EBI Dbfetch

ID   AF152348; SV 1; linear; genomic DNA; STD; INV; 1290 BP.
XX
AC   AF152348;
XX
DT   02-JUN-2000 (Rel. 63, Created)
DT   06-JUL-2000 (Rel. 64, Last updated, Version 2)
XX
DE   Trypanosoma brucei brucei enolase gene, complete cds.
XX
KW   .
XX
OS   Trypanosoma brucei brucei
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
XX
RN   [1]
RP   1-1290
RX   PUBMED; 10889212.
RA   Haennert V., Brinkmann H., Nowitzki U., Lee J.A., Albert M.-A.,
RA   Sensen C.W., Gaasterland T., Muller M., Michels P., Martin W.;
RT   "Enolase from Trypanosoma brucei, from the amitochondriate protist
RT   Mastigamoeba balamuthi, and from the chloroplast and cytosol of Euglena
RT   gracilis: pieces in the evolutionary puzzle of the eukaryotic glycolytic
RT   pathway.";
RL   Mol. Biol. Evol. 17(7):989-1000(2000).
XX
RN   [2]
RP   1-1290
RA   Hannaert V., Albert M.-A., Brinkmann H., Nowitzki U., Michels P.A.M.,
RA   Martin W.;
RT   "Compartment-specific forms of the glycolytic enzyme enolase from
RT   Trypanosoma brucei and Euglena gracilis and a surprising evolutionary
RT   history of the genes";
RL   Unpublished.
XX
RN   [3]
RP   1-1290
RA   Hannaert V., Albert M.-A., Brinkmann H., Nowitzki U., Michels P.A.M.,
RA   Martin W.;
RT   ;
RL   Submitted (18-MAY-1999) to the EMBL/GenBank/DDBJ databases.
RL   ICP-TROP-74.39, Christian de Duve Institute of Cellular Pathology, Avenue
RL   Hippocrate 74 +1, Brussels B-1200, Belgium
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1290
FT                   /organism="Trypanosoma brucei brucei"
FT                   /sub_species="brucei"
FT                   /strain="427"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:5702"
FT   CDS             1..1290
FT                   /codon_start=1
FT                   /product="enolase"
FT                   /EC_number="4.2.1.11"
FT                   /note="2-phospho-D-glycerate hydrolase"
FT                   /db_xref="GOA:Q9NDH8"
FT                   /db_xref="InterPro:IPR000941"
FT                   /db_xref="PDB:1OEP"
FT                   /db_xref="UniProtKB/TrEMBL:Q9NDH8"
FT                   /protein_id="AAF73201.1"
FT                   /translation="MTIQKVHGREVLDSRGNPTVEVEVTTERGVFRSAVPSGASTGVYE
FT                   ACELRDGDKKRYVGKGCLQAVKNVNEVIGPALIGRDELKQEELDTLMLRLDGTPNKGKL
FT                   GANAILGCSMAISKAAAAAKGVPLYRYLASLAGTKELRLPVPCFNVINGGKHAGNALPF
FT                   QEFMIAPVKATSFSEALRMGSEVYHSLRGIIKKKYGQDAVNVGDEGGFAPPIKDINEPL
FT                   PILMEAIEEAGHRGKFAICMDCAASETYDEKKQQYNLTFKSPEPTWVTAEQLRETYCKW
FT                   AHDYPIVSIEDPYDQDDFAGFAGITEALKGKTQIVGDDLTVTNTERIKMAIEKKACNSL
FT                   LLKINQIGTISEAIASSKLCMENGWSVMVSHRSGETEDTYIADLVVALGSGQIKTGAPC
FT                   RGERTAKLNQLLRIEEELGAHAKFGFPGWS"
XX
SQ   Sequence 1290 BP; 318 A; 340 C; 361 G; 271 T; 0 other;
     atgacgatcc agaaagttca cggtcgcgaa gtccttgact ctcgtggtaa ccccacagtg        60
     gaggttgaag tgacgacaga gaggggtgtg ttccggtcag ctgtgccgtc tggtgcatcg       120
     accggtgtgt atgaggcctg cgaactgcgc gacggggaca agaagcgcta cgtcgggaag       180
     ggttgcctac aggcagtaaa gaatgtaaat gaggtaattg gccctgcgct tatcggcagg       240
     gacgagctta agcaagaaga attggacaca ctgatgcttc ggctcgacgg cactcccaac       300
     aagggtaagc tcggtgccaa cgctatcctc ggatgctcga tggccatcag caaagccgct       360
     gctgcggcga aaggtgttcc cctctaccgc taccttgcct ccctcgccgg tacgaaggaa       420
     ctgcgccttc ctgttccttg cttcaacgtg atcaacggtg gcaagcacgc cggaaacgcc       480
     ttacccttcc aggagttcat gatcgccccg gtgaaggcca cctcgtttag cgaggcgctg       540
     cgtatgggtt ctgaggtgta ccactccctc agaggcatca tcaagaagaa gtacggtcag       600
     gatgctgtga acgttggtga tgagggcggg tttgccccac cgatcaaaga cattaatgag       660
     ccgcttccta tcctcatgga ggccattgag gaggctggtc acaggggtaa gttcgccatc       720
     tgcatggact gcgctgccag tgaaacctat gacgaaaaga agcaacaata taatttaacc       780
     ttcaagagcc ccgaacccac ttgggtaacg gcagagcagt tgcgcgaaac gtactgcaaa       840
     tgggcgcacg actacccaat tgtcagtatt gaagaccctt acgatcaaga cgacttcgct       900
     ggtttcgctg gcatcactga ggctctgaag ggcaaaactc aaattgtcgg cgacgacctc       960
     actgtcacca acaccgaacg catcaagatg gccatcgaaa agaaggcctg caactctctt      1020
     ctactgaaaa tcaaccaaat cggtaccatc agcgaggcaa tcgcatcatc gaagttgtgc      1080
     atggagaatg gctggtcagt gatggtctct caccgcagtg gtgagacaga ggacacgtac      1140
     atcgcggacc ttgttgttgc tcttggttct ggtcaaatta aaactggtgc accgtgccgc      1200
     ggcgagcgca cagcaaaact caaccagctg ctgcgcattg aagaggaact tggtgcccat      1260
     gctaaatttg gctttcccgg ttggtcgtaa                                       1290
//