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EBI DbfetchID AF116581; SV 1; linear; genomic DNA; STD; PRO; 2991 BP. XX AC AF116581; XX DT 10-DEC-1999 (Rel. 62, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus subtilis alpha-amylase (amy) gene, complete cds. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2991 RX DOI; 10.1016/S0167-4838(00)00037-6 RX PUBMED; 10825545. RA Cho H.Y., Kim Y.W., Kim T.J., Lee H.S., Kim D.Y., Kim J.W., Lee Y.W., RA Lee S.B., Park K.H.; RT "Molecular characterization of a dimeric intracellular maltogenic amylase RT of Bacillus subtilis SUH4-2"; RL Biochim. Biophys. Acta 1478(2):333-340(2000). XX RN [2] RP 1-2991 RA Kim Y.W., Kim T.J., Kim J.W., Park K.H.; RT ; RL Submitted (24-DEC-1998) to the EMBL/GenBank/DDBJ databases. RL Food Science and Technology, Seoul National University, Seodun-Dong RL Kwonsun-Gu, Suwon, Kyungki-Do 441-744, Republic of Korea XX FH Key Location/Qualifiers FH FT source 1..2991 FT /organism="Bacillus subtilis" FT /strain="SUH4-2" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS 628..2607 FT /codon_start=1 FT /transl_table=11 FT /gene="amy" FT /product="alpha-amylase" FT /note="extracellular alpha-amylase" FT /db_xref="GOA:Q9R9H7" FT /db_xref="HSSP:1BAG" FT /db_xref="InterPro:IPR006046" FT /db_xref="InterPro:IPR006047" FT /db_xref="InterPro:IPR006048" FT /db_xref="InterPro:IPR013780" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/TrEMBL:Q9R9H7" FT /protein_id="AAF14358.1" FT /translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAP FT SIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQ FT PTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEIKSIPNWTH FT GNTQIKNWSDRWDVTQNSLLGLYDWNTQNTQVQSYLKRFLERALNDGADGFRFDAAKHI FT ELPDDGSYGSQFWPTITNTSAEFQYGEILQDSASRDAAYANYMDVTASNYGHSIRSALK FT NRNLGVSNLSHYASDVSADKLVTWVESHDTYANDDEESTWMSDDDIRLGWAVIASRSGS FT TPLFFSRPEGGGNGVRFPGKSQIGDRGSALFEDQAITAVNRFHNVMAGQPEELSNPNGN FT NQIFMNQRGSHGVVLANAGSSSVSINTATKLPDGRYDNKAGAGSFQVNDGKLTGTINAR FT SVAVLYPDDIAKAPHVFLENYKTGVTHSFNDQLTITLRADANTTKAVYQINNGPETAFK FT DGDQFTIGKGDPFGKTYTIMLKGTNSDGVTRAEEYSFVKRDPASAKTIGYQNPNHWSQV FT NAYIYKHDGGRAIELTGSWPGKPMTKNADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQ FT NQPGFDYVQNGLYNDSGLSGSLPH" XX SQ Sequence 2991 BP; 921 A; 571 C; 717 G; 781 T; 1 other; ggatcctccg ccattatgtt tgaatttccg tttaaagaat gggctgcaag ccttgtgttt 60 ttgttcatca ttatcttata ttactgcatc agggctgcgg catctggaat gctcatgccg 120 agaatggaca ccaaagaaga actgcaaaaa cgggtgaagc agcagcgaat agaatcaatt 180 gcggtcgcct ttgcggtagt ggtgcttacg atgtacgaca gggggattcc ccatacattc 240 ttcgcttggc tgaaaatgat tcttcttttt atcgtctgcg gcggcgttct gtttctgctt 300 cggtatgtaa ttgtgaagct ggcttacaga agagcggtaa aagaagaaat aaaaaagaaa 360 tcatcttgaa aaatagatgg tttctttttt tgtttggaaa gcgagggaag cgttcacagt 420 ttcgggcagc tttttttata ggaacattga tttgtattca ctctgccaag ttgttttgat 480 agagtgattg tgataattta aaatgtaagc gttaacaaaa ttctccagtc ttcacatcag 540 tttgaaagga ggaagcggaa gaatgaagta agagggattt ttgactccga agtaagtctt 600 caaaaaatca aataaggagt gtcaagaatg tttgcaaaac gattcaaaac ctctttactg 660 ccgttattcg ctggattttt attgctgttt catttggttc tggcaggacc ggcggctgcg 720 agtgctgaaa cggcgaacaa atcgaatgag cttacagcac cgtcgatcaa aagtggaacc 780 attcttcatg catggaattg gtcgttcaat acgttaaaac ataatatgaa ggatattcat 840 gatgcaggat atacagccat tcagacatct ccgattaacc aagtaaagga agggaaccaa 900 ggagataaaa gcatgtcgaa ctggtactgg ctgtatcagc cgacatcgta ccaaattggc 960 aaccgttact taggtactga gcaagaattt aaagaaatgt gtgcagccgc tgaagaatat 1020 ggcataaagg tcattgttga cgcggtcata aatcatacca ccagtgacta tgccgcgatt 1080 tccaatgaga ttaagagtat tccaaactgg acacatggaa acacacaaat taaaaactgg 1140 tcggatcgat gggatgtcac gcagaattca ttgctcgggc tgtatgactg gaatacacaa 1200 aatacacaag tacagtccta tctgaaacgg ttcttagaaa gagcattgaa tgacggggca 1260 gacggttttc gctttgatgc cgccaaacat atagaacttc cagatgatgg gagttacggc 1320 agtcaatttt ggccgactat cacaaataca tctgcagagt tccaatacgg agaaatcctg 1380 caggatagtg cctccagaga tgctgcatat gcgaattata tggatgtgac agcgtctaac 1440 tatgggcatt ccataaggtc cgctttaaag aatcgtaatc tgggcgtgtc gaatctctcc 1500 cactatgcat ctgatgtgtc tgcggacaag ctagtgacat gggtagagtc gcatgatacg 1560 tatgccaatg atgatgaaga gtcgacatgg atgagcgatg atgatatccg cttaggctgg 1620 gcggtgatag cttctcgttc aggcagtacg cctcttttct tttccagacc tgagggaggc 1680 ggaaatggtg tgaggttccc ggggaaaagc caaataggcg atcgcgggag tgctttattt 1740 gaagatcagg ctatcactgc ggtcaataga tttcacaatg tgatggctgg acagcctgag 1800 gaactctcga acccgaatgg aaacaaccag atatttatga atcagcgcgg ctcacatggc 1860 gttgtgctgg caaatgcagg ttcatcctct gtctctatca atacggcaac aaaattgcct 1920 gatggcaggt atgacaataa agctggagcg ggttcatttc aagtgaacga tggtaaactg 1980 acaggcacga tcaatgccag gtctgtagct gtgctttatc ctgatgatat tgcaaaagcg 2040 cctcatgttt tccttgagaa ttacaaaaca ggtgtaacac attctttcaa tgatcaactg 2100 acgattacct tgcgtgcaga tgcgaataca acaaaagccg tttatcaaat caataatgga 2160 ccagagacag cgtttaagga tggagatcaa ttcacaatcg gaaaaggaga tccatttggc 2220 aaaacataca ccatcatgtt aaaaggaacg aacagtgatg gtgtnacgag ggccgaggaa 2280 tacagctttg ttaaaagaga tccagcttcg gccaaaacca tcggctatca aaatccgaat 2340 cattggagcc aggtaaatgc ttatatctat aaacatgatg ggggccgggc aattgaattg 2400 accggatctt ggcctggaaa accaatgact aaaaatgcag atggaattta cacgctgacg 2460 ctgcctgcgg acacggatac aaccaacgcc aaagtgattt ttaataatgg cagcgcccaa 2520 gtgcccggcc agaatcagcc tggctttgat tacgtgcaaa atggtttata taatgactcg 2580 ggcttaagcg gttctcttcc tcattgaggg caatgctaga cgggacttac cgaaagaaac 2640 catctgtgat ggtttctttt ttgttcataa atcagacaaa acttttccct ggcaaaagtt 2700 tgtgaagtat tgcacaatat aaatgtgaaa tacttcacaa ataaaaagac atcaaagaga 2760 aacataccct ggaaggatga ttaatgatga acaaacatgt aaataaagta gctttaatcg 2820 gagcgggttt tgttggaagc agttatgcat ttgcgttaat taaccaaggg atcacagatg 2880 agcttgtggt cattgatgta aataaagaaa aagcaatggg cgatgtgatg gatttaaacc 2940 acggaaaggc cgttgcgcca caaccggtca aaacatctta cggaatctag a 2991 // |
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