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EBI DbfetchID AF115391; SV 1; linear; genomic DNA; STD; PRO; 6907 BP. XX AC AF115391; XX DT 03-JUN-1999 (Rel. 59, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Lactobacillus sakei LaaA (laaA) gene, partial cds; LaaB (laaB), putative DE acetate kinase AckA (ackA), LaaC (laaC) genes, complete cds; rbs operon, DE complete sequence; and LaaE (laaE) gene, partial cds. XX KW . XX OS Lactobacillus sakei OC Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. XX RN [1] RP 1-6907 RX PUBMED; 10941799. RA Stentz R., Zagorec M.; RT "Ribose utilization in Lactobacillus sakei: analysis of the regulation of RT the rbs operon and putative involvement of a new transporter"; RL J. Mol. Microbiol. Biotechnol. 1(1):165-173(1999). XX RN [2] RP 1-6907 RA Stentz R., Zagorec M.; RT ; RL Submitted (18-DEC-1998) to the EMBL/GenBank/DDBJ databases. RL Laboratoire de Recherches sur la Viande, INRA, Domaine de Vilvert, RL Jouy-en-Josas 78350, France XX FH Key Location/Qualifiers FH FT source 1..6907 FT /organism="Lactobacillus sakei" FT /strain="23K" FT /mol_type="genomic DNA" FT /db_xref="taxon:1599" FT CDS <1..538 FT /codon_start=2 FT /transl_table=11 FT /gene="laaA" FT /product="LaaA" FT /note="similar to dipeptidase" FT /db_xref="GOA:Q9X4L9" FT /db_xref="InterPro:IPR005322" FT /db_xref="UniProtKB/TrEMBL:Q9X4L9" FT /protein_id="AAD34332.1" FT /translation="EFDPMGHGADADKYRYRPIGLNRTQNAHILQLRNDVPKEQAAIMW FT ITLGMPTYTPFIPFFTNANDTDPSFSETPMKWDINSAYWMYRTISVLVEGHYSQHIQGN FT VDYLTSCKQELRTMLDSIEEEAKNYQGEALTKYLTEQNYLIVNTMKDKAMGMIGELVMS FT GINLSKLTFNMDHNL" FT CDS 824..1366 FT /codon_start=1 FT /transl_table=11 FT /gene="laaB" FT /product="LaaB" FT /note="similar to putative transcriptional regulator" FT /db_xref="GOA:Q9X4M0" FT /db_xref="InterPro:IPR000835" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/TrEMBL:Q9X4M0" FT /protein_id="AAD34333.1" FT /translation="MSSKITGKYSEDWEVITHLSLIYRWVISNLSDFVSGESLNYKVSF FT DQFLIMYEIATNDDLTGSKLAKTMSVSRSAISRQCRLLKENGYVVEEHLQSDQRVKFLS FT LTKQGQDVLDRLVSRYVQKYQEIETQMGREKIDGLIQFIDQFYLDVIEPSDYHDNKMGR FT SFLEKVQQIQSDDQIAK" FT CDS 1511..2695 FT /codon_start=1 FT /transl_table=11 FT /gene="ackA" FT /product="putative acetate kinase AckA" FT /db_xref="GOA:Q9X4M1" FT /db_xref="InterPro:IPR000890" FT /db_xref="InterPro:IPR004372" FT /db_xref="UniProtKB/Swiss-Prot:Q9X4M1" FT /protein_id="AAD34334.1" FT /translation="MEKILAINAGSSTLKWQLFEMPSETVIAKGMIDRLGLSDSVFTAK FT YGDNQKFKEVQDVTTHEMAATLLLTRLKELGIVSHLDEITGVGHRVVGGGEAFSDSMVI FT NPVALDEINRLAEYAPLHNPTQAYYIKIFTALLPGVPQVAVFDTSFYSTLAPENYLYSI FT PQEYYQTFGARKYGAHGTSHRYVAHRAAEILGTPLESQKMITLHLGSGASITAVQDGHA FT VDTSMGFTPLAGITMGTRSGDIDVSLVAFLAKKLEITMPEMIDILNHKSGLLGISELSP FT DMRDLEETAATRPQSALALSIFVNRVVKYVGSYVALMNGIDTLVFTAGSGENGSELRAD FT ICKQLACFGVKLDEEKNNVRSQERIISADDSKVKVLIVPTNEELMIARDVMRLK" FT CDS 2710..2955 FT /codon_start=1 FT /transl_table=11 FT /gene="laaC" FT /product="LaaC" FT /db_xref="UniProtKB/TrEMBL:Q9X4M2" FT /protein_id="AAD34335.1" FT /translation="MAQKPLFEFEVDIPRLSARLEDQPAVKTFFEALTPGYQREWARYV FT YGAKAEETQQRHYADMLMILEAGYKSKRGYSQAKKK" FT -35_signal 3037..3043 FT /gene="rbs operon" FT -10_signal 3061..3066 FT /gene="rbs operon" FT CDS 3130..4014 FT /codon_start=1 FT /transl_table=11 FT /gene="rbsU" FT /product="putative ribose transporter RbsU" FT /db_xref="GOA:Q9X4M3" FT /db_xref="InterPro:IPR004673" FT /db_xref="InterPro:IPR010651" FT /db_xref="UniProtKB/Swiss-Prot:Q9X4M3" FT /protein_id="AAD34336.1" FT /translation="MNAVNILIGLMPMIGWGIFPVIVGKIGGKPASQILGTTFGTLILA FT IVVAIFRGTPIPETKTFIFCLISGACWALAQIITFHVFETMGVSRTMPITTGFQLVGAS FT LWGVFVLGNWSSSQSKLIGFTAIALIIIGVYLTAWSEDKSSASKSGAVKGILLLLVGEL FT GYLGYSAFPQAVSADGFQGFLPQAIGMTIVGIIFGLTQTKKDYKPFKEATSYKNIFSGF FT FFAFAALTYLISAQPSVNGLATGFVLSQTSVIFATIGGIYILKEKKSKKEMIAVMVGLL FT LVLVAGSVTAFIK" FT CDS 4035..4430 FT /codon_start=1 FT /transl_table=11 FT /gene="rbsD" FT /product="ribose permease RbsD" FT /db_xref="GOA:Q38Z79" FT /db_xref="InterPro:IPR007721" FT /db_xref="UniProtKB/Swiss-Prot:Q38Z79" FT /protein_id="AAD34337.1" FT /translation="MRKTKVINTQISSVISDMGHFDTLSIGDAGMPVPVGTKKIDVAIE FT NGVPSFIQVLTNILSELEVQKVYLANEIKTANPEQLAAIKALIGETPIEFIDHSQMKQD FT LNKAKAFVRTGEMTPYSNIILESGVVF" FT CDS 4450..5358 FT /codon_start=1 FT /transl_table=11 FT /gene="rbsK" FT /product="ribokinase RbsK" FT /db_xref="GOA:Q9X4M5" FT /db_xref="HSSP:1RKD" FT /db_xref="InterPro:IPR002139" FT /db_xref="InterPro:IPR002173" FT /db_xref="InterPro:IPR011611" FT /db_xref="InterPro:IPR011877" FT /db_xref="UniProtKB/TrEMBL:Q9X4M5" FT /protein_id="AAD34338.1" FT /translation="MKKVIVLGSTNVDTILNVQRFPQPGETLAMDGRAVAGGGKGANQA FT IAAVRSGAQTAFISKVGKEGAADFMIDTFKKDGMNVDHVRCSETAGTGQAYIMVDAHGQ FT NSILIYGGANQDVTVEDVHEAETAIKAADRIVAQFEVPIPAIIEAFKIARANGVQTILN FT PAPAIKNIPTELLAVTDMIVPNETEAEIITGIKVTDEASMQANAEAMFKLGIKVVIITV FT GSKGSFYATPTATGFVPAFKVNAVDTTAAGDTFIGALSTKLELDYSNIEAAMTYANKAS FT SIAVQTKGAQNSIPYEKDIQL" FT CDS 5439..6446 FT /codon_start=1 FT /transl_table=11 FT /gene="rbsR" FT /product="rbs operon repressor RbsR" FT /db_xref="GOA:Q9X4M6" FT /db_xref="HSSP:1PRU" FT /db_xref="InterPro:IPR000843" FT /db_xref="InterPro:IPR001761" FT /db_xref="InterPro:IPR010982" FT /db_xref="UniProtKB/TrEMBL:Q9X4M6" FT /protein_id="AAD34339.1" FT /translation="MVRKVTIKDVAALAQTSVTTVSLILNNKGQRFSAETIARVRAARE FT KISYEPDYFAQNMVNKETKTIAVLVPDINNPFFSQFIQGVEEVAYQYDFIPLIYSAGQN FT NQKVNYYLKELVRRNVDGFILAVSHIEEETLEGHLKQRKIPYILLDQSEKQHQSDEIAV FT ADTEGGRMAANYLIEKGHRQIGIVLPEQRAWNIEQRFIGYQVALVSHQITVNPDWQITT FT PLSKQGGYQVAQQVIDSGVSAVFAVNDELAIGLARGLAELGHAVPESISIIGYDDIDWC FT EYVTPRLTTIKQPILALGQEATRLLLNRIEKPTLGMQQKMLSVELVERDSVQAQ" FT CDS 6605..>6907 FT /codon_start=1 FT /transl_table=11 FT /gene="laaE" FT /product="LaaE" FT /db_xref="InterPro:IPR005149" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/TrEMBL:Q9X4M7" FT /protein_id="AAD34340.1" FT /translation="MLSALMSRNMSGYKLRIILENAMLPRRKISNGVLYPLLDKLENKG FT CIGFDEADQDSRGTKIAHITEERRRYFAELMTKPVAHDAKWDDAYRFKMRGMHYID" XX SQ Sequence 6907 BP; 2190 A; 1220 C; 1523 G; 1974 T; 0 other; cgaattcgat ccaatgggtc acggcgctga tgctgacaag tatcgttacc gtccaattgg 60 cttaaacaga acacaaaatg cgcatatctt acaattgcgc aacgatgtgc ctaaagaaca 120 agctgcgatt atgtggatta ctttagggat gccaacttac acaccattta ttcccttctt 180 tacaaatgcc aatgatacag atcctagctt cagtgaaaca ccaatgaaat gggatattaa 240 tagtgcttat tggatgtacc ggacaatttc agtcttggtt gaaggtcact atagccaaca 300 tattcaaggt aacgttgact atctaacaag ctgtaaacaa gaattacgga caatgttgga 360 tagtattgaa gaagaagcta agaattatca aggcgaagca ctaacaaaat atttgacaga 420 acaaaattat ttgattgtta acacaatgaa agataaggcc atgggcatga ttggcgaact 480 cgtcatgtca ggaattaatt tatctaaatt gacattcaat atggatcaca atctataata 540 ggtttgaaag gagtgaacta ctaacgatag ttagtagttt gctctttttt tacaataacg 600 ccaaaataat agaaatttta ataaaaaagt tgcagataat tatccttgca ataattaacg 660 ttacaatata attgttaatc attaagtgac tttgtactaa attaatgaaa acaggtggtt 720 tcttttttga aagaatatgc tactatggtt gttagcaatc aaattaaaac cattaattat 780 tttcggccct catcacttgt atttataaaa aggagattca attatgtcat caaagattac 840 ggggaagtat tctgaagact gggaagttat cacccatcta tcacttatct atcgttgggt 900 tatcagcaat cttagcgatt ttgtcagtgg ggaaagtttg aactacaaag tgtcatttga 960 tcaattcttg atcatgtacg aaatcgccac caatgacgat ttaacgggta gcaaattagc 1020 taaaacaatg agtgttagtc gttctgcaat ctcacgtcaa tgtcgtttac ttaaagaaaa 1080 cggctacgtg gttgaagaac atttacaatc agatcaacgg gttaaattct tgagtttaac 1140 taaacaagga caagatgttt tagatcgctt agttagtcgt tatgtacaaa agtatcaaga 1200 aatcgaaaca caaatgggtc gggaaaaaat cgatggctta attcaattta tcgaccaatt 1260 ctatttagac gttattgaac ctagtgatta tcacgataat aaaatgggcc gttcattctt 1320 agaaaaagtc caacaaatcc aatcagacga ccaaattgcc aaataaataa atcaaaaggg 1380 acccttcgcc aaattcattt ttggtggcgg gtcctttttt aggtgttatt aaatcaaggg 1440 ttgtaacttc ctgtgaaatt gcttacaatt gtggcatgaa ggaattactt atagattggg 1500 agacgataac atggaaaaaa ttctagcaat taacgcgggc agctcaacac taaaatggca 1560 attatttgaa atgcccagtg aaacagttat cgccaaaggg atgatcgatc gcttaggttt 1620 atctgattcg gtgtttaccg ctaaatatgg tgacaatcaa aagtttaaag aagttcaaga 1680 tgtaaccact catgaaatgg cagcaacgct tttactcaca cgtttgaagg aattagggat 1740 tgttagtcat ttggatgaaa ttaccggtgt tggccaccga gtagttgggg gcggtgaagc 1800 tttcagtgat tcaatggtta ttaatccggt ggcattggac gagattaatc gcttggcaga 1860 atacgcgcca ttgcataacc ccacacaagc ttattacatc aagatcttta cggcgctact 1920 accaggtgtg ccccaagtag cggtttttga cacctctttt tacagtacct tggcacccga 1980 aaattattta tacagtattc cgcaagaata ttatcaaaca tttggcgcaa gaaaatacgg 2040 cgctcatggg accagtcatc gatacgttgc ccatcgtgcc gctgaaatct taggaacacc 2100 gctagaatct caaaaaatga ttacgctcca tttagggagt ggggcatcaa ttaccgcggt 2160 ccaagatggt catgccgttg atacttcaat gggttttacc ccactggctg ggattacaat 2220 gggcacacgt tctggcgata ttgacgtttc tttagttgct ttcttagcaa aaaaattaga 2280 aattacaatg cctgaaatga tcgatatttt aaatcacaaa tcaggtttgt taggtatttc 2340 agaactctca ccagatatgc gtgatttgga agaaaccgcc gctacacggc cacaatcagc 2400 gttggcatta tcaatctttg tcaatcgcgt cgttaaatat gtgggttcat atgtggcctt 2460 aatgaacgga atcgatacgt tagttttcac agccggtagt ggtgaaaacg ggagtgaatt 2520 acgggcagat atttgtaaac aacttgcttg ttttggtgta aaattggatg aagagaaaaa 2580 taatgtccgc agccaagaac ggattattag tgctgatgat tcgaaggtca aagttttaat 2640 tgtcccaacg aatgaagaat taatgattgc cagagatgtg atgcggttaa aataatggag 2700 tgattgaaga tggcgcaaaa accattattt gaatttgaag ttgatattcc aagattaagt 2760 gcgcgacttg aggaccagcc agccgtgaaa acttttttcg aggccttaac ccccgggtat 2820 caacgggaat gggcgcgcta tgtttacggc gctaaggctg aagaaacaca acaacgacat 2880 tatgctgata tgctgatgat tttagaagct ggttacaaat caaaacgcgg gtattcacaa 2940 gctaagaaaa aataaattta aaaaagatta attgggtgga aacggtgcct gattaatctt 3000 tttttatgtt taggttgtaa accgttttca aaaaaggttg aattatcgaa aaaggtcatt 3060 tataataatc atcaagatag ttagtaaaac gttttactaa ttgattaaat aggcttggag 3120 gaaattatga tgaacgctgt aaatatctta atcggcttaa tgccgatgat tggctggggg 3180 attttcccag ttattgttgg taaaattggt ggtaaaccag cgagtcaaat cctggggacc 3240 acttttggga cattaattct agcaattgtc gttgccattt tccgtgggac accaattcct 3300 gaaacgaaaa ctttcatttt ctgtttgatt tcaggtgcat gttgggcact ggcacaaatt 3360 attactttcc acgttttcga aacaatgggt gtttctagaa caatgccgat cacaactggc 3420 tttcaattag ttggggcatc tttgtgggga gtcttcgttc taggtaactg gtcaagttca 3480 caatcaaaat taatcggttt tacagcgatt gcattaatta tcatcggcgt ttatctcacc 3540 gcttggagcg aagataaatc aagtgctagc aagagtggtg cggttaaggg catcttgtta 3600 ttattagtcg gtgaattagg ctacttaggt tattcagcct ttccacaagc agttagcgct 3660 gatggtttcc aaggcttctt accacaagct attgggatga caattgtcgg cattatcttc 3720 ggcttaaccc aaactaaaaa agattataaa ccatttaaag aagcaacttc atataagaac 3780 atcttcagtg ggttcttctt cgcttttgcg gcattaactt acttgatttc agcacaacca 3840 tctgtcaacg gtttagcaac cggttttgtt ttatcacaaa ccagtgttat ctttgcgaca 3900 attggtggga tttacatctt aaaagaaaag aaatcaaaaa aagaaatgat cgccgtaatg 3960 gttggtttat tattagtctt ggtggcaggt tctgtcacag catttattaa ataaaaataa 4020 gggagattgg tgtcatgaga aaaactaagg tgattaacac acaaatttcg agcgttattt 4080 cagatatggg acatttcgat acattaagta ttggggatgc tggtatgcca gtaccagttg 4140 gtacgaagaa gattgatgtg gccattgaaa atggtgtacc gagttttatc caagttttaa 4200 caaatatctt aagtgaatta gaagtgcaaa aagtttactt agcaaatgaa attaaaacag 4260 caaatccaga acaattagcg gcgattaaag ctttaattgg ggagacacca atcgaattta 4320 ttgatcattc acaaatgaaa caagacttaa ataaggccaa agcatttgtc agaacaggtg 4380 aaatgacacc ttattccaac attattttag aaagtggcgt tgtcttttaa caaccaggag 4440 ggtattcaaa tgaaaaaagt aatcgtatta ggttcaacca atgtcgacac aattttaaac 4500 gttcaacgtt ttccacaacc tggcgaaacg ttagcaatgg atggccgcgc agttgctggt 4560 ggcggtaaag gtgctaacca agcaatcgca gctgttcgtt caggtgccca aacagctttc 4620 attagtaagg ttggtaaaga aggtgccgct gatttcatga tcgacacgtt caaaaaagac 4680 gggatgaacg ttgatcacgt gcgctgttca gaaacagctg ggacaggcca agcttatatc 4740 atggttgatg ctcacggcca aaatagtatt ttaatttacg gtggcgctaa ccaagacgtg 4800 actgttgaag atgttcacga agcagaaaca gccattaaag cagccgaccg aattgtggcg 4860 caattcgaag taccgatccc tgccattatc gaagctttca agattgcccg tgcaaacggc 4920 gttcaaacaa ttttgaaccc agcacctgcc atcaagaata ttccaaccga attattagca 4980 gtgacggaca tgattgtccc taacgaaact gaagcagaaa ttatcactgg cattaaagtg 5040 actgacgaag cttcaatgca agccaacgct gaagcaatgt tcaaattagg gatcaaagtg 5100 gtcattatca cggttggttc taaagggtca ttctatgcaa caccaactgc aactggtttt 5160 gtccctgcct ttaaggttaa cgcagtggat acaacagctg ctggtgatac tttcatcggt 5220 gcgttaagta caaaacttga attggattac agcaatatcg aagctgcgat gacttatgcc 5280 aataaggcgt catcaattgc cgtgcaaact aaaggtgcac aaaattcaat tccatatgaa 5340 aaggatattc agttataata gattaaaggg cgctccatgg cttaatcggc tgtgggcgtt 5400 tttaaatgat acggggtttt acaatggggg aagttaacgt ggtacgaaaa gtaacgatta 5460 aagatgtcgc tgccttggcc caaacatcgg tgacaactgt ttcactaatt ttaaataata 5520 agggccaacg ctttagtgca gaaacgattg cccgggtaag agcagcgcgt gaaaaaatca 5580 gctatgaacc ggattatttt gcccagaata tggtcaataa ggaaaccaaa acaattgcgg 5640 ttttagtacc ggatatcaat aatccttttt ttagtcagtt tattcaaggg gttgaagaag 5700 tcgcctatca gtacgatttt attccgttga tttatagtgc gggccagaat aatcagaaag 5760 ttaattatta tctgaaagag ctggtccgac gaaatgtcga tgggtttatt ttggcggttt 5820 cacatattga agaagaaacg ctagagggcc acttgaaaca acgtaaaata ccgtatattc 5880 tgttagatca atcggaaaag caacatcaaa gtgatgaaat cgctgtggcg gatacggaag 5940 gcggccgaat ggcggctaat tatctgattg aaaaagggca ccgtcaaatt gggatcgtgt 6000 tgccagaaca gcgggcttgg aatatcgagc agcgttttat tgggtaccaa gtagccttgg 6060 ttagtcatca aatcacggtt aatccggatt ggcaaataac gaccccctta tctaaacagg 6120 gaggctatca agttgcccag caggtgattg atagtggggt ctctgcggtc tttgccgtca 6180 atgatgagtt ggcgattggc ttagcacggg ggttagccga attaggtcat gcggtaccag 6240 agtcaatttc gattattggt tacgatgata ttgattggtg tgaatacgtc acaccgcgac 6300 taaccactat taaacaaccg attttggcgt tgggccaaga agcgacccgc ttgttgttaa 6360 atcggattga aaaacccacg ttagggatgc aacaaaaaat gttatcagtt gaattggttg 6420 aacgagatag tgttcaagcg caataaaaat taaattaagg accaaaaagt acgggcgaca 6480 ctaggcttgg caatttgcca accgaatgta ggccgtcttt ttttgtgctt tataaaaaaa 6540 gcttgcaaat atgtcggaaa gaccgattga aaagtgaggt tatgaggata tgtatgaatt 6600 gctgatgtta tcagccttaa tgagtcggaa tatgtcgggc tataagttgc ggattatctt 6660 ggaaaatgcc atgctgccca gacggaaaat ctcaaatggg gttttgtatc cgttgttaga 6720 taaattggaa aacaaaggct gcattggttt tgatgaagct gatcaggatt cacggggcac 6780 aaagattgcg cacatcacgg aagaacgccg gcgctatttt gctgaattaa tgactaaacc 6840 agtcgctcat gatgcgaagt gggatgacgc ttatcgcttt aagatgcgcg ggatgcacta 6900 tatcgat 6907 // |
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