 |
|
EBI DbfetchID AF053723; SV 1; linear; genomic DNA; STD; PRO; 5090 BP. XX AC AF053723; XX DT 03-APR-1998 (Rel. 55, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Actinobacillus pleuropneumoniae putative glycosyltransferase (cps5A), DE region 2 capsular polysaccharide biosynthesis protein (cps5B), region 2 DE capsular polysaccharide biosynthesis protein (cps5C), and DE 3-deoxy-D-manno-2-octulosonic acid 8-phosphate synthetase (cps5D) genes, DE complete cds. XX KW . XX OS Actinobacillus pleuropneumoniae OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Actinobacillus. XX RN [1] RP 1-5090 RX PUBMED; 9632602. RA Ward C.K., Lawrence M.L., Veit H.P., Inzana T.J.; RT "Cloning and mutagenesis of a serotype-specific DNA region involved in RT encapsulation and virulence of Actinobacillus pleuropneumoniae serotype 5a: RT concomitant expression of serotype 5a and 1 capsular polysaccharides in RT recombinant A. pleuropneumoniae serotype 1"; RL Infect. Immun. 66(7):3326-3336(1998). XX RN [2] RP 1-5090 RA Ward C.K., Lawrence M.L., Inzana T.J.; RT ; RL Submitted (13-MAR-1998) to the EMBL/GenBank/DDBJ databases. RL Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, RL 1410 Price's Fork Road, Blacksburg, VA 24061-0342, USA XX FH Key Location/Qualifiers FH FT source 1..5090 FT /organism="Actinobacillus pleuropneumoniae" FT /strain="J45" FT /serotype="5a" FT /mol_type="genomic DNA" FT /db_xref="taxon:715" FT CDS 400..1365 FT /codon_start=1 FT /transl_table=11 FT /gene="cps5A" FT /product="putative glycosyltransferase" FT /note="region 2 capsular polysaccharide biosynthesis gene; FT Cps5A" FT /db_xref="GOA:O68659" FT /db_xref="InterPro:IPR001296" FT /db_xref="UniProtKB/TrEMBL:O68659" FT /protein_id="AAC26630.1" FT /translation="MSSIMTRPIINHVMSRDIQSGIFSSILEYFTDFGSNEFQHIVSVS FT PIPEAKVYHYHRPHLEEKLLPNSVCTVHHDLNDPDPWHAKYRFIPRYMEAGAIICLNYT FT QKEILISQGLPEHKLFVIPHGYNQKVLFPKKIKEISSTDKITLGIASRRYGRRVKGDAY FT LFELAKRLNPDHFKFIFVGKDRQYSALEMQDLGFEAQVYERLPYRMFQSFYNNIDVLLM FT CSSHEGGPANIPEALATGTPIFSSNIGIPKDVVINYKNGLILTLDPDIDAEQINFICLE FT KPNIFENILDFSLKQSPSLAISWEKCIQQNILVYKKIIKG" FT CDS 1368..2948 FT /codon_start=1 FT /transl_table=11 FT /gene="cps5B" FT /product="region 2 capsular polysaccharide biosynthesis FT protein" FT /note="Cps5B" FT /db_xref="GOA:O68660" FT /db_xref="InterPro:IPR007833" FT /db_xref="UniProtKB/TrEMBL:O68660" FT /protein_id="AAC26631.1" FT /translation="MSISILVPDSLHINKRNFSSFFSWIEKNKINIHFENNNKDWISLY FT GFYDSKLGILYEKIDILTKIEEEELFAFCVYDLNIFNICRAELLSLVATRPEWYNEDYP FT NNLREIYKKLYTNNRSELLQNMAAAWYWVDFWKKRLSELKQFSHCCVFSGGLIYQKSLI FT ELLKYTPTKVMVMESLFTGNEYYCEERYSSIANNSDIKHLAIFNSYKKTFSSKSEYDKE FT RMKAINKFLLMKNKNVQQPTDSEILVFKQQKPIITIIGQVINDFSVLEYKGRGLSTIKI FT YKELISKLSENGFNVVLKTHPWEEKKNNIRTSLTKNIIEEFLKNLTENQQECIKIVDHY FT SIKKLFKQSDFIISLNSQGLLEAAFDGIKPIQLGNAFYGKKGFTYDYDFLDIDQLVNDL FT VVNKLTPTLSLEEFDLFEEFITILLQKHAVSIHASGVSVLSRIFNLPTIIPLVENVPKE FT KSKTTLPTQKDVVKKENTTIVNMVELPKVVPQSDKNRKYQKFRNNPRQFFADSRNPVIR FT SLMYFFPYK" FT CDS 2963..4111 FT /codon_start=1 FT /transl_table=11 FT /gene="cps5C" FT /product="region 2 capsular polysaccharide biosynthesis FT protein" FT /note="Cps5C" FT /db_xref="UniProtKB/TrEMBL:O68661" FT /protein_id="AAC26632.1" FT /translation="MLKKYQPFDLRKINEGHSSNAKLVLHSEACNIDAKISKFFCSQDD FT INLENFIATFTDNYKAPEVYTAILKNCCITPRAPKLPRYRGIFFEENNIPYYVFNFLSS FT EAKKRFNDAKYFYLEDDDRISLDLEKVNKIHISGLSVWFYTFRNVDHFLRECLPSLVTL FT KQMGYDFSALNFICPEILPDIIDFLCDFGIDRRNIISLDYQWLSFDELIIPCFSTFGHL FT HTPTKYYTEITNYPIINSKSREDIKRIYVSRANAKMRRVINEDVIVSELIARGFSIIEP FT GNYSKLEQREIFSNAEVVIGPHGMGIANSVFSKNLKAILEIMNTDYHRISYFRTAQLKG FT CLYAAYYVNPLPLDASYNNNYGDIVIHKTKFLNFLDSILKEI" FT CDS 4160..5020 FT /codon_start=1 FT /transl_table=11 FT /gene="cps5D" FT /product="3-deoxy-D-manno-2-octulosonic acid 8-phosphate FT synthetase" FT /note="Cps5D; similar to kdsA; region 2 capsular FT polysaccharide biosynthesis gene" FT /db_xref="GOA:O68662" FT /db_xref="HSSP:1G7V" FT /db_xref="InterPro:IPR006269" FT /db_xref="UniProtKB/Swiss-Prot:O68662" FT /protein_id="AAC26633.1" FT /translation="MTILNKIVKVGNIEVANDKPFTLFGGMNVLESRDMAMRVCEQYVE FT VTNKLGVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKDTFGVSIITDVHEIYQC FT KPVAEVVDIIQLPAFLARQTDLVEAMARTGVVINVKKPQFLSPGQMGNIVEKIAECGNE FT NVILCDRGTNFGYDNLVVDMLGFNIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRRDQ FT VTELARSGMAIGLAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDDLVKSFEE FT IDTSR" XX SQ Sequence 5090 BP; 1849 A; 701 C; 836 G; 1704 T; 0 other; aagcttgagc aggcagccaa actagcaacc agccccaaag aaaggagtaa tctaagtttg 60 atgagtttca tctaatttct cttcaatata ttaaggaata acaactatat aggtatgtct 120 taaaatccac ataaagattg attttaataa gttacctaat caagagaaat taaatataag 180 aaatttacaa acaaattaaa aaatgtattt tttttaaaaa aaagtaaatc aagaggggcg 240 ttatacagat aaacattata atttaaaagc catataaaat acggagtttc ccctagatag 300 ttgataaatt tctcatttat atttatgaaa ttccgatgaa aaatttatca actatctagg 360 gtaactccat aacgtattcg tatttcagga gtatttttaa tgtctagcat aatgactcgt 420 cctataatta atcatgtaat gtctagagat attcaaagtg gcatatttag ttctatttta 480 gaatatttta ctgattttgg ttccaatgaa tttcaacata ttgtcagtgt atctccaata 540 cctgaagcta aagtttatca ctatcaccgt ccacacctag aagaaaaatt attacctaat 600 tctgtttgta cagtacatca tgacctcaat gatccagatc cttggcatgc taagtataga 660 tttattccta gatatatgga agctggggct ataatttgtt taaattacac tcaaaaagaa 720 attttaatat ctcagggact tccggaacat aagttatttg tgattcctca cggatataat 780 caaaaagtat tatttcctaa gaaaattaaa gaaatatcaa gtacagataa aattacctta 840 ggaattgctt cacggagata tggtagaaga gtaaaaggag atgcatattt atttgaatta 900 gcaaaaagat taaatccaga ccattttaaa tttatttttg ttggtaaaga tagacaatat 960 agtgccttag aaatgcaaga tctaggattt gaagctcaag tatatgaaag attgccatat 1020 agaatgtttc aaagttttta taataatatt gatgtactac ttatgtgtag tagtcatgaa 1080 ggtggacctg caaatatccc cgaagcatta gctactggga cacctatatt ttcatctaac 1140 ataggtatac ctaaggatgt tgttattaat tataagaatg ggttgattct aaccttagat 1200 ccagatatag atgctgaaca gattaatttt atttgccttg aaaaaccaaa tatatttgaa 1260 aatatattag atttttcact aaaacagtct ccaagtttag caatttcttg ggagaaatgt 1320 attcaacaaa atattttagt ttataaaaaa ataattaagg gttaattatg tccatttcta 1380 ttctagtacc tgattcttta cacattaaca aaagaaactt tagttcattc ttcagttgga 1440 ttgagaaaaa taaaataaat atccattttg aaaataataa taaagattgg atttcattat 1500 atggttttta cgattcaaaa ttgggtattc tatatgagaa aatagatatt cttactaaga 1560 ttgaagaaga ggaattattt gctttttgtg tttatgatct aaatattttc aatatttgta 1620 gagctgaatt attatcttta gtagccacaa gacccgaatg gtataatgaa gattatccta 1680 ataacttaag agaaatatac aaaaaactct atactaataa tcgaagtgaa ttattgcaaa 1740 acatggctgc tgcttggtat tgggttgatt tctggaaaaa acgcctatct gagttaaaac 1800 aattctctca ttgttgtgta ttttcaggag gtttaattta tcaaaaatct ttgattgagt 1860 tattaaaata tactccaact aaagttatgg ttatggaaag cctatttaca ggaaacgaat 1920 attattgtga ggaacgttat tcatcaattg ctaataatag cgatattaaa catttagcta 1980 tttttaactc ttataaaaaa acatttagtt caaaaagtga atatgataag gaacgaatga 2040 aagctattaa taagttccta ttaatgaaaa ataagaatgt ccaacaacct actgattctg 2100 aaatattagt atttaaacaa caaaaaccaa taattactat tattggacaa gtgataaatg 2160 atttttcagt cctagaatat aaagggagag gactatcaac aattaaaatc tataaagaac 2220 ttatatctaa actatcagag aatggattta atgtagtatt aaaaactcac ccttgggaag 2280 agaaaaaaaa taatatccgt acatctttaa ctaaaaatat aatagaagaa tttctaaaaa 2340 atctaactga gaatcaacaa gaatgtataa aaatagttga tcactattca ataaagaaat 2400 tatttaaaca atctgatttt attattagtt taaattctca agggctcctt gaagctgcat 2460 ttgatggtat aaaacctata cagttaggta atgcttttta tggaaaaaaa ggattcacgt 2520 acgattatga ctttttagat attgatcaat tggtaaatga cttagtagta aataaactta 2580 ctccaacact atctttagaa gagtttgatt tgttcgaaga gttcattact atattattac 2640 aaaagcatgc tgtttctatt cacgcctctg gcgtaagtgt tttatctaga atatttaatt 2700 tacctactat tataccatta gtagaaaatg tccctaagga gaagtctaaa acaacattac 2760 ctactcaaaa agatgtggta aaaaaggaaa atacaacaat tgttaatatg gttgagttac 2820 ctaaagtagt tccacaaagt gataagaata ggaaatatca aaaatttaga aacaatcctc 2880 gacaattctt tgcagattct aggaatccag ttattagaag tttaatgtat tttttccctt 2940 ataaataata taggtctaat ttatgttaaa aaaatatcag ccttttgatt taagaaaaat 3000 aaatgaaggc cactctagta atgctaagtt agttttacat tctgaggcct gtaatataga 3060 tgctaaaatc tctaagtttt tctgttcaca agatgacatt aatttagaaa actttattgc 3120 aacatttact gataactata aagcaccaga agtatatacg gcgattttaa agaattgttg 3180 tattacacct agagcaccta agctaccaag atatcggggg atattttttg aagaaaataa 3240 tattccctac tatgtattta attttttaag ttctgaagct aaaaaaagat tcaatgatgc 3300 taaatatttt tacttagaag atgatgatag aatttccctt gatttagaga aagttaataa 3360 aattcatatt tcagggctta gtgtatggtt ttatactttt cgtaatgtag atcatttttt 3420 acgagaatgt ttgccttcat tagttacatt aaaacagatg ggatatgact ttagtgcttt 3480 gaactttatt tgtcctgaaa tattacctga tattattgat tttctatgtg attttgggat 3540 agatagaaga aatattatca gccttgatta tcaatggtta tcgtttgatg aattaattat 3600 tccttgtttt agtacgtttg gacatctgca tacaccaaca aaatattata ctgaaattac 3660 taattaccct attattaact ctaaatctag agaggatata aaaagaattt atgtatctag 3720 agcgaatgct aaaatgcgta gagtcataaa tgaagatgtt attgtttccg aactgattgc 3780 taggggcttt tctatcattg aacctggaaa ttattctaag ctggaacaac gagagatttt 3840 ttcaaatgcg gaagtagtta ttggtccaca tgggatggga atagctaata gtgttttttc 3900 gaaaaatcta aaagcaattt tagaaataat gaatactgac tatcacagaa ttagttattt 3960 tagaactgct caattaaaag gttgtttata tgctgcttac tatgtaaatc ctttaccatt 4020 agatgcttca tataataata attatggaga tatagttata cataaaacaa aatttctcaa 4080 ttttttagat tcaatattaa aagaaatata attaaagaac tttaatttta gatttacttt 4140 actaagtgga attaaaatca tgacaatttt aaataaaatc gtaaaagttg gcaatattga 4200 agtggcgaat gacaagccgt ttactttatt cggtggtatg aacgtattag aaagccgtga 4260 tatggcaatg cgtgtctgtg aacaatacgt agaagtgacc aacaaactgg gtgtgcctta 4320 tgtatttaaa gcctctttcg ataaagcgaa tcgctcgtca attcactctt accgaggacc 4380 gggtatggaa gaaggtttaa aaatcttcca agaattaaaa gatacgttcg gtgtgagcat 4440 tattactgac gtacacgaaa tttatcaatg taaaccggta gcggaagtgg tggatattat 4500 ccagttaccg gcattcttag ctcgccaaac agatttagtc gaagcaatgg cacgtaccgg 4560 tgtggtgatt aacgtgaaaa aaccacaatt tttaagcccg ggtcaaatgg gtaatatcgt 4620 agaaaaaatt gcagaatgcg gtaacgaaaa cgtaattctt tgtgaccgtg gtactaactt 4680 cggctacgat aatttagtgg tggatatgct cggctttaac attatgaaaa aagtatcaaa 4740 aggctgtccg gtcatttttg acgtgactca ctcactacaa tgccgtgacc cgttcggtgc 4800 agcatcaggc ggtcgccgtg atcaagtaac cgaattggct cgcagcggta tggcaattgg 4860 tttagccggt ttattccttg aagcgcatcc ggatccgaac agtgcgaaat gtgacggtcc 4920 gtcagcccta ccgttatcaa aattagaagc attcgttagc caaatgaaag cgattgatga 4980 tttagttaaa tcatttgaag aaattgatac atcaagataa gaaatacttc ccctcttaat 5040 tagaggggat tttttatgag gtttttatga agtttactat tattatcccg 5090 // |
|