EBI Dbfetch

ID   AF080548; SV 1; linear; genomic DNA; STD; PRO; 1478 BP.
XX
AC   AF080548;
XX
DT   13-AUG-1998 (Rel. 56, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   Sinorhizobium meliloti megaplasmid pRmeSU47b glycerol kinase (glpK) gene,
DE   partial cds; 3-hydroxybutyrate dehydrogenase (bdhA) gene, complete cds; and
DE   xanthine dehydrogenase/oxidase (xdhA) gene, partial cds.
XX
KW   .
XX
OS   Sinorhizobium meliloti
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae;
OC   Sinorhizobium/Ensifer group; Sinorhizobium.
OG   megaplasmid pRmeSU47b
XX
RN   [1]
RP   1-1478
RX   PUBMED; 9922248.
RA   Aneja P., Charles T.C.;
RT   "Poly-3-hydroxybutyrate degradation in Rhizobium (Sinorhizobium) meliloti:
RT   isolation and characterization of a gene encoding 3-hydroxybutyrate
RT   dehydrogenase";
RL   J. Bacteriol. 181(3):849-857(1999).
XX
RN   [2]
RP   1-1478
RA   Aneja P., Charles T.C.;
RT   ;
RL   Submitted (27-JUL-1998) to the EMBL/GenBank/DDBJ databases.
RL   Biology, University of Waterloo, 200 University Avenue West, Waterloo,
RL   Ontario N2L 3G1, Canada
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1478
FT                   /organism="Sinorhizobium meliloti"
FT                   /plasmid="megaplasmid pRmeSU47b"
FT                   /map="at 200 kb on transductional linkage map"
FT                   /strain="SU47; Rm1021"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:382"
FT   CDS             complement(<1..179)
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="glpK"
FT                   /product="glycerol kinase"
FT                   /EC_number="2.7.1.30"
FT                   /note="GlpK"
FT                   /db_xref="GOA:O86033"
FT                   /db_xref="HSSP:1GLC"
FT                   /db_xref="InterPro:IPR005999"
FT                   /db_xref="UniProtKB/Swiss-Prot:O86033"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAD12735.1"
FT                   /translation="MGGYILAIDQGTTSTRAIVFDGNQKIAGVGQKEFKQHFPKSGWVE
FT                   HDPEEIWQTVVSTV"
FT   RBS             complement(186..190)
FT                   /gene="glpK"
FT                   /inference="non-experimental evidence, no additional
FT                   details recorded"
FT   misc_feature    330
FT                   /gene="bdhA"
FT                   /note="transcriptional start site, S2"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   protein_bind    365..378
FT                   /gene="bdhA"
FT                   /bound_moiety="RpoN (sigma 54)"
FT                   /note="putative binding site"
FT   misc_feature    432..433
FT                   /gene="bdhA"
FT                   /note="transcriptional start site, S1"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   RBS             461..464
FT                   /gene="bdhA"
FT                   /note="RBS-1"
FT                   /inference="non-experimental evidence, no additional
FT                   details recorded"
FT   RBS             466..469
FT                   /gene="bdhA"
FT                   /note="RBS-2"
FT   CDS             479..1255
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="bdhA"
FT                   /product="3-hydroxybutyrate dehydrogenase"
FT                   /EC_number="1.1.1.30"
FT                   /note="BdhA"
FT                   /db_xref="GOA:O86034"
FT                   /db_xref="HSSP:1NFF"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:O86034"
FT                   /protein_id="AAD12733.1"
FT                   /translation="MTKTAVITGSTSGIGLAIARTLAKAGANIVLNGFGAPDEIRTVTD
FT                   EVAGLSSGTVLHHPADMTKPSEIADMMAMVADRFGGADILVNNAGVQFVEKIEDFPVEQ
FT                   WDRIIAVNLSSSFHTIRGAIPPMKKKGWGRIINIASAHGLVASPFKSAYVAAKHGIMGL
FT                   TKTVALEVAESGVTVNSICPGYVLTPLVEKQIPDQARTRGITEEQVINEVMLKGQPTKK
FT                   FITVEQVASLALYLAGDDAAQITGTHVSMDGGWTAQ"
FT   RBS             1288..1292
FT                   /gene="xdhA"
FT   CDS             1299..>1478
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="xdhA"
FT                   /product="xanthine dehydrogenase/oxidase"
FT                   /EC_number="1.1.1.204"
FT                   /EC_number="1.1.3.22"
FT                   /note="XdhA"
FT                   /db_xref="GOA:O86035"
FT                   /db_xref="HSSP:1JRO"
FT                   /db_xref="InterPro:IPR012675"
FT                   /db_xref="UniProtKB/TrEMBL:O86035"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAD12734.1"
FT                   /translation="MAQASDSIRFILNDSEIVLSDVAPTSTLLDFLRLERRLTGTKEGC
FT                   AEGDCGACTVLVGRL"
XX
SQ   Sequence 1478 BP; 306 A; 457 C; 443 G; 272 T; 0 other;
     tggaccgtgc tgacgaccgt ctgccagatc tcctccggat cgtgctccac ccagcctgac        60
     ttcggaaagt gctgcttgaa ctctttctgc ccgacgcccg cgattttctg gttgccgtcg       120
     aaaacgatcg cgcgcgtgga cgtcgtgccc tgatcgatcg cgagaatata tccgcccata       180
     tgcccctcct cggcacgcga ttgaagggac cgaccggaat gcgagcggaa gcccgcatac       240
     gctttcctca ctccgccccc ctcgcccttc ctcgagggcg acattcgaac gacttcaata       300
     cgatagaaaa acgaatgtca aacgaaaaca aaacgcaatt ttcgccaggt gctcccgcgg       360
     acacggactc tgctttgccg ggcggaaaaa cacaattgtc actgccgcgc atttgcgcgt       420
     aggctgacgc tgttgcgtcg caaaagacct gtgcggcatc aggacggaga gagcgggaat       480
     gaccaagact gcggtgataa cgggttccac gagcggcatc ggattggcga tcgcccggac       540
     cctggcgaag gccggtgcca atatcgtcct gaacggcttc ggtgcgccgg acgagatcag       600
     gaccgtcacg gatgaagtcg caggcctgag ctccggtacg gtgcttcatc acccggccga       660
     catgaccaag ccctccgaaa tcgccgacat gatggcgatg gttgccgatc gcttcggcgg       720
     cgccgatatc ctcgtcaaca atgccggcgt gcagttcgtt gaaaagatcg aggattttcc       780
     ggtcgagcaa tgggaccgga tcatcgccgt caatctctcc tcctcctttc acaccattcg       840
     tggcgccatt ccgccgatga agaagaaggg ctggggccgg atcatcaata tcgcgtccgc       900
     tcatggcctc gtggcctccc ccttcaagtc cgcctatgtc gccgccaagc atggtatcat       960
     ggggttgacg aagactgtgg cgctggaggt ggcggagagc ggtgtcaccg tgaactcgat      1020
     ctgccccggc tacgttctga cgccgctcgt cgaaaagcag ataccggatc aggcgagaac      1080
     gcgcggcatc accgaggaac aggtgatcaa cgaggtgatg ctcaagggac agccgacgaa      1140
     aaagttcatc accgtcgaac aggttgcctc cctggcgctc tatcttgcag gcgacgatgc      1200
     cgcccagatc accgggacgc atgtttcgat ggatggcggc tggacggcgc agtagccgac      1260
     gactgaggga aaagccggtt gagggacaag gaagtggcat ggcacaggca tccgacagca      1320
     tccgcttcat cctgaacgac tccgagatcg tcctttccga cgtcgcgccg acgtcgacgc      1380
     tgctcgattt cctgaggctg gagcgccgcc tcaccggcac caaggaaggc tgcgcggaag      1440
     gcgactgcgg cgcctgcacg gtgctcgtgg gccggctc                              1478
//