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EBI DbfetchID AF121273; SV 1; linear; genomic DNA; STD; PRO; 4651 BP. XX AC AF121273; XX DT 21-JAN-2000 (Rel. 62, Created) DT 14-JUL-2007 (Rel. 92, Last updated, Version 2) XX DE Alteromonas agarilytica alpha-agarase precursor (agaA) gene, complete cds. XX KW . XX OS Alteromonas agarilytica OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; OC Alteromonadaceae; Alteromonas. XX RN [1] RP 1-4651 RX DOI; 10.1128/AEM.00496-07 RX PUBMED; 17513582. RA Flament D., Barbeyron T., Jam M., Potin P., Czjzek M., Kloareg B., RA Michel G.; RT "Alpha-agarases define a new family of glycoside hydrolases, distinct from RT beta-agarase families"; RL Appl. Environ. Microbiol. 73(14):4691-4694(2007). XX RN [2] RP 1-4651 RA Flament D., Barbeyron T., Potin P., Kloareg B.; RT "The alpha-agarase from Alteromonas agarilytica strain GJ1B is a novel RT glycoside hydrolase which features type-VI cellulose-binding domain"; RL Unpublished. XX RN [3] RP 1-4651 RA Barbeyron T., Flament D.; RT ; RL Submitted (19-JAN-1999) to the EMBL/GenBank/DDBJ databases. RL Centre d'Etudes Oceanographiques et de Biologie Marine, CNRS, Place Georges RL Teissier, Roscoff, Finistere/Bretagne 29250, France XX DR StrainInfo; 373746; DSM 12513. XX FH Key Location/Qualifiers FH FT source 1..4651 FT /organism="Alteromonas agarilytica" FT /strain="GJ1B" FT /mol_type="genomic DNA" FT /note="DSM 12513" FT /db_xref="taxon:105692" FT gene 137..4529 FT /gene="agaA" FT -35_signal 137..142 FT /gene="agaA" FT /note="putative" FT -10_signal 159..164 FT /gene="agaA" FT /note="putative" FT RBS 180..183 FT /gene="agaA" FT /note="Shine-Dalgarno sequence" FT CDS 190..4479 FT /codon_start=1 FT /transl_table=11 FT /gene="agaA" FT /product="alpha-agarase precursor" FT /function="hydrolyzes alpha(1,3) linkage in agars or FT agarose" FT /note="unclassified glycoside hydrolase" FT /db_xref="GOA:Q9LAP7" FT /db_xref="InterPro:IPR003367" FT /db_xref="InterPro:IPR005084" FT /db_xref="InterPro:IPR006584" FT /db_xref="InterPro:IPR008979" FT /db_xref="UniProtKB/TrEMBL:Q9LAP7" FT /protein_id="AAF26838.1" FT /translation="MFKTKRSLLNSSIAISFAVLGVQAQAETLELQAESFANSGGTYSD FT GQPNPVTIYNVNGQGAINFVNAGDYVDYNINALGGEYDIEYFVGTGVTSGPNIEVLVDV FT NGTWQSQGSVAVPYGSWDDFQSLTPSHTVTLPVGTSTVRLLAVGSTWQWNLESFRLTQV FT SPVEPVGDADNDGVNDNQDLCPNSPSGVTVDNNGCQITGGTDPGGESFVIQMEAFDSTG FT SDDSRAKGVIIGERGYPQDKHTVVDSVQTTDWVDYSINFPSSANYSVSMLASGQTDHAT FT AVLYLDGTEINEVPVHTGSQADFANFQLAGSVYIASGTHTIRVQAQSSTGEFSWLWFGD FT ALTFTNLDSDGGNGGEATQDADNDGVLDSSDSCPNTPTGEPADVTGCSASQLDDDNDGV FT SNNVDQCPNTVAGTEVDADGCEVIFADADNDGIEDSQDFCPNTPAGEAVNNSGCGASQL FT DADNDGVTNNIDQCPNTPAGTQVDASGCETDNGGEPGDSYYHNGQGLLFGRVDGATNFL FT GEEGYVANPDNYDVTTDLLETDDAIRANSTEVFRGEIYDADGHIAFYEHIDDSVRLYID FT GQLVLSNDSWENSSQTTDLNLTPGWHNFELRLGNADGGSGAVSGIGFGIDVDGGTNFVH FT PSNLSPSMFRASGQVVVDPILPPSGGIYIQLEDFDETGTVGRVASDPNDGFVKGDSNVG FT WVTNGDWGKYHNVFLEAGTYRAFITVSTPAGGSYGARVDIDGEPFAWGYFDSTGGWDIA FT AEYELYGGHLVVESTGNHTLHVEAVGGSDWQWSGDLVRLAKVSDSAVKQPRVYNPNEHI FT VAEIQGPATGLQYLKTPVEIPLANKVLKSDVWYTYPQNRNLVVDGDTPYADFGATGAFW FT GHPPEHDFYDDTVIMDWAVNVVDDFQSEGFEYTARGEFDWGYGWFTEFTTNPQPHYVQT FT LDGRNVRMTFMGYLSHDGYNNNWLSNHSPAFVPFMKSQVDQILKANPDKLMFDTQTNST FT RSTDMRTFGGDFSPYAMENFRVWLLKKYSNAQLVSMGINDITSFDYGAYLRAQGITHTD FT WSNAGDTISGNIPMMEDFIYFNRDVWNQKFAEVLEYIRQQRPNIEIGASTHLFESRGYI FT FNENITFLSGELNLGARTSISELPTNILVHLKGAQAVDKTLAYFPYPWEFDELRIQNAP FT RFGRGWVAQAYAYGGLFSIPANVWVGGEVFTWSPGADNYRDIYQFVRAQANLLDGYTSY FT AKAGYVHAMFSSMKAGFIDGGNQVQSSVKILTEDNINFDMLVFGDAGYPVVPRQADFDK FT FEYIFYDGDLNYLTAEQQAVLDAQGSKVKHIGQRGTIAGLQINVSINGSVSNETVSAVS FT RIHETDSTAPYVVHLINRPFAGGVTPILNNVEVAIPASYFPQGVTSAKLHLPDGSSSTV FT AVSTNANGDTVVSVSNLEVWGILELAH" FT sig_peptide 190..267 FT /gene="agaA" FT mat_peptide 268..4476 FT /gene="agaA" FT /product="alpha agarase" FT misc_feature 268..2559 FT /gene="agaA" FT /note="Region: putative non-catalytic domain" FT misc_feature 280..669 FT /gene="agaA" FT /note="Region: putative type VI-cellulose binding domain A" FT misc_feature 700..798 FT /gene="agaA" FT /note="Region: putative type 3-calcium binding domain I" FT misc_feature 808..1215 FT /gene="agaA" FT /note="Region: putative type VI-cellulose binding domain B" FT misc_feature 1267..1365 FT /gene="agaA" FT /note="Region: putative type 3-calcium binding domain II" FT misc_feature 1366..1464 FT /gene="agaA" FT /note="Region: putative type 3-calcium binding domain III" FT misc_feature 1465..1563 FT /gene="agaA" FT /note="Region: putative type 3-calcium binding domain IV" FT misc_feature 1564..1659 FT /gene="agaA" FT /note="Region: putative type 3-calcium binding domain V" FT misc_feature 2170..2559 FT /gene="agaA" FT /note="Region: putative type VI-cellulose binding domain C" FT misc_feature 2560..4476 FT /gene="agaA" FT /note="Region: putative catalytic domain; no similarity FT with other glycoside hydrolases" FT terminator 4507..4529 FT /gene="agaA" FT /note="stem loop" XX SQ Sequence 4651 BP; 1307 A; 827 C; 1109 G; 1408 T; 0 other; ggatcgacag aacaggggtg atatttgttt tttttaatat ctccctgttt tttttgtttt 60 attgagtatt gtcatcaatt aacaaaaagt ctttgttgtg cgattgttta caagcagctg 120 tttgttcgaa acaaggttga tcgcgcgtaa gaaaaactta cacaacataa taaaaaattg 180 gagaataaaa tgtttaaaac taaacgttct ctgctgaact caagcattgc aatctcattt 240 gctgtacttg gcgtgcaagc gcaagcagag acactagaat tacaagcaga atcgtttgcc 300 aactctggcg gcacatatag tgatgggcag ccaaatccag ttactattta caatgtaaac 360 ggacaaggcg cgattaattt cgttaatgca ggtgactatg ttgattacaa tatcaatgcc 420 ctgggggggg aatacgatat tgaatatttt gttggcaccg gtgttacatc tggacccaat 480 atagaagtac ttgtggacgt taatggtaca tggcaaagtc aaggctcggt tgctgttcct 540 tatggtagtt gggatgactt ccaatcgctc acgccatctc atacagtaac attacctgtt 600 ggtacttcga ctgttcgctt gttggcggta ggctcaactt ggcaatggaa tttagaatca 660 tttagattaa ctcaagtttc acctgttgaa ccagtaggtg atgctgataa tgacggcgtt 720 aatgataatc aagatttatg tcctaatagc ccatcaggtg tcacagttga taataatggt 780 tgccaaatta ctggtggtac agatccgggt ggagaatcct ttgtaatcca gatggaggct 840 tttgattcaa ctggaagtga tgattcaaga gcaaaaggtg tgattattgg tgaacgtggt 900 tacccgcaag ataagcatac ggttgttgat agtgtacaaa ctactgattg ggtggattac 960 agtattaatt tcccatcttc agccaattat agtgtctcaa tgttagcgtc tgggcagaca 1020 gaccacgcca cggctgtatt atatcttgat ggcactgaaa ttaatgaagt gcctgtgcat 1080 acaggtagcc aagccgattt tgcaaacttt caactagcag gctcggttta tattgcgtct 1140 ggtacgcata ccattcgagt tcaagcacaa agctctactg gtgagtttag ctggttatgg 1200 tttggtgatg cattaacttt cactaactta gacagtgatg ggggtaatgg cggcgaggct 1260 acacaagatg ctgataacga tggagtgtta gatagctcag attcttgtcc aaatacgcct 1320 acgggtgagc ctgcagatgt gactggctgt agtgcatcgc aattagatga tgacaatgat 1380 ggtgtttcaa ataatgttga tcaatgtccg aatacagttg caggtacaga ggtagatgct 1440 gatggctgtg aagtaatatt tgcagatgca gataacgacg gcatagaaga ttcgcaagat 1500 ttttgcccga acacaccagc aggtgaagcg gtcaataatt caggttgtgg tgcgtcacaa 1560 ttggatgccg ataatgatgg cgttacaaac aatatagatc aatgtccaaa tacaccagcg 1620 ggcacacaag ttgatgcgtc aggttgtgag accgacaatg gtggtgagcc aggtgatagc 1680 tattatcaca atggtcaagg tttattgttt ggccgtgtag atggcgcaac taacttttta 1740 ggtgaagaag gctatgttgc caaccctgat aactatgatg tgacaaccga tttactcgaa 1800 actgatgatg caattcgcgc taattcaacc gaagtgtttc gtggtgagat ttatgacgca 1860 gatggccata ttgctttcta tgaacatata gatgatagcg tgcgtttgta tatagacggt 1920 caactcgtgc tttcaaatga tagctgggaa aactcgtctc aaaccacaga tttaaactta 1980 acacctggtt ggcataattt tgagttaaga cttggtaacg cagatggtgg cagtggtgct 2040 gtgagtggta taggttttgg tatagatgtg gacggtggga caaactttgt tcacccaagc 2100 aatttaagcc cgagtatgtt tagagcgagc ggccaagttg tggttgatcc aattttacca 2160 ccttcaggcg gaatttatat tcagctagaa gactttgacg aaaccggcac tgttggccga 2220 gtcgcgagcg atccaaatga cggctttgta aaaggtgatt caaacgttgg ttgggttacc 2280 aatggcgatt ggggtaaata ccacaatgta tttttagaag ctggtacata tagagcattc 2340 ataactgtat caacacctgc gggcggtagt tatggtgcac gtgttgatat agacggcgaa 2400 ccttttgctt ggggatattt tgacagcaca ggtggctggg atatcgcagc agaatatgag 2460 ttgtatggtg gtcatttagt cgtagaaagt acaggtaacc atacactaca tgttgaagct 2520 gttggtgggt ctgattggca atggagtggc gacttagttc gtttagctaa agtgagcgat 2580 agtgcagtca aacaaccgcg tgtatacaat ccgaacgagc atattgtcgc tgaaattcaa 2640 ggaccagcaa cgggtttaca atatttaaaa acaccagtgg aaattccatt agccaacaag 2700 gtattaaaat cagacgtttg gtatacctat ccgcaaaacc gaaacctagt cgttgatggt 2760 gacacaccat atgctgattt tggtgcaacg ggtgccttct gggggcatcc acctgaacac 2820 gatttctacg atgatactgt gatcatggat tgggcggtta atgttgttga tgatttccaa 2880 agtgaaggtt ttgaatatac tgcgcgcggt gagtttgact ggggctatgg ttggttcact 2940 gagtttacga ctaatccgca accgcactat gttcaaacat tggatggccg taatgtacgt 3000 atgacattca tgggctattt aagtcatgat ggttataaca acaattggct gagtaatcat 3060 agtccagcgt ttgtgccttt catgaagtca caagttgacc aaatattgaa agcaaatcct 3120 gacaagttga tgtttgatac acaaaccaat tctacacgct caaccgatat gcgcacattt 3180 ggtggagatt tctctcctta tgcaatggaa aacttccgtg tttggttgtt gaaaaaatat 3240 agcaatgcac agttagtctc tatgggtatt aatgatatta ctagctttga ttatggcgca 3300 tatttgagag cacaaggcat cacccatact gattggtcaa atgcagggga tactatttct 3360 ggcaatattc caatgatgga agacttcatt tattttaatc gtgatgtctg gaaccaaaag 3420 tttgcagaag tattagaata tattcgtcag caacgtccta atattgaaat tggtgcaagt 3480 actcacttgt ttgaatctcg tggttatatc tttaacgaga acattacctt cttatcgggt 3540 gaattgaact taggtgcaag aactagtatt tcagagttac ccactaatat tcttgttcac 3600 ttaaagggcg cgcaagcagt cgataaaacc ttggcttatt tcccataccc ttgggagttt 3660 gatgaattac gtattcaaaa tgcaccacgt tttggtcgag gttgggttgc tcaagcttat 3720 gcttatggtg gtttattctc tatcccagct aatgtgtggg tcggtggaga agtatttact 3780 tggtcaccgg gagcggataa ctatcgtgat atctatcaat ttgtgcgtgc acaagcgaac 3840 ttgttagatg gttatacgtc atatgcgaaa gcaggttatg ttcatgccat gttctcatca 3900 atgaaagcgg gttttattga tggtggtaac caagttcaat caagcgtgaa aatcttaact 3960 gaagataata tcaactttga tatgttagta tttggtgatg cgggttatcc tgttgtacca 4020 cgccaagctg actttgataa atttgaatat attttctacg acggtgactt aaactactta 4080 actgctgagc aacaagctgt attggatgct caaggcagta aggtgaagca tattggtcaa 4140 cgtggcacaa ttgctggttt acaaattaat gtaagcatta atggtagtgt gtctaacgaa 4200 accgtgtctg ctgtatctcg tattcacgaa acagactcaa cagcgcctta cgtagtacat 4260 ttgattaacc gtccgtttgc gggtggggta acaccaatac tgaacaatgt tgaagtggca 4320 attccagcaa gttacttccc acaaggggta acctcagcga aattacactt accagatggc 4380 tcaagttcta cagtcgcggt ttctactaac gccaatggtg atacagtcgt gtcggttagc 4440 aatcttgaag tttggggtat tttagagtta gctcactaat tttttgatac gatttttcat 4500 ggatttaagg ggcttcagtg aagccccttt tatttggtac ttgttgacta acgattgagg 4560 acatttttat ggcgaatcaa taaaccttat cttaaacaag cgtgtagtta atgtgttttg 4620 attacggttt ggtgactgtc acttttattt a 4651 // |
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