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EBI DbfetchID AF135191; SV 1; linear; genomic DNA; STD; PRO; 5086 BP. XX AC AF135191; XX DT 15-FEB-2000 (Rel. 62, Created) DT 15-FEB-2000 (Rel. 62, Last updated, Version 1) XX DE Bacillus sp. NEB-606 RadC (radC) gene, partial cds; and BslIM (bslIM), DE BslIRalpha (bslIRalpha), and BslIRbeta (bslIRbeta) genes, complete cds. XX KW . XX OS Bacillus sp. NEB-606 OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-5086 RX DOI; 10.1128/JB.182.4.949-955.2000 RX PUBMED; 10648519. RA Hsieh P.C., Xiao J.P., O'loane D., Xu S.Y.; RT "Cloning, expression, and purification of a thermostable nonhomodimeric RT restriction enzyme, BslI"; RL J. Bacteriol. 182(4):949-955(2000). XX RN [2] RP 1-5086 RA Xiao J.P., Hsieh P.C., Xu S.Y.; RT ; RL Submitted (16-MAR-1999) to the EMBL/GenBank/DDBJ databases. RL Research, New England Biolabs, Inc., 32 Tozer Road, Beverly, MA 01915, USA XX FH Key Location/Qualifiers FH FT source 1..5086 FT /organism="Bacillus sp. NEB-606" FT /strain="New England Biolab #606" FT /mol_type="genomic DNA" FT /db_xref="taxon:114630" FT CDS <1..360 FT /codon_start=1 FT /transl_table=11 FT /gene="radC" FT /product="RadC" FT /note="putative DNA repair enzyme" FT /db_xref="GOA:Q9LAI3" FT /db_xref="InterPro:IPR001405" FT /db_xref="UniProtKB/TrEMBL:Q9LAI3" FT /protein_id="AAF32528.1" FT /translation="PEDAYNLVKCFLVDVDRDYFLVICLDTKNQPTAINICHVGSLNAS FT LVHPREVMKPAILSNSASVIVAHNHPSLDPTPSKEDINVTKRLIEAGRIMGIDILDHIV FT VGGNKYVSLKEKRYI" FT CDS 515..3253 FT /codon_start=1 FT /transl_table=11 FT /gene="bslIM" FT /product="BslIM" FT /function="modifies 5' ccn7gg 3' sequence" FT /note="BslI methylase; N4 cytosine methylase" FT /db_xref="GOA:Q9LAI2" FT /db_xref="InterPro:IPR001091" FT /db_xref="InterPro:IPR002941" FT /db_xref="InterPro:IPR017985" FT /db_xref="UniProtKB/Swiss-Prot:Q9LAI2" FT /protein_id="AAF32529.1" FT /translation="MNWIFNTLIQFLEDLNIDPSVVSLIDEDAKKLEEQFPKALKHPVV FT DEEIVYKILCEKYNLNALNVKTISETLNKEYKFGRNSKTALKKYLDYGKEEYLIQFFNT FT LMLENNTYIDREYIESVLAFCEPVSKEKIKNEFIKLWNEANEVNEYGKLKDYLLGIYSK FT LFSMGLENLRLIEIYNSNESLIKKVFKYESTIKELKEYCLSNQESITAGLAIKMFNEKY FT MELMKKEYQQDAIALKLEEHMNQLYVDNNINEYPYIFDRGNDILLLPTEEYDFVYFHID FT QDFFNRFQDENKFLDYVLSSIKQIYRVLANEKVFALKIDNIYNNEKNLKWELYPKLTIY FT SEHFIQTKETARFYKAYDIAKDLLSKHEFRLLENDSEKNRENILKEYFSGKISEDELFS FT LVHVNMKKEHFFEFLNRFKYVHYGFTFNDCLVLDRVDKSFANGELENVISNATEILLIF FT YKFRADQRRIPCPSCGSLNISGNSYPEINNRSWECKSPYCPDRSKSNRGKRYSKKSNYM FT QWGAIYPKSHDIIPRELIKKWRRDIIVINNEQEIFEMLVKYFSFTDEKLLFINTNELPS FT VVTERENRKVVILSQKLKEKAYTSNVVVKESLEGEIEFFKNGLYLKNFTELYLPEDQRR FT VSPEINNFLNSGGRLKLIQGDSYEVLKSVEDNTFAAAVTSPPYYNAREYSQWPNLYLYF FT NDMYNIIKECFRTLKPGSVFLYNIADIVDNENIIVKSSMGNKRIPLGAYTIYFFQKAGF FT ELLDNIIWDKGEPQSNRQKNDGKFTPHYQKPLNAYEHMFIFKKTGAPLTLSDDWQSKRG FT SWIKNIVPFQPVFKINSKGENILGHTAPFPEDIPRFVANVFTKHDNDIILDPFSGSLTS FT AIASYKSNRIGLGIELSPDYVELSRDRALLEGVTTKILNFN" FT CDS complement(3299..3976) FT /codon_start=1 FT /transl_table=11 FT /gene="bslIRalpha" FT /product="BslIRalpha" FT /note="BslI endonuclease alpha subunit" FT /db_xref="GOA:Q9LAI1" FT /db_xref="UniProtKB/Swiss-Prot:Q9LAI1" FT /protein_id="AAF32530.1" FT /translation="MERQLKSIAYAFVANDIDVYIPDGESNCIVVTKLVCKDCGQYWHT FT SLSECYFCGTLNFYLYECNSCGKKYSLTSSSKSCDTDGCNGKLIKRCSNPECISRTNEE FT IQRATDEQGGVFDLNSSFNVSLNHCVTCGSKENYYKTYRIYSYRTEVEPNIEALREFAN FT NNKLNSDEDVIIIKHLVDNVIHYGYIPYSKLDETTEITTTFSRFSDLVSELFPVNVPPN FT VTE" FT CDS complement(3976..4881) FT /codon_start=1 FT /transl_table=11 FT /gene="bslIRbeta" FT /product="BslIRbeta" FT /function="cleaves 5'ccnnnnn^nngg3' sequence" FT /note="BslI endonuclease beta subunit" FT /db_xref="GOA:Q9LAI0" FT /db_xref="UniProtKB/Swiss-Prot:Q9LAI0" FT /protein_id="AAF32531.1" FT /translation="MEQQKFPNPRIFEDIDATDFSKHNKKHVTEDFVAENFKDVGWRVY FT RPFNDTGIDLIAKKFVCPDGHTKWNQNLTKEMTCSECGKSLIEITRFIQVKTREVKQVK FT TREAKGEKFFFGYTLKSKDFRTDPRHVFLLYSDFTMDFIILPMYDYLNLFYTNQSLGST FT HFSTPSFRQGNNKLNGLSKDKNDNWVWSGVSFNEFVNEKGMDKLSCPIYDIELESYTKK FT IQELKFSLFYRYSPGRKNQVSAPTVEFINNHFSIFISLPKEAIASKRKAHLESLRQDLP FT EDLKKSVNEGYLVKFKGVDL" XX SQ Sequence 5086 BP; 1827 A; 704 C; 838 G; 1717 T; 0 other; ccggaggatg cctacaattt agtgaagtgt ttcttagttg acgttgatcg ggactatttt 60 ctagtgattt gtttagatac aaaaaaccaa ccaacagcaa ttaatatttg tcatgtaggt 120 agtttgaatg caagtttggt tcaccctaga gaagtaatga aaccagctat actttctaat 180 tctgcttcag ttattgtagc acataatcat cctagtttag atccaacacc tagtaaagaa 240 gatattaatg taactaagcg tttaatagag gcagggagaa ttatggggat agatatattg 300 gaccatattg tagttggagg taataagtat gtatctctta aagagaaaag atatatttag 360 aattatttta ataatttaac agtgttttac caacaaaaat aagaaagtaa accatggtag 420 caactaacat ggatataatt tatggagtgg tatataatga tatgatgtga attatggagt 480 ggtatataaa taaaatatct ggagggccat tagaatgaat tggatattta atactctgat 540 tcaatttctt gaagatttaa atatagatcc gagcgtagta tctttgattg atgaggatgc 600 taaaaagctt gaagaacaat tccctaaggc attaaaacat ccagttgtag atgaggaaat 660 tgtatacaaa atactttgtg aaaagtataa tctaaatgct ttaaatgtaa aaacaatatc 720 tgagacttta aataaagaat ataaatttgg aaggaattcg aaaactgcgt taaaaaagta 780 tcttgattat ggtaaagagg agtatttgat tcaatttttt aataccctta tgctagaaaa 840 caatacatat atagatagag agtatattga aagtgtgctg gctttttgtg aacctgtttc 900 aaaagaaaaa attaaaaatg agtttataaa gctttggaat gaagctaatg aagttaatga 960 atacggtaag ttaaaggatt acttattggg aatttattca aagctattct caatgggact 1020 agaaaattta agactaatag aaatttataa ttctaatgaa agccttataa aaaaggtatt 1080 taaatacgag tcaacgataa aggagttaaa ggagtactgc ttatctaatc aagagtcaat 1140 tactgctggt ttagccatca agatgtttaa tgaaaagtat atggaattaa tgaaaaaaga 1200 atatcaacaa gatgctatag ccttaaaact tgaggagcat atgaatcaat tgtatgttga 1260 taataatatt aatgaatatc cttatatttt tgaccgggga aatgatattc tactcttacc 1320 tacagaagag tatgactttg tttatttcca tatagatcag gattttttta atagattcca 1380 agatgaaaat aaattcttgg attatgtact ttcgtccata aaacaaattt atcgtgtgtt 1440 agctaatgaa aaagtttttg cgttgaagat tgataatatt tacaataatg aaaaaaattt 1500 gaaatgggaa ctttatccaa aactaacaat ctactctgaa cattttatac aaacaaaaga 1560 aactgctagg ttttataaag catacgatat agctaaagat ttgcttagta aacacgaatt 1620 taggctatta gagaatgatt cagagaaaaa tagagaaaat attttaaaag agtatttttc 1680 tggaaaaata agtgaagatg agttattttc tttagttcat gtaaatatga aaaaagaaca 1740 tttctttgaa tttctaaaca gatttaaata tgtacattat ggttttacat ttaatgattg 1800 tctagtgtta gacagggttg ataaaagctt tgcaaatggt gagctagaaa atgtcataag 1860 taatgcaaca gaaatacttc ttattttcta taagtttaga gcggatcaaa ggagaattcc 1920 ttgtccttct tgtggtagtt tgaatatttc tgggaactct tacccagaaa taaataatag 1980 aagctgggaa tgtaaatctc cttattgtcc agacaggagt aaatctaatc gtggtaaacg 2040 atattctaaa aaatctaatt atatgcaatg gggagctatt tatccaaaat ctcatgacat 2100 cattcctcga gaattaatta aaaagtggag aagagatata attgtaatta ataatgaaca 2160 agaaatcttt gagatgcttg tgaaatactt tagtttcaca gatgaaaaat tgttatttat 2220 caatacgaat gaactaccta gtgtagttac agaacgtgaa aatagaaagg ttgttatatt 2280 atctcaaaag ctgaaagaaa aagcatatac aagtaatgta gttgtaaagg aaagcttaga 2340 aggagaaata gagtttttca agaacggttt atatctcaag aattttactg agttgtattt 2400 accagaggat caaagaagag tctctcctga aataaataac tttttaaata gtgggggacg 2460 gttaaaatta atacaaggag atagttacga agtattaaaa agtgtagaag ataatacttt 2520 tgcagcagca gtgacttcgc ctccatacta caatgctagg gaatattctc aatggccgaa 2580 cctatattta tactttaatg atatgtataa cattattaaa gaatgcttta gaactctaaa 2640 accgggtagt gtattccttt ataacattgc tgatatcgtt gacaatgaaa atataatagt 2700 caaatcatca atgggaaata aaagaatccc tcttggtgca tatactattt atttcttcca 2760 aaaggcaggt tttgagctac tagataatat tatatgggat aaaggcgagc cacaaagtaa 2820 taggcaaaaa aatgatggta agtttacacc tcactatcaa aagccactaa atgcttatga 2880 gcatatgttt atttttaaaa agacaggcgc tcctttaact ttaagtgacg attggcaaag 2940 taaacgagga agctggatta aaaatatagt accttttcag cctgttttta agataaattc 3000 aaaaggtgaa aatatcttag gtcacacagc accattccct gaagacattc caagatttgt 3060 tgctaatgtg tttacaaaac atgataatga tataatatta gatccatttt caggctcatt 3120 aacttcagct atagcttcgt ataaaagcaa tagaataggt cttggaattg agttgtctcc 3180 tgattatgtt gaattatcta gggacagagc gttattagaa ggggtaacta ctaaaatttt 3240 aaattttaat taatgtataa aataaaaaag atagctttca caaagaaagc tatctttttt 3300 attcagtaac atttggagga acgttaactg ggaacaattc tgaaacaaga tcagaaaaac 3360 gagaaaatgt tgtagtaatt tctgttgttt catctaactt actgtatgga atataaccgt 3420 aatgaattac attatcaact aggtgtttaa tgattataac atcttcatca ctattaagtt 3480 tgttgttatt ggcaaactct cttaaagctt ctatatttgg ttctacttcc gttcgataag 3540 agtatatacg atatgttttg taatagtttt ctttacttcc acatgtcaca caatgattta 3600 aagatacgtt aaaagatgaa ttgagatcaa atactcctcc ttgctcatct gttgcacgct 3660 gtatttcttc atttgtccga ctaatacatt ctggattgct acatcttttg attaatttac 3720 cattacaacc atcagtatca caagattttg aagaagaagt taatgaatat ttttttccac 3780 aagaattaca ttcatataga taaaaattca aagtaccaca aaaataacac tctgataagc 3840 tagtatgcca atattgcccg caatccttgc aaaccaattt ggttaccact atacaatttg 3900 attctccatc tggtatatac acatctatat cgtttgcaac aaaagcgtaa gcaattgatt 3960 ttaattgacg ttccattata agtcaacccc tttaaatttg actaggtatc cttcgttaac 4020 actctttttc aaatcttctg ggagatcttg acgtaggctt tcaagatgtg ccttcctttt 4080 gctagcaata gcttcctttg gtagacttat aaaaatgcta aaatgattat taataaattc 4140 gacagtagga gctgatacct gattttttct accaggagag taccgataga ataagctaaa 4200 ttttaattct tgtattttct tagtatagct ctctaattca atatcgtaga ttggacagct 4260 aagtttatcc ataccttttt catttacgaa ttcattaaac gaaactccac tccaaaccca 4320 gttatcattc ttgtctttcg aaagaccgtt cagtttatta ttcccttggc gaaaagaagg 4380 ggtactaaag tgtgttgagc ctaaactttg gttagtataa aacaagttta gataatcata 4440 cataggaaga ataatgaaat ccatagtaaa atctgagtat aagagaaata catgtctagg 4500 atcggttcgg aaatcctttg attttaaggt gtaaccgaaa aaaaactttt ctcctttagc 4560 ttcccgagtt tttacttgtt taacttcccg agtttttact tgaataaaac gtgttatttc 4620 aattaatgat tttccgcatt cactacaagt catttctttt gttagatttt gattccactt 4680 tgtatgtcca tcaggacata caaatttctt ggcaataaga tcaattcctg tatcgttaaa 4740 aggacgataa actctccaac caacatcttt aaagttttca gccacaaagt cctctgttac 4800 atgcttttta ttatgttttg aaaaatcagt tgcatcaata tcctcaaaaa ttcttggatt 4860 agggaatttt tgttgttcca tcaataactc tcctttagtt ttgatttttg taaaattgtt 4920 taaggtaaac tcagttatta tatctcccat cagaataaat taacatgcgg ggaaataatt 4980 ctattatgta agttttgact aattttgttg aacagtgtct acactttcct tcctaatact 5040 atgatatact ctatattata tatcattagt acaacttcat aagctt 5086 // |
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