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EBI DbfetchID AF075709; SV 2; linear; genomic DNA; STD; PRO; 5908 BP. XX AC AF075709; XX DT 17-AUG-1998 (Rel. 56, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Pseudomonas putida LsfA (lsfA), complete cds; and ssu locus, complete DE sequence. XX KW . XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-5908 RX DOI; 10.1046/j.1365-2958.1999.01398.x RX PUBMED; 10361295. RA Vermeij P., Wietek C., Kahnert A., Wuest T., Kertesz M.A.; RT "Genetic organization of sulphur-controlled aryl desulphonation in RT Pseudomonas putida S-313"; RL Mol. Microbiol. 32(5):913-926(1999). XX RN [2] RP 1-5908 RA Vermeij P., Leisinger T., Kertesz M.A.; RT ; RL Submitted (01-JUL-1998) to the EMBL/GenBank/DDBJ databases. RL Microbiology, ETH Zurich, Schmelzbergstrasse 7, Zurich CH-8092, Switzerland XX RN [3] RC Sequence update by submitter RP 1-5908 RA Kertesz M.A.; RT ; RL Submitted (05-MAY-2004) to the EMBL/GenBank/DDBJ databases. RL Microbiology, ETH Zurich, Schmelzbergstrasse 7, Zurich CH-8092, Switzerland XX DR CABRI; DSM 6884. DR StrainInfo; 160378; DSM 6884. XX CC On May 5, 2004 this sequence version replaced gi:3420602. XX FH Key Location/Qualifiers FH FT source 1..5908 FT /organism="Pseudomonas putida" FT /strain="S-313; DSM 6884" FT /mol_type="genomic DNA" FT /db_xref="taxon:303" FT gene 47..781 FT /gene="lsfA" FT -10_signal 47..52 FT /gene="lsfA" FT RBS 129..134 FT /gene="lsfA" FT CDS 143..781 FT /codon_start=1 FT /transl_table=11 FT /gene="lsfA" FT /product="LsfA" FT /db_xref="GOA:O85761" FT /db_xref="HSSP:1PRX" FT /db_xref="InterPro:IPR000866" FT /db_xref="InterPro:IPR012335" FT /db_xref="InterPro:IPR012336" FT /db_xref="InterPro:IPR017936" FT /db_xref="InterPro:IPR019479" FT /db_xref="UniProtKB/TrEMBL:O85761" FT /protein_id="AAC31902.1" FT /translation="MSLKLGDIAPDFEQDSSEGKIRFHEWLGNSWGVLFSHPADFTPVC FT TTELGLTAKLKDDFAKRGVKPIALSVDPVDSHHRWIEDINETQNTVVNFPIIADADRKV FT SELYDLIHPNANDTLTVRSLFVIDPNKKVRLTITYPASTGRNFNEILRVIDSLQLTDNH FT KVATPGNWQDGDDVVIVPSLKDEEEIKQRFPKGYRAVKPYLRLTPQLNR" FT gene 965..1608 FT /gene="ssuE" FT -10_signal 965..970 FT /gene="ssuE" FT RBS 1002..1007 FT /gene="ssuE" FT CDS 1015..1608 FT /codon_start=1 FT /transl_table=11 FT /gene="ssuE" FT /product="NADH-dependent FMN reductase" FT /note="SsuE" FT /db_xref="GOA:O85762" FT /db_xref="InterPro:IPR005025" FT /db_xref="InterPro:IPR020048" FT /db_xref="UniProtKB/Swiss-Prot:O85762" FT /protein_id="AAC31903.2" FT /translation="MLVVSIGGSPSTRSRSGVLLERSRQWLQDRGVEVVSFQVRDFPAE FT DLLHARFDSPQVQHFQQLVAQADGLVVATPVYKASFAGALKTLLDLLPERALEHKIVLP FT IATGGSIAHMLAVDYALKPVLSALKAQETLQGIFADDSQIAYGEGAKPAQLAPALEERL FT HDSLETFHVALARRPRPVAPGVLNERLISARWSI" FT gene 1683..2659 FT /gene="ssuA" FT RBS 1683..1688 FT /gene="ssuA" FT CDS 1694..2659 FT /codon_start=1 FT /transl_table=11 FT /gene="ssuA" FT /product="putative sulfonate binding protein precursor" FT /note="SsuA" FT /db_xref="GOA:O85763" FT /db_xref="InterPro:IPR001638" FT /db_xref="InterPro:IPR010067" FT /db_xref="InterPro:IPR015168" FT /db_xref="UniProtKB/TrEMBL:O85763" FT /protein_id="AAC31904.1" FT /translation="MRTVFLRRGLVALFAAAVSFGAITQAQAESLRIGYQKYGTLVLLK FT AKGTLEKRLAEQGVQVQWTEFPGGPQLLEGLNVGSIDFGVTGETPPVFAQAAGADLLYV FT AYEPPAPHSEAILVPKGSPIQSVKELKGKKVALNKGSNVHYLLVRALEDAGLKYSDIQP FT VYLPPSDARAAFERGSVDAWVIWDPYQAAAEQQLQARTLRDGQGLVDNHQFYLATRNYA FT TQHPAVISALIEEVRAVGEWSQANPQQVTDQVAPLLGLPADITLTSVKRQGYGAAPLTP FT EVVAAQQKIADTFQALKLIPKPLSIKDVIWTPPAKVASAP" FT sig_peptide 1694..1777 FT /gene="ssuA" FT mat_peptide 1778..2656 FT /gene="ssuA" FT /product="putative sulfonate binding protein" FT gene 2708..3868 FT /gene="ssuD" FT RBS 2708..2711 FT /gene="ssuD" FT CDS 2720..3868 FT /codon_start=1 FT /transl_table=11 FT /gene="ssuD" FT /product="putative FMNH2-dependent monooxygenase" FT /note="SsuD" FT /db_xref="GOA:O85764" FT /db_xref="InterPro:IPR011251" FT /db_xref="InterPro:IPR016048" FT /db_xref="InterPro:IPR019911" FT /db_xref="UniProtKB/Swiss-Prot:O85764" FT /protein_id="AAC31905.1" FT /translation="MSLNIFWFLPTHGDGKYLGTSDGARAVDHGYLQQIAQAADRLGFG FT GVLIPTGRSCEDSWLVAASLIPVTERLKFLVALRPGIISPTVAARQAATLDRLSNGRAL FT FNLVTGGDPDELAGDGLHLNHQERYEASVEFTRIWRKVLEGENVDYDGKHIQVKGAKLL FT YPPVQQPRPPLYFGGSSEAAQDLAAEQVELYLTWGEPPAAVAEKIAQVREKAAAQGREV FT RFGIRLHVIVRETNEEAWAAADRLISHLDDDTIARAQASLARFDSVGQQRMAALHGGNR FT DNLEVSPNLWAGVGLVRGGAGTALVGDGPTVAARVKEYADLGIDTFIFSGYPHLEESYR FT VAELLFPHIDVQRPEQPKTGGYVSPFGEMVANDILPKSVSQS" FT gene 3867..4676 FT /gene="ssuC" FT RBS 3867..3870 FT /gene="ssuC" FT CDS 3879..4676 FT /codon_start=1 FT /transl_table=11 FT /gene="ssuC" FT /product="ABC transporter membrane subunit" FT /note="SsuC" FT /db_xref="GOA:O85765" FT /db_xref="InterPro:IPR000515" FT /db_xref="UniProtKB/TrEMBL:O85765" FT /protein_id="AAC31906.1" FT /translation="MSRATSSTLTQRLAPWALPVLLLAAWQLAVSAGWLSTRILPAPSA FT VVSAGVELVRSGEIWTHLAISGWRAGLGFLIGGSIGLVLGFITGLSNWGERLLDSSVQM FT IRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYLNTYHGIRNVDPALVEMARSYG FT LSGFGLFRQVILPGALPSILVGVRFALGFMWLTLIVAETISANAGIGYLAMNAREFLQT FT DVVVLAIVLYAVLGKLADLAARGLERVWLRWHPAYQVAKKEGA" FT sig_peptide 3879..3965 FT /gene="ssuC" FT gene 4663..5485 FT /gene="ssuB" FT RBS 4663..4668 FT /gene="ssuB" FT CDS 4673..5485 FT /codon_start=1 FT /transl_table=11 FT /gene="ssuB" FT /product="ABC transporter ATP-binding subunit" FT /note="SsuB" FT /db_xref="GOA:Q8KZQ6" FT /db_xref="InterPro:IPR003439" FT /db_xref="InterPro:IPR003593" FT /db_xref="InterPro:IPR017871" FT /db_xref="InterPro:IPR017875" FT /db_xref="UniProtKB/Swiss-Prot:Q8KZQ6" FT /protein_id="AAC31907.1" FT /translation="MTVLKEQPPRLLRGIPLASNGLRKTFGQREVLRGIDLHIPAGQFV FT AIVGRSGCGKSTLLRLLAGLDQPTAGELLAGAAPLAEAREETRLMFQDARLLPWKKVID FT NVGLGLKGDWRQRALDALEAVGLADRANEWPAALSGGQKQRVALARALIHQPRLLLLDE FT PLGALDALTRIEMQQLIERLWRQHGFTVLLVTHDVNEAVAVADRVILIEDGEVGLDLNV FT DLARPRARGSHRLAALESEVLNRVLSVPGTPPEPEPVAPLPTQLRWAH" FT gene 5505..5732 FT /gene="ssuF" FT RBS 5505..5509 FT /gene="ssuF" FT CDS 5517..5732 FT /codon_start=1 FT /transl_table=11 FT /gene="ssuF" FT /product="putative molybdopterin-binding protein" FT /note="SsuF" FT /db_xref="GOA:O85767" FT /db_xref="HSSP:1GUG" FT /db_xref="InterPro:IPR004606" FT /db_xref="InterPro:IPR005116" FT /db_xref="InterPro:IPR008995" FT /db_xref="UniProtKB/TrEMBL:O85767" FT /protein_id="AAC31908.1" FT /translation="MTIKAINVRNQFKGTVKEILEGPVLSEIDVQTASGIVTSVITTRS FT VKELELQVGSEVIAFVKSTEVSIAKL" XX SQ Sequence 5908 BP; 1099 A; 1938 C; 1795 G; 1076 T; 0 other; gcctggtgcg aggctttttt atgcctgcaa tctcttgttc cttatggtta taaataaatc 60 agtatttatt ctttttattt ttaatcagat gcaggcacat tgaacctcac aaggccagaa 120 cacagcacag gagccgcacc ccatgagcct caaactcggc gatatcgccc ccgacttcga 180 acaggattcc agcgaaggca agatccgctt ccacgaatgg ctcggcaaca gctggggtgt 240 gctgttctcc cacccggccg acttcacgcc ggtgtgcacc actgagctgg gcctgaccgc 300 caagctcaag gatgacttcg ccaagcgcgg ggtcaagccc attgccctgt cggtggaccc 360 ggtcgactcg caccatcgct ggatcgaaga catcaacgag acccagaaca ccgtggtcaa 420 cttcccgatc atcgccgacg ccgaccgcaa ggtgtccgag ctgtacgatc tgatccaccc 480 caacgccaac gacacgctga ctgtgcgttc gctgttcgtc atcgacccca acaagaaggt 540 gcgcctgacc attacctatc cggccagtac cgggcgcaac ttcaacgaga tcctgcgggt 600 gatcgactcg ctgcagctca ctgacaacca caaggtcgcc acaccaggta actggcagga 660 cggcgacgac gtggtgatcg tgccttcgct caaggacgaa gaagagatca agcagcgctt 720 ccccaaaggt tatcgcgctg tgaaaccgta tctgcggctg accccgcaac ttaaccgtta 780 acgcattctt cgttgcattg ttcttagcca tagcagggat tttcgggccg tttcgacggc 840 ccttttttat gcctgcacgc tttagtccgc agccggtgga agaggccggc ttttacgtga 900 ggcgtgctat ctgtcgataa aagcaaaatg gaataaacaa atgaaaaaat atgatttcta 960 gatatataac cgggctggta atgtcacccc aacaaaacac aaggaagcgc tccaatgctg 1020 gtcgtctcaa tcggtggtag ccccagtacc cgttcacgct ccggcgtgct gctcgagcgc 1080 tcgcgccagt ggctgcagga ccgtggcgta gaggtggtga gcttccaggt gcgtgacttt 1140 ccagccgagg acttgctcca tgcgcgcttc gacagcccgc aggtgcagca cttccagcaa 1200 ctggtagccc aggccgacgg cctggtggtg gcaaccccgg tatacaaggc gtcgttcgcc 1260 ggagccctga agacgttgct cgacctgctg cccgagcgcg cactggagca caagatcgtg 1320 ctgcccatcg ccaccggtgg cagcatcgcc cacatgctgg cggtggatta cgcgttgaag 1380 ccggtgctgt cggcactcaa ggcccaagag accctgcaag ggatcttcgc cgacgacagc 1440 cagatcgcct acggagaagg cgcgaagcca gcccagctgg cacccgctct ggaagagcgc 1500 ttgcacgatt cgctggaaac attccacgtc gccctggccc gtcggccgcg ccccgtggca 1560 cctggtgtac tcaatgaacg cctgatcagc gcccgctgga gcatctgacc cggccttacc 1620 gctgtaccac cttactcgcc cgtcaacgag gcaagcaggt gcaacccgaa ccccattcgc 1680 acaggagagc gccatgcgca cagtcttctt gcgtcgcggt ctggtcgccc tgtttgcggc 1740 ggctgtatct ttcggcgcca ttacccaagc ccaggcggaa agcctgcgta tcggttatca 1800 gaaatacggc accctggtgc tgctcaaggc caagggtacg ctggaaaagc gcctggccga 1860 gcagggtgta caggtgcagt ggaccgagtt tcccggcggc ccgcagttgc ttgaaggctt 1920 gaacgtcggc tcgatcgact ttggcgttac cggcgagaca ccccctgtat ttgcccaggc 1980 ggccggcgcc gatctgctct acgtcgccta cgagccacct gcgccccaca gcgaagccat 2040 cctggtgccc aagggttcgc cgattcagtc ggtgaaggaa ctcaaaggca agaaggtcgc 2100 cttgaacaaa ggttccaacg tgcactacct gctggtgcgc gcgctggaag acgctggcct 2160 gaaatacagc gacatccagc ctgtctacct gccaccatcc gatgcccgcg ccgccttcga 2220 gcgtggcagc gtcgatgcct gggtcatctg ggacccgtac caggcggctg ccgagcagca 2280 gctgcaggca cgcaccctgc gtgacggcca gggcctggtc gacaaccatc agttctacct 2340 ggccacgcgc aactacgcca cccagcaccc tgcggtgatc agcgccctga tcgaagaagt 2400 ccgtgctgtg ggtgaatggt cccaggccaa cccgcaacaa gtgaccgacc aggttgcgcc 2460 gctgctgggc ctgcccgccg acatcaccct gacctcggtc aagcgccaag gctacggagc 2520 cgcgccgctg acgcccgagg tggtagccgc gcaacagaaa atcgccgaca ccttccaggc 2580 gctcaagctg atacccaagc cgctgagcat caaggacgtg atctggacac ccccagccaa 2640 ggtcgccagc gcgccttgat cgattcgcct gcgccggggc gccgctccgg cttgagccac 2700 ccttgaggag acaactccaa tgagccttaa tattttctgg ttcctcccta cccacggtga 2760 cggcaagtac ctgggcactt ccgacggcgc ccgcgcagtc gatcacggct acctgcagca 2820 gattgcccag gctgccgacc gccttggttt tggcggtgtg ctgatcccca ccgggcgctc 2880 ctgcgaggac tcctggctgg tggccgcgtc gctgatcccg gttaccgaac gcctgaagtt 2940 cctggtggcc ctgcgcccag gcatcatttc gccaaccgtg gctgcgcgcc aggccgccac 3000 gctggaccgc ctgtccaacg gccgtgcgct gttcaacctg gtgaccggcg gtgacccgga 3060 cgaactggcc ggcgacggcc tgcacctgaa ccaccaggag cgctacgaag cctcggtcga 3120 gttcacccgc atctggcgca aggtgctgga aggcgaaaac gtcgattacg acggcaagca 3180 cattcaggtc aaaggcgcca agctgctgta tccgccggtc cagcaaccac gtccgccgct 3240 gtatttcggc ggctcgtccg aagccgccca ggacctcgcc gccgagcagg tcgagctgta 3300 cctgacctgg ggcgagccac cggcagccgt tgccgaaaag atcgcccagg tgcgcgagaa 3360 ggccgctgcc caaggccggg aagtacgctt tggtatccgc ctgcatgtga tcgtgcggga 3420 aaccaacgaa gaggcctggg ctgccgctga ccgcctgatc tcgcacctgg acgatgacac 3480 catcgcccgt gcccaggcgt cgctggcacg cttcgattcg gtgggccagc agcgcatggc 3540 tgccctgcat ggtggcaacc gcgacaacct ggaagtcagc cccaacctgt gggcgggtgt 3600 cggcctggtg cgtggtggtg ccggtaccgc gctggtcggc gatggcccga ccgtcgcggc 3660 ccgggtcaag gagtacgcgg acctgggtat cgataccttc atcttctcgg gttacccaca 3720 cctggaagag tcctaccgcg tggccgagct gctgttcccg cacatcgatg tgcagcgccc 3780 ggagcaaccg aagaccggcg gctatgtgag ccccttcggc gagatggtag ccaacgacat 3840 cctgcccaag tccgtgtcgc agagctgagg gccgggtcat gagtcgtgca acttcctcca 3900 ccctgaccca gcgcctggcg ccgtgggcgc tgccagtcct gctgctggca gcctggcaac 3960 tggcagtcag cgctggctgg ctttcgaccc gcatcctgcc ggcacctagc gcggtggtca 4020 gtgccggtgt agagctggtg cgcagtggtg aaatctggac ccacctggcc atcagtggct 4080 ggcgtgccgg cctgggcttt ctgatcggcg gcagcatcgg cctggtgctg ggcttcatca 4140 ccggcctgtc gaactggggc gaacgcctgc tcgacagctc ggtgcagatg atccgcaacg 4200 tgcctcacct ggcgctgatc ccactggtga tcctgtggtt cggtatcgac gagtcggcga 4260 agatcttcct ggtcgcgctg ggcacgctgt tcccgatcta cctgaacacc taccacggta 4320 tccgcaacgt cgacccggcg ctggtggaga tggcgcgtag ctatggcctg tccggtttcg 4380 gcctgttccg ccaggtgatc ctgccgggtg cactgccttc gatcctggtc ggtgtgcgct 4440 tcgccctggg cttcatgtgg ctgaccctga tcgttgccga gaccatttcg gccaacgccg 4500 gcatcggcta cctggcgatg aatgcccgtg aattcctaca aaccgacgtg gtggtactgg 4560 ccatcgtcct gtatgccgtg ctcggcaagc tcgccgacct cgccgcccgc ggcctggaac 4620 gtgtctggtt gcgctggcac ccggcctacc aagtggccaa gaaggagggc gcatgaccgt 4680 gctcaaggaa caaccaccac gcctgttacg cggcatcccg ctggcctcca atggcctgcg 4740 caagaccttt ggccagcgcg aagtgctgcg cggtatcgac ctgcacattc cggccggcca 4800 gtttgtcgcc atcgttggcc gcagcggctg tggcaagagc accttgctgc gcctgctggc 4860 cgggctcgac caacccacgg ccggtgaatt gctggccggt gccgcgccgc tggccgaggc 4920 gcgggaagaa acccgcctga tgttccagga tgcgcgcttg ctgccctgga aaaaggtgat 4980 cgacaacgtc ggcctgggcc tcaaaggcga ctggcgccag cgggcgctgg atgccctgga 5040 agcggttggc ctggccgacc gggccaatga atggccggct gcgttgtccg gtggccagaa 5100 gcagcgtgtc gccctggccc gggcattgat ccaccagccg cgcttgctgc tgctggatga 5160 accgctcggc gccctggacg ccctgacccg tatcgagatg cagcagctga tcgaacgcct 5220 gtggcgtcag catggtttca ccgtgctgct ggtcacgcac gacgtcaatg aagccgtcgc 5280 cgtggccgac cgggtgatcc tgatcgagga cggcgaagtc ggcctggacc tcaacgttga 5340 cctggcgcga ccaagggcgc gtggttcgca ccgcctggcc gcgctggaaa gcgaagtact 5400 caaccgtgtt ctgtcggtcc cgggcacccc gcccgaaccg gagcctgtag cccctctgcc 5460 cacgcaattg cgttgggcgc actgaacccc agaagccaaa gagaaggaat caagtcatga 5520 ctatcaaagc gatcaacgtc cgcaaccagt tcaaaggcac tgtgaaagaa atcctcgaag 5580 gcccggtact gtcggaaatc gacgtacaga ccgcctccgg tatcgtcact tcggtgatta 5640 ccacgcgctc ggtaaaagag ctggagctgc aggtgggcag tgaagtgatt gccttcgtga 5700 agtcgaccga ggtgtccatc gccaagctgt gatggcaatg gcggcgcctg gcaacccaac 5760 cgcgggcctt gaccgcgatt gggccgccag gcggccctac ccttaagcgc taagcgggtc 5820 gcagtccgcc aaagaggctg ttggcttggc tttgcgtaag ggcgccgcgc caggcgcccg 5880 cgcccaggtt gcgttcgtgg gcaagctt 5908 // |
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