 |
|
EBI DbfetchID AF148496; SV 1; linear; genomic DNA; STD; PRO; 18270 BP. XX AC AF148496; AF143810; L04642-L04643; U08463; U25434; XX DT 14-DEC-1999 (Rel. 62, Created) DT 22-DEC-2006 (Rel. 90, Last updated, Version 5) XX DE Pseudomonas putida transposon Tn5542, complete sequence. XX KW . XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OG Plasmid pHMT112 XX RN [1] RP 8542-12439 RX DOI; 10.1016/0378-1119(93)90343-2 RX PUBMED; 8344526. RA Tan H.M., Tang H.Y., Joannou C.L., Abdel-Wahab N.H., Mason J.R.; RT "The Pseudomonas putida ML2 plasmid-encoded genes for benzene dioxygenase RT are unusual in codon usage and low in G+C content"; RL Gene 130(1):33-39(1993). XX RN [2] RP 6856-8541 RX PUBMED; 8824602. RA Fong K.P., Goh C.B., Tan H.M.; RT "Characterization and expression of the plasmid-borne bedD gene from RT Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene RT dihydrodiol dehydrogenase"; RL J. Bacteriol. 178(19):5592-5601(1996). XX RN [3] RP 1-5110, 12440-18270 RX DOI; 10.1006/plas.1999.1442 RX PUBMED; 10686128. RA Fong K.P., Goh C.B., Tan H.M.; RT "The genes for benzene catabolism in Pseudomonas putida ML2 are flanked by RT two copies of the insertion element IS1489, forming a class-I-type RT catabolic transposon, Tn5542"; RL Plasmid 43(2):103-110(2000). XX RN [4] RP 5111-6855 RA Goh C.B.H., Tan H.M.; RT "Identification of a transposase A gene (tnpA) in Pseudomonas putida ML2"; RL Unpublished. XX RN [5] RP 8542-12439 RA Tan H.M.; RT ; RL Submitted (25-APR-1994) to the EMBL/GenBank/DDBJ databases. RL Microbiology, National University of Singapore, Lower Kent Ridge Rd. 0511, RL Singapore XX RN [6] RP 5111-6855 RA Goh C.; RT ; RL Submitted (29-JUN-1996) to the EMBL/GenBank/DDBJ databases. RL Microbiology, National University of Singapore, Lower Kent Ridge Rd. 0511, RL Singapore XX RN [7] RP 6856-8541 RA Tan H.M.; RT ; RL Submitted (05-OCT-1996) to the EMBL/GenBank/DDBJ databases. RL Microbiology, National University of Singapore, Lower Kent Ridge Rd. 0511, RL Singapore XX RN [8] RP 1-18270 RA Fong K.P.V., Tan H.M.; RT ; RL Submitted (05-MAY-1999) to the EMBL/GenBank/DDBJ databases. RL Microbiology, National University of Singapore, 10 Kent Ridge Crescent RL 119260, Singapore XX CC On or before Dec 10, 1999 this sequence version replaced CC gi:1398919, gi:786267, gi:474888. XX FH Key Location/Qualifiers FH FT source 1..18270 FT /organism="Pseudomonas putida" FT /plasmid="pHMT112" FT /strain="ML2" FT /mol_type="genomic DNA" FT /db_xref="taxon:303" FT repeat_region 1..18270 FT /mobile_element="transposon:Tn5542" FT gene 5111..6865 FT /gene="tnpA" FT CDS 5111..6865 FT /codon_start=1 FT /transl_table=11 FT /gene="tnpA" FT /product="TnpA" FT /note="transposase A" FT /db_xref="GOA:Q51984" FT /db_xref="InterPro:IPR002560" FT /db_xref="UniProtKB/TrEMBL:Q51984" FT /protein_id="AAB03184.1" FT /translation="MDVTHAPTMKVAREVADDYLNAYYEAIKAGKTRHQIDGVQPEVLQ FT AILAHALMSQRMLVSFNSIGQMNCRLIPGDASVVSFAKDDYFNLAQHVPMERLPDMLDA FT LNKRVACHLGLSASPSPRLFEESDVSDLTIPQTLLHPLKAKVVGFHEEENEYSFQVEYP FT DPTVCPNCGVCGEVVRFGKKQVKFRDLPLHGRWVTLWLIRRRFVCRACNTTFSPALPEM FT AENNRMTQRLAEYIIKAAVGRTNSDVGREVGVNESIIRALFQSHYEAQKHRYTPSAPRV FT LGIDELYLQRTYRCVLTNIEEETIIDLLESRTKPQVTAYLSRLKDRKDIEIVCMDMWNP FT YREVAKALMPQAAIVVDKFHIQRMANEVMEKIRRGIKAGLSQHRRRALKGDRKLMLMRE FT RDLTPMSQMVVETWTNQFPELGAAYRLKEEFMNIWLLTDEVDAREEYQRWQSSLTPEQR FT PQWAPLTTAMSNWEAEIFAYFGDGQRQTNAFTESINRQMKDLNRVTRSMSFEMFRAKML FT FSVDHKMKRKEKPRRESPFEIGYGVPTEADLARLAPTMLDMGVPIGKLLERFLEPPINI FT TPPPSEAG" FT repeat_region 5263..7161 FT /mobile_element="insertion sequence:IS1489V1" FT gene 7002..8099 FT /gene="gldA" FT CDS 7002..8099 FT /codon_start=1 FT /transl_table=11 FT /gene="gldA" FT /product="GldA" FT /EC_number="1.1.1.6" FT /note="glycerol dehydrogenase" FT /db_xref="GOA:P50173" FT /db_xref="HSSP:1KQ3" FT /db_xref="InterPro:IPR016205" FT /db_xref="UniProtKB/Swiss-Prot:P50173" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC44426.1" FT /translation="MDRAIQSPGKYVQGADALQRLGDYLKPLADSWLVIADKFVLGFAE FT DTIRQSLSKAGLAMDIVAFNGECSQGEVDRLCQLATQNGRSAIVGIGGGKTLDTAKAVA FT FFQKVPVAVAPTIASTDAPCSALSVLYTDEGEFDRYLMLPTNPALVVVDTAIVARAPAR FT LLAAGIGDALATWFEARAASRSSAATMAGGPATQTALNLARFCYDTLLEEGEKAMLAVQ FT AQVVTPALERIVEANTYLSGVGFESGGVAAAHAVHNGLTAVAETHHFYHGEKVAFGVLV FT QLALENASNAEMQEVMSLCHAVGLPITLAQLDITEDIPTKMRAVAELACAPGETIHNMP FT GGVTVEQVYGALLVADQLGQHFLEF" FT gene 8811..10163 FT /gene="bedC1" FT CDS 8811..10163 FT /codon_start=1 FT /transl_table=11 FT /gene="bedC1" FT /product="BedC1" FT /note="oxygenase alpha subunit" FT /db_xref="GOA:Q07944" FT /db_xref="HSSP:1O7N" FT /db_xref="InterPro:IPR001663" FT /db_xref="UniProtKB/Swiss-Prot:Q07944" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA17758.1" FT /translation="MNQTETTPIRVRKNWKTSEIETLFDEQAGRIDPRIYTDEDLYQLE FT LERVFARSWLLLGHETHIRKPGDYFTTYMGEDPVVVVRQKDASIAVFLNQCRHRGMRIC FT RSDAGNAKAFTCSYHGWAYDTAGNLINVPYEAESFACLDKKEWSPLKARVETYKGLIFA FT NWDENAIDLDTYLGEAKFYMDHMLDRTEAGTEVIPGIQKWVIPCNWKFAAEQFCSDMYH FT AGTTAHLSGIIAGLPEDLELADLAPPKFGKQYRASWGGHGSGFYIGDPNMMLAMMGPKV FT TSYLTEGPAAEKAAERLGSIERGTKIMLEHMTVFPTCSFLPGVNTIRTWHPRGPNEVEV FT WAFTVVDADAPDDIKEEFRRQTLRTFSAGGVFEQDDGENWVEIQHILRGHKARSRPFNA FT EMSMGQTVDNDPIYPGRISNNVYSEEAARGLYAHWLKMMTSPDWEALKATR" FT gene 10294..10857 FT /gene="bedC2" FT CDS 10294..10857 FT /codon_start=1 FT /transl_table=11 FT /gene="bedC2" FT /product="BedC2" FT /note="oxygenase beta subunit" FT /db_xref="GOA:Q07945" FT /db_xref="InterPro:IPR000391" FT /db_xref="UniProtKB/Swiss-Prot:Q07945" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA17759.1" FT /translation="MIDSVNRADLFLRKPAPVALELQNEIEQFYYWEAKLLNDRRFDEW FT FALLAKDIHYFMPIRTTRIMRDSRLEYSGLRDYAHFDDDATMMKGRLRKITSDVSWSEN FT PASRTRHIVSNVMIIPTEVEGEYEISSTFIVYRNRLERQLDIFAGERRDRLRRNKGEAG FT FEIVNRTILIDQSTILANNLSFFF" FT gene 10866..11189 FT /gene="bedB" FT CDS 10866..11189 FT /codon_start=1 FT /transl_table=11 FT /gene="bedB" FT /product="BedB" FT /note="ferredoxin" FT /db_xref="GOA:Q07947" FT /db_xref="HSSP:1FQT" FT /db_xref="InterPro:IPR017941" FT /db_xref="UniProtKB/Swiss-Prot:Q07947" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA17760.1" FT /translation="MTWTYILRQSDLPPGEMQRYEGGSEPVMVCNVDGEFFAVQDTCTH FT GDWALSEGYLDGDVVECTLHFGKFCVRTGKVKALPACKPIKVYPIKIEGDEVHVDLDNG FT ELK" FT gene 11189..12421 FT /gene="bedA" FT CDS 11189..12421 FT /codon_start=1 FT /transl_table=11 FT /gene="bedA" FT /product="BedA" FT /note="reductase" FT /db_xref="GOA:Q07946" FT /db_xref="HSSP:1M6I" FT /db_xref="InterPro:IPR001327" FT /db_xref="UniProtKB/Swiss-Prot:Q07946" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA17761.1" FT /translation="MANHVAIIGNGVAGFTTAQALRAEGYEGRISLIGEEQHLPYDRPS FT LSKAVLDGSFEQPPRLAEADWYSEASIEMLTGSEVTDLDTQKKMISLNDGSTISADAIV FT IATGSRARMLSLPGSQLPGVVTLRTYGDVQLLRDSWTPNTRLLIVGGGLIGCEVATTAR FT KLGLSVTILEAGDELLVRVLGRRIGAWLRGLLTEQGVQVELKTGVSGFSGEGQLEKVMV FT NDGRSFIADNALICVGADPADQLARQAGLECDRGVVVDHRGATSAKGIFAVGDVATWPL FT HSGGKRSLETYMNAQRQATAVAKAILGKEVSAPQLPVSWTEIAGHRMQMAGDIEGPGEY FT VLRGTLGIGSALLFRLLDGRIQAVVAVDAPRDFALANRLVEAQVIIEPEKLADVSNNMR FT DIVRANEGNQK" FT gene 15237..16970 FT /gene="tnpA" FT CDS 15237..16970 FT /codon_start=1 FT /transl_table=11 FT /gene="tnpA" FT /product="TnpA" FT /note="transposase" FT /db_xref="GOA:Q9R9T8" FT /db_xref="InterPro:IPR002560" FT /db_xref="UniProtKB/TrEMBL:Q9R9T8" FT /protein_id="AAF16415.1" FT /translation="MKVAREVADDYLNAYYEAIKAGKTRHQIDGVQPEVLQAILAHALM FT SQRMLVSFNSIGQMNCRLIPGRCVCCELCQGRLLQSCPARPDGATARHAGCAEQASSLP FT SWRFQRALAPRLFEESDVSDLTIPQTLLHPLKAKVVGFHEEENEYSFQVEYPDPTVCPN FT CGVCGEVVRFGKKQVKFRDLPLHGRWVTLWLIRRRFVCRACNTTFSPALPEMAENNRMT FT QRLAEYIIKAAVGRTNSDVGREVGVNESIIRALFQSHYEAQKHRYTPSAPRVLGIDELY FT LQRTYRCVLTNIEEETIIDLLESRTKPQVTAYLSRLKDRKDIEIVCMDMWNPYREVAKA FT LMPQAAIVVDKFHIQRMANEVMEKIRRGIKAGLSQHRRRALKGDRKLMLMRERDLTPMS FT QMVVETWTNQFPELGAAYRLKEEFMNIWLLTDEVDAREEYQRWQSSLTPEQRPQWAPLT FT TAMSNWEAEIFAYFGDGQRQTNAFTESINRQMKDLNRVTRTMSFEMFRAKMLFSVDHKM FT KRKEKPRRESPFEIGYGVPTEADLARLAPTMLDMGVPIGKLLERFLEPPINITPPPSEA FT G" FT repeat_region 15366..16971 FT /mobile_element="insertion sequence:IS1489V2" XX SQ Sequence 18270 BP; 4264 A; 4924 C; 5148 G; 3934 T; 0 other; catgcaagca tgacttgagc gttctgtcca accagcgatc acgctgaaca gacgctctga 60 tggcctgaaa aaaaatacaa gatcaggagg taccatgaat cgcccatccc tcatccggtc 120 cactcggccc cgcgcgcggc tgaagtagga ctctactctc cccataaatc tatctctcga 180 atggcgactc ggcggcgaca gcttgctggg cgcacttcgg ggtctgtcag acgtgttccg 240 ggcttgcgtg caagttctga gcatcactat gtaatgcctg gagaataaga aatgactacc 300 tccaacagct tcgataccga tgttttggtc atcggcaccg gccccgcagg tgcaactact 360 gccttggcac tcgccactta cggtgtccga gtcaaaatgg tcaccatgtt caattggttg 420 gccaactctc cacgagcgca cattactaac cagcgtgcca tggaggttgt gcgtgacttg 480 ggcttggaag acgaggtggt gagagctgct accccatggg atcagatggg tgacacgttg 540 ttcaccacaa gcctggcggg tgaagaaatc gcccgcatgc gtacttgggg taccggtgac 600 aagcgcaaag gcgattacgt tcaaggtagc ccctgcacca tgctggacct gatccagccg 660 gagatggaag caatcctgct caggtcagct tcaacaccga gtacatgagt cacgaggaag 720 actccgaagg tgtaaccgct ctcttgaaag acgtgctcac tggtgcggag taccaggtgc 780 gagccaaata cttggtcggt gccgacggag ctaactccaa agtcgccaaa gaaattggcc 840 tgccgatcga aggtgagatg gcacgggcag gtactgctta tgtgatcttc aatgccgacc 900 taagcaagta tgttgagcac cgtcctagca ttttgcagtg gattgccaca ccagaggctg 960 gctttgggga gatcggcatg ggcttgttgc gcgctatccg cccgtgggat cagtggattg 1020 ccgggtgggg ctttgacatg accaaaggtt ccccagacct ctcgccagaa gcggttctgc 1080 agaaaattcg cttgttggtc ggtgaccctg atttggaagt tcagattgaa cgcatgtcga 1140 cttggtacgt aaaccaggca catgccacct cgtattcacg aggtcgtgtg ttctgtgccg 1200 gtgacgcggt ccaccgtcat ccgccatcca gtgggctggg ttcgaatacc tgtatgcagg 1260 acggtttcaa ccttgcgtgg aagttggcct atgtaattca aggcttcgcg ggcccagagc 1320 tacttggaag ctatacggct gagcgtgcgc ctgtaggtaa gcagattgtg gcgcgtgcga 1380 atcagtcgcg taaagattat gcgttgttga aggaatgctt caacttcggt aagggtgtcg 1440 acactatcgc caagctcctg gagcgaatgc gcgatccaag cccagctggg gtagaggtcc 1500 gcatgaatct gcagaaagcg ctcgaaatca agagtcgcga attcaacgcc caaggtgttg 1560 agttgaatca gcgctatgaa tcctcagcag tgatggctga cccaagtgat ggcgaggaag 1620 tatgggctcg cgacaaagag ctctacctgc aggcaacgac tcgtcctggt gcgaagctcc 1680 cgcatgcttg gctggtaaat gagactggcc atcgagtatc tacgctcgac gttgtgggta 1740 aagggaaaat gacgttgatc accggtcttg cggggcaggc gtgggttgat gcggctaagc 1800 gcttggagtt gccgttccta aaaatcgtca tcacgggtgc caaaggcact gcagacctct 1860 actgcgattg gcagtctgtg cgcgaagtcc atgaagccgg cgccatcctt gtgcggcctg 1920 atggctatgt tgcatggcgt caaagcgatg ctgtctggac tgccgatgag gctgtggtcc 1980 aactccagca agcacttcag aacgtgctcg ggtaagtgtt tctctgggcc cggcttcggc 2040 cgggtctttt ttcatctggg gaatgattcc tcagccataa agagtgccgt catccgcgtg 2100 gtcccacgcg ttcctagcac ggctaccaaa actgaatgtt cctgatcccc gatgtcagca 2160 atgctttgct gatgagtgtt gttctgtggg tgatgaactg ccttaagtgc gatgaggtta 2220 tctatggcta aggccgccga tgttgttgtg cagtgcttgg aaaacgaagg cgtcgaatat 2280 gtgttcggca ttccgggtga ggaaaacctt gacctgctgg aatccttgcg caagtcgaaa 2340 gatcaagttg gtcctgaccc gccacgagca gtccgcgggc ttcatggccg ccacctacgg 2400 ccgcctgacc ggcaagaccg gcgtcagcct gtcgaccctg ggccctggcg cgaccaacct 2460 ggtcaccgcc agtgcctacg cctacctggg cggcatgccg atgatgatga tcactggcca 2520 gaagccgatc aagaagtcca agcagggtcg cttccagatc attgacgtgt gcggcatgat 2580 ggcccccatc accaagtaca cccaccagtt cgcctcggcc gacaacatcc cggcccgtat 2640 gcgcgaagcg ttccgcctgg ctgaagaaga aaagccaggg gccgtgcacc tggagctgcc 2700 ggaagatatt gctgccgagc agactgacgc tctaccgatc cctccaagcc tgcatcgccg 2760 cccgctggcc gagcacaagg ctatcgaagc ggcggttgag aaactgcaga acgcgcgcag 2820 cccaatcctg gtgatcggcg ccggtgccaa ccgcaagatg accgccaagg tcctcaagca 2880 gctgatcgac aagaccggca tcccgttcat caccacccag atggcaaaag cgtggtcgat 2940 gagcgccacc cgcgcttcct cggcaacgct gcgctttcgt ccggtgactt cgtccaccgc 3000 gccattgaag cagccgacct gatcatcaac atcgggcacg acgtgattga agaagccgcc 3060 aatcttcaag gtccgtggcg gcaccgaagt catccacatc aaccttcgct ccgccgaagt 3120 ccaatgcctg gtacttccgc aggtcgaagt gatcggcgac atagcaaacg ccgtctggca 3180 gatcagcgaa gccctgaacg acacttcgca ctgggacttc acccgcctga tggccatccg 3240 tgaagccaac gaagcgcaaa tcgccgaagg cgctgacgat gaccgcttcc cagtctaccc 3300 acagcgcctg gtcgccgaca tccgccgcgt gctgccctcc gaaggcatcg tcgccttgga 3360 caacggcatc tacaagatct ggtttgcccg caactacaag cgcacaagcc gaatacggtg 3420 ctgctggata acgccttggc caccatgggc gctggcttgc catcggccat gggcgcgcac 3480 ctggtgcatc cggaccgccc ggtgattttc ggtctgcggt gacggcggtt tcatgatgaa 3540 cagccaagga gctgggaaac ggctggtacg tctgggcatg cacatcaccg tggtatcctg 3600 cgtgacgacg gctacggcat gatccgctgg aagcaggcca acatgggctt caccgatttc 3660 ggcctggact acggcaatcc ggacttcgtc aagtacgccg aagcctacgg cgcccacggg 3720 tatcgggtgg agagtgcaga tgcactgcag ccgctgctcg atcactgtat ctccaatccg 3780 ggcgtgcacg tgatcgactg cccggtcgac tacagcgaga acgaccgcat cctgaacagc 3840 gagttgcgtg agcgagcact ggcaatctaa aagaacctct ctcgacattt agaatgtccg 3900 acagcttttg cgacaatcgc ggaacgccta ttggtgctgc ttgagtgagg cggaagaagg 3960 tcagtcggct tctttcgccg caccataaag gccattttgc atttgaaatt gactccagag 4020 gcagtagtcg cgatattcgt ggagacaaaa taacaatgaa gtctatacgc tcgtcaattg 4080 ctatctattc tggctccata ctgctggttg cagttctgtc gtttttggct gcagtctaca 4140 aaattaattc tgaggtcttt gagctggtca gtcatcgagt tcaaggattg caggaaaaca 4200 atatcgagaa atatttgttt gcaaacgctg gtcagcaagc cgaaaagatt tcggcgggtt 4260 tggctaacgc agcattggta acaaaagctc ttgcagatgt cgtgtcatct caatctcagc 4320 aagaatcgct ggcaggggta agccggaaag ccctaacaga catacttaaa agtttcgggt 4380 attcataaaa ggttgttgat aggggtctga tgccctcctg ctacgaatca tttttgattt 4440 cgcggaaagg tgttgtcact ccatcttttt ggtgttagag tccgtctcag gtgctgaaca 4500 caccttgtca ggcggaagcc gctcccgaaa gtcaaagcgg ctttttcacg tccgtaagtt 4560 ttgcgttgcc cccgatgcgg gacaatgcac gcttatggcc gggtagcgcg cagctataca 4620 agacccttcg ggggaaatct gcgggcggtc ctgacaccgt gttcaagtac ccggccgcct 4680 ctctgaacaa gaggcgaatt tgaacgaatg tcaggaggcc aacatggctg ctcaatccaa 4740 tctgctccca aagggtgtcg ctactaacga cacacttcat caaaccttca acttcgagaa 4800 cctgccggtt cgggcaacaa accgaaacgg agaaatctgg ttcgtagccg ctgacgtgtg 4860 tgcggcgctg gagatcacca acccccggca tgctgtcagt cgccttgacg atgacgaaaa 4920 gggtgtcatc actgtggaca cccttggcgg cccccaggag acggtcatca tcagcgaatc 4980 gggtctctac agcctgacgc tgaccagtcg caaagaagag gccaagcggt tcaagcggtg 5040 gattacccac gaggttcttc cagccatccg caagacgggg ggttaccaga agcacgaaac 5100 cacgcccgcc ttggatgtga cccacgcccc gactatgaag gttgcgcgcg aggtagccga 5160 cgactacctt aatgcctact atgaggcgat caaggcgggt aaaacacgcc atcaaattga 5220 cggcgtacag cccgaagtgc tccaagccat cctggcgcat gcgctgatga gtcagcggat 5280 gttggtttcc ttcaattcca tcggccaaat gaactgccgc ctgattcccg gcgatgcgtc 5340 tgttgtgagc tttgccaagg acgactactt caatcttgcc cagcacgtcc cgatggagcg 5400 actgcccgac atgctggatg cgctgaacaa gcgagtagct tgccatcttg gcctttcagc 5460 gagcccttcg ccgcgactct tcgaggaatc tgacgtgagt gatctgacca tcccgcaaac 5520 gctattgcac ccgctcaagg ctaaggtggt ggggtttcat gaggaagaga acgaatacag 5580 cttccaggtg gaataccccg atcccacggt ctgcccgaac tgcggggtgt gcggcgaggt 5640 ggtcaggttc ggcaagaagc aggtgaagtt ccgtgacctg ccgctgcatg ggcggtgggt 5700 cacgctctgg ctcatccgcc gacgcttcgt gtgtcgggcg tgcaacacga cattcagccc 5760 cgccttgcct gaaatggctg agaacaaccg catgacacag cggctggccg agtacatcat 5820 caaggccgcc gtggggcgca ccaacagcga cgtgggccgc gaggtgggcg tgaatgagtc 5880 gatcatccgc gccctgttcc agagccacta cgaggcacag aaacaccgct acacgccatc 5940 tgcgccccgt gtgctgggca ttgatgagct gtacctgcaa cgcacctacc gctgcgtgct 6000 gaccaatatc gaggaggaaa ccatcatcga cctgttggag tcgcgcacga agccacaggt 6060 cacggcctac ctatctcgtc tcaaagaccg aaaggacatt gagatcgtct gcatggatat 6120 gtggaacccg taccgcgagg tggccaaggc gctgatgccg caagccgcca ttgtggtgga 6180 caagttccac atccagcgca tggccaacga ggtcatggag aagatcagaa gaggcatcaa 6240 ggctgggctg tcgcaacatc gccgccgtgc gctcaaaggc gaccgcaagc tgatgctgat 6300 gcgagagcgc gacttgacgc ccatgagcca aatggtggtg gagacctgga cgaaccagtt 6360 ccccgagctg ggcgcggcct accgcctcaa ggaagagttc atgaacatct ggctgctgac 6420 cgacgaagtg gacgccagag aggaatacca gcgctggcaa tccagcctga caccggagca 6480 gcggccccaa tgggcaccgc ttactaccgc aatgagcaac tgggaggcgg aaatcttcgc 6540 ctacttcggt gacgggcaac gccagaccaa tgccttcacc gagtcgatca accggcaaat 6600 gaaagacctg aacagggtca cgcggagcat gtccttcgag atgttccgag ccaagatgct 6660 gttctcagtc gatcacaaga tgaagcgcaa ggaaaagccg cgccgggaat cgccctttga 6720 aatcggctac ggcgtcccta cggaagccga ccttgcaagg ctggcaccca ccatgctcga 6780 catgggggtg cccatcggca agctgctgga acgcttcttg gagccgccca tcaacatcac 6840 cccaccacct tcggaagccg ggtagaaact cccttcatca accggctttt acgaataccc 6900 aaaggaactt catcgactca aatctctatt tgcaacagtg ccggcaatgt gcaacagtgc 6960 cggcaatgtg caacaatgtg caatttcaag aaggagtttc catggaccgc gccattcaat 7020 cacccggcaa atatgtacaa ggggccgatg cgctgcaacg gctgggggac tacctcaagc 7080 cgctggcgga cagctggctg gtgattgccg acaagttcgt gctgggcttt gccgaagaca 7140 ccatccgcca aagcctcagc aaggccgggc tggccatgga catcgtcgcc ttcaacggcg 7200 aatgctcgca gggcgaggtc gatcgcctgt gccaactggc cacgcaaaac gggcgcagcg 7260 ccatcgtcgg cattggtggc ggcaagacgc tggacaccgc caaggccgtg gcctttttcc 7320 agaaagtgcc cgtggccgtg gcccccacca tcgcctccac cgacgcgccc tgcagcgcgc 7380 tgtcggtgct ctataccgat gaaggtgagt tcgaccgcta tctgatgctg cccaccaacc 7440 ccgccctggt ggtggtggac accgccatcg tcgcccgtgc accggcgcgg ctgttggcgg 7500 ccggcattgg tgatgccctg gccacctggt tcgaggcgcg tgccgcatcg cgcagcagcg 7560 ctgccaccat ggccggcggc ccggccacgc agaccgcact gaacctggcc aggttctgct 7620 acgacaccct gctggaagag ggtgaaaaag ccatgttggc cgtgcaggcc caggtggtga 7680 cgccggcgct ggagcgcatc gtcgaggcca acacctatct gagcggggtc gggtttgaaa 7740 gcggtggcgt ggccgccgcc cacgcggtgc acaacggcct gaccgccgtg gccgaaaccc 7800 accacttcta ccacggcgaa aaagtggcgt ttggcgtgct ggtgcaactg gcgctggaaa 7860 acgcctccaa cgcggaaatg caggaagtga tgtcgctgtg ccacgccgtg ggcctgccca 7920 tcacgctggc gcagctggac attaccgaag acatccccac caagatgcgc gccgtggccg 7980 agctggcctg cgccccaggc gagaccatcc acaacatgcc cggcggcgtg acggtggagc 8040 aggtctatgg cgcgctgctg gtggcggacc agctggggca gcattttctg gagttttgag 8100 gcccccctga gtcgcctacg gcccttttcc ccccccccca gggggacgac agcctcgctg 8160 cggggcggcc cttgctcgct gtctctggcc tgggctccgc cggttttatg cgctggggct 8220 tgatgggggc tgggcgatgt gcaagccata cccgtgggca cgtcatgtca ccttcccccc 8280 gctctcgcat tgctacgcaa cgcgggcagg gggacgacac cagcgcggcg gggcggccct 8340 tgcgcggtgt ctctggcttt ggccccgcta gttttatgcg cagggggtct gatggggact 8400 gggcgaggtg caagccatat ccatgggcac gttatgtcac ctttcccccg cgctttgctc 8460 gcgggctggg ggacgacacc agcgcggcgg ggcggccttt gcgcggtgtc tctggcttgg 8520 ggcgcgccgg ctttatgcgt aggaggtctg atggggactg ggcgaggtgc agaccatatc 8580 cgtgggtacg tcatgtcccc ttcgggcaag cacatcaaga aaccaatgtc atcaaagccc 8640 tcccctttcg ggggagggtg tatagaccgg ggacatggtg aacaccttaa tcaacagggt 8700 tttcggtctc taagcacttt taaaaaggaa cttcatcgac tcaaatctct atttgcaaca 8760 gtgccacctg taattacccg ctatccacta ctttaaaaag tgagaagaca atgaatcaga 8820 cagaaacaac acctattaga gtgcgcaaaa attggaagac cagtgagata gaaacgctct 8880 ttgatgagca agctggacgt atcgatccgc gcatttatac cgatgaggat ctgtaccaac 8940 tcgaactaga acgtgtattt gctcggtcat ggctcctatt ggggcatgaa actcacattc 9000 gtaaaccagg tgattatttc acgacctata tgggtgaaga tcctgtcgtg gtcgtaagac 9060 agaaggatgc cagtatcgct gtgttcctga accagtgccg ccatcgtggt atgcgtatct 9120 gtcgttcgga tgctggaaac gcgaaggcat ttacttgtag ttaccatggg tgggcttacg 9180 atactgctgg caatcttatt aatgtgcctt acgaggccga atccttcgcc tgcttagaca 9240 agaaggaatg gagtccactg aaggctcgag tggaaaccta caaaggtctg atttttgcca 9300 actgggatga aaacgccata gaccttgata catatctcgg cgaggcgaag ttctacatgg 9360 accacatgct cgaccgtact gaggcaggca ctgaggtgat tccaggtata cagaagtggg 9420 ttattccctg taactggaaa ttcgcagcag agcagttttg tagtgacatg taccatgccg 9480 ggactacagc acatttatct ggaatcattg ctggtctgcc agaagatctt gagttggctg 9540 atcttgcacc gccgaaattt ggcaagcagt accgtgcatc atggggtggg catggcagtg 9600 gcttctatat tggcgacccc aacatgatgc ttgccatgat ggggccgaag gtcaccagct 9660 acttgaccga aggccccgcg gcggaaaagg cggccgagcg tctgggtagt atagagcgcg 9720 gcacgaaaat catgcttgag cacatgactg tctttcctac gtgttccttc ctcccaggtg 9780 tcaatacgat ccgaacatgg catccacgcg ggccgaacga ggttgaagtg tgggcattta 9840 cagtcgtcga tgctgatgct ccagatgata ttaaggaaga gtttcgtcgt cagacactac 9900 gtaccttctc tgccggtggt gtattcgagc aggatgacgg cgagaactgg gttgaaatcc 9960 agcatattct gcgaggtcat aaggcacgta gccgtccatt caatgctgag atgagtatgg 10020 ggcaaaccgt tgataacgat ccaatttacc ctggtcgtat atctaacaac gtgtatagcg 10080 aagaagctgc tcgtggacta tatgcacatt ggctgaaaat gatgacctca ccagactggg 10140 aagcattaaa ggcgacgcgt taaacctaac gacagctagc gccacatcgt ggtgccagtt 10200 agaagccgca tttgattcat aaccaaattg gatgcggtgg actatccatt tgaaatctac 10260 acggaatgat tacgattcca aaaaggagtg accatgattg attcagtcaa cagagctgac 10320 ctctttcttc gcaaacctgc accggtagca cttgaactgc aaaatgaaat tgagcagttc 10380 tactattggg aagctaagct tctcaatgat cgccgctttg atgaatggtt cgcactactt 10440 gccaaagaca ttcactactt catgcctatc cgcaccacac gaatcatgcg tgattcacgc 10500 cttgaatatt cgggcttgcg agactatgca cattttgatg atgacgccac aatgatgaaa 10560 ggacgtttgc gtaagattac ttctgacgta agttggtccg agaatcctgc gtcaagaaca 10620 cgacatattg tgagcaatgt gatgatcatt cccacagaag tagaaggaga atacgaaatc 10680 tccagtacct tcatcgtgta ccgcaatcgc ttggaaagac aacttgatat ctttgctgga 10740 gagcgtcgcg acagattgcg tcgtaacaag ggtgaagctg gattcgagat agtcaatcgg 10800 acaatcctga tcgaccaaag caccatccta gccaataacc tcagtttctt cttctaggag 10860 atgtcatgac ttggacatat atactgcggc aaagtgattt gccacctggt gaaatgcaac 10920 gctatgaagg tggatcagaa ccagtgatgg tctgtaacgt cgatggtgag tttttcgcag 10980 ttcaggatac ctgcacgcat ggagactggg cattatcaga gggatacctt gatggtgatg 11040 tcgtcgaatg tacgttgcat tttgggaagt tctgtgtgcg aactgggaaa gtgaaagcgt 11100 tgcctgcttg taaacctatc aaagtatacc ctatcaagat agaaggtgat gaggtacacg 11160 ttgatcttga caatggggag ctaaagtgat ggctaatcat gttgcaatca tcggtaatgg 11220 cgtagctggg ttcaccaccg cgcaagccct tcgtgccgaa ggttatgaag gacgaatctc 11280 actgattggg gaagaacagc atcttcccta tgaccgacca tccttgtcta aggccgtcct 11340 tgatggaagc tttgagcagc cacctagact ggccgaggca gattggtaca gtgaagccag 11400 catcgagatg ctgactggct cagaagttac tgacctagat acacagaaaa aaatgatcag 11460 tttgaatgat ggcagcacga tttctgctga tgctatcgtt attgcaactg gaagtcgagc 11520 acggatgttg tctttacccg gcagtcaatt gcctggtgtc gtgacattac gcacttatgg 11580 tgatgtgcag ttattacgtg atagctggac acccaatact cggttgctca ttgtaggcgg 11640 tgggttgatt ggttgtgagg tagcaacaac ggctcgcaag cttggtcttt ctgtcacgat 11700 ccttgaagct ggtgatgaac tgttggttcg tgttcttgga cgacgtatcg gtgcttggct 11760 acgtggtttg ttaactgaac agggtgtgca ggtagaacta aaaactggag tttcaggttt 11820 ttcaggcgaa ggtcagcttg aaaaagtgat ggtgaatgat gggcgtagct ttattgctga 11880 taacgcactt atctgcgtag gggcagatcc tgcagatcaa ctcgcacgtc aagccggttt 11940 ggaatgtgat cgtggtgtcg ttgttgatca tagaggtgcg acatctgcta aaggcatatt 12000 cgcggtcggg gatgtagcca cttggccact tcattcaggc ggaaagcgct cgcttgaaac 12060 ctatatgaat gctcagcgcc aagccacagc agttgccaaa gccattctag gaaaagaagt 12120 atcagcaccg caattgccag tgtcatggac ggagattgcc gggcatcgaa tgcaaatggc 12180 tggtgatatc gaaggaccgg gtgaatatgt tttgcgcgga accttaggca ttggctctgc 12240 tttattgttc cgtctgctag atggacggat tcaagcggtt gtagcggttg atgcacctcg 12300 tgatttcgca ctcgcaaatc gattagtgga agctcaagtc ataattgagc cagaaaagct 12360 ggcagatgtg tcaaataata tgcgcgatat tgttcgtgcg aatgaaggga atcaaaaatg 12420 aacttaaaga tgagtaattt tggggtattc ataaaaggtt gttgataggg gtctgatgcc 12480 ctcctgctac gaatcatttt tgatttcgcg gaaaggtgtt gtcactccat ctttttggtg 12540 ttagagtccg tctcaggtgc tgaacacacc ttgtcaggcg gaagcgtccc gaaagtcaaa 12600 gcgctttttc acgtccgtaa gttttgcgct tgcccccgat gccgggacaa tgcacgctta 12660 tggccgggta gcgcgcagct atacaagacc cttcggggga aatctgcggg cggtcctgac 12720 accgtgttca agtacccggc ccgtcctctc tgaacaagag gcgaatttga acgaatgtca 12780 ggaggccaac atggctgctc aatccaatct gctcccaaag ggtgtcgcta ctaacgacac 12840 ccttcatcaa accttcaact tcgagaacct gccggttcgg gcaacaaacc gaaacggaga 12900 aatctggttc gtagccgctg acgtgtgtcg cgcgctggag atcaccaacc cccgcatgct 12960 gtcatcgcct tgacgatgac gaaaagggtg tcatcactgt ggacaccctt ggcggccccc 13020 aggagacggt catcatcagc gaatcgggtc tctacagcct gacgctgacc agtcgcaaag 13080 aagaggccaa gcggttcaag cggtggatta cccacgaggt tcttccagcc atccgccaag 13140 acggggggtt accagaagca cgaaaccacg cccgccttgg atgtgaccca cgccccgact 13200 atgaaggtgc gcgcgaggta gccgacgact accttaatgc ctactatgag cgatcaaggc 13260 gggtaaaaca cgccatcaaa ttgacggcgt acagcccgaa gtgctccaag ccatcctggc 13320 gcatgcgctg atgagtcagc ggatgttggt ttccttcaat tccatcggcc aaatgaactg 13380 ccgcctgatt cccggcgatg cgtctgttgt gagctttgcc aaggacgact acttcaatca 13440 cttaaagatg agtaattttg gggtattcat aaaaggttgt tgataggggt ctgatgccct 13500 cctgctacga atcatttttg atttcgcgga aaggtgttgt cactccatct ttttggtgtt 13560 agagtccgtc tcaggtgctg aacacacctt gtcaggcgga agcgtcccga aagtcaaagc 13620 gctttttcac gtccgtaagt tttgcgcttg cccccgatgc cgggacaatg cacgcttatg 13680 gccgggtagc gcgcagctat acaagaccct tcgggggaaa tctgcgggcg gtcctgacac 13740 cgtgttcaag tacccggccc gtcctctctg aacaagaggc gaatttgaac gaatgtcagg 13800 aggccaacat ggctgctcaa tccaatctgc tcccaaaggg tgtcgctact aacgacaccc 13860 ttcatcaaac cttcaacttc gagaacctgc cggttcgggc aacaaaccga aacggagaaa 13920 tctggttcgt agccgctgac gtgtgtcgcg cgctggagat caccaacccc cgcatgctgt 13980 catcgccttg acgatgacga aaagggtgtc atcactgtgg acacccttgg cggcccccag 14040 gagacggtca tcatcagcga atcgggtctc tacagcctga cgctgaccag tcgcaaagaa 14100 gaggccaagc ggttcaagcg gtggattacc cacgaggttc ttccagccat ccgccaagac 14160 ggggggttac cagaagcacg aaaccacgcc cgccttggat gtgacccacg ccccgactat 14220 gaaggtgcgc gcgaggtagc cgacgactac cttaatgcct actatgagcg atcaaggcgg 14280 gtaaaacacg ccatcaaatt gacggcgtac agcccgaagt gctccaagcc atcctggcgc 14340 atgcgctgat gagtcagcgg atgttggttt ccttcaattc catcggccaa atgaactgcc 14400 gcctgattcc cggcgatgcg tctgttgtga gctttgccaa ggacgactac ttcaatcact 14460 taaagatgag taattttggg gtattcataa aaggttgttg ataggggtct gatgccctcc 14520 tgctacgaat catttttgat ttcgcggaaa ggtgttgtca ctccatcttt ttggtgttag 14580 agtccgtctc aggtgctgaa cacaccttgt caggcggaag cgtcccgaaa gtcaaagcgc 14640 tttttcacgt ccgtaagttt tgcgcttgcc cccgatgccg ggacaatgca cgcttatggc 14700 cgggtagcgc gcagctatac aagacccttc gggggaaatc tgcgggcggt cctgacaccg 14760 tgttcaagta cccggcccgt cctctctgaa caagaggcga atttgaacga atgtcaggag 14820 gccaacatgg ctgctcaatc caatctgctc ccaaagggtg tcgctactaa cgacaccctt 14880 catcaaacct tcaacttcga gaacctgccg gttcgggcaa caaaccgaaa cggagaaatc 14940 tggttcgtag ccgctgacgt gtgtcgcgcg ctggagatca ccaacccccg catgctgtca 15000 tcgccttgac gatgacgaaa agggtgtcat cactgtggac acccttggcg gcccccagga 15060 gacggtcatc atcagcgaat cgggtctcta cagcctgacg ctgaccagtc gcaaagaaga 15120 ggccaagcgg ttcaagcggt ggattaccca cgaggttctt ccagccatcc gccaagacgg 15180 ggggttacca gaagcacgaa accacgcccg ccttggatgt gacccacgcc ccgactatga 15240 aggttgcgcg cgaggtagcc gacgactacc ttaatgccta ctatgaggcg atcaaggcgg 15300 gtaaaacacg ccatcaaatt gacggcgtac agcccgaagt gctccaagcc atcctggcgc 15360 atgcgctgat gagtcagcgg atgttggttt ccttcaattc catcggccaa atgaactgcc 15420 gcctgattcc cggccgatgc gtctgttgtg agctttgcca aggacgacta cttcaatctt 15480 gcccagcacg tcccgatgga gcgactgccc gacatgctgg atgcgctgaa caagcgagta 15540 gcttgccatc ttggcgcttt cagcgagcgc ttgcgccgcg actcttcgag gaatctgacg 15600 tgagtgatct gaccatcccg caaacgctat tgcacccgct caaggctaag gtggtggggt 15660 ttcatgagga agagaacgaa tacagcttcc aggtggaata ccccgatccc acggtctgcc 15720 cgaactgcgg ggtgtgcggc gaggtggtca ggttcggcaa gaagcaggtg aagttccgtg 15780 acctgccgct gcatgggcgg tgggtcacgc tctggctcat ccgccgacgc ttcgtgtgtc 15840 gggcgtgcaa cacgacattc agccccgcct tgcctgaaat ggctgagaac aaccgcatga 15900 cacagcggct ggccgagtac atcatcaagg ccgccgtggg gcgcaccaac agcgacgtgg 15960 gccgcgaggt gggcgtgaat gagtcgatca tccgcgccct gttccagagc cactacgagg 16020 cacagaaaca ccgctacacg ccatctgcgc cccgtgtgct gggcattgat gagctgtacc 16080 tgcaacgcac ctaccgctgc gtgctgacca atatcgagga ggaaaccatc atcgacctgt 16140 tggagtcgcg cacgaagcca caggtcacgg cctacctatc tcgtctcaaa gaccgaaagg 16200 acattgagat cgtctgcatg gatatgtgga acccgtaccg cgaggtggcc aaggcgctga 16260 tgccgcaagc cgccattgtg gtggacaagt tccacatcca gcgcatggcc aacgaggtca 16320 tggagaagat cagaagaggc atcaaggctg ggctgtcgca acatcgccgc cgtgcgctca 16380 aaggcgaccg caagctgatg ctgatgcgag agcgcgactt gacgcccatg agccaaatgg 16440 tggtggagac ctggacgaac cagttccccg agctgggcgc ggcctaccgc ctcaaggaag 16500 agttcatgaa catctggctg ctgaccgacg aagtggacgc cagagaggaa taccagcgct 16560 ggcaatccag cctgacaccg gagcagcggc cccaatgggc accgcttact accgcaatga 16620 gcaactggga ggcggaaatc ttcgcctact tcggtgacgg gcaacgccag accaatgcct 16680 tcaccgagtc gatcaaccgg caaatgaaag acctgaacag ggtcacgcgg acgatgtcct 16740 tcgagatgtt ccgagccaag atgctgttct cagtcgatca caagatgaag cgcaaggaaa 16800 agccgcgccg ggaatcgccc tttgaaatcg gctacggcgt ccctacggaa gccgaccttg 16860 caaggctggc acccaccatg ctcgacatgg gggtgcccat cggcaagctg ctggaacgct 16920 tcttggagcc gcccatcaac atcaccccac caccttcgga agccgggtag aaactccctt 16980 catcaaccgg cttttacgaa tacccgataa taatgatggg gctcatcgtg gacagcctgg 17040 tcatgatgag caaggtcgtt ttttacccgc ctggttaaaa actgcgtcag ggcctatcct 17100 gatatcaatg actgacatgg gcaatccaga ggtggctgct gaagggtttc gcaagggtga 17160 gtattattta tgccctaaag aaaagagaac gctatgcgtt cttgatccca gaagctatgg 17220 cacagacaag ggtcagcttt tattaccagt tttctctagc ccgataattg ttgccggaaa 17280 ttttgtcggc attgcgacca atgctccttc gattgccttt atacaaggtc tagctgaaac 17340 atctaacgag aagatgatgt cgggcgtcgg tgaagtggca attattggga agatggaaga 17400 gtcatcgcct acagtaagac aatagcctca tcactcacag cgtgaatgag atcctcaaca 17460 gcgacgaggt ggaactatac gaacgctcct tcgttcagct tacaaactgg atagagccct 17520 tggtgtcgtt gagctgttca tgccgttcgc ggttgctggt agtgaaacca actgacgatc 17580 atgatcagct tccgttggaa gccgtcgagt cggagttact gcatctacaa aaggatcatc 17640 cgtcaaagta gtgataactc tatactctct ctgggaattt ttggggggct tcttggaacg 17700 cttggaatag ttacaatgtt gtggctcagc gtaaggatcg ttacacctat acgagacctt 17760 cgacaaatgc tttatgaagt agctgccggt gatggtgatt tgacgggcag gttgaatatc 17820 aagaggaacg atgaagtggg tggtgtcgct accaatctta acggttttct tgataagtta 17880 cagcatatgg ttaaacagat catcatgtct gttcgccagg tccgactttc ctcaattgca 17940 tcgaccggca acgctgagca aacgaaaaat gggcttaact cccagaggga agaaatttca 18000 cttgttgtta ccgccattaa cgaaatggcc gcaactacgc aagaaatcgc ccgcagcgcg 18060 agtgaggcgt ctgaatctgc aagctatgcg aatgaacagg ccggcgtagg agctcaagtt 18120 gtctcggtca attccaatgc agttagatca ttggccgaag aaatttccaa tgctgcagag 18180 gtagtgagaa agctggcgtc agagagtgat aatataggcc agattttgaa ggttattcgc 18240 ggcattgccg aacagactaa tttgctagag 18270 // |
|