EBI Dbfetch

ID   AF082011; SV 1; linear; mRNA; STD; INV; 1982 BP.
XX
AC   AF082011;
XX
DT   17-AUG-1998 (Rel. 56, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   Caenorhabditis elegans UDP-N-acetylglucosamine:a-3-D-mannoside
DE   b-1,2-N-acetylglucosaminyltransferase I (gly-12) mRNA, complete cds.
XX
KW   .
XX
OS   Caenorhabditis elegans
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
XX
RN   [1]
RP   1-1982
RX   DOI; 10.1074/jbc.274.1.288
RX   PUBMED; 9867843.
RA   Chen S., Zhou S., Sarkar M., Spence A.M., Schachter H.;
RT   "Expression of three Caenorhabditis elegans N-acetylglucosaminyltransferase
RT   I genes during development";
RL   J. Biol. Chem. 274(1):288-297(1999).
XX
RN   [2]
RP   1-1982
RA   Chen S., Zhou S., Sarkar M., Spence A., Schachter H.;
RT   ;
RL   Submitted (30-JUL-1998) to the EMBL/GenBank/DDBJ databases.
RL   Structural Biology and Biochemistry, Hospital for Sick Children, 555
RL   University Avenue, Toronto, Ont M5G 1X8, Canada
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1982
FT                   /organism="Caenorhabditis elegans"
FT                   /chromosome="X"
FT                   /mol_type="mRNA"
FT                   /clone_lib="provided by S. Kim and R. Horvitz, MIT"
FT                   /note="library screened with probes based on the
FT                   Caenorhabditis elegans cosmid F48E3 sequence in GenBank
FT                   Accession Number U28735"
FT                   /db_xref="taxon:6239"
FT   misc_RNA        1..22
FT                   /gene="gly-12"
FT                   /product="SL1 trans-spliced leader sequence"
FT   CDS             309..1712
FT                   /codon_start=1
FT                   /gene="gly-12"
FT                   /product="UDP-N-acetylglucosamine:a-3-D-mannoside
FT                   b-1,2-N-acetylglucosaminyltransferase I"
FT                   /function="catalyzes first step in complex N-glycan
FT                   synthesis"
FT                   /EC_number="2.4.1.101"
FT                   /note="glycosyltransferase; F48E3.1; GlcNAc-transferase I;
FT                   active on expression"
FT                   /db_xref="GOA:Q9GPA3"
FT                   /db_xref="HSSP:1FO9"
FT                   /db_xref="InterPro:IPR004139"
FT                   /db_xref="UniProtKB/TrEMBL:Q9GPA3"
FT                   /protein_id="AAD03023.1"
FT                   /translation="MKRVLRRSASKHASTLFKIICILVLCSFIIYLKSEDHDLQKNTAI
FT                   HAPRSNSREDEDDILIPADNKDQQFVAAVLVFCATRPDALRNHLSQILAQRPSHFQYHI
FT                   IVSQDGNKTAVTQVAQKFVKDYKNVSHIQHEKTEIKKRNNYPAISAHYKWALDKAFKGF
FT                   RYDHVIVTEDDLDIGNDFFSYFRWGKQVLNSDDTIWCVSAWNDNGGGPLIDSTRGDLIW
FT                   RTDFFPGLGWMLTKKLWNELSPGFPVAYWDDWMRKPEVRKSRSCIRPEISRTSHNMKLA
FT                   GKGSSGGMFKDYLSKISASSANIDFSLLPVTLVQKSIYDKRLIEQIENARPIDLQNTTG
FT                   MEKTYNYKIVYKNIRDWHRLAAHFKLMTDIRGGMQRTAYYGVVTLMFQNCRVFLVPEST
FT                   YRNPSQLTSYVYDSEWDKQNRFIEFEAYYCKTKKYAGKCDPHSPEMIAFFKKKGWKKRL
FT                   DDWGEMLVV"
XX
SQ   Sequence 1982 BP; 582 A; 449 C; 414 G; 537 T; 0 other;
     atcgcagctg cctctatcgt ccgcgtcacc cacaaatacg aagagactgg acaacgctag        60
     ccgcattctt ctttggtgag agatttagac acaattcggg gccgaaccag cgagcgctag       120
     ttcggccatt tcggccacgc cgaaaccaag cgaagacgac tcgcacgctc tcatctcttc       180
     ctcttcccgt cacctcgttt tccattcata gtctactcgt gtcgttattg ggacgcttct       240
     agaatatttg agcggatcga caacaacgtt tcaacacttg tccttctttc caaggaccac       300
     caacgcccat gaaacgggtg ctccgtcgct cggcatcaaa acacgcgagc acactgttta       360
     aaattatctg tattcttgta ttatgctctt ttattattta cctgaagtct gaagaccatg       420
     atttgcaaaa aaatactgct attcacgctc cacggtccaa ttcgcgggaa gatgaggatg       480
     atattttaat accggctgat aataaagatc aacaatttgt tgcagctgta cttgtttttt       540
     gcgcaaccag gcccgatgct cttcggaatc atttatcaca aattttagcc caacgccctt       600
     cacactttca ataccacatc attgttagtc aagatggcaa taagaccgcc gtcacacaag       660
     tcgcacaaaa gtttgtcaaa gactacaaaa acgtgtcaca tatacaacat gagaagacgg       720
     aaatcaaaaa gcggaacaac tacccagcca tctcggcaca ttacaaatgg gccttggaca       780
     aagccttcaa aggcttccgt tatgatcatg tcattgtgac tgaagacgac cttgacatag       840
     gtaacgattt cttttcctac ttccgatggg gaaaacaagt tctcaactct gatgacacta       900
     tttggtgtgt atctgcgtgg aatgacaatg gaggaggacc acttatagat tcgacgcgag       960
     gcgatttaat ttggaggacg gacttctttc cgggattagg atggatgctc acaaaaaaac      1020
     tctggaacga actttcgccc ggatttcctg ttgcgtattg ggatgactgg atgcgaaagc      1080
     cggaagtgcg gaagagcagg tcatgtatcc gtccggaaat cagtagaacg tcgcataaca      1140
     tgaagttagc tggaaaaggg tctagtggag gtatgttcaa agactatctc agcaaaatta      1200
     gcgcatccag cgcaaacatt gacttctctc ttctccctgt caccctcgtc caaaaatcaa      1260
     tctacgacaa gcgccttata gaacaaattg agaatgcacg acccatagac ctgcagaaca      1320
     ctactgggat ggagaaaacc tacaattaca aaattgtgta caagaacatt cgagactggc      1380
     ataggctggc cgcccatttc aaattgatga cagacattcg aggaggaatg cagcgaacag      1440
     catactatgg agttgtcact ttgatgttcc agaattgcag agtgtttttg gtacctgaat      1500
     caacgtaccg aaatccatca caacttacta gttacgtcta cgattcagaa tgggacaaac      1560
     aaaaccgatt tattgaattt gaagcatatt actgcaaaac taaaaaatat gctggaaaat      1620
     gcgacccaca cagtcccgag atgattgcct ttttcaaaaa gaaaggatgg aagaaacgat      1680
     tggatgattg gggcgagatg ctagttgttt agtgaacaag acgaggtaca actgtcctgt      1740
     gacgatacgg gtgattgtaa gcgttctttc gaaagaacat ttgtcggacc tgttcagtta      1800
     tatgcgtgca tgtcagatca atcaactcgt caaacttgct gctgtaacta tgatttttgc      1860
     aacggttctt caaataacaa tagtatttat ttaattatta tgctggctgt cattataatt      1920
     tattcattcc aatgctctct tcagtttcca acttttggga ttctttccgt ttagctttcg      1980
     tt                                                                     1982
//