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EBI DbfetchID AF113969; SV 1; linear; genomic DNA; STD; PRO; 5200 BP. XX AC AF113969; XX DT 04-MAY-1999 (Rel. 59, Created) DT 03-MAR-2000 (Rel. 62, Last updated, Version 3) XX DE Thermococcus hydrothermalis pullulanase (apuA) gene, complete cds. XX KW . XX OS Thermococcus hydrothermalis OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Thermococcus. XX RN [1] RP 1-5200 RX PUBMED; 10322035. RA Erra-Pujada M., Debeire P., Duchiron F., O'Donohue M.J.; RT "The type II pullulanase of Thermococcus hydrothermalis: molecular RT characterization of the gene and expression of the catalytic domain"; RL J. Bacteriol. 181(10):3284-3287(1999). XX RN [2] RP 1-5200 RA Erra-Pujada M., O'Donohue M.J., Duchiron F., Debeire P.; RT ; RL Submitted (14-DEC-1998) to the EMBL/GenBank/DDBJ databases. RL U.P.B.P.-Reims, Institut National de la Recherche Agronomique, Moulin de la RL Housse, Reims 51687, France XX FH Key Location/Qualifiers FH FT source 1..5200 FT /organism="Thermococcus hydrothermalis" FT /strain="AL662" FT /mol_type="genomic DNA" FT /db_xref="taxon:46539" FT CDS 370..4383 FT /codon_start=1 FT /transl_table=11 FT /gene="apuA" FT /product="pullulanase" FT /function="cleaves alpha-1,6- and alpha-1,4-glucosidic FT bonds" FT /db_xref="GOA:Q9Y8I8" FT /db_xref="InterPro:IPR004300" FT /db_xref="InterPro:IPR008960" FT /db_xref="InterPro:IPR011330" FT /db_xref="InterPro:IPR015922" FT /db_xref="InterPro:IPR019248" FT /db_xref="UniProtKB/TrEMBL:Q9Y8I8" FT /protein_id="AAD28552.1" FT /translation="MRRVVALFIAILMLGSIVGANVKSVGAAEPKPLNVIIVWHQHQPY FT YYDPVQDVYTRPWVRLHAANNYWKMAHYLSQYPEVHATIDLSGSLIAQLADYMNGKKDT FT YQIITEKIANGEPLTVDEKWFMLQAPGGFFDNTIPWNGEPITDPNGNPIRDFWDRYTEL FT KNKMLSAKAKYANFVTESQKVAVTNEFTEQDYIDLAVLFNLAWIDYNYITSTPEFKALY FT DKVDEGGYTRADVKTVLDAQIWLLNHTFEEHEKINLLLGNGNVEVTVVPYAHPIGPILN FT DFGWDSDFNDQVKKADELYKPYLGGGTAVPKGGWAAESALNDKTLEILAENGWEWVMTD FT QMVLGKLGIEGTVENYHKPWVAEFNGKKIYLFPRNHDLSDRVGFTYSGMNQQQAVEDFV FT NELLKLQKQNYDGSLVYVVTLDGENPVENYPYDGELFLTELYKKLTELQEQGLIRTLTP FT SEYIQLYGDKANKLTPRMMERLDLTGDNVNALLKAQSLGELYDMTGVKEEMQWPESSWI FT DGTLSTWIGEPQENYGWYWLYMARKALMENKDKMSQADWEKAYEYLLRAEASDWFWWYG FT SDQDSGQDYTFDRYLKTYLYEMYKLAGVEPPSYLFGNYFPDGEPYTTRGLVGLKDGEMK FT NFSSMSPLAKGVSVYFDGEGIHFIVKGNLDRFEVSIWEKDERVGNTFTRLQEKPDELSY FT FMFPFSRDSVGLLITKHVVYENGKAEIYGATDYEKSEKLGEATVKNTSEGIEVVLPFDY FT IENPSDFYFAVSTVKDGDLEVISTPVELKLPTEVKGVVIADITDPEGDDHGPGNYTYPT FT DKVFKPGVFDLLRFRMLEQTESYVMEFYFKDLGGNPWNGPNGFSLQIIEVYLDFKDGGN FT SSAIKMFPDGPGANVNLDPEHPWDVAFRIAGWDYGNLIILPNGTAIQGEMQISADPVKN FT AIIVKVPKKYIAINEDYGLWGDVLVGSQDGYGPDKWRTAAVDAEQWKLGGADPQAVING FT VAPRVIDELVPQGFEPTQEEQLSSYDANDMKLATVKALLLLKQGIVVTDPEGDDHGPGT FT YTYPTDKVFKPGVFDLLKFKVTEGSDDWTLEFHFKDLGGNPWNGPNGFSLQIIEVYFDF FT KEGGNVSAIKMFPDGPGSNVRLDPNHPWDLALRIAGWDYGNLIILPDGTAYQGEMQISA FT DPVKNAIIVKVPKKYLNISDYGLYTAVIVGSQDGYGPDKWRPVAAEAEQWKLGGADPQA FT VIDNLVPRVVDELVPEGFKPTQEEQLSSYDLEKKTLATVLMVPLVNGTGGEEPTPTESP FT TETTTTTPSETTTTTSTTTGPSSTTTSTPGGGICGPGIIAGLALIPLLLKRRN" XX SQ Sequence 5200 BP; 1363 A; 1480 C; 1457 G; 900 T; 0 other; attctcctag agtcttattg caacctcttc gcatcggtcg ccatcgtcga cggcgccagc 60 tacctccaga taatacggca cgtgctcctc cccatggcac tgccaggaat agcgaccgtt 120 gcaatattcg cattcatagg aggctggaca gagttcatcc tggcaagcct cctcctaacg 180 gagaagaacc agcccctttc agtgtggata tatctgctca tgagcggcat aggcagggga 240 atagactgga gctacttcgc cgcggctgca ctgctcttcg ccctgccggt gttcgtaatg 300 ttcatgcttg cacagaacta cataaggagc ggcctcacca tcggaggtct gaaggaatga 360 ggtgatatca tgaggcgggt ggttgccctc ttcattgcaa ttttgatgct tggaagcatc 420 gttggagcga acgttaagag cgttggcgcg gcggagccga agccgctcaa cgtcataata 480 gtctggcacc agcaccagcc ctactactac gaccctgtcc aggacgtcta caccaggccc 540 tgggtcaggc tccacgcggc gaacaactac tggaagatgg cccactacct gagccagtac 600 ccggaggttc acgccaccat tgacctctcg ggttcgctga tagcccagct tgccgactac 660 atgaacggca agaaggacac ctaccagata atcaccgaga agatagccaa cggggaaccc 720 ctcaccgtcg acgagaagtg gttcatgctc caggcaccgg gagggttctt cgacaacacc 780 atcccctgga acggtgaacc gataaccgac cccaacggca acccgataag ggacttctgg 840 gaccgctaca cggagctgaa gaacaagatg ctcagcgcaa aggccaagta cgcaaacttc 900 gtgactgaga gccagaaggt cgctgtgacg aacgagttca cagagcagga ctacatagac 960 ctagcggttc tcttcaatct cgcttggatt gactacaatt acatcacgag cacgccggag 1020 ttcaaggccc tctacgacaa ggttgacgag ggcggctata caagggcgga cgtcaaaacc 1080 gttctcgacg cccagatctg gcttctcaac cacaccttcg aggagcacga gaagataaac 1140 ctcctcctcg gaaacggcaa cgtcgaggtc acggtcgttc cctacgccca cccgataggc 1200 ccgatactca acgacttcgg ctgggacagc gacttcaacg accaggtcaa gaaggccgac 1260 gaactgtaca agccgtacct cggcggcggc accgcggttc caaaaggcgg atgggcggct 1320 gagagcgccc tcaacgacaa aactctggag atcctcgccg agaacggctg ggagtgggtc 1380 atgaccgacc agatggttct cggaaagctc ggcattgagg gaaccgtcga gaactaccac 1440 aagccctggg tggccgagtt caacggaaag aagatatacc tcttcccaag aaatcacgat 1500 ctaagtgaca gagttggctt tacctacagc ggaatgaacc agcagcaggc cgttgaggac 1560 ttcgtcaacg agctcctcaa gctccagaag cagaactacg atggctcgct ggtttacgtg 1620 gtcacgctcg acggcgagaa ccccgtggag aactacccct acgacgggga gctcttcctc 1680 accgaactct acaagaagct gaccgaactc caggagcagg gtctcataag aaccctcacc 1740 ccgagcgagt acatccagct ctacggcgac aaggccaaca agctcacacc tcggatgatg 1800 gagcgccttg acctcaccgg agacaacgtt aacgccctcc tcaaggccca gagcctcggc 1860 gaactctacg acatgaccgg cgttaaggag gagatgcagt ggcccgagag cagctggata 1920 gacggaaccc tctccacgtg gataggcgag ccccaggaga actacggctg gtactggctc 1980 tacatggcca ggaaggccct tatggagaac aaggataaaa tgagccaggc ggactgggag 2040 aaggcctacg agtacctgct ccgcgccgag gcaagcgact ggttctggtg gtacggaagc 2100 gaccaggaca gcggccagga ctacaccttc gaccgctacc tgaagaccta cctctacgag 2160 atgtacaagc tggcaggagt cgagccgccg agctacctct tcggcaacta cttcccggac 2220 ggagagccct acaccacgag gggcctggtc ggactcaagg acggcgagat gaagaacttc 2280 tccagcatgt ccccgctggc aaagggcgtg agcgtctatt tcgacggcga ggggatacac 2340 ttcatagtga aagggaacct ggacaggttc gaggtgagca tctgggagaa ggatgagcgc 2400 gttggcaaca cgttcacccg cctccaagag aagccggacg agttgagcta tttcatgttc 2460 ccattctcaa gggacagcgt tggtctcctc ataaccaagc acgtcgtgta cgagaacgga 2520 aaggccgaga tatacggcgc caccgactac gagaagagcg agaagcttgg ggaagccacc 2580 gtcaagaaca cgagcgaagg aatcgaagtc gtccttccct ttgactacat agaaaacccc 2640 tccgacttct acttcgctgt ctcgacggtc aaagatggag accttgaggt gataagcact 2700 cctgtggagc tcaagctccc gaccgaggtc aagggagtcg tcatagccga tataaccgac 2760 ccagaaggcg acgaccatgg gcccggaaac tacacttatc ccacggacaa ggtcttcaag 2820 ccaggtgttt tcgacctcct ccgcttcagg atgctcgaac agacggagag ctacgtcatg 2880 gagttctact tcaaggacct aggtggtaac ccgtggaacg gacccaacgg cttcagcctc 2940 cagataatcg aggtctacct cgacttcaag gacggtggaa acagttcggc cattaagatg 3000 ttccccgacg gaccgggagc caacgtcaac ctcgaccccg agcatccatg ggacgttgcc 3060 ttcaggatag cgggctggga ctacggaaac ctcatcatcc tgccgaacgg aacggccatc 3120 cagggcgaga tgcagatttc cgcagatccg gttaagaacg ccataatagt caaggttcca 3180 aagaagtaca tcgccataaa cgaggactac ggcctctggg gagacgtcct cgtcggctcg 3240 caggacggct acggcccgga caagtggaga acggcggcag tggatgcgga gcagtggaag 3300 cttggaggtg cggacccgca ggcagtcata aacggcgtgg ccccgcgcgt cattgatgag 3360 ctggttccgc agggctttga accgacccag gaggagcagc tgagcagcta cgatgcaaac 3420 gacatgaagc tcgccactgt caaggcgctg ctactcctca agcagggcat cgttgtgacc 3480 gacccggagg gagacgacca cgggccggga acgtacacct atccgacgga caaagttttc 3540 aagcccggtg ttttcgacct cctcaagttc aaggtgaccg agggaagcga cgactggacg 3600 ctggagttcc acttcaaaga cctcggtgga aacccgtgga acgggccgaa cggcttcagc 3660 ctgcagataa tcgaggtata cttcgacttc aaggagggcg ggaacgtctc ggccattaag 3720 atgttcccgg atgggcccgg aagcaacgtc cgtcttgatc caaatcaccc atgggacctg 3780 gcgcttagga tagccggctg ggactacgga aacctgataa ttctgcccga cggaaccgcc 3840 taccaaggcg agatgcagat ttccgcagat ccggttaaga acgccataat agtcaaggtt 3900 ccaaagaagt acctgaacat atccgactac ggactctaca ccgccgtcat cgtgggttcc 3960 caagacgggt acggcccgga caagtggagg cccgtggccg ctgaggccga gcagtggaag 4020 ctcggaggcg cagaccccca ggcggtcata gacaacctcg taccaagggt cgttgatgaa 4080 ctcgtgccgg agggcttcaa gccaacgcag gaggagcagc tgagcagcta cgaccttgag 4140 aagaagaccc tggcgacggt gctcatggta ccgctcgtca atgggactgg cggcgaggaa 4200 ccaacgccga cggagagccc aacggaaacg acgacaacca cacccagcga aacaaccacc 4260 acaacttcaa cgaccaccgg cccaagctca acgaccacca gcacacccgg cggaggaatc 4320 tgcggcccag gcattatagc gggcctggcc ctgataccgc tcctcctcaa gaggaggaac 4380 tgacccccat tttccattat tggaggtgag aacatggcgg aagtaaagct catcggtgtg 4440 tggaaagagt tcggggattt tactgcggtt aaggatatga accttgaagt taaggacggg 4500 gagttcatga ttctcctcgg accgagcggc tgcggaaaaa ctacgacgct cagaatgatt 4560 tcaggcctgg aagagccaac aagaggccag atttacattg gggataaact cgtcgcagac 4620 cccgaaaaag gcattttcat agcccccaag gacagagaca tagccatggt ctttcagagt 4680 tacgcattat acccccacat gacagtctac gacaacatcg ccttcccctt aaagctcagg 4740 aaagtaccga aacaggagat tgatcaaaga gtaagagaag tcgccgaaat gctcggctta 4800 accgaactcc tcaagagaaa acctaaagaa ttaagcggcg gtcaaaggca gagagtcgct 4860 ctggggaggg caatcgtcag aaaaccccac gtcttcctca tggacgagcc attgagcaac 4920 ctcgacgcaa aactcagagt aaaaatgcgc gcggaattga agagactcca aaagcaactc 4980 ggagtcacga ccatttacgt cacccacgat caagtggagg caatgacgat gggcgataga 5040 attgccgtga taaacgccgg agtcctccag caggtcggaa cgccggagga agtttacgac 5100 aaaccggcga acactttcgt tgcaggcttt attggctcac cacccatgaa cttcatggac 5160 gcgacaatca ccgaggatgg ttttgcagac ttcggagaat 5200 // |
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