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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AF149311; SV 1; linear; mRNA; STD; PLN; 1623 BP.
XX
AC   AF149311;
XX
DT   25-OCT-1999 (Rel. 61, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   Rauvolfia serpentina raucaffricine-O-beta-D-glucosidase mRNA, complete cds.
XX
KW   .
XX
OS   Rauvolfia serpentina (serpentwood)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Rauvolfia.
XX
RN   [1]
RP   1-1623
RX   DOI; 10.1016/S0031-9422(00)00175-8
RX   PUBMED; 10975500.
RA   Warzecha H., Gerasimenko I., Kutchan T.M., Stockigt J.;
RT   "Molecular cloning and functional bacterial expression of a plant
RT   glucosidase specifically involved in alkaloid biosynthesis";
RL   Phytochemistry 54(7):657-666(2000).
XX
RN   [2]
RP   1-1623
RA   Warzecha H., Kutchan T.M., Stoeckigt J.;
RT   ;
RL   Submitted (09-MAY-1999) to the EMBL/GenBank/DDBJ databases.
RL   University of Mainz, Institute of Pharmacy, Staudinger Weg 5, Mainz 55099,
RL   Germany
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1623
FT                   /organism="Rauvolfia serpentina"
FT                   /strain="BENTH. ex KURZ"
FT                   /mol_type="mRNA"
FT                   /note="from cell suspension culture"
FT                   /db_xref="taxon:4060"
FT   CDS             1..1623
FT                   /codon_start=1
FT                   /product="raucaffricine-O-beta-D-glucosidase"
FT                   /function="hydrolyses the glucoalkaloid raucaffricine"
FT                   /EC_number="3.2.1.125"
FT                   /note="beta glucosidase; RG; part of the Ajmaline
FT                   biosynthesis pathway; belongs to family 1 of the glucosyl
FT                   hydrolases"
FT                   /db_xref="GOA:Q9SPP9"
FT                   /db_xref="HSSP:1CBG"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="UniProtKB/TrEMBL:Q9SPP9"
FT                   /protein_id="AAF03675.1"
FT                   /translation="MATQSSAVIDSNDATRISRSDFPADFIMGTGSSAYQIEGGARDGG
FT                   RGPSIWDTFTHRRPDMIRGGTNGDVAVDSYHLYKEDVNILKNLGLDAYRFSISWSRVLP
FT                   GGRLSGGVNKEGINYYNNLIDGLLANGIKPFVTLFHWDVPQALEDEYGGFLSPRIVDDF
FT                   CEYAELCFWEFGDRVKHWMTLNEPWTFSVHGYATGLYAPGRGRTSPEHVNHPTVQHRCS
FT                   TVAPQCICSTGNPGTEPYWVTHHLLLAHAAAVELYKNKFQRGQEGQIGISHATQWMEPW
FT                   DENSASDVEAAARALDFMLGWFMEPITSGDYPKSMKKFVGSRLPKFSPEQSKMLKGSYD
FT                   FVGLNYYTASYVTNASTNSSGSNNFSYNTDIHVTYETDRNGVPIGPQSGSDWLLIYPEG
FT                   IRKILVYTKKTYNVPLIYVTENGVDDVKNTNLTLSEARKDSMRLKYLQDHIFNVRQAMN
FT                   DGVNVKGYFAWSLLDNFEWGEGYGVRFGIIHIDYNDNFARYPKDSAVWLMNSFHKNISK
FT                   LPAVKRSIREDDEEQVSSKRLRK"
XX
SQ   Sequence 1623 BP; 473 A; 319 C; 386 G; 445 T; 0 other;
     atggcaactc agagcagtgc tgttatcgac tccaatgatg caaccagaat cagccgtagc        60
     gattttcctg ctgattttat aatgggaacg ggctcttctg cttatcagat tgaaggtggg       120
     gcgagagatg ggggtcgtgg cccgagcata tgggatactt tcacccacag acgacctgat       180
     atgatccggg gtggaactaa cggggatgtg gctgtggact cttaccattt gtacaaggaa       240
     gatgtcaaca tcctcaaaaa cttggggctt gatgcctacc ggttctcaat ctcatggtcc       300
     agagtgttgc caggtggaag actaagtggt ggcgtgaaca aggaagggat taattactac       360
     aataatctca ttgatggact cctagccaat gggattaaac catttgtaac tctcttccac       420
     tgggatgttc cccaagcctt agaagatgaa tatggtggct ttttgagtcc ccggattgtg       480
     gatgatttct gtgagtatgc ggagctttgt ttctgggaat tcggtgatcg ggtgaaacac       540
     tggatgacat tgaacgagcc atggacattt agcgttcatg gatatgcaac aggcctttat       600
     gcacccggtc gaggtcggac ttctccagag cacgtaaacc atcccactgt gcaacacaga       660
     tgttctactg tcgcaccaca atgcatttgc agcactggaa atccgggcac agaaccatat       720
     tgggttactc atcatctact tcttgctcat gctgctgctg ttgaattata caagaacaaa       780
     tttcagaggg gtcaagaagg acagattgga atttcacacg cgactcaatg gatggagcct       840
     tgggatgaga atagtgccag tgatgtggaa gctgcagcta gagctcttga ttttatgttg       900
     ggatggttca tggagcccat aacatctggt gattatccaa agagcatgaa aaaattcgtt       960
     ggatcacgac tgccaaagtt ttcacctgaa caatctaaga tgctaaaggg atcatatgac      1020
     tttgttggat tgaattacta tactgcttct tacgtgacta atgcatcaac aaattcttct      1080
     ggatcgaata atttcagcta taatactgat attcatgtta cctatgaaac tgacaggaat      1140
     ggtgtaccaa ttggtcccca gtctggttca gattggctac ttatttatcc agagggcatc      1200
     cggaaaatat tagtttacac caagaaaaca tacaatgttc ctctcatata tgtcacagag      1260
     aatggagttg acgatgtaaa gaatactaat ctaacacttt ctgaagctcg taaagattcg      1320
     atgagactga aatatctcca agaccatata ttcaacgtcc gccaagcaat gaatgatggt      1380
     gtaaatgtga agggctattt tgcatggtca ttattggata attttgaatg gggtgaaggg      1440
     tatggagtgc gttttggtat catccacatc gactataatg acaactttgc aagatacccc      1500
     aaagattcag cagtatggct catgaattct ttccacaaga acatctccaa acttcctgca      1560
     gtcaagagaa gtatcagaga ggacgacgaa gaacaagttt caagcaagag attaagaaag      1620
     tag                                                                    1623
//
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