgg gggcatccat gccgaggaga gccgtgtgat cctgtctctg ccggccgatc 3600 tcgccgatca gctcatggca acggagctct ttgcaagccg ccgcgaagac gtggaggtgc 3660 gtcgtgtcac ggccggcaag gcggtcaaca tcatagacgc gattgacggg tgacctgaca 3720 gagtcatcga tgagcagagg ggccgtcagg cccctttttg ctgcgcagct ttcgacaggt 3780 tggcgattgg cgggttatca tggacgatca aggtgttgtc ctcgacatca tcaagatcgg 3840 ctctcgcggc agcgtctacg aataggagtc cgttatgcag accattacca ccccgggtgg 3900 cgaaaccctg gtcgtccttc ccattgctga atatgaagat ttgctcgata gggccgatat 3960 cgcgcaggcc gacaaggtga aggccgatat cgccgctggc cgcgacgagc tcgtgccggc 4020 cgaggtcgtc aaccgtcttc tggctggcga gaacgcggtc aaggtctggc gctcccatcg 4080 cggcatggcc gctcgag 4097 // |
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