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EBI DbfetchID AF156269; SV 1; linear; genomic DNA; STD; FUN; 2603 BP. XX AC AF156269; XX DT 02-NOV-1999 (Rel. 61, Created) DT 31-AUG-2006 (Rel. 89, Last updated, Version 3) XX DE Aspergillus niger 1,4-beta-D-glucan cellobiohydrolase B precursor (cbhB) DE gene, complete cds. XX KW . XX OS Aspergillus niger OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; OC Aspergillus. XX RN [1] RP 1-2603 RX PUBMED; 10508057. RA Gielkens M.M., Dekkers E., Visser J., de Graaff L.H.; RT "Two cellobiohydrolase-encoding genes from Aspergillus niger require RT D-xylose and the xylanolytic transcriptional activator XlnR for their RT expression"; RL Appl. Environ. Microbiol. 65(10):4340-4345(1999). XX RN [2] RP 1-2603 RA Gielkens M.M.C., Visser J., de Graaff L.H.; RT ; RL Submitted (03-JUN-1999) to the EMBL/GenBank/DDBJ databases. RL Section Molecular Genetics of Industrial Micro-organisms, Wageningen RL Agricultural University, Dreijenlaan 2, Wageningen 6708 CG, Netherlands XX DR CABRI; CBS 120.49. XX FH Key Location/Qualifiers FH FT source 1..2603 FT /organism="Aspergillus niger" FT /chromosome="II" FT /strain="CBS 120.49" FT /mol_type="genomic DNA" FT /clone="pIM3013" FT /db_xref="taxon:5061" FT TATA_signal 512..517 FT /gene="cbhB" FT mRNA 581..2341 FT /gene="cbhB" FT /product="1,4-beta-D-glucan cellobiohydrolase B precursor" FT CDS 608..2218 FT /codon_start=1 FT /gene="cbhB" FT /product="1,4-beta-D-glucan cellobiohydrolase B precursor" FT /EC_number="3.2.1.91" FT /db_xref="GOA:Q9UVS8" FT /db_xref="HSSP:1GPI" FT /db_xref="InterPro:IPR001722" FT /db_xref="UniProtKB/TrEMBL:Q9UVS8" FT /protein_id="AAF04492.1" FT /translation="MSSFQIYRAALLLSILATANAQQVGTYTTETHPSLTWQTCTSDGS FT CTTNDGEVVIDANWRWVHSTSSATNCYTGNEWDTSICTDDVTCAANCALDGATYEATYG FT VTTSGSELRLNFVTQGSSKNIGSRLYLMSDDSNYELFKLLGQEFTFDVDVSNLPCGLNG FT ALYFVAMDADGGTSEYSGNKAGAKYGTGYCDSQCPRDLKFINGEANCDGWEPSSNNVNT FT GVGDHGSCCAEMDVWEANSISNAFTAHPCDSVSQTMCDGDSCGGTYSASGDRYSGTCDP FT DGCDYNPYRLGNTDFYGPGLTVDTNSPFTVVTQFITDDGTSSGTLTEIKRLYVQNGEVI FT ANGASTYSSVNGSSITSAFCESEKTLFGDENVFDKHGGLEGMGEAMAKGMVLVLSLWDD FT YAADMLWLDSDYPVNSSASTPGVARGTCSTDSGVPATVEAESPNAYVTYSNIKFGPIGS FT TYSSGSSSGSGSSSSSSSTTTKATSTTLKTTSTTSSGSSSTSAAQAYGQCGGQGWTGPT FT TCVSGYTCTYENAYYSQCL" FT sig_peptide 608..670 FT /gene="cbhB" FT mat_peptide 671..2215 FT /gene="cbhB" FT /product="1,4-beta-D-glucan cellobiohydrolase B" FT misc_feature 671..1981 FT /gene="cbhB" FT /note="Region: catalytic domain" FT misc_feature 1982..2107 FT /gene="cbhB" FT /note="Region: linker peptide" FT misc_feature 2108..2215 FT /gene="cbhB" FT /note="Region: cellulose binding domain" XX SQ Sequence 2603 BP; 583 A; 773 C; 685 G; 562 T; 0 other; ggtaccgtaa tgggtagtag gtgatgttga atcaagcagt ggttgctctt tgggaatgac 60 ttcagttcct gacaactagc agaattgaat ttcggccgtt gggcgccccg ccgcgtagca 120 acgaggatct ttacccggtt tcggccatga tttgatgact caggcggggt gcggtgattg 180 ataagattag ggctagcagg tagggctacg ctgcgagtca catggtctgc actggatttc 240 ctctttgggc tcgtgagatg gctgtcccgc gcggattgcc ggatgcacaa cggcattgtc 300 tcctgcttat tgtggcggcg ccaccaggct gccggattac ctctttggta aagggaatgg 360 tcttccttga gttcgcttgt cacgatgact tcaagattgg acgccgacga gctcagtcta 420 ctatacgtgc catgtcggga tgcggtccgt ttagccgtaa catatccggg atcatgaagt 480 tgagagatag gcagcataca actgcaaaag gtataaagag ctcgttcttc tcctctgtcc 540 attatcacag cattcacatt cttcacagca gaactcagag gctcaagagc tcaagcagtg 600 aatcacaatg tcttccttcc aaatctaccg agcagctctg ctgctctcta tccttgccac 660 tgccaatgct cagcaggttg gcacctacac cactgagaca catccgtctc tgacctggca 720 aacctgcacc agcgatggca gctgcactac caacgacggc gaggtggtca tcgacgccaa 780 ttggcgttgg gtgcactcaa cctccagtgc caccaactgc tatactggca acgaatggga 840 cacctcaatt tgtaccgacg atgtgacctg cgccgcgaac tgcgcgcttg acggtgccac 900 ttacgaggcg acctatggtg tgaccacgtc cggcagcgag ctgcgcctga acttcgtcac 960 tcaaggctcc agcaagaaca tcgggtctcg tttgtacctc atgagcgacg acagcaacta 1020 tgagctgttc aagctcctcg gccaggagtt cacctttgat gtcgacgtgt ccaaccttcc 1080 ctgcggtctc aacggcgcgc tctacttcgt cgccatggac gctgacggtg gcacctcgga 1140 gtattccggc aacaaggccg gtgccaaata cggaaccggc tactgcgact cgcagtgccc 1200 tcgcgacctg aagttcatca acggcgaagc caactgcgac ggctgggagc cgtccagcaa 1260 caacgtcaac accggagttg gcgaccacgg ctcctgctgc gccgagatgg acgtctggga 1320 agcgaacagc atctccaacg ccttcaccgc gcacccctgc gactccgtca gccagacaat 1380 gtgcgacggt gactcctgtg gtggaactta cagcgccagc ggcgaccgct acagcggcac 1440 ctgcgacccc gacggctgcg actacaaccc ctaccgtcta ggcaacacgg acttttacgg 1500 ccccggcctg accgtcgaca cgaacagccc cttcaccgtc gtcacccagt tcatcaccga 1560 tgacggcacc tcctccggca ccttgaccga gatcaagcgg ttgtacgtgc agaacggcga 1620 ggtcatcgcc aacggcgcct ccacctactc cagtgtcaac ggcagctcta tcacctccgc 1680 tttctgtgaa tcagagaaga cgctgttcgg cgacgagaac gtcttcgaca agcacggcgg 1740 tctcgagggc atgggcgagg ctatggccaa gggcatggtc ttggtcttga gtctttggga 1800 tgactatgcc gctgacatgc tctggctcga cagcgactac cccgttaact cgtcggcctc 1860 gacccctggt gtggcccgcg gtacttgtag cacggactcg ggtgtcccgg ctaccgtgga 1920 ggcggagtca cccaatgcct atgtcacgta ctcgaacatc aagttcgggc ctattggctc 1980 gacttactcc agtggatctt cttcggggtc ggggtctagc tccagctcga gttcgactac 2040 cactaaggcc acttcgacga ccttgaagac tacctcgacc accagcagtg gaagcagttc 2100 gacatcggcg gcgcaggcgt atggacagtg tggtggacag ggctggactg gtccgaccac 2160 ttgtgtgagt gggtacactt gcacgtatga gaatgcgtac tactcgcagt gtttgtagac 2220 actactcgaa gggggatgaa gatggcagtg tggggggggg aatagttagt gattagtagt 2280 tagcacacgt actacagttt agtcaaattt aggaagcaat atgacatgat cacttgatgc 2340 agaatctacc aaccaccact ctacctcaaa ttacactcca ctataatcct actataaatt 2400 aacttcccgt acccgaccaa cctaggcaca aaccgaaccc aatcaaccat cacccaataa 2460 tcctccccct ctccttactt atctgcagaa catccgtgcg gaaagcctaa agtagcgctt 2520 acatgaccag acagtgtcac cccagacaca ccacatggct ggacgggata ttctccgcac 2580 ccaatgcatg catcctctgc agg 2603 // |
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