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EBI DbfetchID AF051376; SV 1; linear; genomic DNA; STD; PRO; 3396 BP. XX AC AF051376; XX DT 17-MAR-1998 (Rel. 55, Created) DT 17-MAR-1998 (Rel. 55, Last updated, Version 1) XX DE Methanobacterium wolfei MwoI restriction endonuclease (mwoIR) and MwoI DNA DE modification methyltransferase (mwoIM) genes, complete cds. XX KW . XX OS Methanothermobacter wolfeii OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanobacteriaceae; Methanothermobacter. XX RN [1] RP 1-3396 RX DOI; 10.1016/0378-1119(89)90354-5 RX PUBMED; 2663652. RA Lunnen K.D., Morgan R.D., Timan C.J., Krzycki J.A., Reeve J.N., RA Wilson G.G.; RT "Characterization and cloning of MwoI (GCN7GC), a new type-II RT restriction-modification system from Methanobacterium wolfei"; RL Gene 77(1):11-19(1989). XX RN [2] RP 1-3396 RA Zhang B.-H., Wilson G.G.; RT ; RL Submitted (26-FEB-1998) to the EMBL/GenBank/DDBJ databases. RL New England Biolabs Inc., 32 Tozer Road, Beverly, MA 01915, USA XX FH Key Location/Qualifiers FH FT source 1..3396 FT /organism="Methanothermobacter wolfeii" FT /mol_type="genomic DNA" FT /db_xref="taxon:145261" FT RBS 85..89 FT CDS 96..1187 FT /codon_start=1 FT /transl_table=11 FT /gene="mwoIR" FT /product="MwoI restriction endonuclease" FT /function="cleaves ds DNA sequence GCNNNNNNNGC to yield FT GCNNNNN/NNGC" FT /note="R.MwoI" FT /db_xref="GOA:O59646" FT /db_xref="UniProtKB/Swiss-Prot:O59646" FT /protein_id="AAC05699.1" FT /translation="MKNPKFTENQKEIEKEEFEFLQKLNFIIKESLELFNTNLKNSMKF FT INYITLPIIMASIESKSFNPFSEIIEKHIAFILNSKMNSLGYKFLPLGYSSDLTYENDN FT SIIHIDIKTANLENPSDFKDTVPLGINQSSYPGVLDCKIRGKNIKADCKKIKVYPNIPT FT TYNNKLTITNALLFIYPDYKEIIDEIREDYIAIRELISINLKDILTPIEGSLEEFLNYK FT PSNEKKRLEPILDNIVRGYFIHDKLRHEFSENVEKDLEEFEKKIIGIAKKLKEREIKPV FT AILSISIPNGELAPHYDDEIVSGKSWGSSFRYHYKKSGNSVFKGLDNKASRAVFLHINK FT EYLPVLKKYFDPITVYELTEKRL" FT RBS 1187..1189 FT CDS 1195..3201 FT /codon_start=1 FT /transl_table=11 FT /gene="mwoIM" FT /product="MwoI DNA modification methyltransferase" FT /function="recognizes ds DNA sequence GCNNNNNNNGC and FT methylates one cytosine on each strand at the N-4 position" FT /note="M.MwoI; N4-cytosine DNA MTases beta-family member" FT /db_xref="GOA:O59647" FT /db_xref="InterPro:IPR017985" FT /db_xref="UniProtKB/Swiss-Prot:O59647" FT /protein_id="AAC05700.1" FT /translation="MVKGKILFGDVFSALRCLEDNSISVALTSPPYWRQRDYGFKGQIG FT REKTPEEYIGRLIVIFRELRAKLKDDGVFFLNIGDKYKNRYGKSHLLQIPYRLAAHMIK FT DGWKLLDIIIWYKPNHMPSSVKDRFTNTYEPVLVFGKSDENIYTKKHPVLKIPLQQTKW FT KHTAVFPEKLVSSLLSRCNLKDGDYILDPFAGTGTTGAVVKKMKYQLYPKDLNVILIEK FT GKKFLDIITERTGIKEIKELKSSEYTWEPVNDKLAFSEDKPLIIIEDTHGETFIAKNSE FT EFSRIIMGMLSEEFQDFHREDAVYFFGVKNWKLSDLVLPGLLIDHGFILRNMIIIEDGS FT SWYPVFMLVKDTTRVNYKFYIDRIRKKPKTVLPEKWNQEDFIGLIVNDNLSKKPRKGEV FT VDIISTYSQDNFPKIVAVSWEDDNISLELCLNPRKDEFIMESLQFTCPHCGTQLIDTYD FT PLGDNICYNCQKEIYGKNSLPILKESKEIIESLESVENGEYQVGENIKPQYQKRCKESK FT SKFAGMERMNWGASPGARKTIIGDSFSKMRLYRLDQPTIARYLNIYMKKNDLRIKDITQ FT ALPPEYKHTVGHWFRKDFGGSIPLPEDVTLLEEILKLDKEFARILKRSVLKLQTVKHSL FT KGKNPGDFLELEENKLKEYLTKTYMPPSYYIKK" XX SQ Sequence 3396 BP; 1287 A; 562 C; 587 G; 960 T; 0 other; ggatccaggt tcatggacat gatgataaat ccaaaaggct acgaaaattt tgacatgaaa 60 tgaagtctac attacagttt tagtaggtgc aaaaaatgaa aaatcccaaa ttcacagaaa 120 accaaaaaga aattgaaaaa gaagaatttg aatttctaca aaagctaaac tttataataa 180 aagaaagttt ggaattattt aatacaaatc taaagaattc aatgaagttt attaattata 240 taacattgcc tataatcatg gcaagcatag aatcaaaatc ttttaatcca ttctctgaga 300 ttatagaaaa acatatagca ttcattttaa attctaaaat gaattcactt ggttataaat 360 ttttaccatt aggttattct tctgatttaa catatgaaaa tgataatagt attattcata 420 tagatatcaa aactgctaat cttgaaaacc cttcagattt taaagacact gtacctcttg 480 gaattaacca atcaagttat ccgggagttt tagattgcaa aataagaggt aagaatataa 540 aggctgattg taaaaaaata aaggtatacc caaatattcc caccacttat aataataagc 600 ttacaatcac gaatgctctt ctattcatct atcccgacta taaagaaata attgatgaga 660 taagggaaga ttatattgct ataagagagt taattagtat caatttaaaa gacattctta 720 ctccaataga aggctcttta gaagaatttt tgaattataa accatctaat gaaaaaaaga 780 gattagaacc tattttggat aatatagtga gagggtattt tatacatgat aaactacgtc 840 atgaattctc agaaaacgtt gaaaaggact tggaagaatt tgagaaaaag attattggaa 900 tagcaaaaaa gttaaaggaa cgagaaataa aacccgttgc catattatcc atttctattc 960 ccaatggaga attagcgcct cattatgatg atgaaatagt gagtggcaag agctggggat 1020 cttccttcag atatcattac aagaaaagcg gtaactcagt tttcaaagga ttagataaca 1080 aggcatcccg tgccgtgttc ctacacatca acaaagaata cttacctgtt ttaaaaaaat 1140 attttgatcc cattaccgta tatgagctaa cagagaaaag gttatagtgg tagtatggtt 1200 aaaggaaaga ttttatttgg ggatgttttt agtgcattaa gatgtttaga ggataatagt 1260 atttctgttg cattaacttc tcctccctac tggagacaaa gagattatgg gtttaaagga 1320 caaattggta gagaaaagac tccggaagag tatataggga gattaatcgt tatattcagg 1380 gagttaaggg ctaagctaaa ggatgatgga gtgttcttcc taaatatagg tgataagtat 1440 aagaataggt atggtaaatc ccatcttctt cagatccctt ataggcttgc agcacatatg 1500 atcaaagatg gttggaagct tttagatata attatatggt ataaacccaa ccatatgcct 1560 tcatcagtta aagacagatt cacaaacact tatgaacccg tcctagtttt cgggaaatcc 1620 gatgaaaaca tttacacaaa aaaacaccca gtcctcaaaa taccactgca gcaaacaaaa 1680 tggaagcata cagcagtttt tcctgagaaa ctggtttcat ctttactctc aaggtgcaac 1740 cttaaagatg gtgattacat tttagaccct ttcgcaggga caggaaccac aggggcagtg 1800 gtaaaaaaaa tgaagtatca gctgtatccc aaagatttga atgttatact aatcgagaag 1860 ggaaaaaaat ttttagatat tatcactgaa agaactggta tcaaggaaat taaagagctt 1920 aaatcttctg aatatacatg ggaaccagtt aatgataaat tagccttttc agaggataaa 1980 cctcttataa tcatcgagga tactcatggt gaaacattca tcgctaaaaa cagtgaagag 2040 ttctcccgta taataatggg aatgctttca gaagaattcc aggattttca tagagaggat 2100 gctgtctact ttttcggtgt aaaaaactgg aaattatcag accttgtttt accaggatta 2160 ttaatagacc atggctttat acttcgaaat atgataataa ttgaagacgg ctcttcatgg 2220 tatcctgtct ttatgctggt aaaagacaca acaagggtca actacaaatt ttatatagac 2280 aggattagaa agaaaccaaa aaccgtacta ccagaaaaat ggaaccaaga agactttata 2340 ggcctcatcg taaatgataa cctctctaaa aaacccagga aaggagaagt tgttgacata 2400 atatcaacat acagccagga taatttccct aaaatagtcg ctgtatcctg ggaggacgac 2460 aatatttctc ttgaattatg cctaaatccc cgaaaagatg aattcatcat ggaatcacta 2520 caatttacat gtcctcactg tgggacacaa cttatagaca cctatgaccc cttaggggat 2580 aacatctgtt ataactgcca aaaagagata tacggaaaaa actccctccc catcctaaaa 2640 gaatcaaaag aaatcataga atccctagaa tcagttgaaa atggagaata tcaagtcgga 2700 gaaaacataa aaccacagta tcaaaaaaga tgcaaggaaa gtaaaagtaa attcgcaggc 2760 atggaacgaa tgaattgggg cgcttcacca ggagcaagaa aaaccataat aggcgactcg 2820 ttctccaaga tgagattata tcgattagat caaccaacca tagccagata ccttaatatt 2880 tatatgaaaa agaatgacct gagaattaaa gacattaccc aagccttacc accagaatac 2940 aaacatacag tagggcattg gttcagaaaa gattttgggg gatccatacc actcccagag 3000 gacgtgaccc tattagagga aatcctcaaa ctggacaaag aattcgccag aatcctaaaa 3060 agaagtgtcc tcaaattaca gacagtaaaa cattccctga agggtaaaaa tccaggagat 3120 ttccttgaac ttgaagaaaa caaacttaag gaatatttaa caaaaacata catgccacct 3180 tcttattaca taaaaaaatg aaaaggttct tttttcttta attaaatctt atttaaatgt 3240 ccttttaagt attgcctctt tttctggagt tttattgatc ttctagcatg atatttcatc 3300 cacagtacag tatttaatcc gtctactatg ctcaggatgg tgtaaatatc cattctcctt 3360 tttctggcgg ctctactgca ctaggactgt gtaatc 3396 // |
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