EBI Dbfetch

ID   AF098954; SV 1; linear; genomic DNA; STD; PRO; 2037 BP.
XX
AC   AF098954;
XX
DT   02-JAN-2000 (Rel. 62, Created)
DT   05-FEB-2005 (Rel. 82, Last updated, Version 3)
XX
DE   Cytophaga drobachiensis beta-agarase A precursor (agaA) gene, complete cds.
XX
KW   .
XX
OS   Zobellia galactanivorans
OC   Bacteria; Bacteroidetes; Flavobacteria; Flavobacteriales;
OC   Flavobacteriaceae; Zobellia.
XX
RN   [1]
RP   1-2037
RX   PUBMED; 15456406.
RA   Jam M., Flament D., Allouch J., Potin P., Thion L., Kloareg B., Czjzek M.,
RA   Helbert W., Michel G., Barbeyron T.;
RT   "The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia
RT   galactanivorans: two paralogue enzymes with different molecular
RT   organizations and catalytic behaviours";
RL   Biochem. J. 385(Pt 3):703-713(2005).
XX
RN   [2]
RP   1-2037
RA   Barbeyron T., Flament D., Potin P., Kloareg B.;
RT   ;
RL   Submitted (14-OCT-1998) to the EMBL/GenBank/DDBJ databases.
RL   Centre d'Etudes Oceanographiques et de Biologie Marine, CNRS, Place Georges
RL   Teissier, Roscoff, Finistere/Bretagne 29250, France
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2037
FT                   /organism="Zobellia galactanivorans"
FT                   /strain="Dsij"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:63186"
FT   gene            78..1852
FT                   /gene="agaA"
FT   -35_signal      78..83
FT                   /gene="agaA"
FT                   /note="putative"
FT   -10_signal      99..104
FT                   /gene="agaA"
FT                   /note="putative"
FT   misc_feature    145..148
FT                   /gene="agaA"
FT                   /note="similar to Epsilon sequence; putative enhancer of
FT                   translation"
FT   RBS             158..167
FT                   /gene="agaA"
FT                   /note="putative; similar to Omega sequence"
FT   CDS             168..1787
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="agaA"
FT                   /product="beta-agarase A precursor"
FT                   /function="hydrolyzes beta-1,4 linkage of
FT                   poly(O-beta-D-galactopyranosyl(1->4)-3,
FT                   6-anhydro-L-galactose"
FT                   /EC_number="3.2.1.81"
FT                   /note="galactoside hydrolase; AgaA; preproprotein; belongs
FT                   to glycoside hydrolase family 16; similar to product
FT                   presented by SwissProt Accession Number P07883; similar to
FT                   product encoded by GenBank Accession Number M73783"
FT                   /db_xref="GOA:Q9RGX9"
FT                   /db_xref="InterPro:IPR013320"
FT                   /db_xref="PDB:1O4Y"
FT                   /db_xref="UniProtKB/TrEMBL:Q9RGX9"
FT                   /protein_id="AAF21820.1"
FT                   /translation="MKKNYLLLYFIFLLCGSIAAQDWNGIPVPANPGNGMTWQLQDNVS
FT                   DSFNYTSSEGNRPTAFTSKWKPSYINGWTGPGSTIFNAAQAWTNGSQLAIQAQPAGNGK
FT                   SYNGIITSKNKIQYPVYMEIKAKIMDQVLANAFWTLTDDETQEIDIMEGYGSDRGGTWF
FT                   AQRMHLSHHTFIRNPFTDYQPMGDATWYYNGGTPWRSAYHRYGCYWKDPFTLEYYIDGV
FT                   KVRTVTRAEIDPNNHLGGTGLNQATNIIIDCENQTDWRPAATQEELADDSKNIFWVDWI
FT                   RVYKPVAVSGGGNNGNDGATEFQYDLGTDTSAVWPGYTRVSNTTRAGNFGWANTNDIGS
FT                   RDRGASNGRNNINRDINFSSQTRFFTQDLSNGTYNVLITFGDTYARKNMNVAAEGQNKL
FT                   TNINTNAGQYVSRSFDVNVNDGKLDLRFSVGNGGDVWSITRIWIRKVTSNSANLLAAKG
FT                   LTLEDPVETTEFLYPNPAKTDDFVTVPNSEIGSSIIIYNSAGQVVKKVSVVSENQKISL
FT                   EGFAKGMYFINLNGQSTKLIVQ"
FT   sig_peptide     168..224
FT                   /gene="agaA"
FT                   /note="probable signal peptidase I substrate"
FT   misc_feature    225..1784
FT                   /gene="agaA"
FT                   /note="encodes beta-agarase A proprotein"
FT   mat_peptide     225..1052
FT                   /gene="agaA"
FT                   /product="beta-agarase A"
FT   misc_feature    606..608
FT                   /gene="agaA"
FT                   /note="probable catalytic nucleophile; active site"
FT   misc_feature    621..623
FT                   /gene="agaA"
FT                   /note="probable catalytic proton donor; active site"
FT   terminator      1808..1852
FT                   /gene="agaA"
FT                   /note="stem loop"
XX
SQ   Sequence 2037 BP; 643 A; 358 C; 449 G; 587 T; 0 other;
     gattattctt actaatattg taggaaaatt taacacaaaa aacatctttg ttcagttttt        60
     gtcgagttgg taaaacctag aaaacagaca cggcattgta tatttggcga tgattcatct       120
     gtttgtttgt tgaatacatt tttattaacc cctaaaatta cattatcatg aaaaaaaatt       180
     atcttttact gtattttatt tttcttttgt gtggctctat cgctgcacag gactggaacg       240
     gaattcctgt acctgccaat cccggaaatg gtatgacttg gcaattacag gataatgttt       300
     cggatagttt taattacaca agtagtgaag gaaataggcc tactgccttt actagtaaat       360
     ggaaaccttc ctatatcaat ggatggactg gtcctggatc aacaattttt aatgccgcgc       420
     aggcatggac caatggttct caattggcaa ttcaggcaca accagcaggg aatggaaaat       480
     cttacaacgg aattatcacc tccaaaaata agatccagta cccggtgtat atggaaatta       540
     aggccaagat aatggaccag gtactagcaa atgctttctg gaccttgact gacgacgaga       600
     ctcaggaaat tgatattatg gaaggctatg gcagtgatcg ggggggaact tggttcgccc       660
     aaagaatgca tttgagccac catacattta ttcgtaaccc ctttacggat tatcagccta       720
     tgggagacgc tacatggtat tacaacggag gtacaccatg gcgttcagca tatcaccgtt       780
     atggatgtta ttggaaagat ccatttacat tggaatatta tattgacggg gtaaaggtta       840
     gaacggtcac aagagccgaa attgatccta ataatcatct cggcggaaca gggttgaatc       900
     aggcaacaaa tattattatt gattgtgaaa atcaaacaga ttggaggccc gcggctactc       960
     aagaagaact ggccgatgat agcaaaaata tcttctgggt cgattggata cgtgtgtaca      1020
     agcctgttgc ggtaagtgga ggtggaaaca acggtaacga cggtgccact gaatttcaat      1080
     atgatttagg aacggacacc tcggcagtat ggccagggta tacacgggtt tccaacacca      1140
     ctagggctgg taattttgga tgggcgaaca ccaatgacat cggatcaaga gatcgtgggg      1200
     cttctaacgg aaggaacaat ataaaccgtg atattaattt tagttcacaa actaggttct      1260
     tcactcaaga cctatccaat ggcacttata acgtattgat cacttttggg gacacctatg      1320
     cccgaaaaaa tatgaacgtc gcggccgaag ggcaaaataa attaacaaac ataaacacca      1380
     atgccgggca atatgttagt aggtcgtttg acgtaaatgt caacgacgga aaactagatt      1440
     tgcgattttc agttggtaat ggcggggatg tgtggtccat tacaagaatc tggattagaa      1500
     aagttacgag caacagcgct aatttgttag cggcaaaagg attaacattg gaagatcctg      1560
     tggaaactac ggaattttta tatcctaacc ccgcaaaaac agatgatttt gtgactgttc      1620
     ccaatagtga aattggaagt agtataatca tctataatag tgcaggtcaa gtagtgaaaa      1680
     aagtaagtgt ggtttccgaa aatcagaaaa tatcactaga aggatttgct aaaggaatgt      1740
     actttatcaa tttgaatggt cagagtacaa aacttattgt ccaataaaca caataacaat      1800
     ttcaattaaa cgacaaaggc gctctgatga tacagaaagg cctttgtcgt tttttaagtt      1860
     acttcaggaa ccaagataaa tttttaggtg gtattgttag cttcttctaa ctagaatatg      1920
     atctgtgttt tgcgggcttc ttgtacttgc tgtaaccgct tcgtttttgt gcaatgtcgg      1980
     cacatggtgt atgccctgtt tactgggtaa attaggtact tttctttttg aagctta         2037
//