EBI Dbfetch

ID   AF098955; SV 1; linear; genomic DNA; STD; PRO; 1441 BP.
XX
AC   AF098955;
XX
DT   02-JAN-2000 (Rel. 62, Created)
DT   05-FEB-2005 (Rel. 82, Last updated, Version 2)
XX
DE   Cytophaga drobachiensis beta-agarase B precursor (agaB) gene, complete cds.
XX
KW   .
XX
OS   Zobellia galactanivorans
OC   Bacteria; Bacteroidetes; Flavobacteria; Flavobacteriales;
OC   Flavobacteriaceae; Zobellia.
XX
RN   [1]
RP   1-1441
RX   PUBMED; 15456406.
RA   Jam M., Flament D., Allouch J., Potin P., Thion L., Kloareg B., Czjzek M.,
RA   Helbert W., Michel G., Barbeyron T.;
RT   "The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia
RT   galactanivorans: two paralogue enzymes with different molecular
RT   organizations and catalytic behaviours";
RL   Biochem. J. 385(Pt 3):703-713(2005).
XX
RN   [2]
RP   1-1441
RA   Barbeyron T., Flament D., Potin P., Kloareg B.;
RT   ;
RL   Submitted (14-OCT-1998) to the EMBL/GenBank/DDBJ databases.
RL   Centre d'Etudes Oceanographiques et de Biologie Marine, CNRS, Place Georges
RL   Teissier, Roscoff, Finistere/Bretagne 29250, France
XX
DR   StrainInfo; 163368; Dsij.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1441
FT                   /organism="Zobellia galactanivorans"
FT                   /strain="Dsij"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:63186"
FT   gene            125..1374
FT                   /gene="agaB"
FT   -35_signal      125..130
FT                   /gene="agaB"
FT                   /note="putative"
FT   -10_signal      148..153
FT                   /gene="agaB"
FT                   /note="putative"
FT   RBS             176..188
FT                   /gene="agaB"
FT                   /note="putative; similar to Omega sequence"
FT   CDS             197..1258
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="agaB"
FT                   /product="beta-agarase B precursor"
FT                   /function="hydrolyzes beta-1,4 linkage of
FT                   poly(O-beta-D-galactopyranosyl(1->4)-3,
FT                   6-anhydro-L-galactose"
FT                   /EC_number="3.2.1.81"
FT                   /note="galactoside hydrolase; AgaB; probable plasma
FT                   membrane lipoprotein; belongs to glycoside hydrolase family
FT                   16; similar to product presented by SwissProt Accession
FT                   Number P07883; similar to product encoded by GenBank
FT                   Accession Number M73783"
FT                   /db_xref="GOA:Q9RGX8"
FT                   /db_xref="InterPro:IPR008985"
FT                   /db_xref="InterPro:IPR013320"
FT                   /db_xref="PDB:1O4Z"
FT                   /db_xref="UniProtKB/TrEMBL:Q9RGX8"
FT                   /protein_id="AAF21821.1"
FT                   /translation="MYLIYLRLVFCCALLLGCGDNSKFDSATDLPVEQEQEQETEQEGE
FT                   PEESSEQDLVEEVDWKDIPVPADAGPNMKWEFQEISDNFEYEAPADNKGSEFLEKWDDF
FT                   YHNAWAGPGLTEWKRDRSYVADGELKMWATRKPGSDKINMGCITSKTRVVYPVYIEARA
FT                   KVMNSTLASDVWLLSADDTQEIDILEAYGADYSESAGKDHSYFSKKVHISHHVFIRDPF
FT                   QDYQPKDAGSWFEDGTVWNKEFHRFGVYWRDPWHLEYYIDGVLVRTVSGKDIIDPKHFT
FT                   NTTDPGNTEIDTRTGLNKEMDIIINTEDQTWRSSPASGLQSNTYTPTDNELSNIENNTF
FT                   GVDWIRIYKPVEK"
FT   sig_peptide     197..250
FT                   /gene="agaB"
FT                   /note="probable signal peptidase II substrate"
FT   mat_peptide     251..1255
FT                   /gene="agaB"
FT                   /product="beta-agarase B"
FT   misc_feature    746..748
FT                   /gene="agaB"
FT                   /note="probable catalytic nucleophile; active site"
FT   misc_feature    761..763
FT                   /gene="agaB"
FT                   /note="probable catalytic proton donor; active site"
FT   terminator      1326..1374
FT                   /gene="agaB"
FT                   /note="probable Rho-independent; stem loop"
XX
SQ   Sequence 1441 BP; 428 A; 275 C; 334 G; 404 T; 0 other;
     cgtctttatc acaattctat cttagaattc ttactaatgc tgacaaaact acggctgcaa        60
     ccgtgtatta cgataacttc tctatcattg aaaaagaaga gaggccataa caattgttga       120
     gtgtttgaga tagagggaga attgaaatat tctccctttt tatccttttt tcattttaaa       180
     caaattacgt ataaacatgt atttaatata tcttaggttg gtcttttgct gtgccctttt       240
     gttggggtgt ggcgacaatt caaaatttga tagtgcaacg gatttgccgg ttgaacaaga       300
     acaagaacag gaaacggaac aagagggaga acccgaagaa agttcggagc aagaccttgt       360
     cgaggaggtc gattggaagg atattcccgt acccgccgat gcaggaccga atatgaagtg       420
     ggagtttcaa gagatttccg ataattttga atatgaggcc cctgcggata ataaggggag       480
     tgaatttctc gaaaagtggg acgattttta tcacaatgcc tgggcaggcc cagggctgac       540
     cgaatggaaa cgggacaggt cctatgtagc cgatggcgag ctaaagatgt gggcgacaag       600
     aaaaccgggc tccgataaaa taaacatggg gtgcattact tctaagaccc gagtggtcta       660
     tcctgtttat attgaagcaa gggcaaaggt catgaactct accttggctt cggatgtttg       720
     gctcttaagt gccgatgaca cccaagagat agatattcta gaggcatatg gggccgatta       780
     ttccgaaagt gccggaaagg atcattccta tttttctaaa aaggtacaca taagccatca       840
     cgtctttatt cgagacccat ttcaagatta tcaaccaaag gatgccggtt cttggttcga       900
     agacggcacc gtctggaaca aagagttcca taggtttggt gtgtattgga gggatccatg       960
     gcatctagaa tattacatag acggtgttct ggtgaggacc gtttcgggaa aggacattat      1020
     cgaccccaaa cactttacga atacaacgga tcccggtaat acggaaatcg atacccgcac      1080
     cggtctcaat aaagaaatgg atattattat caatacagaa gaccaaactt ggcggtcttc      1140
     accggcctcg ggtttacagt ctaataccta tacgccaacg gacaatgaat tgagcaatat      1200
     agaaaacaat acgttcgggg tcgattggat caggatctat aaacctgtag agaaataaga      1260
     aaatccttct tttgctttgg tcgcgcccgt gagcttatga ttcggcgctg tctaaatagt      1320
     tttataaaac cataggtagt tccccctttg ttcaaactac ttgcctatgg ttttttttat      1380
     gttttattcc agaaagatga ctggggtcat atgatgttat ttatcttttt cttcccataa      1440
     a                                                                      1441
//