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EBI DbfetchID AF096862; SV 1; linear; genomic DNA; STD; PRO; 8128 BP. XX AC AF096862; XX DT 17-MAY-1999 (Rel. 59, Created) DT 16-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Fervidobacterium pennivorans pullulanase type I (pulA) and isoleucyl-tRNA DE synthase (ileS) genes, complete cds. XX KW . XX OS Fervidobacterium pennivorans OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Fervidobacterium. XX RN [1] RP 1-8128 RX PUBMED; 10224005. RA Bertoldo C., Duffner F., Jorgensen P.L., Antranikian G.; RT "Pullulanase type I from Fervidobacterium pennavorans Ven5: cloning, RT sequencing, and expression of the gene and biochemical characterization of RT the recombinant enzyme"; RL Appl. Environ. Microbiol. 65(5):2084-2091(1999). XX RN [2] RP 1-8128 RA Duffner F.M., Jorgensen P.L., Antranikian G.; RT ; RL Submitted (02-OCT-1998) to the EMBL/GenBank/DDBJ databases. RL Screening Biotechnology, Novo Nordisk A/S, Novo Alle, Bagsvaerd 2880, RL Denmark XX DR CABRI; DSM 9078. DR StrainInfo; 161297; DSM 9078. XX FH Key Location/Qualifiers FH FT source 1..8128 FT /organism="Fervidobacterium pennivorans" FT /strain="DSM 9078" FT /mol_type="genomic DNA" FT /db_xref="taxon:93466" FT CDS 651..3200 FT /codon_start=1 FT /transl_table=11 FT /gene="pulA" FT /product="pullulanase type I" FT /function="debranching enzyme specific for alpha 1,6 FT glucosidic linkages in pullulan" FT /note="EC 3.2.1.41" FT /db_xref="GOA:Q9XDB5" FT /db_xref="InterPro:IPR004193" FT /db_xref="InterPro:IPR005323" FT /db_xref="InterPro:IPR006047" FT /db_xref="InterPro:IPR011840" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/TrEMBL:Q9XDB5" FT /protein_id="AAD30387.1" FT /translation="MFRDSSMGLLKKLSVITLIVFALSISFAETELIIHYHRWDGNYDG FT WNLWIWWVEPISKEGAAYQFTEKDDFGVVAKVKFPETLTKVGIIVRLGEWREKDVAMDR FT FITIKDGKAEVWLLQGIEQIYTTKPDTSPRVLFAQARDQYTIEAYLTGQVDTTKVGAKV FT TVDGQPLKIARVEKANPTDISRTNHVKVVLAEPIKLEDVNKDVQVEIEGYKPARVIMME FT ILDKIYYDGPLGFEYTPEKTTIRVWSPVSKTVDVLLYKNWDDKEPTQVVPMKYIGNGAW FT EAVLEGNWDGWFYKIRYFSYGEYRESVDYFSKAVTKNSAKSAIIDFSKTNPESWEKVAR FT PPLVAPEDAIIYEIHIADMTGLDNSGVKNKALYLGLTEKGTRGPNGVTTGLDHLVELGV FT THVHILPMFDFWTGDEADKDFERSYNWGYDPYLFTVPEGRYSTDPINPYTRIIEVKQMV FT KALHENGIRVILDMVFPHTWGVGVMSPFDQAVPYYFYRIDKTGAYLNESGCGNVIASER FT PMMRKYIVDTLKWWVTEYKIDGFRFDQMGLMDKVTMLAIKSELSKIEPSVVLYGEPWGG FT WGAPIRFGKADVGGTGIAAFNDEFRDALRGSVFNATVKGFLMGALAKETGVKRGVAGSI FT EYDEVIRSFAKDPQETINYVEVHDNHTLWDKNYLAAQADTTVKWTEEMLKDAQKLAGAI FT LLTSQGIPFLHAGQDFARTKKFDENSYKSPISINGLDYARKAEFIDVFNYYKGLIELRK FT SHIAFRQRTAEDIRKKLTFLPSPRKMVAFVLKDEKDPWKEILVIYNGDTKDQQFTLPDG FT TWNVVVDKDNAGTKVLYQVSGKINIKAISAMVMYKGN" FT CDS 3778..6471 FT /codon_start=1 FT /transl_table=11 FT /gene="ileS" FT /product="isoleucyl-tRNA synthase" FT /db_xref="GOA:Q9XDB4" FT /db_xref="InterPro:IPR001412" FT /db_xref="InterPro:IPR002300" FT /db_xref="InterPro:IPR002301" FT /db_xref="InterPro:IPR009008" FT /db_xref="InterPro:IPR009080" FT /db_xref="InterPro:IPR010663" FT /db_xref="InterPro:IPR013155" FT /db_xref="InterPro:IPR014729" FT /db_xref="InterPro:IPR015905" FT /db_xref="InterPro:IPR018353" FT /db_xref="UniProtKB/Swiss-Prot:Q9XDB4" FT /protein_id="AAD30388.1" FT /translation="MKANLVNKEPEMLKFWEEKEIYKKTLETRANAPTYLLHDGPPYAN FT GDIHLGTAMNKILKDFVTRYKTMRGYRVPFVPGWDTHGLPIEHRVTTSLGEEAKKKSPA FT EIRKLCKEFALKYVDIQREEFKRLGVKGDWEHPYITLDPDYEYHILDVFKTLVENGNVY FT RGNKPVYWCPTCRTALAEAEIEYHDHESPSIYVKFQMVDKPDTYIVIWTTTPWTIPANV FT AIALHPDYTYVKIKVDEEYWIVAEGLLQKFAADVGIDFEVVEKFVGKELEGKLTKHPLY FT DRTSVVVLADYVTLEDGTGCVHTAPGHGEEDYQTGLKYNLPVLSPVDDEGRFTKEAGKY FT EGLKIWDANKVIVEDLKNNGSLIKAGKISHSYPHCWRCKGPVMFRATPQWFISVDKNNL FT RGKVLEEIKKVKWYPAWGETRITAMVQERPDWTISRQRVWGVPIPAVKCKDCGEVTLEP FT KVIEHFANIVKEKGTDAWFELDVNELIPADFKCPACGSKNFEKTHDTLDVWMDSGCSWE FT AVIRSKGERFPVDLYLEGDDQHRGWFQSSIFLSTAKAGTAPFKAVVTHGFIKDEQGRKM FT SKSLGNVIDPMEIVNKYGADILRLWVASTDFFDNIRVGKNIIEQQVEVYRKLRNTLRYL FT LSNLYDFTEADLLPYEKLLPLDKWALGRLQKFIEQITQYYEGFEYSKVYNATVKYCTTE FT LSAVYLDILKDRLYVEAKDSIYRRSAQTALHYILEALIKILAPIIPFTAEEAYQESHLK FT RYESVHLEYWPEVRKEFIDEALLEEFDHLLLIRDDVLKALENARASDIIGHSLDAHVII FT EAKNEELKNLLRKYESLLEEFFIVSKVTLSENISGLNGQFANVLVQRAEGQKCQRCWKY FT HPDTGKDLEHPETCPRCSAVLRGERK" XX SQ Sequence 8128 BP; 2754 A; 1553 C; 1734 G; 2085 T; 2 other; ctgcaggtcg acggatcaat ctggttccat cttgtaattt ttcctgtgta satatccttt 60 atttgctgta tggtaaggtt cttgataggc aaatccttgt gaacaactat tgcgatacca 120 tcccaagcaa caagaactgg tgcaaaccac tttttcattt caaacatctt ttgaacttca 180 gaatccttaa accatctact ggagtttgcg atgtccgttg ttccgttaaa aagtgctgca 240 atccccgttg aactacctgc cccctcaagt gtaactttga tgtctggatt ctgtttgttg 300 aactcttcga cccacaactg cgcaatgtca agcatcgtgt tcgaaccttt tataaccaac 360 gcagtcgcaa aaccaaaaac cgcaaaaagt gaaacaaatg ctatcgttaa aatgaacttc 420 ctcatacctt ccacctcctg ttttaaagtt cgcttttatt ttctcagagg tccattaagt 480 aaaattttta atatgttaag aattgttaag ttcgattatt tgaggtgctg ctcgtctctt 540 ttttcactgg aaacatttcc aaaaatttgt agaaaaaatc tgggataatg tataatactt 600 tgggtacaat atagttgttc aaaaacacca atttaaggag gtgtcagggt atgtttagag 660 attcaagtat gggtctgctc aaaaagcttt cagttattac gctcatcgta ttcgcattat 720 cgataagctt tgcggaaaca gagctgatta tccattatca caggtgggat ggtaactacg 780 acggatggaa cctttggatt tggtgggttg aacctatttc aaaagaaggt gcagcttatc 840 agttcacaga aaaagatgat tttggagttg ttgcaaaagt taaattcccg gagacattaa 900 cgaaagttgg aataatagtt agacttggag aatggagaga aaaagatgtt gcaatggata 960 gattcataac aatcaaagat ggtaaggcgg aggtgtggct cttgcaagga atagaacaga 1020 tttacacaac aaaaccagac acaagccctc gtgtgctctt tgcacaggct agggaccaat 1080 acacaatcga agcatattta acaggtcaag tcgacacaac aaaagtcggt gcaaaagtta 1140 cggtagacgg tcaaccactc aaaatagctc gtgtcgagaa agccaatccg acggatattt 1200 ccagaacaaa ccatgtcaaa gttgttctcg ctgaacctat caaacttgag gatgttaaca 1260 aagatgtcca agtagagata gaagggtaca aaccggcaag ggtcatcatg atggaaattt 1320 tggacaagat ttattacgat ggtccactcg gatttgaata cacccctgaa aagacaacga 1380 taagggtttg gtcacctgtt tcaaaaactg ttgatgtgct actttacaaa aattgggatg 1440 ataaagaacc aacgcaagtt gttccaatga aatatatagg caacggggca tgggaagccg 1500 tccttgaagg aaactgggat ggatggttct acaaaatcag gtacttcagc tatggagaat 1560 atagagaatc agttgattac ttctcaaaag cggtaacgaa gaattctgcc aagagtgcta 1620 tcattgactt cagcaaaaca aatccagaga gctgggaaaa agtcgcacga ccaccactag 1680 ttgccccaga agatgcgata atatacgaaa tccacatcgc agatatgacc ggactcgata 1740 attctggcgt caagaacaaa gcactttact tgggactcac agaaaaaggc acaagaggtc 1800 ctaatggtgt aacaactggt cttgaccacc tcgttgaact cggggttact cacgttcata 1860 ttcttccaat gttcgatttc tggacaggcg atgaagctga taaagatttt gaaagaagtt 1920 acaactgggg ttatgaccca tacctcttta cagttccaga aggacgctac tcaacagacc 1980 caatcaaccc atacacaaga attatcgaag tcaaacaaat ggttaaagca ctccatgaaa 2040 atggtataag ggttatcctt gatatggtct tcccacatac atggggcgtt ggtgtaatgt 2100 ctccattcga ccaggcagtt ccatattact tctacagaat tgacaaaaca ggtgcatatt 2160 tgaacgaaag tggctgtggt aatgtcattg caagcgaaag accgatgatg agaaaataca 2220 tagtcgacac actcaaatgg tgggtcacgg aatacaaaat tgatggtttc agatttgacc 2280 agatgggatt aatggataag gtgacaatgc ttgcgataaa atcagaactc tcaaagatag 2340 agccttctgt ggttctttac ggtgaacctt ggggcggttg gggagcaccc attagatttg 2400 gaaaagccga tgttggtggt acaggtatcg ccgcgttcaa cgatgaattc agagatgcac 2460 tccgtggttc tgttttcaac gcaactgtca agggcttctt gatgggcgct cttgccaaag 2520 aaacaggtgt aaaacgcgga gttgcaggta gcatcgaata cgacgaagta attcgaagct 2580 ttgcgaaaga cccacaggaa actatcaact acgtcgaagt tcatgataac cacaccttgt 2640 gggacaaaaa ctacctggca gcacaggcgg atacaacggt aaagtggact gaagaaatgc 2700 tcaaagacgc tcaaaagctt gctggtgcta tattgttgac atcacaaggt ataccattct 2760 tgcatgcagg tcaagatttc gctagaacaa agaaattcga tgagaactct tacaaatcac 2820 caatctccat aaacggactt gactacgcta gaaaagccga gtttatagat gttttcaatt 2880 actacaaagg tctaatcgaa ctgagaaaat cacacattgc gtttaggcaa aggaccgccg 2940 aggatataag aaagaaactg acattcttac caagtccaag aaagatggta gcatttgtac 3000 ttaaagatga gaaagaccca tggaaggaaa tactcgtaat ctacaacggt gacacaaaag 3060 accaacaatt cacacttccg gatgggactt ggaacgtcgt tgttgacaaa gataacgcag 3120 gaacaaaggt tctctaccaa gttagcggta agataaatat taaagctata tcagctatgg 3180 taatgtacaa aggtaattaa cagaaacttg ttatcgatga gttgaaatat taacaattca 3240 tatggtataa tatccccgag aaagggggta agagtatgcc caaaagaatt ctcgtagttg 3300 aagatgaacc caacatgaga ttactaatag ccgaagaatt aatggacgct ggttatgaag 3360 tagatgaagc tgaaaatgct gacgaagcgc taaaaaaatt tggtgaaaaa cagtatgatc 3420 ttgttactat cgatatcgag atgcctggaa gcatgaacgg attggaactt gcgggtaaac 3480 tgagagaaat caggcgggaa acaaaattgg ttctattgac tgcttactct cattacaaaa 3540 gcgatatggc ttcttgggca gctgatgcct acattgtgaa gtctgctgat ttaactgaat 3600 taaaagaagt aatcagtcgt ttgattaaca tgtgaatggt gcccacacct ttcaagaaag 3660 gggtgggcat cacccctttt tttctattat aaaatagctg ataggataaa actgcattag 3720 gaggtgctca aattggacta taaagcaacg ctgaacttac cacaaacaaa ctttcaaatg 3780 aaggctaacc ttgtgaacaa ggaaccggag atgttgaaat tctgggaaga aaaggagatt 3840 tacaaaaaga ctttggaaac aagagcaaat gcaccaacat accttctaca cgatggacct 3900 ccgtacgcaa atggagacat ccatcttggt accgctatga ataagatact gaaagatttc 3960 gtcaccagat acaaaaccat gcgcggttac agagtgcctt tcgttcctgg atgggataca 4020 cacggattgc caattgaaca cagagttaca acatccttgg gagaggaagc aaagaaaaaa 4080 tcaccagccg aaataagaaa gctttgcaaa gagtttgctc tgaaatacgt agacattcag 4140 agagaggaat tcaaacgact cggcgtaaaa ggtgactggg aacatccata cataacactt 4200 gacccagatt acgaatacca tatactcgat gtatttaaaa cgttggtgga aaacggcaac 4260 gtctatcgtg gtaacaagcc tgtttattgg tgtccaacgt gtagaacagc ccttgcggaa 4320 gcagaaatcg aataccacga ccacgaatct ccatcaattt atgtaaaatt ccaaatggtc 4380 gataaaccag acacgtatat tgtaatctgg accacaaccc cttggactat cccagcaaat 4440 gttgctatcg cgctccatcc agattacaca tatgtcaaaa taaaagtcga cgaagaatac 4500 tggattgttg cagaaggcct acttcaaaag ttcgcggcag atgttggtat tgactttgaa 4560 gttgttgaaa aattcgtagg caaagaactt gaaggcaaac ttacaaaaca cccactttac 4620 gacagaactt ctgttgtcgt tcttgccgat tacgtaactc ttgaagatgg taccggttgt 4680 gtccatacag ctccgggaca cggtgaagaa gactatcaaa cgggtttaaa atacaatctt 4740 ccagttcttt cgcccgtgga tgacgaaggt agattcacaa aagaggcagg aaaatatgaa 4800 ggattaaaga tatgggatgc caacaaagtt atagttgaag acctaaaaaa caacggttct 4860 ttgattaaag cgggaaagat tagccatagc tacccacact gctggcgttg taaaggaccg 4920 gttatgttcc gtgccacacc acaatggttc atctccgttg ataaaaataa cttacgaggt 4980 aaagtgctcg aggagataaa gaaagttaaa tggtatcctg cttggggtga aacacgtatc 5040 accgcaatgg ttcaggaaag acctgactgg acgatttcac gtcaaagggt ttggggagta 5100 ccaattccag cggtaaaatg taaagactgt ggcgaagtta cactcgagcc aaaagtaatc 5160 gagcactttg caaatatagt aaaagaaaaa ggcacagatg cctggttcga gcttgacgtt 5220 aacgaactaa ttccagcaga tttcaaatgc ccagcttgcg gaagcaagaa ttttgaaaag 5280 acacatgata cattagatgt ttggatggat tccggatgtt catgggaagc ggtgatacgc 5340 tccaaaggtg agaggttccc agttgaccta tacttagaag gtgatgacca acacagaggt 5400 tggttccaaa gctccatatt cttatccact gcaaaagcgg gaactgcacc attcaaagct 5460 gttgtcacgc acggtttcat caaagatgaa caaggcagaa agatgagtaa atctctcgga 5520 aacgtaattg atccaatgga aattgtcaac aagtatggtg ctgacatatt gagattgtgg 5580 gttgcaagca cggacttctt tgataacatc agggttggta agaacataat agaacagcaa 5640 gttgaggtct acagaaagtt gagaaacacc ctgagatatt tgctcagtaa cctttatgac 5700 tttacagaag cagacttgct accatacgaa aaactgctcc cattggacaa atgggcactt 5760 ggcagacttc aaaaattcat cgaacagata acacaatact acgaaggatt cgaatactcc 5820 aaagtctaca acgcaactgt caaatactgc accacagaac taagtgctgt gtatttagat 5880 atactcaaag acagactcta cgtcgaggca aaagattcaa tctacagaag gtctgctcaa 5940 actgctctac actacatact tgaagcactg ataaagattc ttgcaccaat tattccattc 6000 acggcagaag aagcatacca agaaagccat ttgaagagat acgaaagcgt gcatcttgaa 6060 tactggccag aagtcagaaa agaatttatc gacgaagcgc ttcttgagga attcgatcat 6120 ttgctactca taagggacga tgtgctgaaa gcactggaaa acgccagggc atctgatatt 6180 atcggtcatt cactcgatgc ccatgtaata atcgaagcaa agaacgaaga attgaaaaac 6240 ttgctcagaa aatacgagtc actgcttgaa gaattcttca ttgtttcaaa agtgactttg 6300 tctgaaaaca tctctggatt aaacggtcag tttgcaaatg ttttagtcca aagagcagaa 6360 ggtcaaaaat gtcagcgctg ttggaaatac cacccagata ccggtaaaga cctagaacac 6420 ccagaaacat gtcctcgctg ttcagccgtg cttagagggg aaaggaaata aggaagaaag 6480 aataagaaga gatgataaga tggaaagagt tgcgagatat attcacaaag aaaagcattt 6540 tgaattatct gtaattttgc ctttgataat tttaattatc ccggcagtca tagcaactgc 6600 cggggtttta gtcccacaaa aattggggat taacaaaatt gaactgggaa aagaactcaa 6660 agttgatgga aaaaactttc ctttcacatt tctcgatgga atgctcttgg ctgatgggaa 6720 gagaactata accataaatg accaaaaata caccgttgaa gtaaaaaccg ctcccgacaa 6780 aattcgactg aatggaatta cggaagtcta tacgtatgtc tttgacagca cagtcacatt 6840 ttacgaccca acggtaagag ccctgaaagc cattgataaa tcaaacgaaa tcaaactttc 6900 tgacaaattc tatctggcag ttgcgagtga tgattttgat aatgtaacgg atgttgcttt 6960 tgttccgcca acacttgaga aacttgacat acttgattca caaaacccga ttacaaatat 7020 ttctggtagg aagttcctac tttcttcaaa atcaccttac aagataatca gcaaaaccat 7080 actacccgag aaaagtgctc ttatgctgga aggtggagtt acaatcatca ccgctctcaa 7140 tgccgaactg aatatcaagg gtgctcttgt aacaacagga ctagttagct tcttgggaag 7200 tggaaaactc acagtttccg ataacggttc agcttatgtt agtgccattg gtcccggaat 7260 tgatattacc tcagacaggg gtgcgctgat ttttgtagac ggttcaaatc ttcgaaacat 7320 tgatgccaat ttaactaact ttgtagtaat tagaaaatcc tcgcttagta aggtatcaat 7380 caacggtgtt tatacagttt atatcatcga ttcatcaatt gaagaacttg aaattcaaaa 7440 ttgtgggaat gttgtgataa acaacgcagc tgttaataac gtaaaagttg gattagtatc 7500 aaaagttatt tcgtacaact ctcggtctga caaattttcg gtctctgacc tttcatcggk 7560 tgctctggta tcctcaaaaa ttacagactt acagctgtct aaaggtagtg ttctgaaaat 7620 taaaaattcg tcgttaataa acgcaaaagt cgaaaactat tcaattgcct acctatttaa 7680 aaccaatgtc aaccaattaa gcgttggaaa cgcaaaatac tacttgttag aaagcaaaac 7740 aaacaagatt acaaaaattc aagaacaata acttttttag tagtttcgag gggggttgta 7800 tatggcagat tgtgtgttct gcaagattat aaacggagaa atctcttcag aaaaagtgta 7860 tgaagatgaa gatttcatag tcattaaaga cattagacct gttgcacctg tccacctact 7920 ggtgatttac aaaaagcatg tgccaacggt tagtgaactt tctgttgagg atagtcaaaa 7980 gatgtggaaa ttgttcgaag tcataaagac cgtaaccaaa acaagtggtt tggaaagcta 8040 cagaattgtt caaaacaacg gcaaagatgc aggacaagaa gtgcaccata ttcacttcca 8100 cataatcgga ggaagaaaat taggatcc 8128 // |
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