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EBI DbfetchID AF144563; SV 1; linear; genomic DNA; STD; PRO; 5971 BP. XX AC AF144563; XX DT 28-JUN-1999 (Rel. 60, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Thermobifida fusca beta-1,4-exocellulase E6 precursor (celF) gene, complete DE cds; and unknown genes. XX KW . XX OS Thermobifida fusca OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptosporangineae; Nocardiopsaceae; Thermobifida. XX RN [1] RP 1-5971 RA Irwin D.I., Zhang S., Wilson D.B.; RT "Characterization of a Thermomonospora fusca family 48 exocellulase E6"; RL Unpublished. XX RN [2] RP 1-5971 RA Irwin D.I., Zhang S., Wilson D.B.; RT ; RL Submitted (20-APR-1999) to the EMBL/GenBank/DDBJ databases. RL Biochemistry, Cornell University, 460 Biotechnology, Ithaca, NY 14853, USA XX DR StrainInfo; 501250; YX. XX FH Key Location/Qualifiers FH FT source 1..5971 FT /organism="Thermobifida fusca" FT /strain="YX" FT /mol_type="genomic DNA" FT /clone="pE6-91" FT /db_xref="taxon:2021" FT protein_bind join(540..551,633..646) FT /bound_moiety="CelR transcriptional regulator" FT /note="CelR transcriptional regulator encoded by GenBank FT Accession Number AF086819" FT RBS 880..884 FT /gene="celF" FT CDS 897..3851 FT /codon_start=1 FT /transl_table=11 FT /gene="celF" FT /product="beta-1,4-exocellulase E6 precursor" FT /note="family 48 exocellulase" FT /db_xref="GOA:Q9XCD4" FT /db_xref="HSSP:1FAE" FT /db_xref="InterPro:IPR000556" FT /db_xref="InterPro:IPR000601" FT /db_xref="InterPro:IPR001919" FT /db_xref="InterPro:IPR008928" FT /db_xref="InterPro:IPR008965" FT /db_xref="InterPro:IPR012291" FT /db_xref="InterPro:IPR012341" FT /db_xref="UniProtKB/TrEMBL:Q9XCD4" FT /protein_id="AAD39947.1" FT /translation="MRSLLSPRRWRTLASGALAAALAAAVLSPGVAHAAVACSVDYDDS FT NDWGSGFVAEVKVTNEGSDPIQNWQVGWTFPGNQQITNGWNGVFSQSGANVTVRYPDWN FT PNIAPGATISFGFQGTYSGSNDAPTSFTVNGVTCSGSQPANLPPDVTLTSPANNSTFLV FT NDPIELTAVASDPDGSIDRVEFAADNTVIGIDTTSPYSFTWTDAAAGSYSVTAIAYDDQ FT GARTVSAPIAIRVLDRAAVIASPPTVRVPQGGTADFEVRLSNQPSGNVTVTVARTSGSS FT DLTVSSGSQLQFTSSNWNQPQKVTIASADNGGNLAEAVFTVSAPGHDSAEVTVREIDPN FT TSSYDQAFLEQYEKIKDPASGYFREFNGLLVPYHSVETMIVEAPDHGHQTTSEAFSYYL FT WLEAYYGRVTGDWKPLHDAWESMETFIIPGTKDQPTNSAYNPNSPATYIPEQPNADGYP FT SPLMNNVPVGQDPLAQELSSTYGTNEIYGMHWLLDVDNVYGFGFCGDGTDDAPAYINTY FT QRGARESVWETIPHPSCDDFTHGGPNGYLDLFTDDQNYAKQWRYTNAPDADARAVQVMF FT WAHEWAKEQGKENEIAGLMDKASKMGDYLRYAMFDKYFKKIGNCVGATSCPGGQGKDSA FT HYLLSWYYSWGGSLDTSSAWAWRIGSSSSHQGYQNVLAAYALSQVPELQPDSPTGVQDW FT ATSFDRQLEFLQWLQSAEGGIAGGATNSWKGSYDTPPTGLSQFYGMYYDWQPVWNDPPS FT NNWFGFQVWNMERVAQLYYVTGDARAEAILDKWVPWAIQHTDVDADNGGQNFQVPSDLE FT WSGQPDTWTGTYTGNPNLHVQVVSYSQDVGVTAALAKTLMYYAKRSGDTTALATAEGLL FT DALLAHRDSIGIATPEQPSWDRLDDPWDGSEGLYVPPGWSGTMPNGDRIEPGATFLSIR FT SFYKNDPLWPQVEAHLNDPQNVPAPIVERHRFWAQVEIATAFAAHDELFGAGAP" FT sig_peptide 897..998 FT /gene="celF" FT mat_peptide 999..3848 FT /gene="celF" FT /product="beta-1,4-exocellulase E6" FT misc_feature 999..1313 FT /gene="celF" FT /note="Region: family II cellulose binding domain" FT misc_feature 1338..1604 FT /gene="celF" FT /note="Region: FNIII-like domain" FT misc_feature 1923..3848 FT /gene="celF" FT /note="catalytic domain; Region: family 48 glycosyl FT hydrolase" FT terminator 3931..3968 FT /gene="celF" FT /note="putative" FT CDS 4044..4430 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="Orf 2" FT /db_xref="UniProtKB/TrEMBL:Q9XCD3" FT /protein_id="AAD39948.1" FT /translation="MPLEPRLDIGADRTHPQPAGPHIIQRLPHQPAAHPLPLERAVHPS FT VEEDHPITGNRLVFRETGDFLPGTDLVPVLCRIVDHRDIHGVPLPFPRRRRISHPAACP FT GLSSSPGQPEHAPARRTPATALAW" FT CDS 4473..5480 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="Orf 3; similar to SuA5 superfamily related proteins" FT /db_xref="HSSP:1JCU" FT /db_xref="InterPro:IPR004388" FT /db_xref="InterPro:IPR005145" FT /db_xref="InterPro:IPR006070" FT /db_xref="InterPro:IPR010923" FT /db_xref="InterPro:IPR017945" FT /db_xref="UniProtKB/TrEMBL:Q9XCD2" FT /protein_id="AAD39949.1" FT /translation="MGDGGTVRHPSVRAVSPAALDEAVRVLRAGGLVAFPTETVYGLGA FT DAANPAAVARIFAAKGRPADHPLIVHVASAESARDWAATFPPLARTLADAFWPGPLTLI FT LPRSAQVPDAVTGGRATVGLRVPDQPVALALLERFGGGIAAPSANRFGRVSPTTAAHVA FT ADLGDRVDLVLDGGPCTVGVESTIVEVADGRLTVLRTGGITPDDLAAVTGAPVDTTPTG FT PARAPGMLAAHYAPAARVVLAEAAEAADTVAQWVEKGHRVAVLAETATVPENLPEGVVV FT LPSPASARDYARVLYQRLRDVDAAGADVVVAIPPEPAGIGLAVRDRLLRASRAH" FT CDS 5654..>5971 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="Orf 4" FT /db_xref="InterPro:IPR015035" FT /db_xref="InterPro:IPR015057" FT /db_xref="UniProtKB/TrEMBL:Q9XCD1" FT /protein_id="AAD39950.1" FT /translation="MLKVQGDNGGPPYWVRWEDEGRETLVYPGPDARIEPRHPVPQARQ FT EHTEARQPQPGQSLKRIQIDVAVSEVHENGSVRTLAEAQLPSTKWNLRGHGEARKHPTD FT AD" XX SQ Sequence 5971 BP; 1023 A; 2244 C; 1836 G; 868 T; 0 other; gcggccgcca ggtggggcgg ccgagtccga cggtggcgag gacttcgacc ccgtggtcgt 60 cagcgaagca tgcttcgctg acgttggcgg cggtgagcag gcctaccccg gtgccggtca 120 tcccgaagtg ggtggcgagt tcttcgaggt ggttgtccgg gtcggtccgc cggtagtcgc 180 cgtcgacttg ggcgttgatg acgtagtccc gtggtccgag gccgccgccg agcacgctgg 240 aggcggccat gcgccagccg gggccgcact gccagagcac tgcggcaagg gcgcggccgt 300 tgtcgcgccg ccagcgcagg tgcgcggtga ggagggtgcc gtcgccgatc gtctccgtgt 360 ccactccgct ctccagggtc ggttccgcgg ggccacttta acgagtccgc atcgggcggc 420 agcggggtca tgcttccatc tgggcgaatg ccacgcggag tactgcgagt ctcacgcttc 480 gtcccattgt ctttttacgg agggtttaca cggcacatcc ggaacgttac cctcctactg 540 ggagcgctcc cgtgactcag gccacaggcc cccttcccgg ggcgagcccc caccaccccg 600 ggaaggccac tgcgacccct gagctcaatt catgggagcg ctcccatgcc agtgctcccg 660 gcagaaagga gagaaccgga acccggtacc gctggtttca ctgatccccc tgttttcacc 720 cggcatcgcc ccgacaccag cgatgccacc cccgcggcgg ctgcagtcca cacccggcac 780 gccgcacacc cctctcctgt gtgacacgcg gttccgaacg gccttgccgg ttctcggtat 840 gccgacgaac ggcacggctg ctccggaacc ggaagatccg gaggttattt ccaagcatga 900 gatcgttact gtctccccgg cgctggcgca cgctggcctc gggggcgctc gcagcggccc 960 tggccgccgc tgtactctcc cccggcgtcg cgcacgccgc cgtcgcctgc tcggtggact 1020 acgacgactc caacgactgg ggtagcgggt tcgtcgccga agtcaaggtg accaacgaag 1080 gcagcgaccc catccagaac tggcaagtag gctggacctt ccccggtaac cagcagatca 1140 ccaacggctg gaacggcgtg ttcagccaga gcggcgccaa cgtcaccgtc cgctacccgg 1200 actggaaccc caatatcgcc cccggagcca ccatctcctt cggcttccag ggcacctaca 1260 gcggctccaa cgacgccccg accagcttca ccgtcaacgg cgtcacctgc agcggatccc 1320 agcccgccaa cctgccgccc gatgtcaccc tgacatcccc ggccaacaac tcgaccttcc 1380 tggtcaacga cccgatcgag ctgaccgcgg tcgcctccga ccccgacggc tcgatcgacc 1440 gggtggaatt cgccgccgac aacaccgtca tcggcatcga caccacctcc ccctacagct 1500 tcacctggac ggacgctgcc gccggctcct actcggtgac cgcgatcgcc tacgacgacc 1560 agggagccag gaccgtctcc gctcccatcg ccatccgagt gctggaccgg gccgccgtca 1620 tcgcctcacc gcccaccgtc cgcgtgccgc agggcggcac cgccgacttc gaggtgcggc 1680 tgtccaacca gccctccggc aacgtcacgg tcaccgtggc gcgcacgtcg ggcagctccg 1740 acctgaccgt ctccagcggc tcccaactcc agttcacctc cagcaactgg aaccagccgc 1800 agaaggtgac catcgcctcc gctgacaacg gcggaaacct ggccgaggcg gtcttcaccg 1860 tcagcgcccc cggccacgac tcggccgagg tgacggtccg ggagatcgac ccgaacacca 1920 gctcctacga ccaggccttc ctggagcagt acgagaagat caaggacccc gccagcggct 1980 acttccgcga attcaacggg ctcctggtcc cctaccactc ggtggagacc atgatcgtcg 2040 aggctccgga ccacggccac cagaccacgt ccgaggcgtt cagctactac ctgtggctgg 2100 aggcgtacta cggccgggtc accggtgact ggaagccgct ccacgacgcc tgggagtcga 2160 tggagacctt catcatcccc ggcaccaagg accagccgac caactccgcc tacaacccga 2220 actccccggc gacctacatc cccgagcagc ccaacgctga cggctacccg tcgcctctca 2280 tgaacaacgt cccggtgggt caagacccgc tcgcccagga gctgagctcc acctacggga 2340 ccaacgagat ctacggcatg cactggctgc tcgacgtgga caacgtctac ggcttcgggt 2400 tctgcggcga cggcaccgac gacgcccccg cctacatcaa cacctaccag cgtggtgcgc 2460 gcgagtcggt gtgggagacc attccgcacc cgtcctgcga cgacttcacg cacggcggcc 2520 ccaacggcta cctggacctg ttcaccgacg accagaacta cgccaagcag tggcgctaca 2580 ccaacgcccc cgacgctgac gcgcgggccg tccaggtgat gttctgggcg cacgaatggg 2640 ccaaggagca gggcaaggag aacgagatcg cgggcctgat ggacaaggcg tccaagatgg 2700 gcgactacct ccggtacgcg atgttcgaca agtacttcaa gaagatcggc aactgcgtcg 2760 gcgccacctc ctgcccgggt ggccaaggca aggacagcgc gcactacctg ctgtcctggt 2820 actactcctg gggcggctcg ctcgacacct cctctgcgtg ggcgtggcgt atcggctcca 2880 gctcctcgca ccagggctac cagaacgtgc tcgctgccta cgcgctctcg caggtgcccg 2940 aactgcagcc tgactccccg accggtgtcc aggactgggc caccagcttc gaccgccagt 3000 tggagttcct ccagtggctg cagtccgctg aaggtggtat cgccggtggc gccaccaaca 3060 gctggaaggg aagctacgac accccgccga ccggcctgtc gcagttctac ggcatgtact 3120 acgactggca gccggtctgg aacgacccgc cgtccaacaa ctggttcggc ttccaggtct 3180 ggaacatgga gcgcgtcgcc cagctctact acgtgaccgg cgacgcccgg gccgaggcca 3240 tcctcgacaa gtgggtgccg tgggccatcc agcacaccga cgtggacgcc gacaacggcg 3300 gccagaactt ccaggtcccc tccgacctgg agtggtcggg ccagcctgac acctggaccg 3360 gcacctacac cggcaacccg aacctgcacg tccaggtcgt ctcctacagc caggacgtcg 3420 gtgtgaccgc cgctctggcc aagaccctga tgtactacgc gaagcgttcg ggcgacacca 3480 ccgccctcgc caccgcggag ggtctgctgg acgccctgct ggcccaccgg gacagcatcg 3540 gtatcgccac ccccgagcag ccgagctggg accgtctgga cgacccgtgg gacggctccg 3600 agggcctgta cgtgccgccg ggctggtcgg gcaccatgcc caacggtgac cgcatcgagc 3660 cgggcgcgac cttcctgtcc atccgctcgt tctacaagaa cgacccgctg tggccgcagg 3720 tcgaggcaca cctgaacgac ccgcagaacg tcccggcgcc gatcgtggag cgccaccgct 3780 tctgggctca ggtggaaatc gcgaccgcgt tcgcagccca cgacgaactg ttcggggccg 3840 gagctccctg atcctgaaca gtccgtctcc tgaccggcgg tccgtggcgg cgtagtgctc 3900 ccccgccgcg gaccgccctc caccaccccc ctggggccgt ccggcgcacc aacaccggac 3960 ggccccagac tcctttcacg gcctgctact cggcccgacg ccccgcccta tccagcccga 4020 tcaaccaggt cagcgtagtc cgggtgccgc tcgagccacg ccttgacata ggggcagacc 4080 gcacgcaccc gcagcccgcg ggcccgcaca tcatccaacg cctcccgcac cagccggctg 4140 cccatcccct tcccctggaa agagccgtcc acccgagtgt ggaagaagac cacccgatca 4200 ccggcaacag gctggtattc cgtgaaaccg gcgattttct ccccggcacg gatctcgtac 4260 cggtgctgtg ccggattgtc gatcaccgtg acatccatgg cgttcccctc ccgttcccca 4320 ggcggcggcg catctctcat ccggcggcct gcccaggact gtcctcttcc ccaggacagc 4380 ctgagcacgc gcccgcacgg cgaaccccag ccacagccct agcatggtga acggccacgg 4440 tcgcagggcg accacgacac cgaggaggag tgatgggcga cggcggcaca gtacgccacc 4500 cttccgtacg tgccgtatca ccggcagcgt tggacgaggc ggtacgcgtc ctgcgggcag 4560 gcggcctcgt agctttcccc accgaaaccg tctacggcct aggcgcggac gccgcgaacc 4620 cggccgcggt agcgcgcatt ttcgccgcca aaggccgtcc cgctgaccac cccctgatcg 4680 tgcacgtcgc ttccgcggag agcgcgcgcg actgggctgc tacctttcct cctctcgccc 4740 gcacgctggc ggacgcgttc tggcccggcc cgctgaccct catcctcccc cgctccgccc 4800 aagtgccgga cgcagtgacc ggtggacgcg ccacggtagg gctgcgggtc cccgaccagc 4860 cggtcgcgct cgcactcctg gaacgcttcg gcggcggaat cgccgcacct tccgcgaacc 4920 ggttcggccg agtgagcccg accacggccg cgcacgttgc cgctgacctc ggggaccggg 4980 tcgacctggt gttggacggc ggaccgtgca cggtcggcgt ggaatcgacg atcgtcgaag 5040 tggccgacgg ccggctcacc gtgctccgca ccggaggcat cacccccgac gaccttgccg 5100 cggtcaccgg agcccccgtc gacaccaccc ccaccggacc ggcccgggca cccggcatgc 5160 tcgccgccca ctacgcaccc gccgcacggg tcgtgctagc cgaagcagca gaagccgcgg 5220 acacggtcgc ccagtgggtg gagaaaggac accgggtggc tgtgctggcg gagaccgcta 5280 ccgtgcccga aaacctgccg gaaggcgtgg tggtgctacc gtcccctgct tcggctcggg 5340 actatgcccg cgtgctgtac cagcggctgc gggacgtgga cgcggcggga gccgacgtgg 5400 tcgtcgcgat cccccccgaa cccgcgggga tcggcttggc ggtacgggac cggctgctgc 5460 gcgcatcccg ggcgcactga cctctccccc tggggcaagg gatttttccg catagacgag 5520 cccgtttccg ggatctcttt ctgtggagac agaaagagag caccgacacc agggagggcc 5580 gatgaaagct caagccggag accggatcgt tgtggaacgc ccccgcgatg acctgcccgc 5640 gcgcaaaggc gtcgtgctca aagtgcaggg ggacaacggg ggcccgccct actgggtgcg 5700 gtgggaggac gaaggccggg aaaccctcgt ctacccggga ccggacgccc gtatcgagcc 5760 ccgccacccc gtcccccaag cccgccagga gcatacggag gcccgtcaac cgcagccggg 5820 acagtccctg aaacggatac agatcgacgt cgcagtgtcg gaggtgcacg agaacggctc 5880 cgtgcgcacc ctcgccgaag cgcaactgcc gtccaccaag tggaacctgc gcggccacgg 5940 agaagcacgc aagcatccca ccgacgccga t 5971 // |
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