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EBI DbfetchID AF051374; SV 1; linear; genomic DNA; STD; PRO; 4241 BP. XX AC AF051374; XX DT 17-MAR-1998 (Rel. 55, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Fusobacterium nucleatum adenylosuccinate synthetase (purA), FnuDI DE restriction endonuclease (fnuDIR), and FnuDI DNA modification DE methyltransferase (fnuDIM) genes, complete cds. XX KW . XX OS Fusobacterium nucleatum OC Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium. XX RN [1] RP 1-4241 RA Zhang B.-H., Wilson G.G.; RT ; RL Submitted (26-FEB-1998) to the EMBL/GenBank/DDBJ databases. RL New England Biolabs Inc., 32 Tozer Road, Beverly, MA 01915, USA XX FH Key Location/Qualifiers FH FT source 1..4241 FT /organism="Fusobacterium nucleatum" FT /strain="D" FT /mol_type="genomic DNA" FT /db_xref="taxon:851" FT RBS 572..577 FT CDS 583..1860 FT /codon_start=1 FT /transl_table=11 FT /gene="purA" FT /product="adenylosuccinate synthetase" FT /note="PurA; similar to the Escherichia coli product FT encoded by PIR Accession Number A31965; similar to the FT Dictyostelium discoideum product encoded by PIR Accession FT Number A38617" FT /db_xref="GOA:O68581" FT /db_xref="HSSP:1DJ2" FT /db_xref="InterPro:IPR001114" FT /db_xref="UniProtKB/Swiss-Prot:O68581" FT /protein_id="AAC05693.1" FT /translation="MAGYVVVGTQWGDEGKGKIIDVLSEKADYVVRFQGGNNAGHTVVV FT NGEKFILQLLPSGVLQAGTCVIGPGVVVDPKVFLDEIDRIEKRGAKTDHVIISDRAHVI FT MPYHIEMDKIRESVEDRIKIGTTKKGIGPCYADKIARDGIRMSDLLDLKQFEEKLRANL FT KEKNEIFTKIYGLEPLDFDKIFEEYKGYIEKIKHRIVDTIPIVNKALDENKLVLFEGAQ FT AMMLDINYGTYPYVTSSSPTLGGVTTGAGVSPRKINKGIGVMKAYTTRVGEGPFVTEIK FT GEFGDKIKGIGGEYGAVTGRPRRCGWLDLVVGRYATEINGLTDIVITKIDVLSGLGKLK FT ICTAYEIDGKVYDYVPADTKSLDKAIPIYEELDGWDEDITQIKKYEDLPVNCRKYLERV FT QEILACPISVVSVGPDRSQNIHIREI" FT RBS 1973..1976 FT CDS 1983..2837 FT /codon_start=1 FT /transl_table=11 FT /gene="fnuDIR" FT /product="FnuDI restriction endonuclease" FT /function="cleaves ds DNA GGCC to yield GG/CC" FT /note="R.FnuDI; similar to homoschizomers R.NgoPII and FT R.MthTI" FT /db_xref="GOA:O68582" FT /db_xref="InterPro:IPR019046" FT /db_xref="UniProtKB/TrEMBL:O68582" FT /protein_id="AAC05694.1" FT /translation="MKNNKNIIDAIINIVKNPILELKEYSISHNRANSMGEALEEYIKD FT VFSGTLFEKDENKRMEIISEVFSYLGNTNNPPDSILRGGDAIEVKKIENKSSSLALNSS FT YPKAKLYSNSSMITDACRSCEKWEEKDIIYTIGTCEKNKLTSLIFVYGEDYAAENKIYE FT NVKNKIKLGIETINDLEFSETNEIGRVNRVDPLGITYFRIRGMWGIENPVKVFDYIYKR FT DDSKQFNFMALINEDKYLSFSNTKELEDLEKENKNLEIKDVRIKNPNNPAQLKKAKLIT FT FKI" FT RBS 2839..2843 FT CDS 2849..3883 FT /codon_start=1 FT /transl_table=11 FT /gene="fnuDIM" FT /product="FnuDI DNA modification methyltransferase" FT /function="recognizes ds DNA sequence GGCC and methylates FT the internal cytosine to yield GGmCC" FT /note="M.FnuDI; 5-cytosine DNA MTase family member; similar FT to homomethylomers M.HaeIII and M.NgoPII" FT /db_xref="GOA:P34906" FT /db_xref="HSSP:1DCT" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P34906" FT /protein_id="AAC05695.1" FT /translation="MKLLSLFSGAGGLDLGFERAGFEIIVANEYDKTIWETYEKNHKAK FT LIKKDIREILSEELPKSDGIIGGPPCQSWSEAGSLRGINDPRGKLFYEYIRILKDIQPK FT FFLAENVKGMLSKRNTEAVKDIIKEFEEAGYNVFIKLLNAFDYGVAQDRERVFYVGFRK FT DLNISNFEFPYPISEKERKYLKDSIWDLKDNALPGKDKNKTNADDCIVENHEYLTGSYS FT TIFMSRNRVRQWEQPAFTVQASGRQCQLHPQAPTMIKIDKNMYKFVAGKENLYRRLSIR FT ECARIQGFPDTFKFYYTSLEDGYKMVGNAVPVDLAYIIAKRIKETLTDKEKIKKEIRQK FT TLFD" XX SQ Sequence 4241 BP; 1785 A; 408 C; 759 G; 1289 T; 0 other; tagctgataa tgaaatatct gaaatgtata taaatttccc agatccttgg gaaggaacag 60 agaagaatag aattatccaa gaaagattat ttaaaactct ggataagatt atgaaaaaag 120 atggaatgct atactttaaa actgaccatg atatctatta tagtgatgtt ttagagttag 180 taaaaacttt ggataattat gaagtaattt atcatacctc tgatttacat aattcagaaa 240 aagcagaaaa taatataaaa acagagtttg agcaattatt tttacataaa cataataaaa 300 atataaatta taatgaaatt aagaaaataa tctaagtttt aattttaatt aaaaactaaa 360 aatagttcat tactgagtag atttattaac gataaaaaat caagaattcg ctgcaaattc 420 aaccaactcg ctaacaagtt agctcaaaca tgttgagatt tgctcggctc attctatttg 480 attttttatc taaaatctac attcgtaatt cacttatttt ttatcttatt gtaaatttta 540 ataatattta tctaaaaata aaagggaata taggaggaag aaatggctgg ttatgttgta 600 gtaggtactc aatggggaga cgaaggaaaa ggtaaaatca tagatgtttt atcagaaaaa 660 gctgattatg tagtaagatt tcaaggtgga aataatgctg gacatacagt tgtagtaaat 720 ggagaaaaat ttattctaca acttcttcca tcaggtgtac ttcaagctgg tacttgtgta 780 attggaccag gtgttgttgt tgacccaaaa gttttcctag atgaaataga tagaatagaa 840 aaaagaggtg ctaagacaga ccatgttatt ataagtgata gagcacatgt tattatgcct 900 tatcacattg aaatggataa aataagagaa agtgttgaag atagaataaa aataggaaca 960 actaaaaaag gaattggacc ttgttatgcc gataaaattg caagagatgg tataagaatg 1020 tctgatttac tagatttaaa acaatttgaa gaaaaattaa gagctaattt aaaagaaaaa 1080 aatgaaatat ttacaaaaat ttatggttta gaaccacttg actttgataa gatttttgaa 1140 gaatacaaag gatatattga aaaaataaaa catagaatag ttgatactat tccaatagta 1200 aataaagcat tagatgaaaa taaacttgta ctttttgaag gagcacaagc tatgatgctt 1260 gatatcaact atggaactta tccttatgtt acttcatcat ctcctacact tggaggagtg 1320 acaacaggag caggagtttc tcctagaaaa attaataaag gaataggagt aatgaaagcc 1380 tatacaacaa gagttggaga aggtccattt gtaactgaaa taaaaggaga atttggagat 1440 aaaatcaaag gaattggtgg agaatatgga gctgttactg gtagaccaag aagatgtggg 1500 tggcttgatt tagttgtggg aagatatgcc actgaaataa atggcttaac tgacatagtt 1560 ataactaaaa tagatgtttt gagtggatta ggaaaattaa aaatttgtac tgcctatgaa 1620 atagatggaa aagtttatga ttatgttcca gctgatacaa aatctttgga caaggctata 1680 cctatatatg aagaacttga tggttgggac gaagatataa ctcaaatcaa aaaatatgaa 1740 gatttaccag taaattgtag aaaatatttg gaaagagtac aagagatttt agcttgtcct 1800 atatctgttg tttctgttgg gccagataga agtcaaaata tacatataag agaaatatag 1860 aaaaaacaat ctacataaaa aacagttcat taatattagg atagcttagg gaaacataaa 1920 gaactaatgc tatgtttatt tgatgagctg tttttttata aaatttaaat aagagggaaa 1980 atatgaaaaa taacaaaaat attatagatg caattattaa tatagtaaaa aatcctattt 2040 tagaattaaa agaatattct ataagtcaca atagggcaaa tagtatgggt gaagcacttg 2100 aagaatatat aaaagatgtt ttttcaggta cactttttga aaaagatgag aataaaagga 2160 tggaaattat ttcagaggta tttagctatt tgggaaatac caataatcca ccagatagta 2220 ttttaagggg aggagatgcc atagaggtaa agaagattga gaataaaagt tcatctttag 2280 cattaaatag ctcatatcca aaagcaaaat tatattcaaa tagttctatg ataacagatg 2340 cttgtcgaag ttgtgaaaaa tgggaagaga aagatattat ttatacaata ggtacttgtg 2400 aaaaaaataa attaacttct ttgatatttg tatatgggga agattatgca gcagaaaata 2460 aaatatatga aaatgtaaaa aataaaataa aattaggaat tgaaacaata aatgatttag 2520 agttttcaga aacaaatgaa ataggaagag taaacagagt tgacccatta ggtataactt 2580 attttagaat aagaggcatg tggggtattg aaaatccagt aaaagtattt gattatattt 2640 acaaaagaga tgatagtaaa caatttaatt ttatggcact aattaatgaa gataaatatc 2700 tttctttttc caatacaaaa gagttagaag atttagaaaa agaaaataaa aacttggaaa 2760 taaaagatgt tagaataaaa aatccaaata atccagcaca attaaaaaaa gcaaaattaa 2820 ttacatttaa aatatagtag gagatataat gaaattatta agtttattca gtggagcagg 2880 aggcttagat ttaggttttg aaagagcagg atttgagata attgttgcta atgaatacga 2940 taaaacaata tgggaaactt atgaaaaaaa tcataaagca aaattgataa aaaaagatat 3000 tagggaaatt ttatctgaag aactgcctaa aagcgatgga attataggtg gacctccttg 3060 tcaatcttgg agtgaagctg gttcattaag aggaataaat gatccaagag gaaagttatt 3120 ttatgaatat attagaatat taaaagatat tcaacctaaa ttttttttgg cagaaaatgt 3180 aaaaggaatg ttatcaaaga gaaatactga agcagtaaaa gatataatta aagaatttga 3240 agaagcaggg tataatgttt ttataaaatt acttaatgct tttgattatg gagtagctca 3300 agatagggaa agagtgtttt atgttggttt tagaaaagat ttaaatataa gtaattttga 3360 atttccttat cctattagtg aaaaagaaag aaaatattta aaagatagta tatgggattt 3420 gaaagacaat gccttacctg gaaaagataa aaataaaaca aatgctgatg attgtattgt 3480 tgaaaatcat gaatatttaa caggaagtta ttcaactatt tttatgagta gaaatcgtgt 3540 aagacaatgg gaacaaccag cttttacagt acaagcctct ggaagacaat gtcaattaca 3600 ccctcaagca cctactatga taaaaataga taaaaatatg tataaatttg ttgctggaaa 3660 ggaaaattta tatagaagat tgagtataag agaatgtgct agaatacaag gttttcctga 3720 tacatttaag ttttactata cttcactaga agatggctat aaaatggttg gaaatgctgt 3780 gcctgtggat ttagcatata taatagcaaa aagaataaaa gaaactttaa cagacaaaga 3840 aaagataaaa aaagaaatta gacaaaaaac tttgtttgat taaataagga gatttatgag 3900 aacattatta ttattaagag gaatacaagc aagtgggaag tccacttgga taaaagaaaa 3960 taacttagaa gcatatacat taagtgctga taatataaga ttaaatattg caaaccctgt 4020 tttaaatgaa gatggttctt atgaaattag tcaaaaatat aataaagtaa cttgggaact 4080 tttatataat tatttagaga tgagaatgca aaatggtgat tttacaataa tagatgctac 4140 ccattctgat ataaaacttt tgaataaata tagagattta gcaaatacat ataaatatac 4200 tatgtactgc ctagagtttg atgtcgcttt agaagaagct t 4241 // |
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